LOCUS Exported 6215 bp ds-DNA circular SYN 05-NOV-2017
DEFINITION synthetic circular DNA
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6215)
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..6215
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_signal 1..353
/label=Ad5
repeat_region 16..118
/label=ITR
/note="inverted terminal repeat of human adenovirus
serotype 5"
enhancer 385..688
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 689..892
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
misc_feature 913..1002
/label=MCS
polyA_signal 1014..1148
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
misc_feature 1437..3901
/label=AD5
rep_origin complement(4388..4976)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(5147..6007)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(6008..6112)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 aattaattaa gctagcatca tcaataatat accttatttt ggattgaagc caatatgata
61 atgagggggt ggagtttgtg acgtggcgcg gggcgtggga acggggcggg tgacgtagta
121 gtgtggcgga agtgtgatgt tgcaagtgtg gcggaacaca tgtaagcgac ggatgtggca
181 aaagtgacgt ttttggtgtg cgccggtgta cacaggaagt gacaattttc gcgcggtttt
241 aggcggatgt tgtagtaaat ttgggcgtaa ccgagtaaga tttggccatt ttcgcgggaa
301 aactgaataa gaggaagtga aatctgaata attttgtgtt actcatagcg cgtaatattt
361 gtctagggag atcagcctgc aggtcgttac ataacttacg gtaaatggcc cgcctggctg
421 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
481 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
541 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
601 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
661 ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg
721 tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag
781 tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt
841 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctcgtttagt
901 gaaccgtcag ttggtaccgt ttaaactcga ggtcgacggt atcgataagc ttgatatcga
961 attcctgcag cccgggggat ccactagttc tagagcggcc gccacagcgg ggagatccag
1021 acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat
1081 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata
1141 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg
1201 aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat gatccggctg
1261 cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
1321 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
1381 tgttggcggg tgtcggggcg cagccatgag gtcgactcta gtccccgcgg tggcagatct
1441 ggaaggtgct gaggtacgat gagacccgca ccaggtgcag accctgcgag tgtggcggta
1501 aacatattag gaaccagcct gtgatgctgg atgtgaccga ggagctgagg cccgatcact
1561 tggtgctggc ctgcacccgc gctgagtttg gctctagcga tgaagataca gattgaggta
1621 ctgaaatgtg tgggcgtggc ttaagggtgg gaaagaatat ataaggtggg ggtcttatgt
1681 agttttgtat ctgttttgca gcagccgccg ccgccatgag caccaactcg tttgatggaa
1741 gcattgtgag ctcatatttg acaacgcgca tgcccccatg ggccggggtg cgtcagaatg
1801 tgatgggctc cagcattgat ggtcgccccg tcctgcccgc aaactctact accttgacct
1861 acgagaccgt gtctggaacg ccgttggaga ctgcagcctc cgccgccgct tcagccgctg
1921 cagccaccgc ccgcgggatt gtgactgact ttgctttcct gagcccgctt gcaagcagtg
1981 cagcttcccg ttcatccgcc cgcgatgaca agttgacggc tcttttggca caattggatt
2041 ctttgacccg ggaacttaat gtcgtttctc agcagctgtt ggatctgcgc cagcaggttt
2101 ctgccctgaa ggcttcctcc cctcccaatg cggtttaaaa cataaataaa aaaccagact
2161 ctgtttggat ttggatcaag caagtgtctt gctgtcttta tttaggggtt ttgcgcgcgc
2221 ggtaggcccg ggaccagcgg tctcggtcgt tgagggtcct gtgtattttt tccaggacgt
2281 ggtaaaggtg actctggatg ttcagataca tgggcataag cccgtctctg gggtggaggt
2341 agcaccactg cagagcttca tgctgcgggg tggtgttgta gatgatccag tcgtagcagg
2401 agcgctgggc gtggtgccta aaaatgtctt tcagtagcaa gctgattgcc aggggcaggc
2461 ccttggtgta agtgtttaca aagcggttaa gctgggatgg gtgcatacgt ggggatatga
2521 gatgcatctt ggactgtatt tttaggttgg ctatgttccc agccatatcc ctccggggat
2581 tcatgttgtg cagaaccacc agcacagtgt atccggtgca cttgggaaat ttgtcatgta
