LOCUS Exported 5405 bp ds-DNA circular SYN 20-DEC-2017
DEFINITION synthetic circular DNA
FEATURES Location/Qualifiers
source 1..5405
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 66..423
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 274..409
/label=SV40 ori
/note="SV40 origin of replication"
intron 493..625
/label=chimeric intron
/note="chimera between introns from human beta-globin and
immunoglobulin heavy chain genes"
promoter 670..688
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 698..1630
/codon_start=1
/product="luciferase from the anthozoan coelenterate
Renilla reniformis (sea pansy)"
/label=Rluc
/translation="MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
misc_feature 1633..1662
/label=MCS
CDS 1663..3312
/codon_start=1
/gene="luc"
/product="firefly luciferase"
/label=luciferase
/translation="EDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDAH
IEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAPA
NDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQSM
YTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHAR
DPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYKI
QSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGYG
LTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMSG
YVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESILL
QHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVVF
VDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
polyA_signal 3352..3473
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
promoter 3614..3718
/gene="bla"
/label=AmpR promoter
CDS 3719..4579
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 4750..5338
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 agatcgatct gcgcagcacc atggcctgaa ataacctctg aaagaggaac ttggttaggt
61 accttctgag gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc
121 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
181 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
241 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
301 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
361 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
421 aaaaagcttg attcttctga cacaacagtc tcgaacttaa gctgcagaag ttggtcgtga
481 ggcactgggc aggtaagtat caaggttaca agacaggttt aaggagacca atagaaactg
541 ggcttgtcga gacagagaag actcttgcgt ttctgatagg cacctattgg tcttactgac
601 atccactttg cctttctctc cacaggtgtc cactcccagt tcaattacag ctcttaaggc
661 tagagtactt aatacgactc actataggct agccaccatg acttcgaaag tttatgatcc
721 agaacaaagg aaacggatga taactggtcc gcagtggtgg gccagatgta aacaaatgaa
781 tgttcttgat tcatttatta attattatga ttcagaaaaa catgcagaaa atgctgttat
841 ttttttacat ggtaacgcgg cctcttctta tttatggcga catgttgtgc cacatattga
901 gccagtagcg cggtgtatta taccagacct tattggtatg ggcaaatcag gcaaatctgg
961 taatggttct tataggttac ttgatcatta caaatatctt actgcatggt ttgaacttct
1021 taatttacca aagaagatca tttttgtcgg ccatgattgg ggtgcttgtt tggcatttca
1081 ttatagctat gagcatcaag ataagatcaa agcaatagtt cacgctgaaa gtgtagtaga
1141 tgtgattgaa tcatgggatg aatggcctga tattgaagaa gatattgcgt tgatcaaatc
1201 tgaagaagga gaaaaaatgg ttttggagaa taacttcttc gtggaaacca tgttgccatc
1261 aaaaatcatg agaaagttag aaccagaaga atttgcagca tatcttgaac cattcaaaga
1321 gaaaggtgaa gttcgtcgtc caacattatc atggcctcgt gaaatcccgt tagtaaaagg
1381 tggtaaacct gacgttgtac aaattgttag gaattataat gcttatctac gtgcaagtga
1441 tgatttacca aaaatgttta ttgaatcgga cccaggattc ttttccaatg ctattgttga
1501 aggtgccaag aagtttccta atactgaatt tgtcaaagta aaaggtcttc atttttcgca
1561 agaagatgca cctgatgaaa tgggaaaata tatcaaatcg ttcgttgagc gagttctcaa
1621 aaatgaacaa atgtcgacgg atccttcaac ttccctgagc tcgaagacgc caaaaacata
1681 aagaaaggcc cggcgccatt ctatcctcta gaggatggaa ccgctggaga gcaactgcat
1741 aaggctatga agagatacgc cctggttcct ggaacaattg cttttacaga tgcacatatc
1801 gaggtgaaca tcacgtacgc ggaatacttc gaaatgtccg ttcggttggc agaagctatg
1861 aaacgatatg ggctgaatac aaatcacaga atcgtcgtat gcagtgaaaa ctctcttcaa
1921 ttctttatgc cggtgttggg cgcgttattt atcggagttg cagttgcgcc cgcgaacgac
1981 atttataatg aacgtgaatt gctcaacagt atgaacattt cgcagcctac cgtagtgttt
2041 gtttccaaaa aggggttgca aaaaattttg aacgtgcaaa aaaaattacc aataatccag
2101 aaaattatta tcatggattc taaaacggat taccagggat ttcagtcgat gtacacgttc
2161 gtcacatctc atctacctcc cggttttaat gaatacgatt ttgtaccaga gtcctttgat
2221 cgtgacaaaa caattgcact gataatgaat tcctctggat ctactgggtt acctaagggt
2281 gtggcccttc cgcatagaac tgcctgcgtc agattctcgc atgccagaga tcctattttt
2341 ggcaatcaaa tcattccgga tactgcgatt ttaagtgttg ttccattcca tcacggtttt
2401 ggaatgttta ctacactcgg atatttgata tgtggatttc gagtcgtctt aatgtataga
2461 tttgaagaag agctgttttt acgatccctt caggattaca aaattcaaag tgcgttgcta
2521 gtaccaaccc tattttcatt cttcgccaaa agcactctga ttgacaaata cgatttatct
2581 aatttacacg aaattgcttc tgggggcgca cctctttcga aagaagtcgg ggaagcggtt
2641 gcaaaacgct tccatcttcc agggatacga caaggatatg ggctcactga gactacatca
2701 gctattctga ttacacccga gggggatgat aaaccgggcg cggtcggtaa agttgttcca
2761 ttttttgaag cgaaggttgt ggatctggat accgggaaaa cgctgggcgt taatcagaga
2821 ggcgaattat gtgtcagagg acctatgatt atgtccggtt atgtaaacaa tccggaagcg
2881 accaacgcct tgattgacaa ggatggatgg ctacattctg gagacatagc ttactgggac
2941 gaagacgaac acttcttcat agttgaccgc ttgaagtctt taattaaata caaaggatat
3001 caggtggccc ccgctgaatt ggaatcgata ttgttacaac accccaacat cttcgacgcg
3061 ggcgtggcag gtcttcccga cgatgacgcc ggtgaacttc ccgccgccgt tgttgttttg
3121 gagcacggaa agacgatgac ggaaaaagag atcgtggatt acgtcgccag tcaagtaaca
3181 accgcgaaaa agttgcgcgg aggagttgtg tttgtggacg aagtaccgaa aggtcttacc
3241 ggaaaactcg acgcaagaaa aatcagagag atcctcataa aggccaagaa gggcggaaag
3301 tccaaattgt aacacgtgta attctagagc ggccgcttcg agcagacatg ataagataca
3361 ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa
3421 tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca
3481 acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca
3541 agtaaaacct ctacaaatgt ggtaaaatcg ataagtttaa acagatccag gtggcacttt
3601 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
3661 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
3721 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
3781 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
3841 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
3901 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
3961 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
4021 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
4081 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
4141 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
4201 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
4261 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
4321 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
4381 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
4441 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
4501 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
4561 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
4621 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
4681 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
4741 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
4801 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
4861 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
4921 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
4981 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
5041 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
5101 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
5161 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
5221 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
5281 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
5341 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatggc
5401 tcgac
//