LOCUS Exported File 5064 bp ds-DNA circular SYN 23-3-2015
DEFINITION .
ACCESSION .
VERSION .
KEYWORDS Untitled
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5064)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported 2015-3-23 from MiaoLingplasmid
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..5064
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS 88..1740
/codon_start=1
/gene="luc+"
/product="firefly luciferase"
/EC_number="
"
/note="luciferase"
/note="enhanced?luc+ version of the luciferase gene"
/protein_id="
"
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
polyA_signal 1781..1902
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin complement(2567..3155)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(3326..4186)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(4187..4291)
/gene="bla"
/note="AmpR promoter"
rep_origin 4318..4773
/direction=RIGHT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
polyA_signal 4904..4952
/note="synthetic polyadenylation signal"
misc_feature 4966..5057
/note="pause site"
/note="RNA polymerase II transcriptional pause signal from
the human?alpha-2 globin gene"
ORIGIN
1 ggtaccgagc tcttacgcgt gctagcccgg gctcgagatc tgcgatctaa gtaagcttgg
61 cattccggta ctgttggtaa agccaccatg gaagacgcca aaaacataaa gaaaggcccg
121 gcgccattct atccgctgga agatggaacc gctggagagc aactgcataa ggctatgaag
181 agatacgccc tggttcctgg aacaattgct tttacagatg cacatatcga ggtggacatc
241 acttacgctg agtacttcga aatgtccgtt cggttggcag aagctatgaa acgatatggg
301 ctgaatacaa atcacagaat cgtcgtatgc agtgaaaact ctcttcaatt ctttatgccg
361 gtgttgggcg cgttatttat cggagttgca gttgcgcccg cgaacgacat ttataatgaa
421 cgtgaattgc tcaacagtat gggcatttcg cagcctaccg tggtgttcgt ttccaaaaag
481 gggttgcaaa aaattttgaa cgtgcaaaaa aagctcccaa tcatccaaaa aattattatc
541 atggattcta aaacggatta ccagggattt cagtcgatgt acacgttcgt cacatctcat
601 ctacctcccg gttttaatga atacgatttt gtgccagagt ccttcgatag ggacaagaca
661 attgcactga tcatgaactc ctctggatct actggtctgc ctaaaggtgt cgctctgcct
721 catagaactg cctgcgtgag attctcgcat gccagagatc ctatttttgg caatcaaatc
781 attccggata ctgcgatttt aagtgttgtt ccattccatc acggttttgg aatgtttact
841 acactcggat atttgatatg tggatttcga gtcgtcttaa tgtatagatt tgaagaagag
901 ctgtttctga ggagccttca ggattacaag attcaaagtg cgctgctggt gccaacccta
961 ttctccttct tcgccaaaag cactctgatt gacaaatacg atttatctaa tttacacgaa
1021 attgcttctg gtggcgctcc cctctctaag gaagtcgggg aagcggttgc caagaggttc
1081 catctgccag gtatcaggca aggatatggg ctcactgaga ctacatcagc tattctgatt
1141 acacccgagg gggatgataa accgggcgcg gtcggtaaag ttgttccatt ttttgaagcg
1201 aaggttgtgg atctggatac cgggaaaacg ctgggcgtta atcaaagagg cgaactgtgt
1261 gtgagaggtc ctatgattat gtccggttat gtaaacaatc cggaagcgac caacgccttg
1321 attgacaagg atggatggct acattctgga gacatagctt actgggacga agacgaacac
1381 ttcttcatcg ttgaccgcct gaagtctctg attaagtaca aaggctatca ggtggctccc
1441 gctgaattgg aatccatctt gctccaacac cccaacatct tcgacgcagg tgtcgcaggt
1501 cttcccgacg atgacgccgg tgaacttccc gccgccgttg ttgttttgga gcacggaaag
1561 acgatgacgg aaaaagagat cgtggattac gtcgccagtc aagtaacaac cgcgaaaaag
1621 ttgcgcggag gagttgtgtt tgtggacgaa gtaccgaaag gtcttaccgg aaaactcgac
1681 gcaagaaaaa tcagagagat cctcataaag gccaagaagg gcggaaagat cgccgtgtaa
1741 ttctagagtc ggggcggccg gccgcttcga gcagacatga taagatacat tgatgagttt
1801 ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct
1861 attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt
1921 cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc
1981 tacaaatgtg gtaaaatcga taaggatctg aacgatggag cggagaatgg gcggaactgg
2041 gcggagttag gggcgggatg ggcggagtta ggggcgggac tatggttgct gactaattga
2101 gatgcatgct ttgcatactt ctgcctgctg gggagcctgg ggactttcca cacctggttg
2161 ctgactaatt gagatgcatg ctttgcatac ttctgcctgc tggggagcct ggggactttc
2221 cacaccctaa ctgacacaca ttccacagcg gatccgtcga ccgatgccct tgagagcctt
2281 caacccagtc agctccttcc ggtgggcgcg gggcatgact atcgtcgccg cacttatgac
2341 tgtcttcttt atcatgcaac tcgtaggaca ggtgccggca gcgctcttcc gcttcctcgc
2401 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
2461 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
2521 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
2581 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
2641 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
2701 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
2761 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
2821 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
2881 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
2941 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
3001 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
3061 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
3121 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
3181 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
3241 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
3301 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
3361 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
3421 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
3481 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
3541 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
3601 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
3661 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
3721 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
3781 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
3841 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
3901 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
3961 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
4021 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
4081 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
4141 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
4201 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
4261 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
4321 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
4381 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
4441 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
4501 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
4561 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
4621 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
4681 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
4741 cgaattttaa caaaatatta acgcttacaa tttgccattc gccattcagg ctgcgcaact
4801 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagcccaag ctaccatgat
4861 aagtaagtaa tattaaggta cgggaggtac ttggagcggc cgcaataaaa tatctttatt
4921 ttcattacat ctgtgtgttg gttttttgtg tgaatcgata gtactaacat acgctctcca
4981 tcaaaacaaa acgaaacaaa acaaactagc aaaataggct gtccccagtg caagtgcagg
5041 tgccagaaca tttctctatc gata
//