LOCUS Exported 4281 bp ds-DNA circular SYN 26-JUL-2013
DEFINITION Co-transfection vector for expressing Renilla luciferase under
control of the strong CMV promoter.
ACCESSION AY738231
VERSION .
KEYWORDS pGL4.75[hRluc CMV]
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4281)
AUTHORS Promega
TITLE Direct Submission
JOURNAL Exported Sunday, September 4, 2016 from SnapGene Viewer 3.1.4
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..4281
/organism="synthetic DNA construct"
/lab_host="Mammalian Cells"
/mol_type="other DNA"
enhancer 227..530
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 531..734
/note="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
CDS 859..1794
/codon_start=1
/product="Renilla luciferase"
/note="hRluc"
/note="human codon-optimized"
/translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
polyA_signal 1835..1956
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin complement(2375..2963)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(3163..4023)
/codon_start=1
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
polyA_signal 4128..4176
/note="synthetic polyadenylation signal"
misc_feature 4190..4281
/note="pause site"
/note="RNA polymerase II transcriptional pause signal from
the human alpha-2 globin gene"
ORIGIN
1 ggcctaactg gcctcaatat tggccattag ccatattatt cattggttat atagcataaa
61 tcaatattgg ctattggcca ttgcatacgt tgtatctata tcataatatg tacatttata
121 ttggctcatg tccaatatga ccgccatgtt ggcattgatt attgactagt tattaatagt
181 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
241 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
301 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
361 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt ccgcccccta
421 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttacggg
481 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt
541 tttggcagta caccaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
601 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
661 gtcgtaataa ccccgccccg ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
721 atataagcag agctcgttta gtgaaccgtc agatcactag aagctttatt gcggtagttt
781 atcacagtta aattgctaac gcagtcagtg ggcctcggcg gccaagcttg gcaatccggt
841 actgttggta aagccaccat ggcttccaag gtgtacgacc ccgagcaacg caaacgcatg
901 atcactgggc ctcagtggtg ggctcgctgc aagcaaatga acgtgctgga ctccttcatc
961 aactactatg attccgagaa gcacgccgag aacgccgtga tttttctgca tggtaacgct
1021 gcctccagct acctgtggag gcacgtcgtg cctcacatcg agcccgtggc tagatgcatc
1081 atccctgatc tgatcggaat gggtaagtcc ggcaagagcg ggaatggctc atatcgcctc
1141 ctggatcact acaagtacct caccgcttgg ttcgagctgc tgaaccttcc aaagaaaatc
1201 atctttgtgg gccacgactg gggggcttgt ctggcctttc actactccta cgagcaccaa
1261 gacaagatca aggccatcgt ccatgctgag agtgtcgtgg acgtgatcga gtcctgggac
1321 gagtggcctg acatcgagga ggatatcgcc ctgatcaaga gcgaagaggg cgagaaaatg
1381 gtgcttgaga ataacttctt cgtcgagacc atgctcccaa gcaagatcat gcggaaactg
1441 gagcctgagg agttcgctgc ctacctggag ccattcaagg agaagggcga ggttagacgg
1501 cctaccctct cctggcctcg cgagatccct ctcgttaagg gaggcaagcc cgacgtcgtc
1561 cagattgtcc gcaactacaa cgcctacctt cgggccagcg acgatctgcc taagatgttc
1621 atcgagtccg accctgggtt cttttccaac gctattgtcg agggagctaa gaagttccct
1681 aacaccgagt tcgtgaaggt gaagggcctc cacttcagcc aggaggacgc tccagatgaa
1741 atgggtaagt acatcaagag cttcgtggag cgcgtgctga agaacgagca gtaattctag
1801 agtcggggcg gccggccgct tcgagcagac atgataagat acattgatga gtttggacaa
1861 accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct
1921 ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt
1981 atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa
2041 tgtggtaaaa tcgataagga tccgtcgacc gatgcccttg agagccttca acccagtcag
2101 ctccttccgg tgggcgcggg gcatgactat cgtcgccgca cttatgactg tcttctttat
2161 catgcaactc gtaggacagg tgccggcagc gctcttccgc ttcctcgctc actgactcgc
2221 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
2281 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
2341 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
2401 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
2461 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
2521 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
2581 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
2641 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
2701 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
2761 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
2821 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
2881 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
2941 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
3001 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
3061 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
3121 cttggtctga cagcggccgc aaatgctaaa ccactgcagt ggttaccagt gcttgatcag
3181 tgaggcaccg atctcagcga tctgcctatt tcgttcgtcc atagtggcct gactccccgt
3241 cgtgtagatc actacgattc gtgagggctt accatcaggc cccagcgcag caatgatgcc
3301 gcgagagccg cgttcaccgg cccccgattt gtcagcaatg aaccagccag cagggagggc
3361 cgagcgaaga agtggtcctg ctactttgtc cgcctccatc cagtctatga gctgctgtcg
3421 tgatgctaga gtaagaagtt cgccagtgag tagtttccga agagttgtgg ccattgctac
3481 tggcatcgtg gtatcacgct cgtcgttcgg tatggcttcg ttcaactctg gttcccagcg
3541 gtcaagccgg gtcacatgat cacccatatt atgaagaaat gcagtcagct ccttagggcc
3601 tccgatcgtt gtcagaagta agttggccgc ggtgttgtcg ctcatggtaa tggcagcact
3661 acacaattct cttaccgtca tgccatccgt aagatgcttt tccgtgaccg gcgagtactc
3721 aaccaagtcg ttttgtgagt agtgtatacg gcgaccaagc tgctcttgcc cggcgtctat
3781 acgggacaac accgcgccac atagcagtac tttgaaagtg ctcatcatcg ggaatcgttc
3841 ttcggggcgg aaagactcaa ggatcttgcc gctattgaga tccagttcga tatagcccac
3901 tcttgcaccc agttgatctt cagcatcttt tactttcacc agcgtttcgg ggtgtgcaaa
3961 aacaggcaag caaaatgccg caaagaaggg aatgagtgcg acacgaaaat gttggatgct
4021 catactcgtc ctttttcaat attattgaag catttatcag ggttactagt acgtctctca
4081 aggataagta agtaatatta aggtacggga ggtattggac aggccgcaat aaaatatctt
4141 tattttcatt acatctgtgt gttggttttt tgtgtgaatc gatagtacta acatacgctc
4201 tccatcaaaa caaaacgaaa caaaacaaac tagcaaaata ggctgtcccc agtgcaagtg
4261 caggtgccag aacatttctc t
//