LOCUS Exported 4555 bp ds-DNA circular SYN 05-NOV-2017
DEFINITION synthetic circular DNA
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4555)
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..4555
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 402..492
/label=H1 promoter
/note="human H1 RNA promoter"
misc_feature 501..558
/label=Stuffer
rep_origin complement(979..1567)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(1738..2598)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
polyA_signal 2751..2872
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
CDS complement(3002..4027)
/codon_start=1
/gene="aph(4)-Ia"
/product="aminoglycoside phosphotransferase from E. coli"
/label=HygR
/note="confers resistance to hygromycin"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
E"
promoter complement(4084..4294)
/label=SV40 promoter
/note="SV40 early promoter"
rep_origin 4090..4225
/label=SV40 ori
/note="SV40 origin of replication"
ORIGIN
1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt catatttgca tgtcgctatg
421 tgttctggga aatcaccata aacgtgaaat gtctttggat ttgggaatct tataagttct
481 gtatgagacc actcggatcc cagctcacaa ccaggatact ttcaagagaa gtatcctggt
541 tgtgagcttt ttttggaaaa gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
601 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
661 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
721 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
781 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
841 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
901 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
961 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
1021 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
1081 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
1141 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
1201 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
1261 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
1321 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
1381 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
1441 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
1501 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
1561 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
1621 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
1681 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
1741 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
1801 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
1861 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
1921 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
1981 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
2041 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
2101 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
2161 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
2221 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
2281 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
2341 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
2401 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
2461 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
2521 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
2581 gaaatgttga atactcatac tcttcctttt tcaatattca ggctttacac tttatgcttc
2641 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
2701 accatgatta cgccaagctc tagctagagg tcgacggtat acagacatga taagatacat
2761 tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat
2821 ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag ttggggtggg
2881 cgaagaactc cagcatgaga tccccgcgct ggaggatcat ccagccggcg tcccggaaaa
2941 cgattccgaa gcccaacctt tcatagaagg cggcggtgga atcgaaatct cgtagcacgt
3001 gctattcctt tgccctcgga cgagtgctgg ggcgtcggtt tccactatcg gcgagtactt
3061 ctacacagcc atcggtccag acggccgcgc ttctgcgggc gatttgtgta cgcccgacag
3121 tcccggctcc ggatcggacg attgcgtcgc atcgaccctg cgcccaagct gcatcatcga
3181 aattgccgtc aaccaagctc tgatagagtt ggtcaagacc aatgcggagc atatacgccc
3241 ggagccgcgg cgatcctgca agctccggat gcctccgctc gaagtagcgc gtctgctgct
3301 ccatacaagc caaccacggc ctccagaaga agatgttggc gacctcgtat tgggaatccc
3361 cgaacatcgc ctcgctccag tcaatgaccg ctgttatgcg gccattgtcc gtcaggacat
3421 tgttggagcc gaaatccgcg tgcacgaggt gccggacttc ggggcagtcc tcggcccaaa
3481 gcatcagctc atcgagagcc tgcgcgacgg acgcactgac ggtgtcgtcc atcacagttt
3541 gccagtgata cacatgggga tcagcaatcg cgcatatgaa atcacgccat gtagtgtatt
3601 gaccgattcc ttgcggtccg aatgggccga acccgctcgt ctggctaaga tcggccgcag
3661 cgatcgcatc catggcctcc gcgaccggct gcagaacagc gggcagttcg gtttcaggca
3721 ggtcttgcaa cgtgacaccc tgtgcacggc gggagatgca ataggtcagg ctctcgctga
3781 attccccaat gtcaagcact tccggaatcg ggagcgcggc cgatgcaaag tgccgataaa
3841 cataacgatc tttgtagaaa ccatcggcgc agctatttac ccgcaggaca tatccacgcc
3901 ctcctacatc gaagctgaaa gcacgagatt cttcgccctc cgagagctgc atcaggtcgg
3961 agacgctgtc gaacttttcg atcagaaact tctcgacaga cgtcgcggtg agttcaggct
4021 ttttcatcac gtgctgatca gatccgaaaa tggatataca agctcccggg agctttttgc
4081 aaaagcctag gcctccaaaa aagcctcctc actacttctg gaatagctca gaggcagagg
4141 cggcctcggc ctctgcataa ataaaaaaaa ttagtcagcc atggggcgga gaatgggcgg
4201 aactgggcgg agttaggggc gggatgggcg gagttagggg cgggactatg gttgctgact
4261 aattgagatg catgcttcgc atacttctgc ctgctgggga gcctggggac tttccacacc
4321 aggttgctga ctaattgaga tgcatgcttt gcatacttct gcctgctgga atattattga
4381 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
4441 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
4501 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtc
//