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pAdEasy-1

pAdEasy-1
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  • 产品名称:pAdEasy-1
  • 产品型号:
  • 产品展商:XYbscience
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简单介绍
pAdEasy-1菌株发货前均经过严格的多重验证,如存在质量问题,请在收到产品的三个月内通知我司。pAdEasy-1收到质粒后请短暂离心,加入20μl ddH2O溶解质粒,取2μl转化至对应感受态中,挑取单克隆重新提取质粒后使用。
产品描述

pAdEasy-1

  • 产品信息
  • 基本信息
  • 质粒简介
  • 质粒图谱
  • 质粒序列

产品信息

产品货号 产品名称 产品规格 优惠价
XY0653 pAdEasy-1

20μl

¥请询价

使用说明

信裕质粒平台的各批次质粒菌株发货前均经过严格的多重验证,如存在质量问题,请在收到产品的三个月内通知我司。收到质粒后请短暂离心,加入20μl ddH2O溶解质粒,取2μl转化至对应感受态中,挑取单克隆重新提取质粒后使用。如需获取其他详细信息请登录我司官网查询。

基本信息

复制子: pUC ori
质粒分类: 病毒系列,腺病毒载体
质粒大小: 33477bp
原核抗性: Amp
克隆菌株: Stbl3
培养条件: 37℃,有氧 LB
表达宿主: 哺乳细胞
诱导方式: 无须诱导,瞬时表达
5'测序引物: M13F:TGTAAAACGACGGCCAGT
3'测序引物: 根据序列设计引物

质粒简介

      The plasmid pAdEasy-1, containing most of the human adenovirus serotype 5 (Ad5) genome, is deleted for the genes E1 and E3. The removal of these two viral genes creates space for foreign DNA and eliminates selfreplication capabilities. The E1 deletion renders the viruses defective for replication and incapable of producing infectious viral particles in target cells (provided there is no complementation by the host cell); the E3 region encodes proteins involved in evading host immunity and is dispensable. The deletion of both genes creates room for up to 7.5 kb of foreign DNA that can be inserted into the Ad5 genome. The E1 gene, which is necessary for production of viral particles, is provided in trans by AD-293 cells. pAdEasy-1 carries the ampicillin resistance gene, which is lost after recombination with a shuttle vector.

