LOCUS Exported 13129 bp ds-DNA circular SYN 07-APR-2017
DEFINITION synthetic circular DNA
FEATURES Location/Qualifiers
source 1..13129
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 238..617
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 619..817
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
LTR 835..1015
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 1062..1187
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1680..1913
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
misc_feature 2440..2557
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 2608..2848
/label=U6 promoter
/note="RNA polymerase III promoter for human U6 snRNA"
misc_RNA 2873..2948
/label=g RNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
promoter 3010..3221
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
CDS 3246..7349
/codon_start=1
/product="Cas9 (Csn1) endonuclease from the Streptococcus
pyogenes Type II CRISPR/Cas system"
/label=Cas9
/note="generates RNA-guided double strand breaks in DNA"
/translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
DATLIHQSITGLYETRIDLSQLGGD"
CDS 7350..7397
/codon_start=1
/product="bipartite nuclear localization signal from
nucleoplasmin"
/label=nucleoplasmin NLS
/translation="KRPAATKKAGQAKKKK"
CDS 7398..7421
/codon_start=1
/product="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
/label=FLAG
/translation="DYKDDDDK"
CDS 7431..7487
/codon_start=1
/product="2A peptide from porcine teschovirus-1
polyprotein"
/label=P2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="ATNFSLLKQAGDVEENPGP"
misc_feature 7488..8207
/label=GFP
misc_feature 8221..8809
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
CDS complement(8692..8703)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 8881..9114
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
polyA_signal 9146..9370
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 9416..9844
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 9858..10187
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 10038..10173
/label=SV40 ori
/note="SV40 origin of replication"
promoter 10235..10282
/label=EM7 promoter
/note="synthetic bacterial promoter "
CDS 10301..10675
/codon_start=1
/gene="Sh ble from Streptoalloteichus hindustanus"
/product="antibiotic-binding protein"
/label=BleoR
/note="confers resistance to bleomycin, phleomycin, and
Zeocin(TM)"
/translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
EFALRDPAGNCVHFVAEEQD"
polyA_signal 10805..10926
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(10975..10991)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 10999..11015
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(11023..11053)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 11068..11089
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(11377..11965)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(12136..12996)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(12997..13101)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
2581 agatccagtt tggttaatta aggtaccgag ggcctatttc ccatgattcc ttcatatttg
2641 catatacgat acaaggctgt tagagagata attagaatta atttgactgt aaacacaaag
2701 atattagtac aaaatacgtg acgtagaaag taataatttc ttgggtagtt tgcagtttta
2761 aaattatgtt ttaaaatgga ctatcatatg cttaccgtaa cttgaaagta tttcgatttc
2821 ttggctttat atatcttgtg gaaaggacga aacaccggag acggacgtct ctgttttaga
2881 gctagaaata gcaagttaaa ataaggctag tccgttatca acttgaaaaa gtggcaccga
2941 gtcggtgctt ttttgaattc gctagctagg tcttgaaagg agtgggaatt ggctccggtg
3001 cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg
3061 gcaattgatc cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt
3121 actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg
3181 tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggaccggttc tagagcgctg
3241 ccaccatgga caagaagtac agcatcggcc tggacatcgg caccaactct gtgggctggg
3301 ccgtgatcac cgacgagtac aaggtgccca gcaagaaatt caaggtgctg ggcaacaccg
3361 accggcacag catcaagaag aacctgatcg gagccctgct gttcgacagc ggcgaaacag
3421 ccgaggccac ccggctgaag agaaccgcca gaagaagata caccagacgg aagaaccgga
3481 tctgctatct gcaagagatc ttcagcaacg agatggccaa ggtggacgac agcttcttcc
3541 acagactgga agagtccttc ctggtggaag aggataagaa gcacgagcgg caccccatct
3601 tcggcaacat cgtggacgag gtggcctacc acgagaagta ccccaccatc taccacctga
3661 gaaagaaact ggtggacagc accgacaagg ccgacctgcg gctgatctat ctggccctgg
3721 cccacatgat caagttccgg ggccacttcc tgatcgaggg cgacctgaac cccgacaaca
3781 gcgacgtgga caagctgttc atccagctgg tgcagaccta caaccagctg ttcgaggaaa
3841 accccatcaa cgccagcggc gtggacgcca aggccatcct gtctgccaga ctgagcaaga
3901 gcagacggct ggaaaatctg atcgcccagc tgcccggcga gaagaagaat ggcctgttcg
3961 gaaacctgat tgccctgagc ctgggcctga cccccaactt caagagcaac ttcgacctgg
4021 ccgaggatgc caaactgcag ctgagcaagg acacctacga cgacgacctg gacaacctgc
4081 tggcccagat cggcgaccag tacgccgacc tgtttctggc cgccaagaac ctgtccgacg
