LOCUS Exported 4787 bp ds-DNA circular SYN 17-AUG-2017
DEFINITION synthetic circular DNA
KEYWORDS pKillerRed-mem.dna
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4787)
FEATURES Location/Qualifiers
source 1..4787
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 61..364
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 365..568
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
misc_feature 591..671
/label=MCS
/note="multiple cloning site"
misc_feature 679..738
/label=mem
misc_feature 739..1452
/label=KillerRed
polyA_signal 1575..1696
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(1703..2158)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2185..2289
/gene="bla"
/label=AmpR promoter
promoter 2291..2648
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 2499..2634
/label=SV40 ori
/note="SV40 origin of replication"
CDS 2683..3477
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 3709..3756
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 4085..4673
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
601 ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc gcgggcccgg
661 gatccaccgg tcgccaccat gctgtgctgt atgagaagaa ccaaacaggt tgaaaagaat
721 gatgaggacc aaaagatctc cgagggcggc cccgccctgt tccagagcga catgaccttc
781 aaaatcttca tcgacggcga ggtgaacggc cagaagttca ccatcgtggc cgacggcagc
841 agcaagttcc cccacggcga cttcaacgtg cacgccgtgt gcgagaccgg caagctgccc
901 atgagctgga agcccatctg ccacctgatc cagtacggcg agcccttctt cgcccgctac
961 cccgacggca tcagccattt cgcccaggag tgcttccccg agggcctgag catcgaccgc
1021 accgtgcgct tcgagaacga cggcaccatg accagccacc acacctacga gctggacgac
1081 acctgcgtgg tgagccgcat caccgtgaac tgcgacggct tccagcccga cggccccatc
1141 atgcgcgacc agctggtgga catcctgccc aacgagaccc acatgttccc ccacggcccc
1201 aacgccgtgc gccagctggc cttcatcggc ttcaccaccg ccgacggcgg cctgatgatg
1261 ggccacttcg acagcaagat gaccttcaac ggcagccgcg ccatcgagat ccccggccca
1321 cacttcgtga ccatcatcac caagcagatg agggacacca gcgacaagcg cgaccacgtg
1381 tgccagcgcg aggtggccta cgcccacagc gtgccccgca tcaccagcgc catcggtagc
1441 gacgaggatt aaagcggccg cgactctaga tcataatcag ccataccaca tttgtagagg
1501 ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg
1561 caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
1621 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
1681 tcatcaatgt atcttaaggc gtaaattgta agcgttaata ttttgttaaa attcgcgtta
1741 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat
1801 aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca
1861 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc
1921 ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta
1981 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg
2041 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg
2101 gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtca
2161 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
2221 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
2281 aggaagagtc ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa
2341 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
2401 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
2461 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
2521 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
2581 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
2641 tttgcaaaga tcgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga
2701 ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa
2761 cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt
2821 ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg
2881 ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa
2941 gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac
3001 cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt
3061 gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact
3121 cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg
3181 ccagccgaac tgttcgccag gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg
3241 acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc
3301 atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt
3361 gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc
3421 gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgagcg
3481 ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca cgagatttcg
3541 attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct
3601 ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccaccct agggggaggc
3661 taactgaaac acggaaggag acaataccgg aaggaacccg cgctatgacg gcaataaaaa
3721 gacagaataa aacgcacggt gttgggtcgt ttgttcataa acgcggggtt cggtcccagg
3781 gctggcactc tgtcgatacc ccaccgagac cccattgggg ccaatacgcc cgcgtttctt
3841 ccttttcccc accccacccc ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg
3901 gggcggcagg ccctgccata gcctcaggtt actcatatat actttagatt gatttaaaac
3961 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
4021 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
4081 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
4141 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
4201 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
4261 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
4321 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
4381 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
4441 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
4501 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
4561 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
4621 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
4681 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
4741 ctgcgttatc ccctgattct gtggataacc gtattaccgc catgcat
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