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pacAd5 9.2-100

pacAd5 9.2-100
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  • 产品名称:pacAd5 9.2-100
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  • 产品展商:XYbscience
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简单介绍
pacAd5 9.2-100菌株发货前均经过严格的多重验证,如存在质量问题,请在收到产品的三个月内通知我司。pacAd5 9.2-100收到质粒后请短暂离心,加入20μl ddH2O溶解质粒,取2μl��化至对应感受态中,挑取单克隆重新提取质粒后使用。
产品描述

pacAd5 9.2-100

  • 产品信息
  • 基本信息
  • 质粒简介
  • 质粒图谱
  • 质粒序列

产品信息

产品货号 产品名称 产品规格 优惠价
XY1618 pacAd5 9.2-100

20μl

¥请加QQ询价

使用说明

信裕质粒平台的各批次质粒菌株发货前均经过严格的多重验证,如存在质量问题,请在收到产品的三个月内通知我司。收到质粒后请短暂离心,加入20μl ddH2O溶解质粒,取2μl转化至对应感受态中,挑取单克隆重新提取质粒后使用。如需获取其他详细信息请登录我司官网查询。

基本信息

终止子: SV40 poly(A) signal
质粒大小: 34947bp
原核抗性: 氨苄青霉素Amp
克隆菌株: 大肠杆菌Stbl3
培养条件: 37℃,有氧,LB

质粒简介

          pacAd5 9.2-100是一个腺病毒骨架质粒,用于产生腺病毒,pacAd5 9.2-100质粒
         Recombinant adenoviruses have tremendous potential in both research and therapeutic applications.There are numerous advantages they provide when introducing genetic material into host cells. The permissive host cell range is very wide. The virus has been used to infect many mammalian cell types (both replicative and non-replicative) for high expression of the recombinant protein. Recombinant adenoviruses are especially useful for gene transfer and protein expression in cell lines that have low transfection efficiency with liposome. After entering cells, the virus remains epichromosomal (i.e.does not integrate into the host chromosome so does not activate or inactivate host genes). Recently, recombinant adenoviruses have been used to deliver RNAi into cells.
        Two methods have traditionally been used to generate recombinant adenoviruses. The first involves homologous recombination of a shuttle vector containing gene of interest and an adenoviral backbone plasmid vector (restricted in E1/E3) in an adenovirus packaging cell line. The isolation of recombinant adenovirus by this method involves performing multiple plaque isolations to avoid wild-type virus and is extremely laborious and time consuming. The second method, pAdEasy system, employs the homologous recombination machinery in E. coli, a recombinant adenovirus is produced by a doublerecombination event between cotransformed adenoviral backbone plasmid vector and a shuttle vector carrying the gene of interest. For the pAdEasy method, the system is high fidelity, but inefficient and requires the screening of many bacterial colonies. This results in a significant time commitment even before transfection of recombinant DNA into E1-expressing cells such as HEK293 cells. 

