LOCUS Exported File 4045 bp ds-DNA circular SYN 01-4-2015
DEFINITION .
ACCESSION .
VERSION .
KEYWORDS pRL-TK
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4045)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported 2015-4-1 from MLCC
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..4045
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 8..759
/note="HSV TK promoter"
/note="herpes simplex virus thymidine kinase promoter"
intron 829..961
/note="chimeric intron"
/note="chimera between introns from human?beta-globin and
immunoglobulin heavy chain genes"
promoter 1006..1024
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
polyA_signal 2000..2121
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
promoter 2254..2358
/gene="bla"
/note="AmpR promoter"
CDS 2359..3219
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 3390..3978
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 agatctaaat gagtcttcgg acctcgcggg ggccgcttaa gcggtggtta gggtttgtct
61 gacgcggggg gagggggaag gaacgaaaca ctctcattcg gaggcggctc ggggtttggt
121 cttggtggcc acgggcacgc agaagagcgc cgcgatcctc ttaagcaccc ccccgccctc
181 cgtggaggcg ggggtttggt cggcgggtgg taactggcgg gccgctgact cgggcgggtc
241 gcgcgcccca gagtgtgacc ttttcggtct gctcgcagac ccccgggcgg cgccgccgcg
301 gcggcgacgg gctcgctggg tcctaggctc catggggacc gtatacgtgg acaggctctg
361 gagcatccgc acgactgcgg tgatattacc ggagaccttc tgcgggacga gccgggtcac
421 gcggctgacg cggagcgtcc gttgggcgac aaacaccagg acggggcaca ggtacactat
481 cttgtcaccc ggaggcgcga gggactgcag gagcttcagg gagtggcgca gctgcttcat
541 ccccgtggcc cgttgctcgc gtttgctggc ggtgtccccg gaagaaatat atttgcatgt
601 ctttagttct atgatgacac aaaccccgcc cagcgtcttg tcattggcga attcgaacac
661 gcagatgcag tcggggcggc gcggtcccag gtccacttcg catattaagg tgacgcgtgt
721 ggcctcgaac accgagcgac cctgcagcga cccgcttaaa agcttgattc ttctgacaca
781 acagtctcga acttaagctg cagaagttgg tcgtgaggca ctgggcaggt aagtatcaag
841 gttacaagac aggtttaagg agaccaatag aaactgggct tgtcgagaca gagaagactc
901 ttgcgtttct gataggcacc tattggtctt actgacatcc actttgcctt tctctccaca
961 ggtgtccact cccagttcaa ttacagctct taaggctaga gtacttaata cgactcacta
1021 taggctagcc accatgactt cgaaagttta tgatccagaa caaaggaaac ggatgataac
1081 tggtccgcag tggtgggcca gatgtaaaca aatgaatgtt cttgattcat ttattaatta
1141 ttatgattca gaaaaacatg cagaaaatgc tgttattttt ttacatggta acgcggcctc
1201 ttcttattta tggcgacatg ttgtgccaca tattgagcca gtagcgcggt gtattatacc
1261 agaccttatt ggtatgggca aatcaggcaa atctggtaat ggttcttata ggttacttga
1321 tcattacaaa tatcttactg catggtttga acttcttaat ttaccaaaga agatcatttt
1381 tgtcggccat gattggggtg cttgtttggc atttcattat agctatgagc atcaagataa
1441 gatcaaagca atagttcacg ctgaaagtgt agtagatgtg attgaatcat gggatgaatg
1501 gcctgatatt gaagaagata ttgcgttgat caaatctgaa gaaggagaaa aaatggtttt
1561 ggagaataac ttcttcgtgg aaaccatgtt gccatcaaaa atcatgagaa agttagaacc
1621 agaagaattt gcagcatatc ttgaaccatt caaagagaaa ggtgaagttc gtcgtccaac
1681 attatcatgg cctcgtgaaa tcccgttagt aaaaggtggt aaacctgacg ttgtacaaat
1741 tgttaggaat tataatgctt atctacgtgc aagtgatgat ttaccaaaaa tgtttattga
1801 atcggaccca ggattctttt ccaatgctat tgttgaaggt gccaagaagt ttcctaatac
1861 tgaatttgtc aaagtaaaag gtcttcattt ttcgcaagaa gatgcacctg atgaaatggg
1921 aaaatatatc aaatcgttcg ttgagcgagt tctcaaaaat gaacaataat tctagagcgg
1981 ccgcttcgag cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
2041 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
2101 ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag
2161 ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg taaaatcgat
2221 aaggatccag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
2281 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
2341 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
2401 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
2461 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
2521 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
2581 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
2641 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
2701 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
2761 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
2821 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
2881 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
2941 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
3001 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
3061 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
3121 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
3181 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
3241 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
3301 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
3361 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
3421 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
3481 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
3541 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
3601 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
3661 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
3721 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
3781 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
3841 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
3901 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
3961 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
4021 tggccttttg ctcacatggc tcgac
//