LOCUS Exported File 3705 bp ds-DNA circular SYN 01-4-2015
DEFINITION .
ACCESSION .
VERSION .
KEYWORDS Untitled
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3705)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported 2015-4-1 from MLCC
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..3705
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 62..419
/note="SV40 promoter"
/note="SV40 enhancer and early promoter"
rep_origin 270..405
/note="SV40 ori"
/note="SV40 origin of replication"
intron 489..621
/note="chimeric intron"
/note="chimera between introns from human?beta-globin and
immunoglobulin heavy chain genes"
promoter 666..684
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
polyA_signal 1660..1781
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
promoter 1914..2018
/gene="bla"
/note="AmpR promoter"
CDS 2019..2879
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 3050..3638
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 agatctgcgc agcaccatgg cctgaaataa cctctgaaag aggaacttgg ttaggtacct
61 tctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
121 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
181 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
241 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
301 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
361 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
421 agcttgattc ttctgacaca acagtctcga acttaagctg cagaagttgg tcgtgaggca
481 ctgggcaggt aagtatcaag gttacaagac aggtttaagg agaccaatag aaactgggct
541 tgtcgagaca gagaagactc ttgcgtttct gataggcacc tattggtctt actgacatcc
601 actttgcctt tctctccaca ggtgtccact cccagttcaa ttacagctct taaggctaga
661 gtacttaata cgactcacta taggctagcc accatgactt cgaaagttta tgatccagaa
721 caaaggaaac ggatgataac tggtccgcag tggtgggcca gatgtaaaca aatgaatgtt
781 cttgattcat ttattaatta ttatgattca gaaaaacatg cagaaaatgc tgttattttt
841 ttacatggta acgcggcctc ttcttattta tggcgacatg ttgtgccaca tattgagcca
901 gtagcgcggt gtattatacc agaccttatt ggtatgggca aatcaggcaa atctggtaat
961 ggttcttata ggttacttga tcattacaaa tatcttactg catggtttga acttcttaat
1021 ttaccaaaga agatcatttt tgtcggccat gattggggtg cttgtttggc atttcattat
1081 agctatgagc atcaagataa gatcaaagca atagttcacg ctgaaagtgt agtagatgtg
1141 attgaatcat gggatgaatg gcctgatatt gaagaagata ttgcgttgat caaatctgaa
1201 gaaggagaaa aaatggtttt ggagaataac ttcttcgtgg aaaccatgtt gccatcaaaa
1261 atcatgagaa agttagaacc agaagaattt gcagcatatc ttgaaccatt caaagagaaa
1321 ggtgaagttc gtcgtccaac attatcatgg cctcgtgaaa tcccgttagt aaaaggtggt
1381 aaacctgacg ttgtacaaat tgttaggaat tataatgctt atctacgtgc aagtgatgat
1441 ttaccaaaaa tgtttattga atcggaccca ggattctttt ccaatgctat tgttgaaggt
1501 gccaagaagt ttcctaatac tgaatttgtc aaagtaaaag gtcttcattt ttcgcaagaa
1561 gatgcacctg atgaaatggg aaaatatatc aaatcgttcg ttgagcgagt tctcaaaaat
1621 gaacaataat tctagagcgg ccgcttcgag cagacatgat aagatacatt gatgagtttg
1681 gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta
1741 ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc
1801 attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag taaaacctct
1861 acaaatgtgg taaaatcgat aaggatccag gtggcacttt tcggggaaat gtgcgcggaa
1921 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
1981 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
2041 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
2101 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
2161 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
2221 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
2281 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
2341 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
2401 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
2461 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
2521 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
2581 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
2641 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
2701 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
2761 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
2821 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
2881 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
2941 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
3001 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
3061 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
3121 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
3181 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
3241 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
3301 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
3361 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
3421 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
3481 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
3541 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
3601 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
3661 tacggttcct ggccttttgc tggccttttg ctcacatggc tcgac
//