质粒图谱

质粒序列

LOCUS       Exported               34947 bp ds-DNA     circular SYN 05-NOV-2017
DEFINITION  synthetic circular DNA
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 34947)
            http://www.miaolingbio.com
FEATURES             Location/Qualifiers
     source          1..34947
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     polyA_signal    34..168
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    169..32952
                     /label=AD
     misc_feature    4172..4275
                     /label=Ad SA
                     /note="adenovirus major late transcript splice acceptor 
                     (Friedrich and Soriano, 1991)"
     repeat_region   32850..32952
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     rep_origin      complement(33143..33731)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(33882..34742)
                     /label=Amp
     promoter        complement(34743..34847)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ttaattaagc tagagcggcc gccaccgcgg ggagatccag acatgataag atacattgat
       61 gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt
      121 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat
      181 tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta aagcaagtaa
      241 aacctctaca aatgtggtat ggctgattat gatccggctg cctcgcgcgt ttcggtgatg
      301 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg
      361 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg
      421 cagccatgag gtcgactcta gtccccgcgg tggcagatct ggaaggtgct gaggtacgat
      481 gagacccgca ccaggtgcag accctgcgag tgtggcggta aacatattag gaaccagcct
      541 gtgatgctgg atgtgaccga ggagctgagg cccgatcact tggtgctggc ctgcacccgc
      601 gctgagtttg gctctagcga tgaagataca gattgaggta ctgaaatgtg tgggcgtggc
      661 ttaagggtgg gaaagaatat ataaggtggg ggtcttatgt agttttgtat ctgttttgca
      721 gcagccgccg ccgccatgag caccaactcg tttgatggaa gcattgtgag ctcatatttg
      781 acaacgcgca tgcccccatg ggccggggtg cgtcagaatg tgatgggctc cagcattgat
      841 ggtcgccccg tcctgcccgc aaactctact accttgacct acgagaccgt gtctggaacg
      901 ccgttggaga ctgcagcctc cgccgccgct tcagccgctg cagccaccgc ccgcgggatt
      961 gtgactgact ttgctttcct gagcccgctt gcaagcagtg cagcttcccg ttcatccgcc
     1021 cgcgatgaca agttgacggc tcttttggca caattggatt ctttgacccg ggaacttaat
     1081 gtcgtttctc agcagctgtt ggatctgcgc cagcaggttt ctgccctgaa ggcttcctcc
     1141 cctcccaatg cggtttaaaa cataaataaa aaaccagact ctgtttggat ttggatcaag
     1201 caagtgtctt gctgtcttta tttaggggtt ttgcgcgcgc ggtaggcccg ggaccagcgg
     1261 tctcggtcgt tgagggtcct gtgtattttt tccaggacgt ggtaaaggtg actctggatg
     1321 ttcagataca tgggcataag cccgtctctg gggtggaggt agcaccactg cagagcttca
     1381 tgctgcgggg tggtgttgta gatgatccag tcgtagcagg agcgctgggc gtggtgccta
     1441 aaaatgtctt tcagtagcaa gctgattgcc aggggcaggc ccttggtgta agtgtttaca
     1501 aagcggttaa gctgggatgg gtgcatacgt ggggatatga gatgcatctt ggactgtatt
     1561 tttaggttgg ctatgttccc agccatatcc ctccggggat tcatgttgtg cagaaccacc
     1621 agcacagtgt atccggtgca cttgggaaat ttgtcatgta gcttagaagg aaatgcgtgg
     1681 aagaacttgg agacgccctt gtgacctcca agattttcca tgcattcgtc cataatgatg
     1741 gcaatgggcc cacgggcggc ggcctgggcg aagatatttc tgggatcact aacgtcatag
     1801 ttgtgttcca ggatgagatc gtcataggcc atttttacaa agcgcgggcg gagggtgcca
     1861 gactgcggta taatggttcc atccggccca ggggcgtagt taccctcaca gatttgcatt
     1921 tcccacgctt tgagttcaga tggggggatc atgtctacct gcggggcgat gaagaaaacg
     1981 gtttccgggg taggggagat cagctgggaa gaaagcaggt tcctgagcag ctgcgactta
     2041 ccgcagccgg tgggcccgta aatcacacct attaccggct gcaactggta gttaagagag
     2101 ctgcagctgc cgtcatccct gagcaggggg gccacttcgt taagcatgtc cctgactcgc
     2161 atgttttccc tgaccaaatc cgccagaagg cgctcgccgc ccagcgatag cagttcttgc
     2221 aaggaagcaa agtttttcaa cggtttgaga ccgtccgccg taggcatgct tttgagcgtt
     2281 tgaccaagca gttccaggcg gtcccacagc tcggtcacct gctctacggc atctcgatcc
     2341 agcatatctc ctcgtttcgc gggttggggc ggctttcgct gtacggcagt agtcggtgct
     2401 cgtccagacg ggccagggtc atgtctttcc acgggcgcag ggtcctcgtc agcgtagtct
     2461 gggtcacggt gaaggggtgc gctccgggct gcgcgctggc cagggtgcgc ttgaggctgg
     2521 tcctgctggt gctgaagcgc tgccggtctt cgccctgcgc gtcggccagg tagcatttga
     2581 ccatggtgtc atagtccagc ccctccgcgg cgtggccctt ggcgcgcagc ttgcccttgg
     2641 aggaggcgcc gcacgagggg cagtgcagac ttttgagggc gtagagcttg ggcgcgagaa
     2701 ataccgattc cggggagtag gcatccgcgc cgcaggcccc gcagacggtc tcgcattcca
     2761 cgagccaggt gagctctggc cgttcggggt caaaaaccag gtttccccca tgctttttga
     2821 tgcgtttctt acctctggtt tccatgagcc ggtgtccacg ctcggtgacg aaaaggctgt
     2881 ccgtgtcccc gtatacagac ttgagaggcc tgtcctcgag cggtgttccg cggtcctcct
     2941 cgtatagaaa ctcggaccac tctgagacaa aggctcgcgt ccaggccagc acgaaggagg
     3001 ctaagtggga ggggtagcgg tcgttgtcca ctagggggtc cactcgctcc agggtgtgaa
     3061 gacacatgtc gccctcttcg gcatcaagga aggtgattgg tttgtaggtg taggccacgt
     3121 gaccgggtgt tcctgaaggg gggctataaa agggggtggg ggcgcgttcg tcctcactct
     3181 cttccgcatc gctgtctgcg agggccagct gttggggtga gtactccctc tgaaaagcgg
     3241 gcatgacttc tgcgctaaga ttgtcagttt ccaaaaacga ggaggatttg atattcacct
     3301 ggcccgcggt gatgcctttg agggtggccg catccatctg gtcagaaaag acaatctttt
     3361 tgttgtcaag cttggtggca aacgacccgt agagggcgtt ggacagcaac ttggcgatgg
     3421 agcgcagggt ttggtttttg tcgcgatcgg cgcgctcctt ggccgcgatg tttagctgca
     3481 cgtattcgcg cgcaacgcac cgccattcgg gaaagacggt ggtgcgctcg tcgggcacca
     3541 ggtgcacgcg ccaaccgcgg ttgtgcaggg tgacaaggtc aacgctggtg gctacctctc
     3601 cgcgtaggcg ctcgttggtc cagcagaggc ggccgccctt gcgcgagcag aatggcggta
     3661 gggggtctag ctgcgtctcg tccggggggt ctgcgtccac ggtaaagacc ccgggcagca
     3721 ggcgcgcgtc gaagtagtct atcttgcatc cttgcaagtc tagcgcctgc tgccatgcgc
     3781 gggcggcaag cgcgcgctcg tatgggttga gtgggggacc ccatggcatg gggtgggtga
     3841 gcgcggaggc gtacatgccg caaatgtcgt aaacgtagag gggctctctg agtattccaa
     3901 gatatgtagg gtagcatctt ccaccgcgga tgctggcgcg cacgtaatcg tatagttcgt
     3961 gcgagggagc gaggaggtcg ggaccgaggt tgctacgggc gggctgctct gctcggaaga
     4021 ctatctgcct gaagatggca tgtgagttgg atgatatggt tggacgctgg aagacgttga
     4081 agctggcgtc tgtgagacct accgcgtcac gcacgaagga ggcgtaggag tcgcgcagct
     4141 tgttgaccag ctcggcggtg acctgcacgt ctagggcgca gtagtccagg gtttccttga
     4201 tgatgtcata cttatcctgt cccttttttt tccacagctc gcggttgagg acaaactctt
     4261 cgcggtcttt ccagtactct tggatcggaa acccgtcggc ctccgaacgg taagagccta
