LOCUS Exported 9126 bp ds-DNA circular SYN 08-DEC-2017
DEFINITION synthetic circular DNA
FEATURES Location/Qualifiers
source 1..9126
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_signal 198..348
/label=Ad5 Psi
/note="packaging signal for adenovirus serotype 5"
polyA_signal 491..612
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
CDS complement(760..1437)
/codon_start=1
/product="monomeric derivative of DsRed (Campbell et al.,
2002)"
/label=mRFP1
/note="mammalian codon-optimized"
/translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
TGA"
promoter complement(1480..1683)
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
polyA_signal 2046..2167
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
promoter complement(2445..2648)
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
enhancer 2649..2952
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
rep_origin complement(6523..7111)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS 7918..8712
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
ORIGIN
1 ttaattaatc ccttccagct ctctgcccct tttggattga agccaatatg ataatgaggg
61 ggtggagttt gtgacgtggc gcgggcgtgg gaacggggcg ggtgacgtag tagtgtggcg
121 gaagtgtgat gttgcaagtg tggcggaaca catgtaagcg acggatgtgg caaaagtgac
181 gtttttggtg tgcgccggtg tacacaggaa gtgacaattt tcgcgcggtt ttaggcggat
241 gttgtagtaa atttgggcgt aaccgagtaa gatttggcca ttttcgcggg aaaactgaat
301 aagaggaagt gaaatctgaa taattttgtg ttactcatag cgcgtaatat ttgtctaggg
361 ccgcggggac tttgaccgtt tacgtggaga ctcgcccagg tgtttttctc aggtgttttc
421 cgcgttccgg gtcaaagttg gcgttttatt attatagtca gtcgagtcta gcaagctagc
481 ttgatcgcgt taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa
541 aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc
601 aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg
661 tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga ttatgatctt
721 gtggtatggc tgattatgat ctagagtcgc ggccaattct taggcgccgg tggagtggcg
781 gccctcggcg cgctcgtact gttccacgat ggtgtagtcc tcgttgtggg aggtgatgtc
841 cagcttgatg tcggtcttgt aggcgccggg cagctgcacg ggcttcttgg ccatgtaggt
901 ggtcttgacc tcggcgtcgt agtggccgcc gtccttcagc ttcagcctca tcttgatctc
961 gcccttcagg gcgccgtcct cggggtacat ccgctcggtg gaggcctccc agcccatggt
1021 cttcttctgc attacggggc cgtcggaggg gaagttggtg ccgcgcagct tcaccttgta
1081 gatgaactcg ccgtcctgca gggaggagtc ctgggtcacg gtcaccacgc cgccgtcctc
1141 gaagttcatc acgcgctccc acttgaagcc ctcggggaag gacagcttca agtagtcggg
1201 gatgtcggcg gggtgcttca cgtaggcctt ggagccgtac tggaactgag gggacaggat
1261 gtcccaggcg aagggcaggg ggccgccctt ggtcaccttc agcttggcgg tctgggtgcc
1321 ctcgtagggg cggccctcgc cctcgccctc gatctcgaac tcgtggccgt tcacggagcc
1381 ctccatgcgc accttgaagc gcatgaactc cttgatgacg tcctcggagg aggccatcgg
1441 atcccggtag cgctagcgga tctgacggtt cactaaacca gctctgctta tatagacctc
1501 ccaccgtaca cgcctaccgc ccatttgcgt caatggggcg gagttgttac gacattttgg
1561 aaagtcccgt tgattttggt gccaaaacaa actcccattg acgtcaatgg ggtggagact
1621 tggaaatccc cgtgagtcaa accgctatcc acgcccattg atgtactgcc aaaaccgcat
1681 caccatggta atagcgatga ctaatacgta gatgtactgc caagtaggaa agtcccataa
1741 ggtcatgtac tgggcataat gccaggcggg ccatttaccg tcattgacgt caataggggg
1801 cgtacttggc atatgataca cttgatgtac tgccaagtgg gcagtttacc gtaaatactc
1861 cacccattga cgtcaatgga aagtccctat tggcgttact atgggaacat acgtcattat
1921 tgacgtcaat gggcgggggt cgttgggcgg tcagccaggc gggccattta ccgtaagtta
1981 tgtaacgcgg aactccatat atgggctatg aactaatgac cccgtaattg attactatta
2041 cgcgttaaga tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg
2101 ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa
2161 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga
2221 ggttttttaa agcaagtaaa acctctacaa atgtggtatg gctgattatg atcagttatc
2281 tagatccggt ggatctgagt ccggacttgt acagctcgtc catgccgaga gtgatcccgg
2341 cggcggtcac gaactccaga tcggatatct tatctagaag cttaggctcg agcggccgcg
