LOCUS Exported 3276 bp ds-DNA circular SYN 28-SEP-2017
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3276)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Sep 28, 2017 from MiaoLingPlasmid
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..3276
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_feature complement(11..102)
/label=ITR
misc_signal 190..340
/label=Ad5 Psi
/note="packaging signal for adenovirus serotype 5"
misc_feature 455..502
/label=MCS
protein_bind 514..547
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
rep_origin complement(1010..1598)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(1769..2629)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(2630..2734)
/gene="bla"
/label=AmpR promoter
misc_feature 2817..453
/label=Ad
misc_feature 3186..1
/label=ITR
ORIGIN
1 ttcatcaata atatacctta ttttggattg aagccaatat gataatgagg gggtggagtt
61 tgtgacgtgg cgcggggcgt gggaacgggg cgggtgacgt agtagtgtgg cggaagtgtg
121 atgttgcaag tgtggcggaa cacatgtaag cgacggatgt ggcaaaagtg acgtttttgg
181 tgtgcgccgg tgtacacagg aagtgacaat tttcgcgcgg ttttaggcgg atgttgtagt
241 aaatttgggc gtaaccgagt aagatttggc cattttcgcg ggaaaactga ataagaggaa
301 gtgaaatctg aataattttg tgttactcat agcgcgtaat atttgtctag ggccgcgggg
361 actttgaccg tttacgtgga gactcgccca ggtgtttttc tcaggtgttt tccgcgttcc
421 gggtcaaagt tggcgtttta ttattatagt cagctctaga gaattcaggc ctgctagcag
481 atctaagctt gagctcgtcg actcgaagat ccaataactt cgtatagcat acattatacg
541 aagttataag tgccgcccta taccttgtct gcctccccgc gttgcgtcgc ggtgcatgga
601 gccgggccac ctcgacctga atggaagccg gcggcacctc gctaacggat tcaccactcc
661 aagaattgga gccaatcaat tcttgcggag aactgtgaat gcgcaaacca acccttggca
721 gaacatatcc atcgcgtccg ccatctccag cagccgcacg cggcgcatct cggctgcatt
781 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
841 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
901 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
961 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
1021 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
1081 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
1141 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
1201 ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
1261 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
1321 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
1381 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
1441 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
1501 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
1561 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
1621 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
1681 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
1741 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
1801 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
1861 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
1921 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
1981 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
2041 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
2101 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
2161 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
2221 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
2281 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
2341 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaacacgg gataataccg
2401 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
2461 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
2521 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
2581 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
2641 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
2701 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
2761 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcactctag
2821 gcaaaatagc accctcccgc tccagaacaa catacagcgc ttcacagcgg cagcctaaca
2881 gtcagcctta ccagtaaaaa agaaaaccta ttaaaaaaac accactcgac acggcaccag
2941 ctcaatcagt cacagtgtaa aaaagggcca agtgcagagc gagtatatat aggactaaaa
3001 aatgacgtaa cggttaaagt ccacaaaaaa cacccagaaa accgcacgcg aacctacgcc
3061 cagaaacgaa agccaaaaaa cccacaactt cctcaaatcg tcacttccgt tttcccacgt
3121 tacgtaactt cccattttaa gaaaactaca attcccaaca catacaagtt actccgccct
3181 aaaacctacg tcacccgccc cgttcccacg ccccgcgcca cgtcacaaac tccaccccct
3241 cattatcata ttggcttcaa tccaaaataa ggtata
//