     4321 gcatgtagaa ctggttgacg gcctggtagg cgcagcatcc cttttctacg ggtagcgcgt
     4381 atgcctgcgc ggccttccgg agcgaggtgt gggtgagcgc aaaggtgtcc ctgaccatga
     4441 ctttgaggta ctggtatttg aagtcagtgt cgtcgcatcc gccctgctcc cagagcaaaa
     4501 agtccgtgcg ctttttggaa cgcggatttg gcagggcgaa ggtgacatcg ttgaagagta
     4561 tctttcccgc gcgaggcata aagttgcgtg tgatgcggaa gggtcccggc acctcggaac
     4621 ggttgttaat tacctgggcg gcgagcacga tctcgtcaaa gccgttgatg ttgtggccca
     4681 caatgtaaag ttccaagaag cgcgggatgc ccttgatgga aggcaatttt ttaagttcct
     4741 cgtaggtgag ctcttcaggg gagctgagcc cgtgctctga aagggcccag tctgcaagat
     4801 gagggttgga agcgacgaat gagctccaca ggtcacgggc cattagcatt tgcaggtggt
     4861 cgcgaaaggt cctaaactgg cgacctatgg ccattttttc tggggtgatg cagtagaagg
     4921 taagcgggtc ttgttcccag cggtcccatc caaggttcgc ggctaggtct cgcgcggcag
     4981 tcactagagg ctcatctccg ccgaacttca tgaccagcat gaagggcacg agctgcttcc
     5041 caaaggcccc catccaagta taggtctcta catcgtaggt gacaaagaga cgctcggtgc
     5101 gaggatgcga gccgatcggg aagaactgga tctcccgcca ccaattggag gagtggctat
     5161 tgatgtggtg aaagtagaag tccctgcgac gggccgaaca ctcgtgctgg cttttgtaaa
     5221 aacgtgcgca gtactggcag cggtgcacgg gctgtacatc ctgcacgagg ttgacctgac
     5281 gaccgcgcac aaggaagcag agtgggaatt tgagcccctc gcctggcggg tttggctggt
     5341 ggtcttctac ttcggctgct tgtccttgac cgtctggctg ctcgagggga gttacggtgg
     5401 atcggaccac cacgccgcgc gagcccaaag tccagatgtc cgcgcgcggc ggtcggagct
     5461 tgatgacaac atcgcgcaga tgggagctgt ccatggtctg gagctcccgc ggcgtcaggt
     5521 caggcgggag ctcctgcagg tttacctcgc atagacgggt cagggcgcgg gctagatcca
     5581 ggtgatacct aatttccagg ggctggttgg tggcggcgtc gatggcttgc aagaggccgc
     5641 atccccgcgg cgcgactacg gtaccgcgcg gcgggcggtg ggccgcgggg gtgtccttgg
     5701 atgatgcatc taaaagcggt gacgcgggcg agcccccgga ggtagggggg gctccggacc
     5761 cgccgggaga gggggcaggg gcacgtcggc gccgcgcgcg ggcaggagct ggtgctgcgc
     5821 gcgtaggttg ctggcgaacg cgacgacgcg gcggttgatc tcctgaatct ggcgcctctg
     5881 cgtgaagacg acgggcccgg tgagcttgaa cctgaaagag agttcgacag aatcaatttc
     5941 ggtgtcgttg acggcggcct ggcgcaaaat ctcctgcacg tctcctgagt tgtcttgata
     6001 ggcgatctcg gccatgaact gctcgatctc ttcctcctgg agatctccgc gtccggctcg
     6061 ctccacggtg gcggcgaggt cgttggaaat gcgggccatg agctgcgaga aggcgttgag
     6121 gcctccctcg ttccagacgc ggctgtagac cacgccccct tcggcatcgc gggcgcgcat
     6181 gaccacctgc gcgagattga gctccacgtg ccgggcgaag acggcgtagt ttcgcaggcg
     6241 ctgaaagagg tagttgaggg tggtggcggt gtgttctgcc acgaagaagt acataaccca
     6301 gcgtcgcaac gtggattcgt tgatatcccc caaggcctca aggcgctcca tggcctcgta
     6361 gaagtccacg gcgaagttga aaaactggga gttgcgcgcc gacacggtta actcctcctc
     6421 cagaagacgg atgagctcgg cgacagtgtc gcgcacctcg cgctcaaagg ctacaggggc
     6481 ctcttcttct tcttcaatct cctcttccat aagggcctcc ccttcttctt cttctggcgg
     6541 cggtggggga ggggggacac ggcggcgacg acggcgcacc gggaggcggt cgacaaagcg
     6601 ctcgatcatc tccccgcggc gacggcgcat ggtctcggtg acggcgcggc cgttctcgcg
     6661 ggggcgcagt tggaagacgc cgcccgtcat gtcccggtta tgggttggcg gggggctgcc
     6721 atgcggcagg gatacggcgc taacgatgca tctcaacaat tgttgtgtag gtactccgcc
     6781 gccgagggac ctgagcgagt ccgcatcgac cggatcggaa aacctctcga gaaaggcgtc
     6841 taaccagtca cagtcgcaag gtaggctgag caccgtggcg ggcggcagcg ggcggcggtc
     6901 ggggttgttt ctggcggagg tgctgctgat gatgtaatta aagtaggcgg tcttgagacg
     6961 gcggatggtc gacagaagca ccatgtcctt gggtccggcc tgctgaatgc gcaggcggtc
     7021 ggccatgccc caggcttcgt tttgacatcg gcgcaggtct ttgtagtagt cttgcatgag
     7081 cctttctacc ggcacttctt cttctccttc ctcttgtcct gcatctcttg catctatcgc
     7141 tgcggcggcg gcggagtttg gccgtaggtg gcgccctctt cctcccatgc gtgtgacccc
     7201 gaagcccctc atcggctgaa gcagggctag gtcggcgaca acgcgctcgg ctaatatggc
     7261 ctgctgcacc tgcgtgaggg tagactggaa gtcatccatg tccacaaagc ggtggtatgc
     7321 gcccgtgttg atggtgtaag tgcagttggc cataacggac cagttaacgg tctggtgacc
     7381 cggctgcgag agctcggtgt acctgagacg cgagtaagcc ctcgagtcaa atacgtagtc
     7441 gttgcaagtc cgcaccaggt actggtatcc caccaaaaag tgcggcggcg gctggcggta
     7501 gaggggccag cgtagggtgg ccggggctcc gggggcgaga tcttccaaca taaggcgatg
     7561 atatccgtag atgtacctgg acatccaggt gatgccggcg gcggtggtgg aggcgcgcgg
     7621 aaagtcgcgg acgcggttcc agatgttgcg cagcggcaaa aagtgctcca tggtcgggac
     7681 gctctggccg gtcaggcgcg cgcaatcgtt gacgctctag cgtgcaaaag gagagcctgt
     7741 aagcgggcac tcttccgtgg tctggtggat aaattcgcaa gggtatcatg gcggacgacc
     7801 ggggttcgag ccccgtatcc ggccgtccgc cgtgatccat gcggttaccg cccgcgtgtc
     7861 gaacccaggt gtgcgacgtc agacaacggg ggagtgctcc ttttggcttc cttccaggcg
     7921 cggcggctgc tgcgctagct tttttggcca ctggccgcgc gcagcgtaag cggttaggct
     7981 ggaaagcgaa agcattaagt ggctcgctcc ctgtagccgg agggttattt tccaagggtt
     8041 gagtcgcggg acccccggtt cgagtctcgg accggccgga ctgcggcgaa cgggggtttg
     8101 cctccccgtc atgcaagacc ccgcttgcaa attcctccgg aaacagggac gagccccttt
     8161 tttgcttttc ccagatgcat ccggtgctgc ggcagatgcg cccccctcct cagcagcggc
     8221 aagagcaaga gcagcggcag acatgcaggg caccctcccc tcctcctacc gcgtcaggag
     8281 gggcgacatc cgcggttgac gcggcagcag atggtgatta cgaacccccg cggcgccggg
     8341 cccggcacta cctggacttg gaggagggcg agggcctggc gcggctagga gcgccctctc
     8401 ctgagcggca cccaagggtg cagctgaagc gtgatacgcg tgaggcgtac gtgccgcggc
     8461 agaacctgtt tcgcgaccgc gagggagagg agcccgagga gatgcgggat cgaaagttcc
     8521 acgcagggcg cgagctgcgg catggcctga atcgcgagcg gttgctgcgc gaggaggact
     8581 ttgagcccga cgcgcgaacc gggattagtc ccgcgcgcgc acacgtggcg gccgccgacc
     8641 tggtaaccgc atacgagcag acggtgaacc aggagattaa ctttcaaaaa agctttaaca
     8701 accacgtgcg tacgcttgtg gcgcgcgagg aggtggctat aggactgatg catctgtggg
     8761 actttgtaag cgcgctggag caaaacccaa atagcaagcc gctcatggcg cagctgttcc
     8821 ttatagtgca gcacagcagg gacaacgagg cattcaggga tgcgctgcta aacatagtag
     8881 agcccgaggg ccgctggctg ctcgatttga taaacatcct gcagagcata gtggtgcagg
     8941 agcgcagctt gagcctggct gacaaggtgg ccgccatcaa ctattccatg cttagcctgg
     9001 gcaagtttta cgcccgcaag atataccata ccccttacgt tcccatagac aaggaggtaa
     9061 agatcgaggg gttctacatg cgcatggcgc tgaaggtgct taccttgagc gacgacctgg
     9121 gcgtttatcg caacgagcgc atccacaagg ccgtgagcgt gagccggcgg cgcgagctca
     9181 gcgaccgcga gctgatgcac agcctgcaaa gggccctggc tggcacgggc agcggcgata
     9241 gagaggccga gtcctacttt gacgcgggcg ctgacctgcg ctgggcccca agccgacgcg
     9301 ccctggaggc agctggggcc ggacctgggc tggcggtggc acccgcgcgc gctggcaacg
