LOCUS Exported 5159 bp ds-DNA circular SYN 29-AUG-2016
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS Untitled
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5159)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Monday, August 29, 2016 from SnapGene Viewer 3.1.4
http://www.miaollingbio.com
FEATURES Location/Qualifiers
source 1..5159
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 235..614
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 615..818
/note="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
promoter 863..881
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 905..967
/codon_start=1
/product="leader sequence from mouse immunoglobulin kappa
light chain"
/note="Ig-kappa leader"
/translation="METDTLLLWVLLLWVPGSTGD"
CDS 1082..1111
/codon_start=1
/product="Myc (human c-Myc oncogene) epitope tag"
/note="Myc"
/translation="EQKLISEEDL"
CDS 1127..1144
/codon_start=1
/product="6xHis affinity tag"
/note="6xHis"
/translation="HHHHHH"
polyA_signal 1173..1397
/note="bGH poly(A) signal"
/note="bovine growth hormone polyadenylation signal"
rep_origin 1443..1871
/direction=RIGHT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 1885..2214
/note="SV40 promoter"
/note="SV40 enhancer and early promoter"
rep_origin 2065..2200
/note="SV40 ori"
/note="SV40 origin of replication"
promoter 2262..2309
/note="EM7 promoter"
/note="synthetic bacterial promoter "
CDS 2328..2702
/codon_start=1
/gene="Sh ble from Streptoalloteichus hindustanus"
/product="antibiotic-binding protein"
/note="BleoR"
/note="confers resistance to bleomycin, phleomycin, and
Zeocin(TM)"
/translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
EFALRDPAGNCVHFVAEEQD"
polyA_signal 2832..2953
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
primer_bind complement(3002..3018)
/note="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 3026..3042
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(3050..3080)
/note="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind 3095..3116
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(3404..3992)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(4163..5023)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(5024..5128)
/gene="bla"
/note="AmpR promoter"
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 caccatggag acagacacac tcctgctatg ggtactgctg ctctgggttc caggttccac
961 tggtgacgcg gcccagccgg ccaggcgcgc cgtacgaagc ttggtaccga gctcggatcc
1021 actccagtgt ggtggaattc tgcagatatc cagcacagtg gcggccgctc gaggagggcc
1081 cgaacaaaaa ctcatctcag aagaggatct gaatagcgcc gtcgaccatc atcatcatca
1141 tcattgagtt taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt
1201 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
1261 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
1321 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga
1381 tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc
1441 ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
1501 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
1561 cacgttcgcc ggctttcccc gtcaagctct aaatcggggc atccctttag ggttccgatt
1621 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
1681 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
1741 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
1801 ataagggatt ttggggattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
1861 taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
1921 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
1981 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
2041 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
2101 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct
2161 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc
2221 ccgggagctt gtatatccat tttcggatct gatcagcacg tgttgacaat taatcatcgg
2281 catagtatat cggcatagta taatacgaca aggtgaggaa ctaaaccatg gccaagttga
2341 ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg
2401 accggctcgg gttctcccgg gacttcgtgg aggacgactt cgccggtgtg gtccgggacg
2461 acgtgaccct gttcatcagc gcggtccagg accaggtggt gccggacaac accctggcct
2521 gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg gtcggaggtc gtgtccacga
2581 acttccggga cgcctccggg ccggccatga ccgagatcgg cgagcagccg tgggggcggg
2641 agttcgccct gcgcgacccg gccggcaact gcgtgcactt cgtggccgag gagcaggact
2701 gacacgtgct acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa
2761 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct
2821 tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
2881 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
2941 tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat
3001 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
3061 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
3121 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
3181 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
3241 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
3301 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
3361 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
3421 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
3481 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
3541 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcaat
3601 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
3661 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
3721 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
3781 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
3841 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
3901 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
3961 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
4021 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
4081 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
4141 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
4201 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
4261 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
4321 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
4381 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
4441 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
4501 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
4561 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
4621 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
4681 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
4741 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
4801 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
4861 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
4921 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
4981 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
5041 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
5101 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtc
//