LOCUS Exported 3494 bp ds-DNA circular SYN 29-DEC-2017
DEFINITION synthetic circular DNA
FEATURES Location/Qualifiers
source 1..3494
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 196..224
/label=tet promoter
/note="E. coli promoter for tetracycline efflux protein
gene"
CDS 272..1462
/codon_start=1
/gene="tet"
/product="tetracycline efflux protein"
/label=TcR
/note="confers resistance to tetracycline"
/translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
promoter complement(1637..1655)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
rep_origin 2343..2931
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
terminator 3261..3288
/label=rrnB T2 terminator
/note="transcription terminator T2 from the E. coli rrnB
gene"
terminator 3380..3466
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
ORIGIN
1 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
61 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
121 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
181 aattaattct catgtttgac agcttatcat cgattagctt taatgcggta gtttatcaca
241 gttaaattgc taacgcagtc aggcaccgtg tatgaaatct aacaatgcgc tcatcgtcat
301 cctcggcacc gtcaccctgg atgctgtagg cataggcttg gttatgccgg tactgccggg
361 cctcttgcgg gatatcgtcc attccgacag catcgccagt cactatggcg tgctgctagc
421 gctatatgcg ttgatgcaat ttctatgcgc acccgttctc ggagcactgt ccgaccgctt
481 tggccgccgc ccagtcctgc tcgcttcgct acttggagcc actatcgact acgcgatcat
541 ggcgaccaca cccgtcctgt ggattctcta cgccggacgc atcgtggccg gcatcaccgg
601 cgccacaggt gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc
661 tcgccacttc gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc
721 cgggggactg ttgggcgcca tctccttaca tgcaccattc cttgcggcgg cggtgctcaa
781 cggcctcaac ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgccg
841 acccatgccc ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac
901 tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc
961 agcgctctgg gtcattttcg gcgaggaccg ctttcgctgg agcgcgacga tgatcggcct
1021 gtcgcttgcg gtattcggaa tcttgcacgc cctcgctcaa gccttcgtca ctggtcccgc
1081 caccaaacgt ttcggcgaga agcaggccat tatcgccggc atggcggccg acgcgctggg
1141 ctacgtcttg ctggcgttcg cgacgcgagg ctggatggcc ttccccatta tgattcttct
1201 cgcttccggc ggcatcggga tgcccgcgtt gcaggccatg ctgtccaggc aggtagatga
1261 cgaccatcag ggacagcttc aaggatcgct cgcggctctt accagcctaa cttcgatcat
1321 tggaccgctg atcgtcacgg cgatttatgc cgcctcggcg agcacatgga acgggttggc
1381 atggattgta ggcgccgccc tataccttgt ctgcctcccc gcgttgcgtc gcggtgcatg
1441 gagccgggcc acctcgacct gaatggaagc cggcggcacc tcgctaacgg attcaccact
1501 ccaagaattg gagccaatca attcttgcgg agaactgtga atgcgcaaac caacccttga
1561 tcggggaaga acagtatgtc gagctatttt ttgacttact ggggatcaag cctgattggg
1621 agaaaataaa atatccccta tagtgagtcg tattacatgg tcatagctgt ttcctggcag
1681 ctctggcccg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa tatatcatca
1741 tgcctcctct agaggtctca ggaccgcggc cgctccttca ggcactgccg acgacagccc
1801 aggcggaggt cctgagcgcc ggcgccaaat ttgcataaag agctacccag aagccctcgc
1861 gcgcggaaac gggcaaaaaa gggcttctaa tatggaaata ttacgccgaa tcgcgtcaca
1921 aattggctaa gcgggcctaa gagttaacaa gatgtgctat taagcggagc cttttggtgg
1981 gaagaaacgg aacagtcact gtgttctaaa agaacttgca ggatgagcct ttaaataccg
2041 cagtctcgac gctcttagtc ggagacgttg atttcgtctc tgtttaagag ctatgctgga
2101 aacagcatag caagtttaaa taaggctagt ccgttatcaa cttgaaaaag tggcaccgag
2161 tcggtgcttt tttgttttcc atattttttt cgcattgctt tccttggctt ttacaccctt
2221 acgcagcctc gccccctttt cccagggaga ccctcgagcc acccatgacc aaaatccctt
2281 aacgtgagtt acgcgtcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
2341 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
2401 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
2461 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
2521 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
2581 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
2641 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
2701 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
2761 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
2821 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
2881 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
2941 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
3001 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
3061 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
3121 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
3181 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atacgcgtac cgctagccag
3241 gaagagtttg tagaaacgca aaaaggccat ccgtcaggat ggccttctgc ttagtttgat
3301 gcctggcagt ttatggcggg cgtcctgccc gccaccctcc gggccgttgc ttcacaacgt
3361 tcaaatccgc tcccggcgga tttgtcctac tcaggagagc gttcaccgac aaacaacaga
3421 taaaacgaaa ggcccagtct tccgactgag cctttcgttt tatttgatgc ctggcagttc
3481 cctactctcg cgtt
//