     9361 tcggcggcgt ggaggaatat gacgaggacg atgagtacga gccagaggac ggcgagtact
     9421 aagcggtgat gtttctgatc agatgatgca agacgcaacg gacccggcgg tgcgggcggc
     9481 gctgcagagc cagccgtccg gccttaactc cacggacgac tggcgccagg tcatggaccg
     9541 catcatgtcg ctgactgcgc gcaatcctga cgcgttccgg cagcagccgc aggccaaccg
     9601 gctctccgca attctggaag cggtggtccc ggcgcgcgca aaccccacgc acgagaaggt
     9661 gctggcgatc gtaaacgcgc tggccgaaaa cagggccatc cggcccgacg aggccggcct
     9721 ggtctacgac gcgctgcttc agcgcgtggc tcgttacaac agcggcaacg tgcagaccaa
     9781 cctggaccgg ctggtggggg atgtgcgcga ggccgtggcg cagcgtgagc gcgcgcagca
     9841 gcagggcaac ctgggctcca tggttgcact aaacgccttc ctgagtacac agcccgccaa
     9901 cgtgccgcgg ggacaggagg actacaccaa ctttgtgagc gcactgcggc taatggtgac
     9961 tgagacaccg caaagtgagg tgtaccagtc tgggccagac tattttttcc agaccagtag
    10021 acaaggcctg cagaccgtaa acctgagcca ggctttcaaa aacttgcagg ggctgtgggg
    10081 ggtgcgggct cccacaggcg accgcgcgac cgtgtctagc ttgctgacgc ccaactcgcg
    10141 cctgttgctg ctgctaatag cgcccttcac ggacagtggc agcgtgtccc gggacacata
    10201 cctaggtcac ttgctgacac tgtaccgcga ggccataggt caggcgcatg tggacgagca
    10261 tactttccag gagattacaa gtgtcagccg cgcgctgggg caggaggaca cgggcagcct
    10321 ggaggcaacc ctaaactacc tgctgaccaa ccggcggcag aagatcccct cgttgcacag
    10381 tttaaacagc gaggaggagc gcattttgcg ctacgtgcag cagagcgtga gccttaacct
    10441 gatgcgcgac ggggtaacgc ccagcgtggc gctggacatg accgcgcgca acatggaacc
    10501 gggcatgtat gcctcaaacc ggccgtttat caaccgccta atggactact tgcatcgcgc
    10561 ggccgccgtg aaccccgagt atttcaccaa tgccatcttg aacccgcact ggctaccgcc
    10621 ccctggtttc tacaccgggg gattcgaggt gcccgagggt aacgatggat tcctctggga
    10681 cgacatagac gacagcgtgt tttccccgca accgcagacc ctgctagagt tgcaacagcg
    10741 cgagcaggca gaggcggcgc tgcgaaagga aagcttccgc aggccaagca gcttgtccga
    10801 tctaggcgct gcggccccgc ggtcagatgc tagtagccca tttccaagct tgatagggtc
    10861 tcttaccagc actcgcacca cccgcccgcg cctgctgggc gaggaggagt acctaaacaa
    10921 ctcgctgctg cagccgcagc gcgaaaaaaa cctgcctccg gcatttccca acaacgggat
    10981 agagagccta gtggacaaga tgagtagatg gaagacgtac gcgcaggagc acagggacgt
    11041 gccaggcccg cgcccgccca cccgtcgtca aaggcacgac cgtcagcggg gtctggtgtg
    11101 ggaggacgat gactcggcag acgacagcag cgtcctggat ttgggaggga gtggcaaccc
    11161 gtttgcgcac cttcgcccca ggctggggag aatgttttaa aaaaaaaaaa agcatgatgc
    11221 aaaataaaaa actcaccaag gccatggcac cgagcgttgg ttttcttgta ttccccttag
    11281 tatgcggcgc gcggcgatgt atgaggaagg tcctcctccc tcctacgaga gtgtggtgag
    11341 cgcggcgcca gtggcggcgg cgctgggttc tcccttcgat gctcccctgg acccgccgtt
    11401 tgtgcctccg cggtacctgc ggcctaccgg ggggagaaac agcatccgtt actctgagtt
    11461 ggcaccccta ttcgacacca cccgtgtgta cctggtggac aacaagtcaa cggatgtggc
    11521 atccctgaac taccagaacg accacagcaa ctttctgacc acggtcattc aaaacaatga
    11581 ctacagcccg ggggaggcaa gcacacagac catcaatctt gacgaccggt cgcactgggg
    11641 cggcgacctg aaaaccatcc tgcataccaa catgccaaat gtgaacgagt tcatgtttac
    11701 caataagttt aaggcgcggg tgatggtgtc gcgcttgcct actaaggaca atcagcgtgg
    11761 agctgaaata cagagtgggt ggagttcacg ctgcccgagg gcaactactc cgagaccatg
    11821 accatagacc ttatgaacaa cgcgatcgtg gagcactact tgaaagtggg cagacagaac
    11881 ggggttctgg aaagcgacat cggggtaaag tttgacaccc gcaacttcag actggggttt
    11941 gaccccgtca ctggtcttgt catgcctggg gtatatacaa acgaagcctt ccatccagac
    12001 atcattttgc tgccaggatg cggggtggac ttcacccaca gccgcctgag caacttgttg
    12061 ggcatccgca agcggcaacc cttccaggag ggctttagga tcacctacga tgatctggag
    12121 ggtggtaaca ttcccgcact gttggatgtg gacgcctacc aggcgagctt gaaagatgac
    12181 accgaacagg gcgggggtgg cgcaggcggc agcaacagca gtggcagcgg cgcggaagag
    12241 aactccaacg cggcagccgc ggcaatgcag ccggtggagg acatgaacga tcatgccatt
    12301 cgcggcgaca cctttgccac acgggctgag gagaagcgcg ctgaggccga agcagcggcc
    12361 gaagctgccg cccccgctgc gcaacccgag gtcgagaagc ctcagaagaa accggtgatc
    12421 aaacccctga cagaggacag caagaaacgc agttacaacc taataagcaa tgacagcacc
    12481 ttcacccagt accgcagctg gtaccttgca tacaactacg gcgaccctca gaccggaatc
    12541 cgctcatgga ccctgctttg cactcctgac gtaacctgcg gctcggagca ggtctactgg
    12601 tcgttgccag acatgatgca agaccccgtg accttccgct ccacgcgcca gatcagcaac
    12661 tttccggtgg tgggcgccga gctgttgccc gtgcactcca agagcttcta caacgaccag
    12721 gccgtctact cccaactcat ccgccagttt acctctctga cccacgtgtt caatcgcttt
    12781 cccgagaacc agattttggc gcgcccgcca gcccccacca tcaccaccgt cagtgaaaac
    12841 gttcctgctc tcacagatca cgggacgcta ccgctgcgca acagcatcgg aggagtccag
    12901 cgagtgacca ttactgacgc cagacgccgc acctgcccct acgtttacaa ggccctgggc
    12961 atagtctcgc cgcgcgtcct atcgagccgc actttttgag caagcatgtc catccttata
    13021 tcgcccagca ataacacagg ctggggcctg cgcttcccaa gcaagatgtt tggcggggcc
    13081 aagaagcgct ccgaccaaca cccagtgcgc gtgcgcgggc actaccgcgc gccctggggc
    13141 gcgcacaaac gcggccgcac tgggcgcacc accgtcgatg acgccatcga cgcggtggtg
    13201 gaggaggcgc gcaactacac gcccacgccg ccaccagtgt ccacagtgga cgcggccatt
    13261 cagaccgtgg tgcgcggagc ccggcgctat gctaaaatga agagacggcg gaggcgcgta
    13321 gcacgtcgcc accgccgccg acccggcact gccgcccaac gcgcggcggc ggccctgctt
    13381 aaccgcgcac gtcgcaccgg ccgacgggcg gccatgcggg ccgctcgaag gctggccgcg
    13441 ggtattgtca ctgtgccccc caggtccagg cgacgagcgg ccgccgcagc agccgcggcc
    13501 attagtgcta tgactcaggg tcgcaggggc aacgtgtatt gggtgcgcga ctcggttagc
    13561 ggcctgcgcg tgcccgtgcg cacccgcccc ccgcgcaact agattgcaag aaaaaactac
    13621 ttagactcgt actgttgtat gtatccagcg gcggcggcgc gcaacgaagc tatgtccaag
    13681 cgcaaaatca aagaagagat gctccaggtc atcgcgccgg agatctatgg ccccccgaag
    13741 aaggaagagc aggattacaa gccccgaaag ctaaagcggg tcaaaaagaa aaagaaagat
    13801 gatgatgatg aacttgacga cgaggtggaa ctgctgcacg ctaccgcgcc caggcgacgg
    13861 gtacagtgga aaggtcgacg cgtaaaacgt gttttgcgac ccggcaccac cgtagtcttt
    13921 acgcccggtg agcgctccac ccgcacctac aagcgcgtgt atgatgaggt gtacggcgac
    13981 gaggacctgc ttgagcaggc caacgagcgc ctcggggagt ttgcctacgg aaagcggcat
    14041 aaggacatgc tggcgttgcc gctggacgag ggcaacccaa cacctagcct aaagcccgta
    14101 acactgcagc aggtgctgcc cgcgcttgca ccgtccgaag aaaagcgcgg cctaaagcgc
    14161 gagtctggtg acttggcacc caccgtgcag ctgatggtac ccaagcgcca gcgactggaa
    14221 gatgtcttgg aaaaaatgac cgtggaacct gggctggagc ccgaggtccg cgtgcggcca
    14281 atcaagcagg tggcgccggg actgggcgtg cagaccgtgg acgttcagat acccactacc
    14341 agtagcacca gtattgccac cgccacagag ggcatggaga cacaaacgtc cccggttgcc
    14401 tcagcggtgg cggatgccgc ggtgcaggcg gtcgctgcgg ccgcgtccaa gacctctacg