4141 ccatcctgct gagcgacatc ctgagagtga acaccgagat caccaaggcc cccctgagcg
4201 cctctatgat caagagatac gacgagcacc accaggacct gaccctgctg aaagctctcg
4261 tgcggcagca gctgcctgag aagtacaaag agattttctt cgaccagagc aagaacggct
4321 acgccggcta cattgacggc ggagccagcc aggaagagtt ctacaagttc atcaagccca
4381 tcctggaaaa gatggacggc accgaggaac tgctcgtgaa gctgaacaga gaggacctgc
4441 tgcggaagca gcggaccttc gacaacggca gcatccccca ccagatccac ctgggagagc
4501 tgcacgccat tctgcggcgg caggaagatt tttacccatt cctgaaggac aaccgggaaa
4561 agatcgagaa gatcctgacc ttccgcatcc cctactacgt gggccctctg gccaggggaa
4621 acagcagatt cgcctggatg accagaaaga gcgaggaaac catcaccccc tggaacttcg
4681 aggaagtggt ggacaagggc gcttccgccc agagcttcat cgagcggatg accaacttcg
4741 ataagaacct gcccaacgag aaggtgctgc ccaagcacag cctgctgtac gagtacttca
4801 ccgtgtataa cgagctgacc aaagtgaaat acgtgaccga gggaatgaga aagcccgcct
4861 tcctgagcgg cgagcagaaa aaggccatcg tggacctgct gttcaagacc aaccggaaag
4921 tgaccgtgaa gcagctgaaa gaggactact tcaagaaaat cgagtgcttc gactccgtgg
4981 aaatctccgg cgtggaagat cggttcaacg cctccctggg cacataccac gatctgctga
5041 aaattatcaa ggacaaggac ttcctggaca atgaggaaaa cgaggacatt ctggaagata
5101 tcgtgctgac cctgacactg tttgaggaca gagagatgat cgaggaacgg ctgaaaacct
5161 atgcccacct gttcgacgac aaagtgatga agcagctgaa gcggcggaga tacaccggct
5221 ggggcaggct gagccggaag ctgatcaacg gcatccggga caagcagtcc ggcaagacaa
5281 tcctggattt cctgaagtcc gacggcttcg ccaacagaaa cttcatgcag ctgatccacg
5341 acgacagcct gacctttaaa gaggacatcc agaaagccca ggtgtccggc cagggcgata
5401 gcctgcacga gcacattgcc aatctggccg gcagccccgc cattaagaag ggcatcctgc
5461 agacagtgaa ggtggtggac gagctcgtga aagtgatggg ccggcacaag cccgagaaca
5521 tcgtgatcga aatggccaga gagaaccaga ccacccagaa gggacagaag aacagccgcg
5581 agagaatgaa gcggatcgaa gagggcatca aagagctggg cagccagatc ctgaaagaac
5641 accccgtgga aaacacccag ctgcagaacg agaagctgta cctgtactac ctgcagaatg
5701 ggcgggatat gtacgtggac caggaactgg acatcaaccg gctgtccgac tacgatgtgg
5761 accatatcgt gcctcagagc tttctgaagg acgactccat cgacaacaag gtgctgacca
5821 gaagcgacaa gaaccggggc aagagcgaca acgtgccctc cgaagaggtc gtgaagaaga
5881 tgaagaacta ctggcggcag ctgctgaacg ccaagctgat tacccagaga aagttcgaca
5941 atctgaccaa ggccgagaga ggcggcctga gcgaactgga taaggccggc ttcatcaaga
6001 gacagctggt ggaaacccgg cagatcacaa agcacgtggc acagatcctg gactcccgga
6061 tgaacactaa gtacgacgag aatgacaagc tgatccggga agtgaaagtg atcaccctga
6121 agtccaagct ggtgtccgat ttccggaagg atttccagtt ttacaaagtg cgcgagatca
6181 acaactacca ccacgcccac gacgcctacc tgaacgccgt cgtgggaacc gccctgatca
6241 aaaagtaccc taagctggaa agcgagttcg tgtacggcga ctacaaggtg tacgacgtgc
6301 ggaagatgat cgccaagagc gagcaggaaa tcggcaaggc taccgccaag tacttcttct
6361 acagcaacat catgaacttt ttcaagaccg agattaccct ggccaacggc gagatccgga
6421 agcggcctct gatcgagaca aacggcgaaa ccggggagat cgtgtgggat aagggccggg
6481 attttgccac cgtgcggaaa gtgctgagca tgccccaagt gaatatcgtg aaaaagaccg
6541 aggtgcagac aggcggcttc agcaaagagt ctatcctgcc caagaggaac agcgataagc
6601 tgatcgccag aaagaaggac tgggacccta agaagtacgg cggcttcgac agccccaccg
6661 tggcctattc tgtgctggtg gtggccaaag tggaaaaggg caagtccaag aaactgaaga
6721 gtgtgaaaga gctgctgggg atcaccatca tggaaagaag cagcttcgag aagaatccca
6781 tcgactttct ggaagccaag ggctacaaag aagtgaaaaa ggacctgatc atcaagctgc
6841 ctaagtactc cctgttcgag ctggaaaacg gccggaagag aatgctggcc tctgccggcg
6901 aactgcagaa gggaaacgaa ctggccctgc cctccaaata tgtgaacttc ctgtacctgg
6961 ccagccacta tgagaagctg aagggctccc ccgaggataa tgagcagaaa cagctgtttg
7021 tggaacagca caagcactac ctggacgaga tcatcgagca gatcagcgag ttctccaaga
7081 gagtgatcct ggccgacgct aatctggaca aagtgctgtc cgcctacaac aagcaccggg
7141 ataagcccat cagagagcag gccgagaata tcatccacct gtttaccctg accaatctgg
7201 gagcccctgc cgccttcaag tactttgaca ccaccatcga ccggaagagg tacaccagca
7261 ccaaagaggt gctggacgcc accctgatcc accagagcat caccggcctg tacgagacac
7321 ggatcgacct gtctcagctg ggaggcgaca agcgacctgc cgccacaaag aaggctggac
7381 aggctaagaa gaagaaagat tacaaagacg atgacgataa gggatccggc gcaacaaact
7441 tctctctgct gaaacaagcc ggagatgtcg aagagaatcc tggaccgatg gtgtctaagg
7501 gcgaagagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
7561 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
7621 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
7681 tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct
7741 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
7801 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
7861 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
7921 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga
7981 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
8041 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
8101 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
8161 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaatag ttaagtcgac
8221 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
8281 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
8341 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