质粒图谱

质粒序列

LOCUS       Exported               33477 bp ds-DNA     circular SYN 06-SEP-2016
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    Untitled 6
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 33477)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, September 6, 2016 from SnapGene Viewer 3.1.4
            http://www.miaolingbio.com
FEATURES             Location/Qualifiers
     source          1..33477
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             1037..1228
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /note="rop"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     misc_feature    1330..1470
                     /note="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(1656..2244)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2415..3275)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3276..3380)
                     /gene="bla"
                     /note="AmpR promoter"
     misc_feature    6972..7075
                     /note="Ad SA"
                     /note="adenovirus major late transcript splice acceptor 
                     (Friedrich and Soriano, 1991)"
     CDS             complement(14168..14179)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /note="Factor Xa site"
                     /translation="IEGR"
     repeat_region   33121..33223
                     /note="ITR"
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
ORIGIN
        1 tcgtcactgg tcccgccacc aaacgtttcg gcgagaagca ggccattatc gccggcatgg
       61 cggccgacgc gctgggctac gtcttgctgg cgttcgcgac gcgaggctgg atggccttcc
      121 ccattatgat tcttctcgct tccggcggca tcgggatgcc cgcgttgcag gccatgctgt
      181 ccaggcaggt agatgacgac catcagggac agcttcaagg atcgctcgcg gctcttacca
      241 gcctaacttc gatcattgga ccgctgatcg tcacggcgat ttatgccgcc tcggcgagca
      301 catggaacgg gttggcatgg attgtaggcg ccgccctata ccttgtctgc ctccccgcgt
      361 tgcgtcgcgg tgcatggagc cgggccacct cgacctgaat ggaagccggc ggcacctcgc
      421 taacggattc accactccaa gaattggagc caatcaattc ttgcggagaa ctgtgaatgc
      481 gcaaaccaac ccttggcaga acatatccat cgcgtccgcc atctccagca gccgcacgcg
      541 gcgcatctcg ggcagcgttg ggtcctggcc acgggtgcgc atgatcgtgc tcctgtcgtt
      601 gaggacccgg ctaggctggc ggggttgcct tactggttag cagaatgaat caccgatacg
      661 cgagcgaacg tgaagcgact gctgctgcaa aacgtctgcg acctgagcaa caacatgaat
      721 ggtcttcggt ttccgtgttt cgtaaagtct ggaaacgcgg aagtcagcgc cctgcaccat
      781 tatgttccgg atctgcatcg caggatgctg ctggctaccc tgtggaacac ctacatctgt
      841 attaacgaag cgctggcatt gaccctgagt gatttttctc tggtcccgcc gcatccatac
      901 cgccagttgt ttaccctcac aacgttccag taaccgggca tgttcatcat cagtaacccg
      961 tatcgtgagc atcctctctc gtttcatcgg tatcattacc cccatgaaca gaaatccccc
     1021 ttacacggag gcatcagtga ccaaacagga aaaaaccgcc cttaacatgg cccgctttat
     1081 cagaagccag acattaacgc ttctggagaa actcaacgag ctggacgcgg atgaacaggc
     1141 agacatctgt gaatcgcttc acgaccacgc tgatgagctt taccgcagct gcctcgcgcg
     1201 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg
     1261 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg
     1321 gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata ctggcttaac
     1381 tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac
     1441 agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg
     1501 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     1561 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     1621 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     1681 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     1741 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     1801 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     1861 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     1921 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     1981 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     2041 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