    14461 gaggtgcaaa cggacccgtg gatgtttcgc gtttcagccc cccggcgccc gcgccgttcg
    14521 aggaagtacg gcgccgccag cgcgctactg cccgaatatg ccctacatcc ttccattgcg
    14581 cctacccccg gctatcgtgg ctacacctac cgccccagaa gacgagcaac tacccgacgc
    14641 cgaaccacca ctggaacccg ccgccgccgt cgccgtcgcc agcccgtgct ggccccgatt
    14701 tccgtgcgca gggtggctcg cgaaggaggc aggaccctgg tgctgccaac agcgcgctac
    14761 caccccagca tcgtttaaaa gccggtcttt gtggttcttg cagatatggc cctcacctgc
    14821 cgcctccgtt tcccggtgcc gggattccga ggaagaatgc accgtaggag gggcatggcc
    14881 ggccacggcc tgacgggcgg catgcgtcgt gcgcaccacc ggcggcggcg cgcgtcgcac
    14941 cgtcgcatgc gcggcggtat cctgcccctc cttattccac tgatcgccgc ggcgattggc
    15001 gccgtgcccg gaattgcatc cgtggccttg caggcgcaga gacactgatt aaaaacaagt
    15061 tgcatgtgga aaaatcaaaa taaaaagtct ggactctcac gctcgcttgg tcctgtaact
    15121 attttgtaga atggaagaca tcaactttgc gtctctggcc ccgcgacacg gctcgcgccc
    15181 gttcatggga aactggcaag atatcggcac cagcaatatg agcggtggcg ccttcagctg
    15241 gggctcgctg tggagcggca ttaaaaattt cggttccacc gttaagaact atggcagcaa
    15301 ggcctggaac agcagcacag gccagatgct gagggataag ttgaaagagc aaaatttcca
    15361 acaaaaggtg gtagatggcc tggcctctgg cattagcggg gtggtggacc tggccaacca
    15421 ggcagtgcaa aataagatta acagtaagct tgatccccgc cctcccgtag aggagcctcc
    15481 accggccgtg gagacagtgt ctccagaggg gcgtggcgaa aagcgtccgc gccccgacag
    15541 ggaagaaact ctggtgacgc aaatagacga gcctccctcg tacgaggagg cactaaagca
    15601 aggcctgccc accacccgtc ccatcgcgcc catggctacc ggagtgctgg gccagcacac
    15661 acccgtaacg ctggacctgc ctccccccgc cgacacccag cagaaacctg tgctgccagg
    15721 cccgaccgcc gttgttgtaa cccgtcctag ccgcgcgtcc ctgcgccgcg ccgccagcgg
    15781 tccgcgatcg ttgcggcccg tagccagtgg caactggcaa agcacactga acagcatcgt
    15841 gggtctgggg gtgcaatccc tgaagcgccg acgatgcttc tgatagctaa cgtgtcgtat
    15901 gtgtgtcatg tatgcgtcca tgtcgccgcc agaggagctg ctgagccgcc gcgcgcccgc
    15961 tttccaagat ggctacccct tcgatgatgc cgcagtggtc ttacatgcac atctcgggcc
    16021 aggacgcctc ggagtacctg agccccgggc tggtgcagtt tgcccgcgcc accgagacgt
    16081 acttcagcct gaataacaag tttagaaacc ccacggtggc gcctacgcac gacgtgacca
    16141 cagaccggtc ccagcgtttg acgctgcggt tcatccctgt ggaccgtgag gatactgcgt
    16201 actcgtacaa ggcgcggttc accctagctg tgggtgataa ccgtgtgctg gacatggctt
    16261 ccacgtactt tgacatccgc ggcgtgctgg acaggggccc tacttttaag ccctactctg
    16321 gcactgccta caacgccctg gctcccaagg gtgccccaaa tccttgcgaa tgggatgaag
    16381 ctgctactgc tcttgaaata aacctagaag aagaggacga tgacaacgaa gacgaagtag
    16441 acgagcaagc tgagcagcaa aaaactcacg tatttgggca ggcgccttat tctggtataa
    16501 atattacaaa ggagggtatt caaataggtg tcgaaggtca aacacctaaa tatgccgata
    16561 aaacatttca acctgaacct caaataggag aatctcagtg gtacgaaaca gaaattaatc
    16621 atgcagctgg gagagtccta aaaaagacta ccccaatgaa accatgttac ggttcatatg
    16681 caaaacccac aaatgaaaat ggagggcaag gcattcttgt aaagcaacaa aatggaaagc
    16741 tagaaagtca agtggaaatg caatttttct caactactga ggcagccgca ggcaatggtg
    16801 ataacttgac tcctaaagtg gtattgtaca gtgaagatgt agatatagaa accccagaca
    16861 ctcatatttc ttacatgccc actattaagg aaggtaactc acgagaacta atgggccaac
    16921 aatctatgcc caacaggcct aattacattg cttttaggga caattttatt ggtctaatgt
    16981 attacaacag cacgggtaat atgggtgttc tggcgggcca agcatcgcag ttgaatgctg
    17041 ttgtagattt gcaagacaga aacacagagc tttcatacca gcttttgctt gattccattg
    17101 gtgatagaac caggtacttt tctatgtgga atcaggctgt tgacagctat gatccagatg
    17161 ttagaattat tgaaaatcat ggaactgaag atgaacttcc aaattactgc tttccactgg
    17221 gaggtgtgat taatacagag actcttacca aggtaaaacc taaaacaggt caggaaaatg
    17281 gatgggaaaa agatgctaca gaattttcag ataaaaatga aataagagtt ggaaataatt
    17341 ttgccatgga aatcaatcta aatgccaacc tgtggagaaa tttcctgtac tccaacatag
    17401 cgctgtattt gcccgacaag ctaaagtaca gtccttccaa cgtaaaaatt tctgataacc
    17461 caaacaccta cgactacatg aacaagcgag tggtggctcc cgggctagtg gactgctaca
    17521 ttaaccttgg agcacgctgg tcccttgact atatggacaa cgtcaaccca tttaaccacc
    17581 accgcaatgc tggcctgcgc taccgctcaa tgttgctggg caatggtcgc tatgtgccct
    17641 tccacatcca ggtgcctcag aagttctttg ccattaaaaa cctccttctc ctgccgggct
    17701 catacaccta cgagtggaac ttcaggaagg atgttaacat ggttctgcag agctccctag
    17761 gaaatgacct aagggttgac ggagccagca ttaagtttga tagcatttgc ctttacgcca
    17821 ccttcttccc catggcccac aacaccgcct ccacgcttga ggccatgctt agaaacgaca
    17881 ccaacgacca gtcctttaac gactatctct ccgccgccaa catgctctac cctatacccg
    17941 ccaacgctac caacgtgccc atatccatcc cctcccgcaa ctgggcggct ttccgcggct
    18001 gggccttcac gcgccttaag actaaggaaa ccccatcact gggctcgggc tacgaccctt
    18061 attacaccta ctctggctct ataccctacc tagatggaac cttttacctc aaccacacct
    18121 ttaagaaggt ggccattacc tttgactctt ctgtcagctg gcctggcaat gaccgcctgc
    18181 ttacccccaa cgagtttgaa attaagcgct cagttgacgg ggagggttac aacgttgccc
    18241 agtgtaacat gaccaaagac tggttcctgg tacaaatgct agctaactat aacattggct
    18301 accagggctt ctatatccca gagagctaca aggaccgcat gtactccttc tttagaaact
    18361 tccagcccat gagccgtcag gtggtggatg atactaaata caaggactac caacaggtgg
    18421 gcatcctaca ccaacacaac aactctggat ttgttggcta ccttgccccc accatgcgcg
    18481 aaggacaggc ctaccctgct aacttcccct atccgcttat aggcaagacc gcagttgaca
    18541 gcattaccca gaaaaagttt ctttgcgatc gcaccctttg gcgcatccca ttctccagta
    18601 actttatgtc catgggcgca ctcacagacc tgggccaaaa ccttctctac gccaactccg
    18661 cccacgcgct agacatgact tttgaggtgg atcccatgga cgagcccacc cttctttatg
    18721 ttttgtttga agtctttgac gtggtccgtg tgcaccagcc gcaccgcggc gtcatcgaaa
    18781 ccgtgtacct gcgcacgccc ttctcggccg gcaacgccac aacataaaga agcaagcaac
    18841 atcaacaaca gctgccgcca tgggctccag tgagcaggaa ctgaaagcca ttgtcaaaga
    18901 tcttggttgt gggccatatt ttttgggcac ctatgacaag cgctttccag gctttgtttc
    18961 tccacacaag ctcgcctgcg ccatagtcaa tacggccggt cgcgagactg ggggcgtaca
    19021 ctggatggcc tttgcctgga acccgcactc aaaaacatgc tacctctttg agccctttgg
    19081 cttttctgac cagcgactca agcaggttta ccagtttgag tacgagtcac tcctgcgccg
    19141 tagcgccatt gcttcttccc ccgaccgctg tataacgctg gaaaagtcca cccaaagcgt
    19201 acaggggccc aactcggccg cctgtggact attctgctgc atgtttctcc acgcctttgc
    19261 caactggccc caaactccca tggatcacaa ccccaccatg aaccttatta ccggggtacc
    19321 caactccatg ctcaacagtc cccaggtaca gcccaccctg cgtcgcaacc aggaacagct
    19381 ctacagcttc ctggagcgcc actcgcccta cttccgcagc cacagtgcgc agattaggag
    19441 cgccacttct ttttgtcact tgaaaaacat gtaaaaataa tgtactagag acactttcaa