2401 tcgacggtac cagatctcta gcggatctga cggttcacta aaccagctct gcttatatag
2461 acctcccacc gtacacgcct accgcccatt tgcgtcaatg gggcggagtt gttacgacat
2521 tttggaaagt cccgttgatt ttggtgccaa aacaaactcc cattgacgtc aatggggtgg
2581 agacttggaa atccccgtga gtcaaaccgc tatccacgcc cattgatgta ctgccaaaac
2641 cgcatcacca tggtaatagc gatgactaat acgtagatgt actgccaagt aggaaagtcc
2701 cataaggtca tgtactgggc ataatgccag gcgggccatt taccgtcatt gacgtcaata
2761 gggggcgtac ttggcatatg atacacttga tgtactgcca agtgggcagt ttaccgtaaa
2821 tactccaccc attgacgtca atggaaagtc cctattggcg ttactatggg aacatacgtc
2881 attattgacg tcaatgggcg ggggtcgttg ggcggtcagc caggcgggcc atttaccgta
2941 agttatgtaa cgcggaactc catatatggg ctatgaacta atgaccccgt aattgattac
3001 tattagacgt ttatcactgc tagaggatct gggcgtggtt aagggtggga aagaatatat
3061 aaggtggggg tcttatgtag ttttgtatct gttttgcagc agccgccgcc gccatgagca
3121 ccaactcgtt tgatggaagc attgtgagct catatttgac aacgcgcatg cccccatggg
3181 ccggggtgcg tcagaatgtg atgggctcca gcattgatgg tcgccccgtc ctgcccgcaa
3241 actctactac cttgacctac gagaccgtgt ctggaacgcc gttggagact gcagcctccg
3301 ccgccgcttc agccgctgca gccaccgccc gcgggattgt gactgacttt gctttcctga
3361 gcccgcttgc aagcagtgca gcttcccgtt catccgcccg cgatgacaag ttgacggctc
3421 ttttggcaca attggattct ttgacccggg aacttaatgt cgtttctcag cagctgttgg
3481 atctgcgcca gcaggtttct gccctgaagg cttcctcccc tcccaatgcg gtttaaaaca
3541 taaataaaaa accagactct gtttggattt ggatcaagca agtgtcttgc tgtctttatt
3601 taggggtttt gcgcgcgcgg taggcccggg accagcggtc tcggtcgttg agggtcctgt
3661 gtattttttc caggacgtgg taaaggtgac tctggatgtt cagatacatg ggcataagcc
3721 cgtctctggg gtggaggtag caccactgca gagcttcatg ctgcggggtg gtgttgtaga
3781 tgatccagtc gtagcaggag cgctgggcgt ggtgcctaaa aatgtctttc agtagcaagc
3841 tgattgccag gggcaggccc ttggtgtaag tgtttacaaa gcggttaagc tgggatgggt
3901 gcatacgtgg ggatatgaga tgcatcttgg actgtatttt taggttggct atgttcccag
3961 ccatatccct ccggggattc atgttgtgca gaaccaccag cacagtgtat ccggtgcact
4021 tgggaaattt gtcatgtagc ttagaaggaa atgcgtggaa gaacttggag acgcccttgt
4081 gacctccaag attttccatg cattcgtcca taatgatggc aatgggccca cgggcggcgg
4141 cctgggcgaa gatatttctg ggatcactaa cgtcatagtt gtgttccagg atgagatcgt
4201 cataggccat ttttacaaag cgcgggcgga gggtgccaga ctgcggtata atggttccat
4261 ccggcccagg ggcgtagtta ccctcacaga tttgcatttc ccacgctttg agttcagatg
4321 gggggatcat gtctacctgc ggggcgatga agaaaacggt ttccggggta ggggagatca
4381 gctgggaaga aagcaggttc ctgagcagct gcgacttacc gcagccggtg ggcccgtaaa
4441 tcacacctat taccgggtgc aactggtagt taagagagct gcagctgccg tcatccctga
4501 gcaggggggc cacttcgtta agcatgtccc tgactcgcat gttttccctg accaaatccg
4561 ccagaaggcg ctcgccgccc agcgatagca gttcttgcaa ggaagcaaag tttttcaacg
4621 gtttgagacc gtccgccgta ggcatgcttt tgagcgtttg accaagcagt tccaggcggt
4681 cccacagctc ggtcacctgc tctacggcat ctcgatccag catatctcct cgtttcgcgg
4741 gttggggcgg ctttcgctgt acggcagtag tcggtgctcg tccagacggg ccagggtcat
4801 gtctttccac gggcgcaggg tcctcgtcag cgtagtctgg gtcacggtga aggggtgcgc
4861 tccgggctgc gcgctggcca gggtgcgctt gaggctggtc ctgctggtgc tgaagcgctg
4921 ccggtcttcg ccctgcgcgt cggccaggta gcatttgacc atggtgtcat agtccagccc
4981 ctccgcggcg tggcccttgg cgcgcagctt gcccttggag gaggcgccgc acgaggggca
5041 gtgcagactt ttgagggcgt agagcttggg cgcgagaaat accgattccg gggagtaggc
5101 atccgcgccg caggccccgc agacggtctc gcattccacg agccaggtga gctctggccg
5161 ttcggggtca aaaaccaggt ttcccccatg ctttttgatg cgtttcttac ctctggtttc
5221 catgagccgg tgtccacgct cggtgacgaa aaggctgtcc gtgtccccgt atacagactg
5281 tttaaacgaa ttctataaaa tgcaaggtgc tgctcaaaaa atcaggcaaa gcctcgcgca
5341 aaaaagaaag cacatcgtag tcatgctcat gcagataaag gcaggtaagc tccggaacca
5401 ccacagaaaa agacaccatt tttctctcaa acatgtctgc gggtttctgc ataaacacaa
5461 aataaaataa caaaaaaaca tttaaacatt agaagcctgt cttacaacag gaaaaacaac
5521 ccttataagc ataagacgga ctacggccat gccggcgtga ccgtaaaaaa actggtcacc