     2101 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     2161 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     2221 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     2281 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     2341 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     2401 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     2461 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     2521 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     2581 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     2641 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     2701 aatagtttgc gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt
     2761 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     2821 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     2881 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     2941 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     3001 cggcgaccga gttgctcttg cccggcgtca acacgggata ataccgcgcc acatagcaga
     3061 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     3121 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     3181 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     3241 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     3301 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     3361 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgtcta gctacgaatt
     3421 cttcgacagc ttcgaaacta gaatcgattt cgaaactagc ttaagggtgg gaaagaatat
     3481 ataaggtggg ggtcttatgt agttttgtat ctgttttgca gcagccgccg ccgccatgag
     3541 caccaactcg tttgatggaa gcattgtgag ctcatatttg acaacgcgca tgcccccatg
     3601 ggccggggtg cgtcagaatg tgatgggctc cagcattgat ggtcgccccg tcctgcccgc
     3661 aaactctact accttgacct acgagaccgt gtctggaacg ccgttggaga ctgcagcctc
     3721 cgccgccgct tcagccgctg cagccaccgc ccgcgggatt gtgactgact ttgctttcct
     3781 gagcccgctt gcaagcagtg cagcttcccg ttcatccgcc cgcgatgaca agttgacggc
     3841 tcttttggca caattggatt ctttgacccg ggaacttaat gtcgtttctc agcagctgtt
     3901 ggatctgcgc cagcaggttt ctgccctgaa ggcttcctcc cctcccaatg cggtttaaaa
     3961 cataaataaa aaaccagact ctgtttggat ttggatcaag caagtgtctt gctgtcttta
     4021 tttaggggtt ttgcgcgcgc ggtaggcccg ggaccagcgg tctcggtcgt tgagggtcct
     4081 gtgtattttt tccaggacgt ggtaaaggtg actctggatg ttcagataca tgggcataag
     4141 cccgtctctg gggtggaggt agcaccactg cagagcttca tgctgcgggg tggtgttgta
     4201 gatgatccag tcgtagcagg agcgctgggc gtggtgccta aaaatgtctt tcagtagcaa
     4261 gctgattgcc aggggcaggc ccttggtgta agtgtttaca aagcggttaa gctgggatgg
     4321 gtgcatacgt ggggatatga gatgcatctt ggactgtatt tttaggttgg ctatgttccc
     4381 agccatatcc ctccggggat tcatgttgtg cagaaccacc agcacagtgt atccggtgca
     4441 cttgggaaat ttgtcatgta gcttagaagg aaatgcgtgg aagaacttgg agacgccctt
     4501 gtgacctcca agattttcca tgcattcgtc cataatgatg gcaatgggcc cacgggcggc
     4561 ggcctgggcg aagatatttc tgggatcact aacgtcatag ttgtgttcca ggatgagatc
     4621 gtcataggcc atttttacaa agcgcgggcg gagggtgcca gactgcggta taatggttcc
     4681 atccggccca ggggcgtagt taccctcaca gatttgcatt tcccacgctt tgagttcaga
     4741 tggggggatc atgtctacct gcggggcgat gaagaaaacg gtttccgggg taggggagat
     4801 cagctgggaa gaaagcaggt tcctgagcag ctgcgactta ccgcagccgg tgggcccgta
     4861 aatcacacct attaccggct gcaactggta gttaagagag ctgcagctgc cgtcatccct
     4921 gagcaggggg gccacttcgt taagcatgtc cctgactcgc atgttttccc tgaccaaatc
     4981 cgccagaagg cgctcgccgc ccagcgatag cagttcttgc aaggaagcaa agtttttcaa
     5041 cggtttgaga ccgtccgccg taggcatgct tttgagcgtt tgaccaagca gttccaggcg
     5101 gtcccacagc tcggtcacct gctctacggc atctcgatcc agcatatctc ctcgtttcgc
     5161 gggttggggc ggctttcgct gtacggcagt agtcggtgct cgtccagacg ggccagggtc
     5221 atgtctttcc acgggcgcag ggtcctcgtc agcgtagtct gggtcacggt gaaggggtgc
     5281 gctccgggct gcgcgctggc cagggtgcgc ttgaggctgg tcctgctggt gctgaagcgc
