LOCUS Exported 14538 bp ds-DNA circular SYN 04-DEC-2017
DEFINITION synthetic circular DNA
FEATURES Location/Qualifiers
source 1..14538
/organism="synthetic DNA construct"
/mol_type="other DNA"
LTR 533..766
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
polyA_signal 798..1022
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 1068..1496
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 1510..1839
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 1690..1825
/label=SV40 ori
/note="SV40 origin of replication"
promoter 1887..1934
/label=EM7 promoter
/note="synthetic bacterial promoter "
CDS 1953..2327
/codon_start=1
/gene="Sh ble from Streptoalloteichus hindustanus"
/product="antibiotic-binding protein"
/label=Zeo
/note="confers resistance to bleomycin, phleomycin, and
Zeocin(TM)"
/translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
EFALRDPAGNCVHFVAEEQD"
polyA_signal 2457..2578
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(3029..3617)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(3788..4648)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(4649..4753)
/gene="bla"
/label=AmpR promoter
enhancer 5019..5398
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 5400..5598
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
LTR 5616..5796
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 5843..5968
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 6461..6694
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
misc_feature 7221..7338
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 7458..7669
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
regulatory 7688..7697
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 7694..11797
/codon_start=1
/product="catalytically dead mutant of the Cas9
endonuclease from the Streptococcus pyogenes Type II
CRISPR/Cas system"
/label=dCas9
/note="RNA-guided DNA-binding protein that lacks
endonuclease activity due to the D10A mutation in the RuvC
catalytic domain and the H840A mutation in the HNH
catalytic domain"
/translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
DATLIHQSITGLYETRIDLSQLGGD"
CDS 11798..11845
/codon_start=1
/product="bipartite nuclear localization signal from
nucleoplasmin"
/label=nucleoplasmin NLS
/translation="KRPAATKKAGQAKKKK"
CDS 11852..13702
/codon_start=1
/gene="EP300"
/product="catalytic histone acetyltransferase core of the
human E1A-associated protein p300 (Delvecchio et al.,
2013)"
/label=p300 core
/translation="IFKPEELRQALMPTLEALYRQDPESLPFRQPVDPQLLGIPDYFDI
VKSPMDLSTIKRKLDTGQYQEPWQYVDDIWLMFNNAWLYNRKTSRVYKYCSKLSEVFEQ
EIDPVMQSLGYCCGRKLEFSPQTLCCYGKQLCTIPRDATYYSYQNRYHFCEKCFNEIQG
ESVSLGDDPSQPQTTINKEQFSKRKNDTLDPELFVECTECGRKMHQICVLHHEIIWPAG
FVCDGCLKKSARTRKENKFSAKRLPSTRLGTFLENRVNDFLRRQNHPESGEVTVRVVHA
SDKTVEVKPGMKARFVDSGEMAESFPYRTKALFAFEEIDGVDLCFFGMHVQEYGSDCPP
PNQRRVYISYLDSVHFFRPKCLRTAVYHEILIGYLEYVKKLGYTTGHIWACPPSEGDDY
IFHCHPPDQKIPKPKRLQEWYKKMLDKAVSERIVHDYKDIFKQATEDRLTSAKELPYFE
GDFWPNVLEESIKELEQEEEERKREENTSNESTDVTKGDSKNAKKKNNKKTSKNKSSLS
RGNKKKPGMPNVSNDLSQKLYATMEKHKEVFFVIRLIAGPAANSLPPIVDPDPLIPCDL
MDGRDAFLTLARDKHLEFSSLRRAQWSTMCMLVELHTQSQD"
CDS 13709..13732
/codon_start=1
/product="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
/label=FLAG
/translation="DYKDDDDK"
CDS 13742..13798
/codon_start=1
/product="2A peptide from porcine teschovirus-1
polyprotein"
/label=P2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="ATNFSLLKQAGDVEENPGP"
CDS 13799..14395
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
misc_feature 14411..461
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
ORIGIN
1 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
61 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
121 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
181 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
241 tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt
301 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
361 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
421 ccctcagacg agtcggatct ccctttgggc cgcctccccg cgtcgacttt aagaccaatg
481 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg
541 ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta
601 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
661 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
721 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagggc ccgtttaaac
781 ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
841 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
901 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
961 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
1021 ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag
1081 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
1141 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
1201 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
1261 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
