LOCUS Exported 6755 bp ds-DNA circular SYN 28-SEP-2017
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pCMVbeta
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6755)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Sep 28, 2017 from MiaoLingPlasmid
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..6755
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 27..330
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 331..534
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
intron 672..768
/label=SV40 intron
/note="modified SV40 intron with splice donor and acceptor
sites"
CDS 835..3882
/codon_start=1
/gene="lacZ"
/product="beta-galactosidase"
/label=-beta-galactosidase
/translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS
LNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP
FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF
DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR
FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRV
TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP
LLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGL
YVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGA
NHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLS
LPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNP
WSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYL
FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQ
PNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQ
SGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEA
ALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHP
ARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLR
CGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDD
SWSPSVSAEFQLSAGRYHYQLVWCQK"
polyA_signal 3894..4028
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
protein_bind 4163..4179
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(4187..4217)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 4232..4253
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(4541..5129)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(5300..6160)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(6161..6265)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gagttcgagc ttgcatgcct gcaggtcgtt acataactta cggtaaatgg cccgcctggc
61 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
121 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
181 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
241 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
301 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
361 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
421 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
481 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta
541 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac
601 cgggaccgat ccagcctccg gactctagag gatccggtac tagaggaact gaaaaaccag
661 aaagttaact ggtaagttta gtctttttgt cttttatttc aggtcccgga tccggtggtg
721 gtgcaaatca aagaactgct cctcagtgga tgttgccttt acttctaggc ctgtacggaa
781 gtgttacttc tgctctaaaa gctgcggaat tgtacccgcg ggcccatgga tcccgtcgtt
841 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
901 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
961 ttgcgcagcc tgaatggcga atggcgcttt gcctggtttc cggcaccaga agcggtgccg
1021 gaaagctggc tggagtgcga tcttcctgag gccgatactg tcgtcgtccc ctcaaactgg
1081 cagatgcacg gttacgatgc gcccatctac accaacgtga cctatcccat tacggtcaat
1141 ccgccgtttg ttcccacgga gaatccgacg ggttgttact cgctcacatt taatgttgat
1201 gaaagctggc tacaggaagg ccagacgcga attatttttg atggcgttaa ctcggcgttt
1261 catctgtggt gcaacgggcg ctgggtcggt tacggccagg acagtcgttt gccgtctgaa
1321 tttgacctga gcgcattttt acgcgccgga gaaaaccgcc tcgcggtgat ggtgctgcgc
1381 tggagtgacg gcagttatct ggaagatcag gatatgtggc ggatgagcgg cattttccgt
1441 gacgtctcgt tgctgcataa accgactaca caaatcagcg atttccatgt tgccactcgc
1501 tttaatgatg atttcagccg cgctgtactg gaggctgaag ttcagatgtg cggcgagttg
1561 cgtgactacc tacgggtaac agtttcttta tggcagggtg aaacgcaggt cgccagcggc
1621 accgcgcctt tcggcggtga aattatcgat gagcgtggtg gttatgccga tcgcgtcaca
1681 ctacgtctga acgtcgaaaa cccgaaactg tggagcgccg aaatcccgaa tctctatcgt
1741 gcggtggttg aactgcacac cgccgacggc acgctgattg aagcagaagc ctgcgatgtc
1801 ggtttccgcg aggtgcggat tgaaaatggt ctgctgctgc tgaacggcaa gccgttgctg
1861 attcgaggcg ttaaccgtca cgagcatcat cctctgcatg gtcaggtcat ggatgagcag
1921 acgatggtgc aggatatcct gctgatgaag cagaacaact ttaacgccgt gcgctgttcg
1981 cattatccga accatccgct gtggtacacg ctgtgcgacc gctacggcct gtatgtggtg
2041 gatgaagcca atattgaaac ccacggcatg gtgccaatga atcgtctgac cgatgatccg
2101 cgctggctac cggcgatgag cgaacgcgta acgcgaatgg tgcagcgcga tcgtaatcac
2161 ccgagtgtga tcatctggtc gctggggaat gaatcaggcc acggcgctaa tcacgacgcg
2221 ctgtatcgct ggatcaaatc tgtcgatcct tcccgcccgg tgcagtatga aggcggcgga
