LOCUS Exported 6013 bp ds-DNA circular SYN 27-SEP-2017
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6013)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Sep 27, 2017 from MiaoLingPlasmid
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..6013
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_feature 9..178
/label=mut p53 binding sites
CDS 480..2132
/codon_start=1
/gene="luc"
/product="firefly luciferase"
/label=luciferase
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
intron 2263..2328
/label=small t intron
/note="SV40 (simian virus 40) small t antigen intron"
CDS 2458..2478
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
polyA_signal 2903..3037
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(3580..4168)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(4339..5199)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(5200..5304)
/gene="bla"
/label=AmpR promoter
rep_origin complement(5330..5785)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5950..5968
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
1 agcttgatcc ttaatggact ttaatggcct tatggacttt aatggcctta atggacttta
61 atggccttaa tggactttaa tggccttaat ggactttaat ggccttaatg gactttaatg
121 gccttaatgg actttaatgg ccttaatgga ctttaatggc cttaatggac tttaatggcc
181 atcgaattcg atctgttttt tttagtatta agcagaggcc gggggcccct ggcctccgct
241 tactctggag aaaaagaaga gaggcattcc agaggcaact tgtcaaaaca ggactggcgc
301 cttggaggcg ctgtggggcc acccaaattg atataattaa gccccaaccg cctcttcccg
361 cctcatttca gcctcaccac cctcgacaag cttatcgata ccgtcggggg atccactagt
421 tctagagcgg ccgccaccgc ggtggagctc gagcttggca ttccggtact gttggtaaaa
481 tggaagacgc caaaaacata aagaaaggcc cggcgccatt ctatcctcta gaggatggaa
541 ccgctggaga gcaactgcat aaggctatga agagatacgc cctggttcct ggaacaattg
601 cttttacaga tgcacatatc gaggtgaaca tcacgtacgc ggaatacttc gaaatgtccg
661 ttcggttggc agaagctatg aaacgatatg ggctgaatac aaatcacaga atcgtcgtat
721 gcagtgaaaa ctctcttcaa ttctttatgc cggtgttggg cgcgttattt atcggagttg
781 cagttgcgcc cgcgaacgac atttataatg aacgtgaatt gctcaacagt atgaacattt
841 cgcagcctac cgtagtgttt gtttccaaaa aggggttgca aaaaattttg aacgtgcaaa
901 aaaaattacc aataatccag aaaattatta tcatggattc taaaacggat taccagggat
961 ttcagtcgat gtacacgttc gtcacatctc atctacctcc cggttttaat gaatacgatt
1021 ttgtaccaga gtcctttgat cgtgacaaaa caattgcact gataatgaat tcctctggat
1081 ctactgggtt acctaagggt gtggcccttc cgcatagaac tgcctgcgtc agattctcgc
1141 atgccagaga tcctattttt ggcaatcaaa tcattccgga tactgcgatt ttaagtgttg
1201 ttccattcca tcacggtttt ggaatgttta ctacactcgg atatttgata tgtggatttc
1261 gagtcgtctt aatgtataga tttgaagaag agctgttttt acgatccctt caggattaca
1321 aaattcaaag tgcgttgcta gtaccaaccc tattttcatt cttcgccaaa agcactctga
1381 ttgacaaata cgatttatct aatttacacg aaattgcttc tgggggcgca cctctttcga
1441 aagaagtcgg ggaagcggtt gcaaaacgct tccatcttcc agggatacga caaggatatg
1501 ggctcactga gactacatca gctattctga ttacacccga gggggatgat aaaccgggcg
1561 cggtcggtaa agttgttcca ttttttgaag cgaaggttgt ggatctggat accgggaaaa
1621 cgctgggcgt taatcagaga ggcgaattat gtgtcagagg acctatgatt atgtccggtt
1681 atgtaaacaa tccggaagcg accaacgcct tgattgacaa ggatggatgg ctacattctg
1741 gagacatagc ttactgggac gaagacgaac acttcttcat agttgaccgc ttgaagtctt
1801 taattaaata caaaggatat caggtggccc ccgctgaatt ggaatcgata ttgttacaac
1861 accccaacat cttcgacgcg ggcgtggcag gtcttcccga cgatgacgcc ggtgaacttc
1921 ccgccgccgt tgttgttttg gagcacggaa agacgatgac ggaaaaagag atcgtggatt
1981 acgtcgccag tcaagtaaca accgcgaaaa agttgcgcgg aggagttgtg tttgtggacg
2041 aagtaccgaa aggtcttacc ggaaaactcg acgcaagaaa aatcagagag atcctcataa
2101 aggccaagaa gggcggaaag tccaaattgt aaaatgtaac tgtattcagc gatgacgaaa
2161 ttcttagcta ttgtaatact ctagaggatc tttgtgaagg aaccttactt ctgtggtgtg
2221 acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta
2281 agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat
2341 ggaactgatg aatgggagca gtggtggaat gcctttaatg aggaaaacct gttttgctca
2401 gaagaaatgc catctagtga tgatgaggct actgctgact ctcaacattc tactcctcca
2461 aaaaagaaga gaaaggtaga agaccccaag gactttcctt cagaattgct aagttttttg
2521 agtcatgctg tgtttagtaa tagaactctt gcttgctttg ctatttacac cacaaaggaa
2581 aaagctgcac tgctatacaa gaaaattatg gaaaaatatt ctgtaacctt tataagtagg
2641 cataacagtt ataatcataa catactgttt tttcttactc cacacaggca tagagtgtct
2701 gctattaata actatgctca aaaattgtgt acctttagct ttttaatttg taaaggggtt
2761 aataaggaat atttgatgta tagtgccttg actagagatc ataatcagcc ataccacatt
2821 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
2881 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
2941 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
3001 gtccaaactc atcaatgtat cttatcatgt ctggatccac tagttctaga gcggccgcca
3061 ccgcggtgga gctccagctt ttgttccctt tagtgagggt taatttcgag cttggcgtaa
3121 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
3181 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
3241 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
3301 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcatt aatgaatcgg
3361 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
3421 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
3481 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
3541 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
3601 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
3661 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
3721 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
3781 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
3841 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
3901 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
3961 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
4021 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
4081 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
4141 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
4201 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
4261 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
4321 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
4381 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
4441 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
4501 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
4561 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
4621 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
4681 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
4741 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
4801 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
4861 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
4921 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
4981 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
5041 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
5101 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
5161 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
5221 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
5281 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccaccta aattgtaagc
5341 gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa
5401 taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt
5461 gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg
5521 cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt
5581 ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga
5641 gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg
5701 ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg
5761 cttaatgcgc cgctacaggg cgcgtcccat tcgccattca ggctgcgcaa ctgttgggaa
5821 gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca
5881 aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc
5941 agtgaattgt aatacgactc actatagggc gaattgggta ccgggccccc cctcgaggtc
6001 gacggtatcg ata
//