     5341 tgccggtctt cgccctgcgc gtcggccagg tagcatttga ccatggtgtc atagtccagc
     5401 ccctccgcgg cgtggccctt ggcgcgcagc ttgcccttgg aggaggcgcc gcacgagggg
     5461 cagtgcagac ttttgagggc gtagagcttg ggcgcgagaa ataccgattc cggggagtag
     5521 gcatccgcgc cgcaggcccc gcagacggtc tcgcattcca cgagccaggt gagctctggc
     5581 cgttcggggt caaaaaccag gtttccccca tgctttttga tgcgtttctt acctctggtt
     5641 tccatgagcc ggtgtccacg ctcggtgacg aaaaggctgt ccgtgtcccc gtatacagac
     5701 ttgagaggcc tgtcctcgag cggtgttccg cggtcctcct cgtatagaaa ctcggaccac
     5761 tctgagacaa aggctcgcgt ccaggccagc acgaaggagg ctaagtggga ggggtagcgg
     5821 tcgttgtcca ctagggggtc cactcgctcc agggtgtgaa gacacatgtc gccctcttcg
     5881 gcatcaagga aggtgattgg tttgtaggtg taggccacgt gaccgggtgt tcctgaaggg
     5941 gggctataaa agggggtggg ggcgcgttcg tcctcactct cttccgcatc gctgtctgcg
     6001 agggccagct gttggggtga gtactccctc tgaaaagcgg gcatgacttc tgcgctaaga
     6061 ttgtcagttt ccaaaaacga ggaggatttg atattcacct ggcccgcggt gatgcctttg
     6121 agggtggccg catccatctg gtcagaaaag acaatctttt tgttgtcaag cttggtggca
     6181 aacgacccgt agagggcgtt ggacagcaac ttggcgatgg agcgcagggt ttggtttttg
     6241 tcgcgatcgg cgcgctcctt ggccgcgatg tttagctgca cgtattcgcg cgcaacgcac
     6301 cgccattcgg gaaagacggt ggtgcgctcg tcgggcacca ggtgcacgcg ccaaccgcgg
     6361 ttgtgcaggg tgacaaggtc aacgctggtg gctacctctc cgcgtaggcg ctcgttggtc
     6421 cagcagaggc ggccgccctt gcgcgagcag aatggcggta gggggtctag ctgcgtctcg
     6481 tccggggggt ctgcgtccac ggtaaagacc ccgggcagca ggcgcgcgtc gaagtagtct
     6541 atcttgcatc cttgcaagtc tagcgcctgc tgccatgcgc gggcggcaag cgcgcgctcg
     6601 tatgggttga gtgggggacc ccatggcatg gggtgggtga gcgcggaggc gtacatgccg
     6661 caaatgtcgt aaacgtagag gggctctctg agtattccaa gatatgtagg gtagcatctt
     6721 ccaccgcgga tgctggcgcg cacgtaatcg tatagttcgt gcgagggagc gaggaggtcg
     6781 ggaccgaggt tgctacgggc gggctgctct gctcggaaga ctatctgcct gaagatggca
     6841 tgtgagttgg atgatatggt tggacgctgg aagacgttga agctggcgtc tgtgagacct
     6901 accgcgtcac gcacgaagga ggcgtaggag tcgcgcagct tgttgaccag ctcggcggtg
     6961 acctgcacgt ctagggcgca gtagtccagg gtttccttga tgatgtcata cttatcctgt
     7021 cccttttttt tccacagctc gcggttgagg acaaactctt cgcggtcttt ccagtactct
     7081 tggatcggaa acccgtcggc ctccgaacgg taagagccta gcatgtagaa ctggttgacg
     7141 gcctggtagg cgcagcatcc cttttctacg ggtagcgcgt atgcctgcgc ggccttccgg
     7201 agcgaggtgt gggtgagcgc aaaggtgtcc ctgaccatga ctttgaggta ctggtatttg
     7261 aagtcagtgt cgtcgcatcc gccctgctcc cagagcaaaa agtccgtgcg ctttttggaa
     7321 cgcggatttg gcagggcgaa ggtgacatcg ttgaagagta tctttcccgc gcgaggcata
     7381 aagttgcgtg tgatgcggaa gggtcccggc acctcggaac ggttgttaat tacctgggcg
     7441 gcgagcacga tctcgtcaaa gccgttgatg ttgtggccca caatgtaaag ttccaagaag
     7501 cgcgggatgc ccttgatgga aggcaatttt ttaagttcct cgtaggtgag ctcttcaggg
     7561 gagctgagcc cgtgctctga aagggcccag tctgcaagat gagggttgga agcgacgaat
     7621 gagctccaca ggtcacgggc cattagcatt tgcaggtggt cgcgaaaggt cctaaactgg
     7681 cgacctatgg ccattttttc tggggtgatg cagtagaagg taagcgggtc ttgttcccag
     7741 cggtcccatc caaggttcgc ggctaggtct cgcgcggcag tcactagagg ctcatctccg
     7801 ccgaacttca tgaccagcat gaagggcacg agctgcttcc caaaggcccc catccaagta
     7861 taggtctcta catcgtaggt gacaaagaga cgctcggtgc gaggatgcga gccgatcggg
     7921 aagaactgga tctcccgcca ccaattggag gagtggctat tgatgtggtg aaagtagaag
     7981 tccctgcgac gggccgaaca ctcgtgctgg cttttgtaaa aacgtgcgca gtactggcag
     8041 cggtgcacgg gctgtacatc ctgcacgagg ttgacctgac gaccgcgcac aaggaagcag
     8101 agtgggaatt tgagcccctc gcctggcggg tttggctggt ggtcttctac ttcggctgct
     8161 tgtccttgac cgtctggctg ctcgagggga gttacggtgg atcggaccac cacgccgcgc
     8221 gagcccaaag tccagatgtc cgcgcgcggc ggtcggagct tgatgacaac atcgcgcaga
     8281 tgggagctgt ccatggtctg gagctcccgc ggcgtcaggt caggcgggag ctcctgcagg
     8341 tttacctcgc atagacgggt cagggcgcgg gctagatcca ggtgatacct aatttccagg
     8401 ggctggttgg tggcggcgtc gatggcttgc aagaggccgc atccccgcgg cgcgactacg