1321 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
1381 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
1441 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt
1501 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
1561 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
1621 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
1681 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
1741 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag
1801 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata
1861 tccattttcg gatctgatca gcacgtgttg acaattaatc atcggcatag tatatcggca
1921 tagtataata cgacaaggtg aggaactaaa ccatggccaa gttgaccagt gccgttccgg
1981 tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg ctcgggttct
2041 cccgggactt cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg accctgttca
2101 tcagcgcggt ccaggaccag gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg
2161 gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct
2221 ccgggccggc catgaccgag atcggcgagc agccgtgggg gcgggagttc gccctgcgcg
2281 acccggccgg caactgcgtg cacttcgtgg ccgaggagca ggactgacac gtgctacgag
2341 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg
2401 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact
2461 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
2521 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
2581 atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc
2641 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
2701 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
2761 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
2821 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
2881 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
2941 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
3001 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
3061 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
3121 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
3181 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
3241 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
3301 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
3361 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
3421 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
3481 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
3541 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
3601 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
3661 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
3721 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
3781 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
3841 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
3901 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
3961 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
4021 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
4081 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
4141 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
4201 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
4261 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
4321 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
4381 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
4441 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
4501 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
4561 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
4621 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
4681 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
4741 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc
4801 tcccgatccc ctatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
4861 gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca aaatttaagc
4921 tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttaggg ttaggcgttt
4981 tgcgctgctt cgcgatgtac gggccagata tacgcgttga cattgattat tgactagtta
5041 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
5101 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
5161 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
5221 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
5281 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
5341 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
5401 gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc
5461 aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt
5521 tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg
5581 ggaggtctat ataagcagcg cgttttgcct gtactgggtc tctctggtta gaccagatct
5641 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
5701 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
5761 tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa
5821 agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac
5881 ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta
5941 gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg
6001 ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg
6061 ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg
6121 ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag aagaacttag
6181 atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga
6241 caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca
6301 gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg
6361 aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa
6421 agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt
6481 tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca
6541 gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc
6601 aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg
6661 ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac
6721 tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga
6781 tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa
6841 tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg
6901 aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata
6961 taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac
7021 tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc
7081 caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca
7141 gagacagatc cattcgatta gtgaacggat cggcactgcg tgcgccaatt ctgcagacaa
7201 atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg
7261 gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt
7321 acaaaaattc aaaattttcg ggtttattac agggacagca gagatccagt ttggttaatt
7381 aaggtaccga ggcgataggg ttaagggagc tagctaggtc ttgaaaggag tgggaattgg
7441 ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg
7501 aggggtcggc aattgatccg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga
7561 tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt
7621 agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg accggttcta
7681 gagcgctgcc accatggaca agaagtacag catcggcctg gccatcggca ccaactctgt
7741 gggctgggcc gtgatcaccg acgagtacaa ggtgcccagc aagaaattca aggtgctggg
7801 caacaccgac cggcacagca tcaagaagaa cctgatcgga gccctgctgt tcgacagcgg
7861 cgaaacagcc gaggccaccc ggctgaagag aaccgccaga agaagataca ccagacggaa
7921 gaaccggatc tgctatctgc aagagatctt cagcaacgag atggccaagg tggacgacag
7981 cttcttccac agactggaag agtccttcct ggtggaagag gataagaagc acgagcggca
8041 ccccatcttc ggcaacatcg tggacgaggt ggcctaccac gagaagtacc ccaccatcta
8101 ccacctgaga aagaaactgg tggacagcac cgacaaggcc gacctgcggc tgatctatct
8161 ggccctggcc cacatgatca agttccgggg ccacttcctg atcgagggcg acctgaaccc
8221 cgacaacagc gacgtggaca agctgttcat ccagctggtg cagacctaca accagctgtt
8281 cgaggaaaac cccatcaacg ccagcggcgt ggacgccaag gccatcctgt ctgccagact
8341 gagcaagagc agacggctgg aaaatctgat cgcccagctg cccggcgaga agaagaatgg
8401 cctgttcgga aacctgattg ccctgagcct gggcctgacc cccaacttca agagcaactt
8461 cgacctggcc gaggatgcca aactgcagct gagcaaggac acctacgacg acgacctgga
8521 caacctgctg gcccagatcg gcgaccagta cgccgacctg tttctggccg ccaagaacct
8581 gtccgacgcc atcctgctga gcgacatcct gagagtgaac accgagatca ccaaggcccc
8641 cctgagcgcc tctatgatca agagatacga cgagcaccac caggacctga ccctgctgaa
8701 agctctcgtg cggcagcagc tgcctgagaa gtacaaagag attttcttcg accagagcaa
8761 gaacggctac gccggctaca ttgacggcgg agccagccag gaagagttct acaagttcat
8821 caagcccatc ctggaaaaga tggacggcac cgaggaactg ctcgtgaagc tgaacagaga
8881 ggacctgctg cggaagcagc ggaccttcga caacggcagc atcccccacc agatccacct
8941 gggagagctg cacgccattc tgcggcggca ggaagatttt tacccattcc tgaaggacaa
9001 ccgggaaaag atcgagaaga tcctgacctt ccgcatcccc tactacgtgg gccctctggc
9061 caggggaaac agcagattcg cctggatgac cagaaagagc gaggaaacca tcaccccctg
9121 gaacttcgag gaagtggtgg acaagggcgc ttccgcccag agcttcatcg agcggatgac
9181 caacttcgat aagaacctgc ccaacgagaa ggtgctgccc aagcacagcc tgctgtacga
9241 gtacttcacc gtgtataacg agctgaccaa agtgaaatac gtgaccgagg gaatgagaaa
9301 gcccgccttc ctgagcggcg agcagaaaaa ggccatcgtg gacctgctgt tcaagaccaa
9361 ccggaaagtg accgtgaagc agctgaaaga ggactacttc aagaaaatcg agtgcttcga
9421 ctccgtggaa atctccggcg tggaagatcg gttcaacgcc tccctgggca cataccacga
9481 tctgctgaaa attatcaagg acaaggactt cctggacaat gaggaaaacg aggacattct
9541 ggaagatatc gtgctgaccc tgacactgtt tgaggacaga gagatgatcg aggaacggct
9601 gaaaacctat gcccacctgt tcgacgacaa agtgatgaag cagctgaagc ggcggagata
9661 caccggctgg ggcaggctga gccggaagct gatcaacggc atccgggaca agcagtccgg
9721 caagacaatc ctggatttcc tgaagtccga cggcttcgcc aacagaaact tcatgcagct
9781 gatccacgac gacagcctga cctttaaaga ggacatccag aaagcccagg tgtccggcca
9841 gggcgatagc ctgcacgagc acattgccaa tctggccggc agccccgcca ttaagaaggg
9901 catcctgcag acagtgaagg tggtggacga gctcgtgaaa gtgatgggcc ggcacaagcc
9961 cgagaacatc gtgatcgaaa tggccagaga gaaccagacc acccagaagg gacagaagaa
10021 cagccgcgag agaatgaagc ggatcgaaga gggcatcaaa gagctgggca gccagatcct
10081 gaaagaacac cccgtggaaa acacccagct gcagaacgag aagctgtacc tgtactacct
10141 gcagaatggg cgggatatgt acgtggacca ggaactggac atcaaccggc tgtccgacta
10201 cgatgtggac gccatcgtgc ctcagagctt tctgaaggac gactccatcg acaacaaggt
10261 gctgaccaga agcgacaaga accggggcaa gagcgacaac gtgccctccg aagaggtcgt
10321 gaagaagatg aagaactact ggcggcagct gctgaacgcc aagctgatta cccagagaaa