2641 gcttagaagg aaatgcgtgg aagaacttgg agacgccctt gtgacctcca agattttcca
2701 tgcattcgtc cataatgatg gcaatgggcc cacgggcggc ggcctgggcg aagatatttc
2761 tgggatcact aacgtcatag ttgtgttcca ggatgagatc gtcataggcc atttttacaa
2821 agcgcgggcg gagggtgcca gactgcggta taatggttcc atccggccca ggggcgtagt
2881 taccctcaca gatttgcatt tcccacgctt tgagttcaga tggggggatc atgtctacct
2941 gcggggcgat gaagaaaacg gtttccgggg taggggagat cagctgggaa gaaagcaggt
3001 tcctgagcag ctgcgactta ccgcagccgg tgggcccgta aatcacacct attaccgggt
3061 gcaactggta gttaagagag ctgcagctgc cgtcatccct gagcaggggg gccacttcgt
3121 taagcatgtc cctgactcgc atgttttccc tgaccaaatc cgccagaagg cgctcgccgc
3181 ccagcgatag cagttcttgc aaggaagcaa agtttttcaa cggtttgaga ccgtccgccg
3241 taggcatgct tttgagcgtt tgaccaagca gttccaggcg gtcccacagc tcggtcacct
3301 gctctacggc atctcgatcc agcatatctc ctcgtttcgc gggttggggc ggctttcgct
3361 gtacggcagt agtcggtgct cgtccagacg ggccagggtc atgtctttcc acgggcgcag
3421 ggtcctcgtc agcgtagtct gggtcacggt gaaggggtgc gctccgggct gcgcgctggc
3481 cagggtgcgc ttgaggctgg tcctgctggt gctgaagcgc tgccggtctt cgccctgcgc
3541 gtcggccagg tagcatttga ccatggtgtc atagtccagc ccctccgcgg cgtggccctt
3601 ggcgcgcagc ttgcccttgg aggaggcgcc gcacgagggg cagtgcagac ttttgagggc
3661 gtagagcttg ggcgcgagaa ataccgattc cggggagtag gcatccgcgc cgcaggcccc
3721 gcagacggtc tcgcattcca cgagccaggt gagctctggc cgttcggggt caaaaaccag
3781 gtttccccca tgctttttga tgcgtttctt acctctggtt tccatgagcc ggtgtccacg
3841 ctcggtgacg aaaaggctgt ccgtgtcccc gtatacagac ttgagaggcc tgtcctcgac
3901 cgatgccctt gagagccttc aacccagtca gctccttccg gtgggcgcgg ggcatgacta
3961 tcgtcgccgc acttatgact gtcttcttta tcatgcaact cgtaggacag gtgccggcag
4021 cgctctgggt cattttcggc gaggaccgct ttcgctggag cgcgacgatg atcggcctgt
4081 cgcttgcggt attcggaatc ttgcacgccc tcgctcaagc cttcgtcact ggtcccgcca
4141 ccaaacgttt cggcgagaag caggccatta tcgccggcat ggcggccgac gcgctgggct
4201 acgtcttgct ggcgttcgcg acgcgaggct ggatggcctt ccccattatg attcttctcg
4261 cttccggcgg catcgggatg cccgcgttgc aggccatgct gtccaggcag gtagatgacg
4321 accatcaggg acagcttcaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
4381 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
4441 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
4501 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
4561 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
4621 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
4681 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
4741 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
4801 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
4861 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
4921 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
4981 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
5041 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
5101 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
5161 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
5221 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
5281 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
5341 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
5401 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
5461 ctgcaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
5521 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
5581 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
5641 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
5701 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
5761 caacacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
5821 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
5881 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
5941 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
6001 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
6061 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
6121 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
6181 ataggcgtat cacgaggccc tttcgtcttc aagaa
//