     8461 gtaccgcgcg gcgggcggtg ggccgcgggg gtgtccttgg atgatgcatc taaaagcggt
     8521 gacgcgggcg agcccccgga ggtagggggg gctccggacc cgccgggaga gggggcaggg
     8581 gcacgtcggc gccgcgcgcg ggcaggagct ggtgctgcgc gcgtaggttg ctggcgaacg
     8641 cgacgacgcg gcggttgatc tcctgaatct ggcgcctctg cgtgaagacg acgggcccgg
     8701 tgagcttgaa cctgaaagag agttcgacag aatcaatttc ggtgtcgttg acggcggcct
     8761 ggcgcaaaat ctcctgcacg tctcctgagt tgtcttgata ggcgatctcg gccatgaact
     8821 gctcgatctc ttcctcctgg agatctccgc gtccggctcg ctccacggtg gcggcgaggt
     8881 cgttggaaat gcgggccatg agctgcgaga aggcgttgag gcctccctcg ttccagacgc
     8941 ggctgtagac cacgccccct tcggcatcgc gggcgcgcat gaccacctgc gcgagattga
     9001 gctccacgtg ccgggcgaag acggcgtagt ttcgcaggcg ctgaaagagg tagttgaggg
     9061 tggtggcggt gtgttctgcc acgaagaagt acataaccca gcgtcgcaac gtggattcgt
     9121 tgatatcccc caaggcctca aggcgctcca tggcctcgta gaagtccacg gcgaagttga
     9181 aaaactggga gttgcgcgcc gacacggtta actcctcctc cagaagacgg atgagctcgg
     9241 cgacagtgtc gcgcacctcg cgctcaaagg ctacaggggc ctcttcttct tcttcaatct
     9301 cctcttccat aagggcctcc ccttcttctt cttctggcgg cggtggggga ggggggacac
     9361 ggcggcgacg acggcgcacc gggaggcggt cgacaaagcg ctcgatcatc tccccgcggc
     9421 gacggcgcat ggtctcggtg acggcgcggc cgttctcgcg ggggcgcagt tggaagacgc
     9481 cgcccgtcat gtcccggtta tgggttggcg gggggctgcc atgcggcagg gatacggcgc
     9541 taacgatgca tctcaacaat tgttgtgtag gtactccgcc gccgagggac ctgagcgagt
     9601 ccgcatcgac cggatcggaa aacctctcga gaaaggcgtc taaccagtca cagtcgcaag
     9661 gtaggctgag caccgtggcg ggcggcagcg ggcggcggtc ggggttgttt ctggcggagg
     9721 tgctgctgat gatgtaatta aagtaggcgg tcttgagacg gcggatggtc gacagaagca
     9781 ccatgtcctt gggtccggcc tgctgaatgc gcaggcggtc ggccatgccc caggcttcgt
     9841 tttgacatcg gcgcaggtct ttgtagtagt cttgcatgag cctttctacc ggcacttctt
     9901 cttctccttc ctcttgtcct gcatctcttg catctatcgc tgcggcggcg gcggagtttg
     9961 gccgtaggtg gcgccctctt cctcccatgc gtgtgacccc gaagcccctc atcggctgaa
    10021 gcagggctag gtcggcgaca acgcgctcgg ctaatatggc ctgctgcacc tgcgtgaggg
    10081 tagactggaa gtcatccatg tccacaaagc ggtggtatgc gcccgtgttg atggtgtaag
    10141 tgcagttggc cataacggac cagttaacgg tctggtgacc cggctgcgag agctcggtgt
    10201 acctgagacg cgagtaagcc ctcgagtcaa atacgtagtc gttgcaagtc cgcaccaggt
    10261 actggtatcc caccaaaaag tgcggcggcg gctggcggta gaggggccag cgtagggtgg
    10321 ccggggctcc gggggcgaga tcttccaaca taaggcgatg atatccgtag atgtacctgg
    10381 acatccaggt gatgccggcg gcggtggtgg aggcgcgcgg aaagtcgcgg acgcggttcc
    10441 agatgttgcg cagcggcaaa aagtgctcca tggtcgggac gctctggccg gtcaggcgcg
    10501 cgcaatcgtt gacgctctag cgtgcaaaag gagagcctgt aagcgggcac tcttccgtgg
    10561 tctggtggat aaattcgcaa gggtatcatg gcggacgacc ggggttcgag ccccgtatcc
    10621 ggccgtccgc cgtgatccat gcggttaccg cccgcgtgtc gaacccaggt gtgcgacgtc
    10681 agacaacggg ggagtgctcc ttttggcttc cttccaggcg cggcggctgc tgcgctagct
    10741 tttttggcca ctggccgcgc gcagcgtaag cggttaggct ggaaagcgaa agcattaagt
    10801 ggctcgctcc ctgtagccgg agggttattt tccaagggtt gagtcgcggg acccccggtt
    10861 cgagtctcgg accggccgga ctgcggcgaa cgggggtttg cctccccgtc atgcaagacc
    10921 ccgcttgcaa attcctccgg aaacagggac gagccccttt tttgcttttc ccagatgcat
    10981 ccggtgctgc ggcagatgcg cccccctcct cagcagcggc aagagcaaga gcagcggcag
    11041 acatgcaggg caccctcccc tcctcctacc gcgtcaggag gggcgacatc cgcggttgac
    11101 gcggcagcag atggtgatta cgaacccccg cggcgccggg cccggcacta cctggacttg
    11161 gaggagggcg agggcctggc gcggctagga gcgccctctc ctgagcggca cccaagggtg
    11221 cagctgaagc gtgatacgcg tgaggcgtac gtgccgcggc agaacctgtt tcgcgaccgc
    11281 gagggagagg agcccgagga gatgcgggat cgaaagttcc acgcagggcg cgagctgcgg
    11341 catggcctga atcgcgagcg gttgctgcgc gaggaggact ttgagcccga cgcgcgaacc
    11401 gggattagtc ccgcgcgcgc acacgtggcg gccgccgacc tggtaaccgc atacgagcag
    11461 acggtgaacc aggagattaa ctttcaaaaa agctttaaca accacgtgcg tacgcttgtg