5581 gtgattaaaa agcaccaccg acagctcctc ggtcatgtcc ggagtcataa tgtaagactc
5641 ggtaaacaca tcaggttgat tcatcggtca gtgctaaaaa gcgaccgaaa tagcccgggg
5701 gaatacatac ccgcaggcgt agagacaaca ttacagcccc cataggaggt ataacaaaat
5761 taataggaga gaaaaacaca taaacacctg aaaaaccctc ctgcctaggc aaaatagcac
5821 cctcccgctc cagaacaaca tacagcgctt cacagcggca gcctaacagt cagccttacc
5881 agtaaaaaag aaaacctatt aaaaaaacac cactcgacac ggcaccagct caatcagtca
5941 cagtgtaaaa aagggccaag tgcagagcga gtatatatag gactaaaaaa tgacgtaacg
6001 gttaaagtcc acaaaaaaca cccagaaaac cgcacgcgaa cctacgccca gaaacgaaag
6061 ccaaaaaacc cacaacttcc tcaaatcgtc acttccgttt tcccacgtta cgtaacttcc
6121 cattttaaga aaactacaat tcccaacaca tacaagttac tccgccctaa aacctacgtc
6181 acccgccccg ttcccacgcc ccgcgccacg tcacaaactc caccccctca ttatcatatt
6241 ggcttcaatc caaaataagg tatattattg atgatttaat taaggatccc ggtgtgaaat
6301 accgcacaga tgcgtaagga gaaaataccg catcaggcgc tcttccgctt cctcgctcac
6361 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
6421 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
6481 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
6541 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
6601 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
6661 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
6721 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
6781 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
6841 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
6901 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
6961 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
7021 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
7081 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
7141 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
7201 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
7261 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
7321 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
7381 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
7441 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
7501 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
7561 gccagttaat agtttgcgca acgttgttga aaaaggatct tcacctagat ccttttcacg
7621 tagaaagcca gtccgcagaa acggtgctga ccccggatga atgtcagcta ctgggctatc
7681 tggacaaggg aaaacgcaag cgcaaagaga aagcaggtag cttgcagtgg gcttacatgg
7741 cgatagctag actgggcggt tttatggaca gcaagcgaac cggaattgcc agctggggcg
7801 ccctctggta aggttgggaa gccctgcaaa gtaaactgga tggctttctc gccgccaagg
7861 atctgatggc gcaggggatc aagctctgat caagagacag gatgaggatc gtttcgcatg
7921 attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc
7981 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg
8041 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa
8101 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc
8161 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat
8221 ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
8281 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc
8341 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag
8401 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgagcat gcccgacggc
8461 gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc
8521 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata
8581 gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc
8641 gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac
8701 gagttcttct gaattttgtt aaaatttttg ttaaatcagc tcatttttta accaataggc
8761 cgaaatcggc aacatccctt ataaatcaaa agaatagacc gcgatagggt tgagtgttgt
8821 tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa
8881 aaccgtctat cagggcgatg gcccactacg tgaaccatca cccaaatcaa gttttttgcg
8941 gtcgaggtgc cgtaaagctc taaatcggaa ccctaaaggg agcccccgat ttagagcttg
9001 acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc
9061 tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg cgcgcttaat
9121 gcgccg
//