    19501 taaaggcaaa tgcttttatt tgtacactct cgggtgatta tttaccccca cccttgccgt
    19561 ctgcgccgtt taaaaatcaa aggggttctg ccgcgcatcg ctatgcgcca ctggcaggga
    19621 cacgttgcga tactggtgtt tagtgctcca cttaaactca ggcacaacca tccgcggcag
    19681 ctcggtgaag ttttcactcc acaggctgcg caccatcacc aacgcgttta gcaggtcggg
    19741 cgccgatatc ttgaagtcgc agttggggcc tccgccctgc gcgcgcgagt tgcgatacac
    19801 agggttgcag cactggaaca ctatcagcgc cgggtggtgc acgctggcca gcacgctctt
    19861 gtcggagatc agatccgcgt ccaggtcctc cgcgttgctc agggcgaacg gagtcaactt
    19921 tggtagctgc cttcccaaaa agggcgcgtg cccaggcttt gagttgcact cgcaccgtag
    19981 tggcatcaaa aggtgaccgt gcccggtctg ggcgttagga tacagcgcct gcataaaagc
    20041 cttgatctgc ttaaaagcca cctgagcctt tgcgccttca gagaagaaca tgccgcaaga
    20101 cttgccggaa aactgattgg ccggacaggc cgcgtcgtgc acgcagcacc ttgcgtcggt
    20161 gttggagatc tgcaccacat ttcggcccca ccggttcttc acgatcttgg ccttgctaga
    20221 ctgctccttc agcgcgcgct gcccgttttc gctcgtcaca tccatttcaa tcacgtgctc
    20281 cttatttatc ataatgcttc cgtgtagaca cttaagctcg ccttcgatct cagcgcagcg
    20341 gtgcagccac aacgcgcagc ccgtgggctc gtgatgcttg taggtcacct ctgcaaacga
    20401 ctgcaggtac gcctgcagga atcgccccat catcgtcaca aaggtcttgt tgctggtgaa
    20461 ggtcagctgc aacccgcggt gctcctcgtt cagccaggtc ttgcatacgg ccgccagagc
    20521 ttccacttgg tcaggcagta gtttgaagtt cgcctttaga tcgttatcca cgtggtactt
    20581 gtccatcagc gcgcgcgcag cctccatgcc cttctcccac gcagacacga tcggcacact
    20641 cagcgggttc atcaccgtaa tttcactttc cgcttcgctg ggctcttcct cttcctcttg
    20701 cgtccgcata ccacgcgcca ctgggtcgtc ttcattcagc cgccgcactg tgcgcttacc
    20761 tcctttgcca tgcttgatta gcaccggtgg gttgctgaaa cccaccattt gtagcgccac
    20821 atcttctctt tcttcctcgc tgtccacgat tacctctggt gatggcgggc gctcgggctt
    20881 gggagaaggg cgcttctttt tcttcttggg cgcaatggcc aaatccgccg ccgaggtcga
    20941 tggccgcggg ctgggtgtgc gcggcaccag cgcgtcttgt gatgagtctt cctcgtcctc
    21001 ggactcgata cgccgcctca tccgcttttt tgggggcgcc cggggaggcg gcggcgacgg
    21061 ggacggggac gacacgtcct ccatggttgg gggacgtcgc gccgcaccgc gtccgcgctc
    21121 gggggtggtt tcgcgctgct cctcttcccg actggccatt tccttctcct ataggcagaa
    21181 aaagatcatg gagtcagtcg agaagaagga cagcctaacc gccccctctg agttcgccac
    21241 caccgcctcc accgatgccg ccaacgcgcc taccaccttc cccgtcgagg cacccccgct
    21301 tgaggaggag gaagtgatta tcgagcagga cccaggtttt gtaagcgaag acgacgagga
    21361 ccgctcagta ccaacagagg ataaaaagca agaccaggac aacgcagagg caaacgagga
    21421 acaagtcggg cggggggacg aaaggcatgg cgactaccta gatgtgggag acgacgtgct
    21481 gttgaagcat ctgcagcgcc agtgcgccat tatctgcgac gcgttgcaag agcgcagcga
    21541 tgtgcccctc gccatagcgg atgtcagcct tgcctacgaa cgccacctat tctcaccgcg
    21601 cgtacccccc aaacgccaag aaaacggcac atgcgagccc aacccgcgcc tcaacttcta
    21661 ccccgtattt gccgtgccag aggtgcttgc cacctatcac atctttttcc aaaactgcaa
    21721 gataccccta tcctgccgtg ccaaccgcag ccgagcggac aagcagctgg ccttgcggca
    21781 gggcgctgtc atacctgata tcgcctcgct caacgaagtg ccaaaaatct ttgagggtct
    21841 tggacgcgac gagaagcgcg cggcaaacgc tctgcaacag gaaaacagcg aaaatgaaag
    21901 tcactctgga gtgttggtgg aactcgaggg tgacaacgcg cgcctagccg tactaaaacg
    21961 cagcatcgag gtcacccact ttgcctaccc ggcacttaac ctacccccca aggtcatgag
    22021 cacagtcatg agtgagctga tcgtgcgccg tgcgcagccc ctggagaggg atgcaaattt
    22081 gcaagaacaa acagaggagg gcctacccgc agttggcgac gagcagctag cgcgctggct
    22141 tcaaacgcgc gagcctgccg acttggagga gcgacgcaaa ctaatgatgg ccgcagtgct
    22201 cgttaccgtg gagcttgagt gcatgcagcg gttctttgct gacccggaga tgcagcgcaa
    22261 gctagaggaa acattgcact acacctttcg acagggctac gtacgccagg cctgcaagat
    22321 ctccaacgtg gagctctgca acctggtctc ctaccttgga attttgcacg aaaaccgcct
    22381 tgggcaaaac gtgcttcatt ccacgctcaa gggcgaggcg cgccgcgact acgtccgcga
    22441 ctgcgtttac ttatttctat gctacacctg gcagacggcc atgggcgttt ggcagcagtg
    22501 cttggaggag tgcaacctca aggagctgca gaaactgcta aagcaaaact tgaaggacct
    22561 atggacggcc ttcaacgagc gctccgtggc cgcgcacctg gcggacatca ttttccccga
    22621 acgcctgctt aaaaccctgc aacagggtct gccagacttc accagtcaaa gcatgttgca
    22681 gaactttagg aactttatcc tagagcgctc aggaatcttg cccgccacct gctgtgcact
    22741 tcctagcgac tttgtgccca ttaagtaccg cgaatgccct ccgccgcttt ggggccactg
    22801 ctaccttctg cagctagcca actaccttgc ctaccactct gacataatgg aagacgtgag
    22861 cggtgacggt ctactggagt gtcactgtcg ctgcaaccta tgcaccccgc accgctccct
    22921 ggtttgcaat tcgcagctgc ttaacgaaag tcaaattatc ggtacctttg agctgcaggg
    22981 tccctcgcct gacgaaaagt ccgcggctcc ggggttgaaa ctcactccgg ggctgtggac
    23041 gtcggcttac cttcgcaaat ttgtacctga ggactaccac gcccacgaga ttaggttcta
    23101 cgaagaccaa tcccgcccgc ctaatgcgga gcttaccgcc tgcgtcatta cccagggcca
    23161 cattcttggc caattgcaag ccatcaacaa agcccgccaa gagtttctgc tacgaaaggg
    23221 acggggggtt tacttggacc cccagtccgg cgaggagctc aacccaatcc ccccgccgcc
    23281 gcagccctat cagcagcagc cgcgggccct tgcttcccag gatggcaccc aaaaagaagc
    23341 tgcagctgcc gccgccaccc acggacgagg aggaatactg ggacagtcag gcagaggagg
    23401 ttttggacga ggaggaggag gacatgatgg aagactggga gagcctagac gaggaagctt
    23461 ccgaggtcga agaggtgtca gacgaaacac cgtcaccctc ggtcgcattc ccctcgccgg
    23521 cgccccagaa atcggcaacc ggttccagca tggctacaac ctccgctcct caggcgccgc
    23581 cggcactgcc cgttcgccga cccaaccgta gatgggacac cactggaacc agggccggta
    23641 agtccaagca gccgccgccg ttagcccaag agcaacaaca gcgccaaggc taccgctcat
    23701 ggcgcgggca caagaacgcc atagttgctt gcttgcaaga ctgtgggggc aacatctcct
    23761 tcgcccgccg ctttcttctc taccatcacg gcgtggcctt cccccgtaac atcctgcatt
    23821 actaccgtca tctctacagc ccatactgca ccggcggcag cggcagcaac agcagcggcc
    23881 acacagaagc aaaggcgacc ggatagcaag actctgacaa agcccaagaa atccacagcg
    23941 gcggcagcag caggaggagg agcgctgcgt ctggcgccca acgaacccgt atcgacccgc
    24001 gagcttagaa acaggatttt tcccactctg tatgctatat ttcaacagag caggggccaa
    24061 gaacaagagc tgaaaataaa aaacaggtct ctgcgatccc tcacccgcag ctgcctgtat
    24121 cacaaaagcg aagatcagct tcggcgcacg ctggaagacg cggaggctct cttcagtaaa
    24181 tactgcgcgc tgactcttaa ggactagttt cgcgcccttt ctcaaattta agcgcgaaaa
    24241 ctacgtcatc tccagcggcc acacccggcg ccagcacctg ttgtcagcgc cattatgagc
    24301 aaggaaattc ccacgcccta catgtggagt taccagccac aaatgggact tgcggctgga
    24361 gctgcccaag actactcaac ccgaataaac tacatgagcg cgggacccca catgatatcc
    24421 cgggtcaacg gaatacgcgc ccaccgaaac cgaattctcc tggaacaggc ggctattacc
    24481 accacacctc gtaataacct taatccccgt agttggcccg ctgccctggt gtaccaggaa
    24541 agtcccgctc ccaccactgt ggtacttccc agagacgccc aggccgaagt tcagatgact