    11521 gcgcgcgagg aggtggctat aggactgatg catctgtggg actttgtaag cgcgctggag
    11581 caaaacccaa atagcaagcc gctcatggcg cagctgttcc ttatagtgca gcacagcagg
    11641 gacaacgagg cattcaggga tgcgctgcta aacatagtag agcccgaggg ccgctggctg
    11701 ctcgatttga taaacatcct gcagagcata gtggtgcagg agcgcagctt gagcctggct
    11761 gacaaggtgg ccgccatcaa ctattccatg cttagcctgg gcaagtttta cgcccgcaag
    11821 atataccata ccccttacgt tcccatagac aaggaggtaa agatcgaggg gttctacatg
    11881 cgcatggcgc tgaaggtgct taccttgagc gacgacctgg gcgtttatcg caacgagcgc
    11941 atccacaagg ccgtgagcgt gagccggcgg cgcgagctca gcgaccgcga gctgatgcac
    12001 agcctgcaaa gggccctggc tggcacgggc agcggcgata gagaggccga gtcctacttt
    12061 gacgcgggcg ctgacctgcg ctgggcccca agccgacgcg ccctggaggc agctggggcc
    12121 ggacctgggc tggcggtggc acccgcgcgc gctggcaacg tcggcggcgt ggaggaatat
    12181 gacgaggacg atgagtacga gccagaggac ggcgagtact aagcggtgat gtttctgatc
    12241 agatgatgca agacgcaacg gacccggcgg tgcgggcggc gctgcagagc cagccgtccg
    12301 gccttaactc cacggacgac tggcgccagg tcatggaccg catcatgtcg ctgactgcgc
    12361 gcaatcctga cgcgttccgg cagcagccgc aggccaaccg gctctccgca attctggaag
    12421 cggtggtccc ggcgcgcgca aaccccacgc acgagaaggt gctggcgatc gtaaacgcgc
    12481 tggccgaaaa cagggccatc cggcccgacg aggccggcct ggtctacgac gcgctgcttc
    12541 agcgcgtggc tcgttacaac agcggcaacg tgcagaccaa cctggaccgg ctggtggggg
    12601 atgtgcgcga ggccgtggcg cagcgtgagc gcgcgcagca gcagggcaac ctgggctcca
    12661 tggttgcact aaacgccttc ctgagtacac agcccgccaa cgtgccgcgg ggacaggagg
    12721 actacaccaa ctttgtgagc gcactgcggc taatggtgac tgagacaccg caaagtgagg
    12781 tgtaccagtc tgggccagac tattttttcc agaccagtag acaaggcctg cagaccgtaa
    12841 acctgagcca ggctttcaaa aacttgcagg ggctgtgggg ggtgcgggct cccacaggcg
    12901 accgcgcgac cgtgtctagc ttgctgacgc ccaactcgcg cctgttgctg ctgctaatag
    12961 cgcccttcac ggacagtggc agcgtgtccc gggacacata cctaggtcac ttgctgacac
    13021 tgtaccgcga ggccataggt caggcgcatg tggacgagca tactttccag gagattacaa
    13081 gtgtcagccg cgcgctgggg caggaggaca cgggcagcct ggaggcaacc ctaaactacc
    13141 tgctgaccaa ccggcggcag aagatcccct cgttgcacag tttaaacagc gaggaggagc
    13201 gcattttgcg ctacgtgcag cagagcgtga gccttaacct gatgcgcgac ggggtaacgc
    13261 ccagcgtggc gctggacatg accgcgcgca acatggaacc gggcatgtat gcctcaaacc
    13321 ggccgtttat caaccgccta atggactact tgcatcgcgc ggccgccgtg aaccccgagt
    13381 atttcaccaa tgccatcttg aacccgcact ggctaccgcc ccctggtttc tacaccgggg
    13441 gattcgaggt gcccgagggt aacgatggat tcctctggga cgacatagac gacagcgtgt
    13501 tttccccgca accgcagacc ctgctagagt tgcaacagcg cgagcaggca gaggcggcgc
    13561 tgcgaaagga aagcttccgc aggccaagca gcttgtccga tctaggcgct gcggccccgc
    13621 ggtcagatgc tagtagccca tttccaagct tgatagggtc tcttaccagc actcgcacca
    13681 cccgcccgcg cctgctgggc gaggaggagt acctaaacaa ctcgctgctg cagccgcagc
    13741 gcgaaaaaaa cctgcctccg gcatttccca acaacgggat agagagccta gtggacaaga
    13801 tgagtagatg gaagacgtac gcgcaggagc acagggacgt gccaggcccg cgcccgccca
    13861 cccgtcgtca aaggcacgac cgtcagcggg gtctggtgtg ggaggacgat gactcggcag
    13921 acgacagcag cgtcctggat ttgggaggga gtggcaaccc gtttgcgcac cttcgcccca
    13981 ggctggggag aatgttttaa aaaaaaaaaa gcatgatgca aaataaaaaa ctcaccaagg
    14041 ccatggcacc gagcgttggt tttcttgtat tccccttagt atgcggcgcg cggcgatgta
    14101 tgaggaaggt cctcctccct cctacgagag tgtggtgagc gcggcgccag tggcggcggc
    14161 gctgggttct cccttcgatg ctcccctgga cccgccgttt gtgcctccgc ggtacctgcg
    14221 gcctaccggg gggagaaaca gcatccgtta ctctgagttg gcacccctat tcgacaccac
    14281 ccgtgtgtac ctggtggaca acaagtcaac ggatgtggca tccctgaact accagaacga
    14341 ccacagcaac tttctgacca cggtcattca aaacaatgac tacagcccgg gggaggcaag
    14401 cacacagacc atcaatcttg acgaccggtc gcactggggc ggcgacctga aaaccatcct
    14461 gcataccaac atgccaaatg tgaacgagtt catgtttacc aataagttta aggcgcgggt
    14521 gatggtgtcg cgcttgccta ctaaggacaa tcaggtggag ctgaaatacg agtgggtgga
    14581 gttcacgctg cccgagggca actactccga gaccatgacc atagacctta tgaacaacgc
