LOCUS Exported 3431 bp ds-DNA circular SYN 05-SEP-2016
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS Untitled 5
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3431)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Monday, September 5, 2016 from SnapGene Viewer 3.1.4
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..3431
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 4..382
/note="TRE3G promoter"
/note="3rd-generation Tet-responsive promoter that can be
activated by binding of Tet-On(R) 3G"
protein_bind 12..30
/gene="tetO"
/bound_moiety="tetracycline repressor TetR"
/note="tet operator"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 48..66
/gene="tetO"
/bound_moiety="tetracycline repressor TetR"
/note="tet operator"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 84..102
/gene="tetO"
/bound_moiety="tetracycline repressor TetR"
/note="tet operator"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 120..138
/gene="tetO"
/bound_moiety="tetracycline repressor TetR"
/note="tet operator"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 156..174
/gene="tetO"
/bound_moiety="tetracycline repressor TetR"
/note="tet operator"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 192..210
/gene="tetO"
/bound_moiety="tetracycline repressor TetR"
/note="tet operator"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 228..246
/gene="tetO"
/bound_moiety="tetracycline repressor TetR"
/note="tet operator"
/note="bacterial operator O2 for the tetR and tetA genes"
intron 583..648
/note="small t intron"
/note="simian virus 40 (SV40) small t antigen intron"
CDS 778..798
/codon_start=1
/product="nuclear localization signal of SV40 large T
antigen"
/note="SV40 NLS"
/translation="PKKKRKV"
polyA_signal 1223..1344
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin complement(1601..2189)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(2360..3220)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(3221..3325)
/gene="bla"
/note="AmpR promoter"
ORIGIN
1 ctcgagttta ctccctatca gtgatagaga acgtatgaag agtttactcc ctatcagtga
61 tagagaacgt atgcagactt tactccctat cagtgataga gaacgtataa ggagtttact
121 ccctatcagt gatagagaac gtatgaccag tttactccct atcagtgata gagaacgtat
181 ctacagttta ctccctatca gtgatagaga acgtatatcc agtttactcc ctatcagtga
241 tagagaacgt ataagcttta ggcgtgtacg gtgggcgcct ataaaagcag agctcgttta
301 gtgaaccgtc agatcgcctg gagcaattcc acaacacttt tgtcttatac caactttccg
361 taccacttcc taccctcgta aagtcgacac cggggcccag atctatcgat cggccggata
421 tcacgcgtca tatggctagc ctgcagggat ccaatgtaac tgtattcagc gatgacgaaa
481 ttcttagcta ttgtaatact ctagaggatc tttgtgaagg aaccttactt ctgtggtgtg
541 acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta
601 agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat
661 ggaactgatg aatgggagca gtggtggaat gcctttaatg aggaaaacct gttttgctca
721 gaagaaatgc catctagtga tgatgaggct actgctgact ctcaacattc tactcctcca
781 aaaaagaaga gaaaggtaga agaccccaag gactttcctt cagaattgct aagttttttg
841 agtcatgctg tgtttagtaa tagaactctt gcttgctttg ctatttacac cacaaaggaa
901 aaagctgcac tgctatacaa gaaaattatg gaaaaatatt ctgtaacctt tataagtagg
961 cataacagtt ataatcataa catactgttt tttcttactc cacacaggca tagagtgtct
1021 gctattaata actatgctca aaaattgtgt acctttagct ttttaatttg taaaggggtt
1081 aataaggaat atttgatgta tagtgccttg actagagatc ataatcagcc ataccacatt
1141 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
1201 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
1261 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
1321 gtccaaactc atcaatgtat cttatcatgt ctgcggctct agagctgcat taatgaatcg
1381 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
1441 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
1501 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
1561 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
1621 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
1681 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
1741 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
1801 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
1861 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
1921 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
1981 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
2041 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
2101 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
2161 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
2221 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
2281 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
2341 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
2401 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
2461 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
2521 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
2581 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
2641 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
2701 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
2761 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
2821 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
2881 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
2941 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
3001 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
3061 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
3121 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
3181 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
3241 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
3301 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag
3361 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
3421 ttcaagaatt c
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