    24601 aactcagggg cgcagcttgc gggcggcttt cgtcacaggg tgcggtcgcc cgggcagggt
    24661 ataactcacc tgacaatcag agggcgaggt attcagctca acgacgagtc ggtgagctcc
    24721 tcgcttggtc tccgtccgga cgggacattt cagatcggcg gcgccggccg ctcttcattc
    24781 acgcctcgtc aggcaatcct aactctgcag acctcgtcct ctgagccgcg ctctggaggc
    24841 attggaactc tgcaatttat tgaggagttt gtgccatcgg tctactttaa ccccttctcg
    24901 ggacctcccg gccactatcc ggatcaattt attcctaact ttgacgcggt aaaggactcg
    24961 gcggacggct acgactgaat gttaagtgga gaggcagagc aactgcgcct gaaacacctg
    25021 gtccactgtc gccgccacaa gtgctttgcc cgcgactccg gtgagttttg ctactttgaa
    25081 ttgcccgagg atcatatcga gggcccggcg cacggcgtcc ggcttaccgc ccagggagag
    25141 cttgcccgta gcctgattcg ggagtttacc cagcgccccc tgctagttga gcgggacagg
    25201 ggaccctgtg ttctcactgt gatttgcaac tgtcctaacc ctggattaca tcaagatctt
    25261 tgttgccatc tctgtgctga gtataataaa tacagaaatt aaaatatact ggggctccta
    25321 tcgccatcct gtaaacgcca ccgtcttcac ccgcccaagc aaaccaaggc gaaccttacc
    25381 tggtactttt aacatctctc cctctgtgat ttacaacagt ttcaacccag acggagtgag
    25441 tctacgagag aacctctccg agctcagcta ctccatcaga aaaaacacca ccctccttac
    25501 ctgccgggaa cgtacgagtg cgtcaccggc cgctgcacca cacctaccgc ctgaccgtaa
    25561 accagacttt ttccggacag acctcaataa ctctgtttac cagaacagga ggtgagctta
    25621 gaaaaccctt agggtattag gccaaaggcg cagctactgt ggggtttatg aacaattcaa
    25681 gcaactctac gggctattct aattcaggtt tctctagggt tggggttatt ctctgtcttg
    25741 tgattctctt tattcttata ctaacgcttc tctgcctaag gctcgccgcc tgctgtgtgc
    25801 acatttgcat ttattgtcag ctttttaaac gctggggtcg ccacccaaga tgattaggta
    25861 cataatccta ggtttactca cccttgcgtc agcccacggt accacccaaa aggtggattt
    25921 taaggagcca gcctgtaatg ttacattcgc agctgaagct aatgagtgca ccactcttat
    25981 aaaatgcacc acagaacatg aaaagctgct tattcgccac aaaaacaaaa ttggcaagta
    26041 tgctgtttat gctatttggc agccaggtga cactacagag tataatgtta cagttttcca
    26101 gggtaaaagt cataaaactt ttatgtatac ttttccattt tatgaaatgt gcgacattac
    26161 catgtacatg agcaaacagt ataagttgtg gcccccacaa aattgtgtgg aaaacactgg
    26221 cactttctgc tgcactgcta tgctaattac agtgctcgct ttggtctgta ccctactcta
    26281 tattaaatac aaaagcagac gcagctttat tgaggaaaag aaaatgcctt aatttactaa
    26341 gttacaaagc taatgtcacc actaactgct ttactcgctg cttgcaaaac aaattcaaaa
    26401 agttagcatt ataattagaa taggatttaa accccccggt catttcctgc tcaataccat
    26461 tcccctgaac aattgactct atgtgggata tgctccagcg ctacaacctt gaagtcaggc
    26521 ttcctggatg tcagcatctg actttggcca gcacctgtcc cgcggatttg ttccagtcca
    26581 actacagcga cccaccctaa cagagatgac caacacaacc aacgcggccg ccgctaccgg
    26641 acttacatct accacaaata caccccaagt ttctgccttt gtcaataact gggataactt
    26701 gggcatgtgg tggttctcca tagcgcttat gtttgtatgc cttattatta tgtggctcat
    26761 ctgctgccta aagcgcaaac gcgcccgacc acccatctat agtcccatca ttgtgctaca
    26821 cccaaacaat gatggaatcc atagattgga cggactgaaa cacatgttct tttctcttac
    26881 agtatgatta aatgagacat gattcctcga gtttttatat tactgaccct tgttgcgctt
    26941 ttttgtgcgt gctccacatt ggctgcggtt tctcacatcg aagtagactg cattccagcc
    27001 ttcacagtct atttgcttta cggatttgtc accctcacgc tcatctgcag cctcatcact
    27061 gtggtcatcg cctttatcca gtgcattgac tgggtctgtg tgcgctttgc atatctcagc
    27121 tgctgccatg ttgtgttgct accatgttgt tttcatgtgt tgctgccatg ctcttgtcgc
    27181 cttagatctc tctttatgta gtgttgtggt gtctctcttg tcgtgatgtg tgttttgtcc
    27241 tatatatttt aatttttaat ccaaacccct gtccccgcag gaggcctttg gccttctggt
    27301 aggccgtcat tgaaaactga cttaactcgt taaattaaaa aaatgtaaaa aataatggtt
    27361 gagactcagc ccaacatcgg cagatgaggt ggattgagac tcagcccaac atcggcagat
    27421 gaggtggatt gagactcaac cccaacattg gcagatgagg tgaattagat gaggtggatt
    27481 gagactcatg agggtggtat gagggcccga cgtccacagg tgggagttgt gctttacagt
    27541 ccaacgtgca ggacgcttgg catttgccag agaacaccaa gattggcaaa ttcgcaactg
    27601 gcgccctgtg ctcttcacag acggaaaaat gaccaaaatc tgattatttt tgtaaaacgg
    27661 aaaccgaatg tccgacaaag ttcatttgat gacttcccgg taggtctggc cctgccgctg
    27721 ggcccgacgc cgtccgggaa ttttacaaac gatttcggac gtctagcatt cactcacctt
    27781 gtcaaggacc tgaggatctc tgcaccctta ttaagaccct gtgcggtctc aaagatctta
    27841 ttccctttaa ctaataaaaa aaaataataa agcatcactt acttaaaatc agttagcaaa
    27901 tttctgtcca gtttattcag cagcacctcc ttgccctcct cccagctctg gtattgcagc
    27961 ttcctcctgg ctgcaaactt tctccacaat ctaaatggaa tgtcagtttc ctcctgttcc
    28021 tgtccatccg cacccactat cttcatgttg ttgcagatga agcgcgcaag accgtctgaa
    28081 gataccttca accccgtgta tccatatgac acggaaaccg gtcctccaac tgtgcctttt
    28141 cttactcctc cctttgtatc ccccaatggg tttcaagaga gtccccctgg ggtactctct
    28201 ttgcgcctat ccgaacctct agttacctcc aatggcatgc ttgcgctcaa aatgggcaac
    28261 ggcctctctc tggacgaggc cggcaacctt acctcccaaa atgtaaccac tgtgagccca
    28321 cctctcaaaa aaaccaagtc aaacataaac ctggaaatat ctgcacccct cacagttacc
    28381 tcagaagccc taactgtggc tgccgccgca cctctaatgg tcgcgggcaa cacactcacc
    28441 atgcaatcac aggccccgct aaccgtgcac gactccaaac ttagcattgc cacccaagga
    28501 cccctcacag tgtcagaagg aaagctagcc ctgcaaacat caggccccct caccaccacc
    28561 gatagcagta cccttactat cactgcctca ccccctctaa ctactgccac tggtagcttg
    28621 ggcattgact tgaaagagcc catttataca caaaatggaa aactaggact aaagtacggg
    28681 gctcctttgc atgtaacaga cgacctaaac actttgaccg tagcaactgg tccaggtgtg
    28741 actattaata atacttcctt gcaaactaaa gttactggag ccttgggttt tgattcacaa
    28801 ggcaatatgc aacttaatgt agcaggagga ctaaggattg attctcaaaa cagacgcctt
    28861 atacttgatg ttagttatcc gtttgatgct caaaaccaac taaatctaag actaggacag
    28921 ggccctcttt ttataaactc agcccacaac ttggatatta actacaacaa aggcctttac
    28981 ttgtttacag cttcaaacaa ttccaaaaag cttgaggtta acctaagcac tgccaagggg
    29041 ttgatgtttg acgctacagc catagccatt aatgcaggag atgggcttga atttggttca
    29101 cctaatgcac caaacacaaa tcccctcaaa acaaaaattg gccatggcct agaatttgat
    29161 tcaaacaagg ctatggttcc taaactagga actggcctta gttttgacag cacaggtgcc
    29221 attacagtag gaaacaaaaa taatgataag ctaactttgt ggaccacacc agctccatct
    29281 cctaactgta gactaaatgc agagaaagat gctaaactca ctttggtctt aacaaaatgt
    29341 ggcagtcaaa tacttgctac agtttcagtt ttggctgtta aaggcagttt ggctccaata
    29401 tctggaacag ttcaaagtgc tcatcttatt ataagatttg acgaaaatgg agtgctacta
    29461 aacaattcct tcctggaccc agaatattgg aactttagaa atggagatct tactgaaggc
    29521 acagcctata caaacgctgt tggatttatg cctaacctat cagcttatcc aaaatctcac
    29581 ggtaaaactg ccaaaagtaa cattgtcagt caagtttact taaacggaga caaaactaaa