2281 gccgacacca cggccaccga tattatttgc ccgatgtacg cgcgcgtgga tgaagaccag
2341 cccttcccgg ctgtgccgaa atggtccatc aaaaaatggc tttcgctacc tggagagacg
2401 cgcccgctga tcctttgcga atacgcccac gcgatgggta acagtcttgg cggtttcgct
2461 aaatactggc aggcgtttcg tcagtatccc cgtttacagg gcggcttcgt ctgggactgg
2521 gtggatcagt cgctgattaa atatgatgaa aacggcaacc cgtggtcggc ttacggcggt
2581 gattttggcg atacgccgaa cgatcgccag ttctgtatga acggtctggt ctttgccgac
2641 cgcacgccgc atccagcgct gacggaagca aaacaccagc agcagttttt ccagttccgt
2701 ttatccgggc aaaccatcga agtgaccagc gaatacctgt tccgtcatag cgataacgag
2761 ctcctgcact ggatggtggc gctggatggt aagccgctgg caagcggtga agtgcctctg
2821 gatgtcgctc cacaaggtaa acagttgatt gaactgcctg aactaccgca gccggagagc
2881 gccgggcaac tctggctcac agtacgcgta gtgcaaccga acgcgaccgc atggtcagaa
2941 gccgggcaca tcagcgcctg gcagcagtgg cgtctggcgg aaaacctcag tgtgacgctc
3001 cccgccgcgt cccacgccat cccgcatctg accaccagcg aaatggattt ttgcatcgag
3061 ctgggtaata agcgttggca atttaaccgc cagtcaggct ttctttcaca gatgtggatt
3121 ggcgataaaa aacaactgct gacgccgctg cgcgatcagt tcacccgtgc accgctggat
3181 aacgacattg gcgtaagtga agcgacccgc attgacccta acgcctgggt cgaacgctgg
3241 aaggcggcgg gccattacca ggccgaagca gcgttgttgc agtgcacggc agatacactt
3301 gctgatgcgg tgctgattac gaccgctcac gcgtggcagc atcaggggaa aaccttattt
3361 atcagccgga aaacctaccg gattgatggt agtggtcaaa tggcgattac cgttgatgtt
3421 gaagtggcga gcgatacacc gcatccggcg cggattggcc tgaactgcca gctggcgcag
3481 gtagcagagc gggtaaactg gctcggatta gggccgcaag aaaactatcc cgaccgcctt
3541 actgccgcct gttttgaccg ctgggatctg ccattgtcag acatgtatac cccgtacgtc
3601 ttcccgagcg aaaacggtct gcgctgcggg acgcgcgaat tgaattatgg cccacaccag
3661 tggcgcggcg acttccagtt caacatcagc cgctacagtc aacagcaact gatggaaacc
3721 agccatcgcc atctgctgca cgcggaagaa ggcacatggc tgaatatcga cggtttccat
3781 atggggattg gtggcgacga ctcctggagc ccgtcagtat cggcggaatt ccagctgagc
3841 gccggtcgct accattacca gttggtctgg tgtcaaaaat aagcggccgc ggggatccag
3901 acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat
3961 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata
4021 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg
4081 aggttttttc ggatcctcta gagtcgatct gcaggcatgc tagcttggcg taatcatggt
4141 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
4201 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
4261 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
4321 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
4381 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
4441 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
4501 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
4561 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
4621 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
4681 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
4741 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
4801 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
4861 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
4921 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
4981 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
5041 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
5101 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
5161 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
5221 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
5281 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
5341 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
5401 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
5461 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
5521 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
5581 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
5641 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
5701 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
5761 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
5821 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
5881 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
5941 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
6001 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
6061 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
6121 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
6181 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
6241 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag
6301 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
6361 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
6421 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
6481 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
6541 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
6601 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
6661 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
6721 tttcccagtc acgacgttgt aaaacgacgg ccagt
//