LOCUS Exported 4392 bp ds-DNA circular SYN 15-JUL-2016
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pAdVAntage
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4392)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Tuesday, August 30, 2016 from SnapGene Viewer 3.1.4
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..4392
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(1743..1759)
/note="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 1767..1783
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(1791..1821)
/note="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind 1836..1857
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(2145..2733)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(2904..3764)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(3765..3869)
/gene="bla"
/note="AmpR promoter"
primer_bind 4343..4359
/note="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
ORIGIN
1 gtcgacagaa gcaccatgtc cttgggtccg gcctgctgaa tgcgcaggcg gtcggccatg
61 ccccaggctt cgttttgaca tcggcgcagg tctttgtagt agtcttgcat gagcctttct
121 accggcactt cttcttctcc ttcctcttgt cctgcatctc ttgcatctat cgctacggcg
181 gcggcggagt ttggccgtag gtggcgccct cttcctccca tgcgtgtgac cccgaagccc
241 ctcatcggct gaagcagggc caggtcggcg acaacgcgct cggctaatat ggcctgctgc
301 acctgcgtga gggtagactg gaagtcatcc atgtccacaa agcggtggta tgcgcccgtg
361 ttgatggtgt aagtgcagtt ggccataacg gaccagttaa cggtctggtg acccggctgc
421 gagagctcgg tgtacctgag acgcgagtaa gcccttgagt caaagacgta gtcgttgcaa
481 gtccgcacca ggtactgata tcccaccaaa aagtgcggcg gcggctggcg gtagaggggc
541 cagcgtaggg tggccggggc tccgggggcg aggtcttcca acataaggcg atgatatccg
601 tagatgtacc tggacatcca ggtgatgccg gcggcggtgg tggaggcgcg cggaaagtcg
661 cggacgcggt tccagatgtt gcgcagcggc aaaaagtgct ccatggtcgg gacgctctgg
721 ccggtgaggc gtgcgcagtc gttgacgctc tagaccgtgc aaaaggagag cctgtaagcg
781 ggcactcttc cgtggtctgg tggataaatt cgcaagggta tcatggcgga cgaccggggt
841 tcgaaccccg gatccggccg tccgccgtga tccatgcggt taccgcccgc gtgtcgaacc
901 caggtgtgcg acgtcagaca acgggggagc gctccttttg gcttccttcc aggcgcggcg
961 gctgctgcgc tagctttttt ggccactggc cgcgcgcggc gtaagcggtt aggctggaaa
1021 gcgaaagcat taagtggctc gctccctgta gccggagggt tattttccaa gggttgagtc
1081 gcaggacccc cggttcgagt ctcgggccgg ccggactgcg gcgaacgggg gtttgcctcc
1141 ccgtcatgca agaccccgct tgcaaattcc tccggaaaca gggacgagcc ccttttttgc
1201 ttttcccaga tgcatccggt gctgcggcag atgcgccccc ctcctcagca gcggcaagag
1261 caagagcagc ggcagacatg cagggcaccc tccccttctc ctaccgcgtc aggaggggca
1321 acatccgcgg ctgacgcggc ggcagatggt gattacgaac ccccgcggcg ccgggcccgg
1381 cactacctgg acttggagga gggcgagggc ctggcgcggc taggagcgcc ctctcctgag
1441 cgacacccaa gggtgcagct gaagcgtgac acgcgcgagg cgtacgtgcc gcggcagaac
1501 ctgtttcgcg accgcgaggg agaggagccc gaggagatgc gggatcgaaa gttccacgca
1561 gggcgcgagt tgcggcatgg cctgaaccgc gagcggttgc tgcgcgagga ggactttgag
1621 cccgacgcgc ggaccgggat tagtcccgcg cgcgcacacg tggcggccgc cgacctggta
1681 accgcgtacg agcagacggt gaaccaggag attaactttc aaaaaagctt ggcgtaatca
1741 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
1801 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
1861 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
1921 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
1981 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
2041 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
2101 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
2161 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
2221 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
2281 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
2341 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
2401 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
2461 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
2521 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
2581 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
2641 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
2701 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
2761 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
2821 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
2881 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
2941 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
3001 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
3061 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
3121 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
3181 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
3241 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
3301 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
3361 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
3421 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
3481 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
3541 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
3601 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
3661 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
3721 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
3781 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
3841 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
3901 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
3961 cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
4021 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
4081 ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga
4141 gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg
4201 cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg
4261 ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca
4321 gggttttccc agtcacgacg ttgtaaaacg acggccagtg aattcgagct cggtacccgg
4381 ggatcctcta ga
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