    29641 cctgtaacac taaccattac actaaacggt acacaggaaa caggagacac aactccaagt
    29701 gcatactcta tgtcattttc atgggactgg tctggccaca actacattaa tgaaatattt
    29761 gccacatcct cttacacttt ttcatacatt gcccaagaat aaagaatcgt ttgtgttatg
    29821 tttcaacgtg tttatttttc aattgcagaa aatttcaagt catttttcat tcagtagtat
    29881 agccccacca ccacatagct tatacagatc accgtacctt aatcaaactc acagaaccct
    29941 agtattcaac ctgccacctc cctcccaaca cacagagtac acagtccttt ctccccggct
    30001 ggccttaaaa agcatcatat catgggtaac agacatattc ttaggtgtta tattccacac
    30061 ggtttcctgt cgagccaaac gctcatcagt gatattaata aactccccgg gcagctcact
    30121 taagttcatg tcgctgtcca gctgctgagc cacaggctgc tgtccaactt gcggttgctt
    30181 aacgggcggc gaaggagaag tccacgccta catgggggta gagtcataat cgtgcatcag
    30241 gatagggcgg tggtgctgca gcagcggcga ataaactgct gccgccgccg ctccgtcctg
    30301 caggaataca acatggcagt ggtctcctca gcgatgattc gcaccgcccg cagcataagg
    30361 cgccttgtcc tccgggcaca gcagcgcacc ctgatctcac ttaaatcagc acagtaactg
    30421 cagcacagca ccacaatatt gttcaaaatc ccacagtgca aggcgctgta tccaaagctc
    30481 atggcgggga ccacagaacc cacgtggcca tcataccaca agcgcaggta gattaagtgg
    30541 cgacccctca taaacacgct ggacataaac attacctctt ttggcatgtt gtaattcacc
    30601 acctcccggt accatataaa cctctgatta aacatggcgc catccaccac catcctaaac
    30661 cagctggcca aaacctgccc gccggctata cactgcaggg aaccgggact ggaacaatga
    30721 cagtggagag cccaggactc gtaaccatgg atcatcatgc tcgtcatgat atcaatgttg
    30781 gcacaacaca ggcacacgtg catacacttc ctcaggatta caagctcctc ccgcgttaga
    30841 accatatccc agggaacaac ccattcctga atcagcgtaa atcccacact gcagggaaga
    30901 cctcgcacgt aactcacgtt gtgcattgtc aaagtgttac attcgggcag cagcggatga
    30961 tcctccagta tggtagcgcg ggtttctgtc tcaaaaggag gtagacgatc cctactgtac
    31021 ggagtgcgcc gagacaaccg agatcgtgtt ggtcgtagtg tcatgccaaa tggaacgccg
    31081 gacgtagtca tatttcctga agcaaaacca ggtgcgggcg tgacaaacag atctgcgtct
    31141 ccggtctcgc cgcttagatc gctctgtgta gtagttgtag tatatccact ctctcaaagc
    31201 atccaggcgc cccctggctt cgggttctat gtaaactcct tcatgcgccg ctgccctgat
    31261 aacatccacc accgcagaat aagccacacc cagccaacct acacattcgt tctgcgagtc
    31321 acacacggga ggagcgggaa gagctggaag aaccatgttt ttttttttat tccaaaagat
    31381 tatccaaaac ctcaaaatga agatctatta agtgaacgcg ctcccctccg gtggcgtggt
    31441 caaactctac agccaaagaa cagataatgg catttgtaag atgttgcaca atggcttcca
    31501 aaaggcaaac ggccctcacg tccaagtgga cgtaaaggct aaacccttca gggtgaatct
    31561 cctctataaa cattccagca ccttcaacca tgcccaaata attctcatct cgccaccttc
    31621 tcaatatatc tctaagcaaa tcccgaatat taagtccggc cattgtaaaa atctgctcca
    31681 gagcgccctc caccttcagc ctcaagcagc gaatcatgat tgcaaaaatt caggttcctc
    31741 acagacctgt ataagattca aaagcggaac attaacaaaa ataccgcgat cccgtaggtc
    31801 ccttcgcagg gccagctgaa cataatcgtg caggtctgca cggaccagcg cggccacttc
    31861 cccgccagga accatgacaa aagaacccac actgattatg acacgcatac tcggagctat
    31921 gctaaccagc gtagccccga tgtaagcttg ttgcatgggc ggcgatataa aatgcaaggt
    31981 gctgctcaaa aaatcaggca aagcctcgcg caaaaaagaa agcacatcgt agtcatgctc
    32041 atgcagataa aggcaggtaa gctccggaac caccacagaa aaagacacca tttttctctc
    32101 aaacatgtct gcgggtttct gcataaacac aaaataaaat aacaaaaaaa catttaaaca
    32161 ttagaagcct gtcttacaac aggaaaaaca acccttataa gcataagacg gactacggcc
    32221 atgccggcgt gaccgtaaaa aaactggtca ccgtgattaa aaagcaccac cgacagctcc
    32281 tcggtcatgt ccggagtcat aatgtaagac tcggtaaaca catcaggttg attcacatcg
    32341 gtcagtgcta aaaagcgacc gaaatagccc gggggaatac atacccgcag gcgtagagac
    32401 aacattacag cccccatagg aggtataaca aaattaatag gagagaaaaa cacataaaca
    32461 cctgaaaaac cctcctgcct aggcaaaata gcaccctccc gctccagaac aacatacagc
    32521 gcttccacag cggcagccat aacagtcagc cttaccagta aaaaagaaaa cctattaaaa
    32581 aaacaccact cgacacggca ccagctcaat cagtcacagt gtaaaaaagg gccaagtgca
    32641 gagcgagtat atataggact aaaaaatgac gtaacggtta aagtccacaa aaaacaccca
    32701 gaaaaccgca cgcgaaccta cgcccagaaa cgaaagccaa aaaacccaca acttcctcaa
    32761 atcgtcactt ccgttttccc acgttacgtc acttcccatt ttaagaaaac tacaattccc
    32821 aacacataca agttactccg ccctaaaacc tacgtcaccc gccccgttcc cacgccccgc
    32881 gccacgtcac aaactccacc ccctcattat catattggct tcaatccaaa ataaggtata
    32941 ttattgatga tgttaattaa gaattcggat ctgcgacgcg aggctggatg gccttcccca
    33001 ttatgattct tctcgcttcc ggcggcatcg ggatgcccgc gttgcaggcc atgctgtcca
    33061 ggcaggtaga tgacgaccat cagggacagc ttcacggcca gcaaaaggcc aggaaccgta
    33121 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
    33181 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
    33241 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
    33301 ccgcctttct cccttcggga agcgtggcgc tttctcaatg ctcacgctgt aggtatctca
    33361 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
    33421 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
    33481 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
    33541 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
    33601 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
    33661 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
    33721 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
    33781 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
    33841 taaatcaatc taaagtatat atgagtaaat tggtctgaca gttaccaatg cttaatcagt
    33901 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
    33961 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
    34021 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
    34081 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
    34141 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctgca
    34201 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
    34261 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
    34321 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
    34381 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
    34441 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca
    34501 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
    34561 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
    34621 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
    34681 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
    34741 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
    34801 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
    34861 aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
    34921 cgtatcacga ggccctttcg tcttcaa
//
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