    14641 gatcgtggag cactacttga aagtgggcag acagaacggg gttctggaaa gcgacatcgg
    14701 ggtaaagttt gacacccgca acttcagact ggggtttgac cccgtcactg gtcttgtcat
    14761 gcctggggta tatacaaacg aagccttcca tccagacatc attttgctgc caggatgcgg
    14821 ggtggacttc acccacagcc gcctgagcaa cttgttgggc atccgcaagc ggcaaccctt
    14881 ccaggagggc tttaggatca cctacgatga tctggagggt ggtaacattc ccgcactgtt
    14941 ggatgtggac gcctaccagg cgagcttgaa agatgacacc gaacagggcg ggggtggcgc
    15001 aggcggcagc aacagcagtg gcagcggcgc ggaagagaac tccaacgcgg cagccgcggc
    15061 aatgcagccg gtggaggaca tgaacgatca tgccattcgc ggcgacacct ttgccacacg
    15121 ggctgaggag aagcgcgctg aggccgaagc agcggccgaa gctgccgccc ccgctgcgca
    15181 acccgaggtc gagaagcctc agaagaaacc ggtgatcaaa cccctgacag aggacagcaa
    15241 gaaacgcagt tacaacctaa taagcaatga cagcaccttc acccagtacc gcagctggta
    15301 ccttgcatac aactacggcg accctcagac cggaatccgc tcatggaccc tgctttgcac
    15361 tcctgacgta acctgcggct cggagcaggt ctactggtcg ttgccagaca tgatgcaaga
    15421 ccccgtgacc ttccgctcca cgcgccagat cagcaacttt ccggtggtgg gcgccgagct
    15481 gttgcccgtg cactccaaga gcttctacaa cgaccaggcc gtctactccc aactcatccg
    15541 ccagtttacc tctctgaccc acgtgttcaa tcgctttccc gagaaccaga ttttggcgcg
    15601 cccgccagcc cccaccatca ccaccgtcag tgaaaacgtt cctgctctca cagatcacgg
    15661 gacgctaccg ctgcgcaaca gcatcggagg agtccagcga gtgaccatta ctgacgccag
    15721 acgccgcacc tgcccctacg tttacaaggc cctgggcata gtctcgccgc gcgtcctatc
    15781 gagccgcact ttttgagcaa gcatgtccat ccttatatcg cccagcaata acacaggctg
    15841 gggcctgcgc ttcccaagca agatgtttgg cggggccaag aagcgctccg accaacaccc
    15901 agtgcgcgtg cgcgggcact accgcgcgcc ctggggcgcg cacaaacgcg gccgcactgg
    15961 gcgcaccacc gtcgatgacg ccatcgacgc ggtggtggag gaggcgcgca actacacgcc
    16021 cacgccgcca ccagtgtcca cagtggacgc ggccattcag accgtggtgc gcggagcccg
    16081 gcgctatgct aaaatgaaga gacggcggag gcgcgtagca cgtcgccacc gccgccgacc
    16141 cggcactgcc gcccaacgcg cggcggcggc cctgcttaac cgcgcacgtc gcaccggccg
    16201 acgggcggcc atgcgggccg ctcgaaggct ggccgcgggt attgtcactg tgccccccag
    16261 gtccaggcga cgagcggccg ccgcagcagc cgcggccatt agtgctatga ctcagggtcg
    16321 caggggcaac gtgtattggg tgcgcgactc ggttagcggc ctgcgcgtgc ccgtgcgcac
    16381 ccgccccccg cgcaactaga ttgcaagaaa aaactactta gactcgtact gttgtatgta
    16441 tccagcggcg gcggcgcgca acgaagctat gtccaagcgc aaaatcaaag aagagatgct
    16501 ccaggtcatc gcgccggaga tctatggccc cccgaagaag gaagagcagg attacaagcc
    16561 ccgaaagcta aagcgggtca aaaagaaaaa gaaagatgat gatgatgaac ttgacgacga
    16621 ggtggaactg ctgcacgcta ccgcgcccag gcgacgggta cagtggaaag gtcgacgcgt
    16681 aaaacgtgtt ttgcgacccg gcaccaccgt agtctttacg cccggtgagc gctccacccg
    16741 cacctacaag cgcgtgtatg atgaggtgta cggcgacgag gacctgcttg agcaggccaa
    16801 cgagcgcctc ggggagtttg cctacggaaa gcggcataag gacatgctgg cgttgccgct
    16861 ggacgagggc aacccaacac ctagcctaaa gcccgtaaca ctgcagcagg tgctgcccgc
    16921 gcttgcaccg tccgaagaaa agcgcggcct aaagcgcgag tctggtgact tggcacccac
    16981 cgtgcagctg atggtaccca agcgccagcg actggaagat gtcttggaaa aaatgaccgt
    17041 ggaacctggg ctggagcccg aggtccgcgt gcggccaatc aagcaggtgg cgccgggact
    17101 gggcgtgcag accgtggacg ttcagatacc cactaccagt agcaccagta ttgccaccgc
    17161 cacagagggc atggagacac aaacgtcccc ggttgcctca gcggtggcgg atgccgcggt
    17221 gcaggcggtc gctgcggccg cgtccaagac ctctacggag gtgcaaacgg acccgtggat
    17281 gtttcgcgtt tcagcccccc ggcgcccgcg ccgttcgagg aagtacggcg ccgccagcgc
    17341 gctactgccc gaatatgccc tacatccttc cattgcgcct acccccggct atcgtggcta
    17401 cacctaccgc cccagaagac gagcaactac ccgacgccga accaccactg gaacccgccg
    17461 ccgccgtcgc cgtcgccagc ccgtgctggc cccgatttcc gtgcgcaggg tggctcgcga
    17521 aggaggcagg accctggtgc tgccaacagc gcgctaccac cccagcatcg tttaaaagcc
    17581 ggtctttgtg gttcttgcag atatggccct cacctgccgc ctccgtttcc cggtgccggg
    17641 attccgagga agaatgcacc gtaggagggg catggccggc cacggcctga cgggcggcat
