LOCUS Exported 6360 bp ds-DNA circular SYN 05-SEP-2016
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS Untitled 8
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6360)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Monday, September 5, 2016 from SnapGene Viewer 3.1.4
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..6360
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 138..517
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 518..729
/note="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
intron 890..1022
/note="chimeric intron"
/note="chimera between introns from human beta-globin and
immunoglobulin heavy chain genes"
promoter 1067..1085
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 1116..1556
/codon_start=1
/gene="Saccharomyces cerevisiae GAL4 (truncated)"
/product="DNA binding domain of the GAL4 transcriptional
activator"
/note="GAL4 DNA binding domain"
/translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
promoter complement(1640..1657)
/note="T3 promoter"
/note="promoter for bacteriophage T3 RNA polymerase"
polyA_signal 1675..1796
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin 1982..2437
/direction=RIGHT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2509..2866
/note="SV40 promoter"
/note="SV40 enhancer and early promoter"
rep_origin 2717..2852
/note="SV40 ori"
/note="SV40 origin of replication"
CDS 3208..4143
/codon_start=1
/product="luciferase from the anthozoan coelenterate
Renilla reniformis (sea pansy)"
/note="Rluc"
/translation="MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
polyA_signal 4201..4249
/note="synthetic polyadenylation signal"
promoter 4563..4667
/gene="bla"
/note="AmpR promoter"
CDS 4668..5528
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5699..6287
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactg
661 cgatcgcccg ccccgttgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
721 agcagagctc gtttagtgaa ccgtcagatc actagaagct ttattgcggt agtttatcac
781 agttaaattg ctaacgcagt cagtgcttct gacacaacag tctcgaactt aagctgcagt
841 gactctctta aggtagcctt gcagaagttg gtcgtgaggc actgggcagg taagtatcaa
901 ggttacaaga caggtttaag gagaccaata gaaactgggc ttgtcgagac agagaagact
961 cttgcgtttc tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac
1021 aggtgtccac tcccagttca attacagctc ttaaggctag agtacttaat acgactcact
1081 ataggctagc cagcttgaag caagcctcct gaaagatgaa gctactgtct tctatcgaac
1141 aagcatgcga tatttgccga cttaaaaagc tcaagtgctc caaagaaaaa ccgaagtgcg
1201 ccaagtgtct gaagaacaac tgggagtgtc gctactctcc caaaaccaaa aggtctccgc
1261 tgactagggc acatctgaca gaagtggaat caaggctaga aagactggaa cagctatttc
1321 tactgatttt tcctcgagaa gaccttgaca tgattttgaa aatggattct ttacaggata
1381 taaaagcatt gttaacagga ttatttgtac aagataatgt gaataaagat gccgtcacag
1441 atagattggc ttcagtggag actgatatgc ctctaacatt gagacagcat agaataagtg
1501 cgacatcatc atcggaagag agtagtaaca aaggtcaaag acagttgact gtatcgccgg
1561 aattcccggg gatccgtcga cttgacgcgt tgatatcatc tagagcggcc gcaggtacct
1621 gaataactaa ggccgcttcc ctttagtgag ggttaatgct tcgagcagac atgataagat
1681 acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg
1741 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca
1801 acaacaattg cattcatttt atgtttcagg ttcaggggga gatgtgggag gttttttaaa
1861 gcaagtaaaa cctctacaaa tgtggtaaaa tccgataagg atcgatccgg gctggcgtaa
1921 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
1981 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
2041 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttcacgcc
2101 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
2161 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
2221 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
2281 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
2341 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
2401 aacgcgaatt ttaacaaaat attaacgctt acaatttcct gatgcggtat tttctcctta
2461 cgcatctgtg cggtatttca caccgcatac gcggatcttc cgtaccttct gaggcggaaa
2521 gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
2581 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
2641 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
2701 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
2761 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
2821 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc ttgattcttc
2881 tgacacaaca gtctcgaact taaggctaga attctggcta caggtaagcg cccctaaaat
2941 ccctttgggc acaatgtgtc ctgaggggag aggcagcgac ctgtagatgg gacgggggca
3001 ctaaccctca ggtttggggc ttctgaatgt gagtatcgcc atgtaagccc agtatttggc
3061 caatctcaga aagctcctgg tccctggagg gatggagaga gaaaaacaaa cagctcctgg
3121 agcagggaga gtgctggcct cttgctctcc ggctccctct gttgccctct ggtttctccc
3181 caggctcccg gacgtcctct agccaccatg acttcgaaag tttatgatcc agaacaaagg
3241 aaacggatga taactggtcc gcagtggtgg gccagatgta aacaaatgaa tgttcttgat
3301 tcatttatta attattatga ttcagaaaaa catgcagaaa atgctgttat ttttttacat
3361 ggtaacgcgg cctcttctta tttatggcga catgttgtgc cacatattga gccagtagcg
3421 cggtgtatta taccagacct tattggtatg ggcaaatcag gcaaatctgg taatggttct
3481 tataggttac ttgatcatta caaatatctt actgcatggt ttgaacttct taatttacca
3541 aagaagatca tttttgtcgg ccatgattgg ggtgcttgtt tggcatttca ttatagctat
3601 gagcatcaag ataagatcaa agcaatagtt cacgctgaaa gtgtagtaga tgtgattgaa
3661 tcatgggatg aatggcctga tattgaagaa gatattgcgt tgatcaaatc tgaagaagga
3721 gaaaaaatgg ttttggagaa taacttcttc gtggaaacca tgttgccatc aaaaatcatg
3781 agaaagttag aaccagaaga atttgcagca tatcttgaac cattcaaaga gaaaggtgaa
3841 gttcgtcgtc caacattatc atggcctcgt gaaatcccgt tagtaaaagg tggtaaacct
3901 gacgttgtac aaattgttag gaattataat gcttatctac gtgcaagtga tgatttacca
3961 aaaatgttta ttgaatcgga cccaggattc ttttccaatg ctattgttga aggtgccaag
4021 aagtttccta atactgaatt tgtcaaagta aaaggtcttc atttttcgca agaagatgca
4081 cctgatgaaa tgggaaaata tatcaaatcg ttcgttgagc gagttctcaa aaatgaacaa
4141 taattctagc cctgaataag tgataataag cggatgaatg gcagaaattc gtcgaagcgc
4201 aataaaatat ctttattttc attacatctg tgtgttggtt ttttgtgtga atcgatagcg
4261 ataaggatcg gaagatccgc gtatggtgca ctctcagtac aatctgctct gatgccgcat
4321 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
4381 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
4441 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat
4501 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
4561 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
4621 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
4681 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
4741 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
4801 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
4861 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg
4921 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
4981 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
5041 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
5101 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
5161 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
5221 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
5281 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
5341 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
5401 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
5461 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
5521 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
5581 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
5641 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
5701 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
5761 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
5821 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
5881 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
5941 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
6001 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
6061 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
6121 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
6181 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
6241 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
6301 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatggct cgacagatct
//