LOCUS Exported 5539 bp ds-DNA circular SYN 13-七月-2017
KEYWORDS TopFlash
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5539)
TITLE Direct Submission
JOURNAL Exported 2017年7月13日 from MiaoLingPlasmid
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..5539
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 600..662
/note="Mini-TK promoter"
/note="minimal herpes simplex virus (HSV) thymidine kinase
promoter"
CDS 691..2343
/codon_start=1
/gene="luc"
/product="firefly luciferase"
/EC_number="
/note="luciferase"
/experiment="
/protein_id="
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
intron 2502..2567
/note="small t intron"
/note="simian virus 40 (SV40) small t antigen intron"
CDS 2697..2717
/codon_start=1
/locus_tag="
"
/product="nuclear localization signal of SV40 large T
antigen"
/note="SV40 NLS"
/note="
/protein_id="
/translation="PKKKRKV"
polyA_signal 3142..3276
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin complement(3720..4308)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(4479..5339)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(5340..5444)
/gene="bla"
/note="AmpR promoter"
ORIGIN
1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gttaagatca aagggggtaa
421 gakcaaaggg ggtaaaatca aagggggccc cctttgatct tacccccttt gatcttaccc
481 cctttgatct tactgcatgc ctgcaggtcg actctagagg atccggcccc gcccagcgtc
541 ttgtcattgg cgaattcgaa cacgcagatg cagtcggggc ggcgcggtcc gaggtccact
601 tcgcatatta aggtgacgcg tgtggcctcg aacaccgagc gaccctgcag cgacccgctt
661 aacagcgtca acagcgtgcc gcagatctcc atggaagacg ccaaaaacat aaagaaaggc
721 ccggcgccat tctatcctct agaggatgga accgctggag agcaactgca taaggctatg
781 aagagatacg ccctggttcc tggaacaatt gcttttacag atgcacatat cgaggtgaac
841 atcacgtacg cggaatactt cgaaatgtcc gttcggttgg cagaagctat gaaacgatat
901 gggctgaata caaatcacag aatcgtcgta tgcagtgaaa actctcttca attctttatg
961 ccggtgttgg gcgcgttatt tatcggagtt gcagttgcgc ccgcgaacga catttataat
1021 gaacgtgaat tgctcaacag tatgaacatt tcgcagccta ccgtagtgtt tgtttccaaa
1081 aaggggttgc aaaaaatttt gaacgtgcaa aaaaaattac caataatcca gaaaattatt
1141 atcatggatt ctaaaacgga ttaccaggga tttcagtcga tgtacacgtt cgtcacatct
1201 catctacctc ccggttttaa tgaatacgat tttgtaccag agtcctttga tcgtgacaaa
1261 acaattgcac tgataatgaa ttcctctgga tctactgggt tacctaaggg tgtggccctt
1321 ccgcatagaa ctgcctgcgt cagattctcg catgccagag atcctatttt tggcaatcaa
1381 atcattccgg atactgcgat tttaagtgtt gttccattcc atcacggttt tggaatgttt
1441 actacactcg gatatttgat atgtggattt cgagtcgtct taatgtatag atttgaagaa
1501 gagctgtttt tacgatccct tcaggattac aaaattcaaa gtgcgttgct agtaccaacc
1561 ctattttcat tcttcgccaa aagcactctg attgacaaat acgatttatc taatttacac
1621 gaaattgctt ctgggggcgc acctctttcg aaagaagtcg gggaagcggt tgcaaaacgc
1681 ttccatcttc cagggatacg acaaggatat gggctcactg agactacatc agctattctg
1741 attacacccg agggggatga taaaccgggc gcggtcggta aagttgttcc attttttgaa
1801 gcgaaggttg tggatctgga taccgggaaa acgctgggcg ttaatcagag aggcgaatta
1861 tgtgtcagag gacctatgat tatgtccggt tatgtaaaca atccggaagc gaccaacgcc
1921 ttgattgaca aggatggatg gctacattct ggagacatag cttactggga cgaagacgaa
1981 cacttcttca tagttgaccg cttgaagtct ttaattaaat acaaaggata tcaggtggcc
2041 cccgctgaat tggaatcgat attgttacaa caccccaaca tcttcgacgc gggcgtggca
2101 ggtcttcccg acgatgacgc cggtgaactt cccgccgccg ttgttgtttt ggagcacgga
2161 aagacgatga cggaaaaaga gatcgtggat tacgtcgcca gtcaagtaac aaccgcgaaa
2221 aagttgcgcg gaggagttgt gtttgtggac gaagtaccga aaggtcttac cggaaaactc
2281 gacgcaagaa aaatcagaga gatcctcata aaggccaaga agggcggaaa gtccaaattg
2341 taaaatgtaa ctgtattcag cgatgacgaa attcttagct attgtaatga attcgagctc
2401 caccgcggtg gcggccgctc tagaggatct ttgtgaagga accttacttc tgtggtgtga
2461 cataattgga caaactacct acagagattt aaagctctaa ggtaaatata aaatttttaa
2521 gtgtataatg tgttaaacta ctgattctaa ttgtttgtgt attttagatt ccaacctatg
2581 gaactgatga atgggagcag tggtggaatg cctttaatga ggaaaacctg ttttgctcag
2641 aagaaatgcc atctagtgat gatgaggcta ctgctgactc tcaacattct actcctccaa
2701 aaaagaagag aaaggtagaa gaccccaagg actttccttc agaattgcta agttttttga
2761 gtcatgctgt gtttagtaat agaactcttg cttgctttgc tatttacacc acaaaggaaa
2821 aagctgcact gctatacaag aaaattatgg aaaaatattc tgtaaccttt ataagtaggc
2881 ataacagtta taatcataac atactgtttt ttcttactcc acacaggcat agagtgtctg
2941 ctattaataa ctatgctcaa aaattgtgta cctttagctt tttaatttgt aaaggggtta
3001 ataaggaata tttgatgtat agtgccttga ctagagatca taatcagcca taccacattt
3061 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa
3121 atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc
3181 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
3241 tccaaactca tcaatgtatc ttatcatgtc tggatctcga gggggggccc ggtaccgagc
3301 tcgaattcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
3361 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
3421 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
3481 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
3541 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
3601 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
3661 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
3721 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
3781 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
3841 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
3901 gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
3961 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
4021 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
4081 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
4141 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
4201 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
4261 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
4321 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
4381 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
4441 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
4501 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
4561 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
4621 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
4681 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
4741 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
4801 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
4861 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
4921 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
4981 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
5041 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
5101 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
5161 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
5221 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
5281 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
5341 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
5401 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
5461 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
5521 atcacgaggc cctttcgtc
//