8401 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
8461 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
8521 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
8581 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
8641 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
8701 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
8761 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcg tcgactttaa
8821 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
8881 tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc
8941 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
9001 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
9061 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagggccc
9121 gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc
9181 ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa
9241 aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg
9301 gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg
9361 ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg
9421 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
9481 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
9541 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
9601 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
9661 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
9721 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
9781 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
9841 aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag
9901 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
9961 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
10021 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
10081 atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat
10141 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag
10201 cttgtatatc cattttcgga tctgatcagc acgtgttgac aattaatcat cggcatagta
10261 tatcggcata gtataatacg acaaggtgag gaactaaacc atggccaagt tgaccagtgc
10321 cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga ccgaccggct
10381 cgggttctcc cgggacttcg tggaggacga cttcgccggt gtggtccggg acgacgtgac
10441 cctgttcatc agcgcggtcc aggaccaggt ggtgccggac aacaccctgg cctgggtgtg
10501 ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg
10561 ggacgcctcc gggccggcca tgaccgagat cggcgagcag ccgtgggggc gggagttcgc
10621 cctgcgcgac ccggccggca actgcgtgca cttcgtggcc gaggagcagg actgacacgt
10681 gctacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
10741 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
10801 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
10861 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
10921 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata
10981 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
11041 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
11101 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
11161 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
11221 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
11281 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
11341 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
11401 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
11461 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
11521 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
11581 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
11641 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
11701 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
11761 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
11821 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
11881 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
11941 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
12001 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
12061 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
12121 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
12181 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
12241 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
12301 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
12361 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
12421 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
12481 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
12541 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
12601 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
12661 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
12721 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
12781 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
12841 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
12901 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
12961 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
13021 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
13081 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgac
//