10381 gttcgacaat ctgaccaagg ccgagagagg cggcctgagc gaactggata aggccggctt
10441 catcaagaga cagctggtgg aaacccggca gatcacaaag cacgtggcac agatcctgga
10501 ctcccggatg aacactaagt acgacgagaa tgacaagctg atccgggaag tgaaagtgat
10561 caccctgaag tccaagctgg tgtccgattt ccggaaggat ttccagtttt acaaagtgcg
10621 cgagatcaac aactaccacc acgcccacga cgcctacctg aacgccgtcg tgggaaccgc
10681 cctgatcaaa aagtacccta agctggaaag cgagttcgtg tacggcgact acaaggtgta
10741 cgacgtgcgg aagatgatcg ccaagagcga gcaggaaatc ggcaaggcta ccgccaagta
10801 cttcttctac agcaacatca tgaacttttt caagaccgag attaccctgg ccaacggcga
10861 gatccggaag cggcctctga tcgagacaaa cggcgaaacc ggggagatcg tgtgggataa
10921 gggccgggat tttgccaccg tgcggaaagt gctgagcatg ccccaagtga atatcgtgaa
10981 aaagaccgag gtgcagacag gcggcttcag caaagagtct atcctgccca agaggaacag
11041 cgataagctg atcgccagaa agaaggactg ggaccctaag aagtacggcg gcttcgacag
11101 ccccaccgtg gcctattctg tgctggtggt ggccaaagtg gaaaagggca agtccaagaa
11161 actgaagagt gtgaaagagc tgctggggat caccatcatg gaaagaagca gcttcgagaa
11221 gaatcccatc gactttctgg aagccaaggg ctacaaagaa gtgaaaaagg acctgatcat
11281 caagctgcct aagtactccc tgttcgagct ggaaaacggc cggaagagaa tgctggcctc
11341 tgccggcgaa ctgcagaagg gaaacgaact ggccctgccc tccaaatatg tgaacttcct
11401 gtacctggcc agccactatg agaagctgaa gggctccccc gaggataatg agcagaaaca
11461 gctgtttgtg gaacagcaca agcactacct ggacgagatc atcgagcaga tcagcgagtt
11521 ctccaagaga gtgatcctgg ccgacgctaa tctggacaaa gtgctgtccg cctacaacaa
11581 gcaccgggat aagcccatca gagagcaggc cgagaatatc atccacctgt ttaccctgac
11641 caatctggga gcccctgccg ccttcaagta ctttgacacc accatcgacc ggaagaggta
11701 caccagcacc aaagaggtgc tggacgccac cctgatccac cagagcatca ccggcctgta
11761 cgagacacgg atcgacctgt ctcagctggg aggcgacaag cgacctgccg ccacaaagaa
11821 ggctggacag gctaagaaga agaaaggatc cattttcaaa ccagaagaac tacgacaggc
11881 actgatgcca actttggagg cactttaccg tcaggatcca gaatcccttc cctttcgtca
11941 acctgtggac cctcagcttt taggaatccc tgattacttt gatattgtga agagccccat
12001 ggatctttct accattaaga ggaagttaga cactggacag tatcaggagc cctggcagta
12061 tgtcgatgat atttggctta tgttcaataa tgcctggtta tataaccgga aaacatcacg
12121 ggtatacaaa tactgctcca agctctctga ggtctttgaa caagaaattg acccagtgat
12181 gcaaagcctt ggatactgtt gtggcagaaa gttggagttc tctccacaga cactgtgttg
12241 ctacggcaaa cagttgtgca caatacctcg tgatgccact tattacagtt accagaacag
12301 gtatcatttc tgtgagaagt gtttcaatga gatccaaggg gagagcgttt ctttggggga
12361 tgacccttcc cagcctcaaa ctacaataaa taaagaacaa ttttccaaga gaaaaaatga
12421 cacactggat cctgaactgt ttgttgaatg tacagagtgc ggaagaaaga tgcatcagat
12481 ctgtgtcctt caccatgaga tcatctggcc tgctggattc gtctgtgatg gctgtttaaa
12541 gaaaagtgca cgaactagga aagaaaataa gttttctgct aaaaggttgc catctaccag
12601 acttggcacc tttctagaga atcgtgtgaa tgactttctg aggcgacaga atcaccctga
12661 gtcaggagag gtcactgtta gagtagttca tgcttctgac aaaaccgtgg aagtaaaacc
12721 aggcatgaaa gcaaggtttg tggacagtgg agagatggca gaatcctttc cataccgaac
12781 caaagccctc tttgcctttg aagaaattga tggtgttgac ctgtgcttct ttggcatgca
12841 tgttcaagag tatggctctg actgccctcc acccaaccag aggagagtat acatatctta
12901 cctcgatagt gttcatttct tccgtcctaa atgcttgagg actgcagtct atcatgaaat
12961 cctaattgga tatttagaat atgtcaagaa attaggttac acaacagggc atatttgggc
13021 atgtccacca agtgagggag atgattatat cttccattgc catcctcctg accagaagat
13081 acccaagccc aagcgactgc aggaatggta caaaaaaatg cttgacaagg ctgtatcaga
13141 gcgtattgtc catgactaca aggatatttt taaacaagct actgaagata gattaacaag
13201 tgcaaaggaa ttgccttatt tcgagggtga tttctggccc aatgttctgg aagaaagcat
13261 taaggaactg gaacaggagg aagaagagag aaaacgagag gaaaacacca gcaatgaaag
13321 cacagatgtg accaagggag acagcaaaaa tgctaaaaag aagaataata agaaaaccag
13381 caaaaataag agcagcctga gtaggggcaa caagaagaaa cccgggatgc ccaatgtatc
13441 taacgacctc tcacagaaac tatatgccac catggagaag cataaagagg tcttctttgt
13501 gatccgcctc attgctggcc ctgctgccaa ctccctgcct cccattgttg atcctgatcc
13561 tctcatcccc tgcgatctga tggatggtcg ggatgcgttt ctcacgctgg caagggacaa
13621 gcacctggag ttctcttcac tccgaagagc ccagtggtcc accatgtgca tgctggtgga
13681 gctgcacacg cagagccagg acggatccga ttacaaagac gatgacgata agggatccgg
13741 cgcaacaaac ttctctctgc tgaaacaagc cggagatgtc gaagagaatc ctggaccgac
13801 cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac
13861 cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca
13921 catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg
13981 caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt
14041 cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg
14101 gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc
14161 gtggttcctg gccaccgtcg gagtctcgcc cgaccaccag ggcaagggtc tgggcagcgc
14221 cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac
14281 ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt
14341 cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgaacgcg
14401 ttaagtcgac aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
14461 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
14521 tgcttcccgt atggcttt
//