    17701 gcgtcgtgcg caccaccggc ggcggcgcgc gtcgcaccgt cgcatgcgcg gcggtatcct
    17761 gcccctcctt attccactga tcgccgcggc gattggcgcc gtgcccggaa ttgcatccgt
    17821 ggccttgcag gcgcagagac actgattaaa aacaagttgc atgtggaaaa atcaaaataa
    17881 aaagtctgga ctctcacgct cgcttggtcc tgtaactatt ttgtagaatg gaagacatca
    17941 actttgcgtc tctggccccg cgacacggct cgcgcccgtt catgggaaac tggcaagata
    18001 tcggcaccag caatatgagc ggtggcgcct tcagctgggg ctcgctgtgg agcggcatta
    18061 aaaatttcgg ttccaccgtt aagaactatg gcagcaaggc ctggaacagc agcacaggcc
    18121 agatgctgag ggataagttg aaagagcaaa atttccaaca aaaggtggta gatggcctgg
    18181 cctctggcat tagcggggtg gtggacctgg ccaaccaggc agtgcaaaat aagattaaca
    18241 gtaagcttga tccccgccct cccgtagagg agcctccacc ggccgtggag acagtgtctc
    18301 cagaggggcg tggcgaaaag cgtccgcgcc ccgacaggga agaaactctg gtgacgcaaa
    18361 tagacgagcc tccctcgtac gaggaggcac taaagcaagg cctgcccacc acccgtccca
    18421 tcgcgcccat ggctaccgga gtgctgggcc agcacacacc cgtaacgctg gacctgcctc
    18481 cccccgccga cacccagcag aaacctgtgc tgccaggccc gaccgccgtt gttgtaaccc
    18541 gtcctagccg cgcgtccctg cgccgcgccg ccagcggtcc gcgatcgttg cggcccgtag
    18601 ccagtggcaa ctggcaaagc acactgaaca gcatcgtggg tctgggggtg caatccctga
    18661 agcgccgacg atgcttctga tagctaacgt gtcgtatgtg tgtcatgtat gcgtccatgt
    18721 cgccgccaga ggagctgctg agccgccgcg cgcccgcttt ccaagatggc taccccttcg
    18781 atgatgccgc agtggtctta catgcacatc tcgggccagg acgcctcgga gtacctgagc
    18841 cccgggctgg tgcagtttgc ccgcgccacc gagacgtact tcagcctgaa taacaagttt
    18901 agaaacccca cggtggcgcc tacgcacgac gtgaccacag accggtccca gcgtttgacg
    18961 ctgcggttca tccctgtgga ccgtgaggat actgcgtact cgtacaaggc gcggttcacc
    19021 ctagctgtgg gtgataaccg tgtgctggac atggcttcca cgtactttga catccgcggc
    19081 gtgctggaca ggggccctac ttttaagccc tactctggca ctgcctacaa cgccctggct
    19141 cccaagggtg ccccaaatcc ttgcgaatgg gatgaagctg ctactgctct tgaaataaac
    19201 ctagaagaag aggacgatga caacgaagac gaagtagacg agcaagctga gcagcaaaaa
    19261 actcacgtat ttgggcaggc gccttattct ggtataaata ttacaaagga gggtattcaa
    19321 ataggtgtcg aaggtcaaac acctaaatat gccgataaaa catttcaacc tgaacctcaa
    19381 ataggagaat ctcagtggta cgaaacagaa attaatcatg cagctgggag agtcctaaaa
    19441 aagactaccc caatgaaacc atgttacggt tcatatgcaa aacccacaaa tgaaaatgga
    19501 gggcaaggca ttcttgtaaa gcaacaaaat ggaaagctag aaagtcaagt ggaaatgcaa
    19561 tttttctcaa ctactgaggc agccgcaggc aatggtgata acttgactcc taaagtggta
    19621 ttgtacagtg aagatgtaga tatagaaacc ccagacactc atatttctta catgcccact
    19681 attaaggaag gtaactcacg agaactaatg ggccaacaat ctatgcccaa caggcctaat
    19741 tacattgctt ttagggacaa ttttattggt ctaatgtatt acaacagcac gggtaatatg
    19801 ggtgttctgg cgggccaagc atcgcagttg aatgctgttg tagatttgca agacagaaac
    19861 acagagcttt cataccagct tttgcttgat tccattggtg atagaaccag gtacttttct
    19921 atgtggaatc aggctgttga cagctatgat ccagatgtta gaattattga aaatcatgga
    19981 actgaagatg aacttccaaa ttactgcttt ccactgggag gtgtgattaa tacagagact
    20041 cttaccaagg taaaacctaa aacaggtcag gaaaatggat gggaaaaaga tgctacagaa
    20101 ttttcagata aaaatgaaat aagagttgga aataattttg ccatggaaat caatctaaat
    20161 gccaacctgt ggagaaattt cctgtactcc aacatagcgc tgtatttgcc cgacaagcta
    20221 aagtacagtc cttccaacgt aaaaatttct gataacccaa acacctacga ctacatgaac
    20281 aagcgagtgg tggctcccgg gctagtggac tgctacatta accttggagc acgctggtcc
    20341 cttgactata tggacaacgt caacccattt aaccaccacc gcaatgctgg cctgcgctac
    20401 cgctcaatgt tgctgggcaa tggtcgctat gtgcccttcc acatccaggt gcctcagaag
    20461 ttctttgcca ttaaaaacct ccttctcctg ccgggctcat acacctacga gtggaacttc
    20521 aggaaggatg ttaacatggt tctgcagagc tccctaggaa atgacctaag ggttgacgga
    20581 gccagcatta agtttgatag catttgcctt tacgccacct tcttccccat ggcccacaac
    20641 accgcctcca cgcttgaggc catgcttaga aacgacacca acgaccagtc ctttaacgac
    20701 tatctctccg ccgccaacat gctctaccct atacccgcca acgctaccaa cgtgcccata
    20761 tccatcccct cccgcaactg ggcggctttc cgcggctggg ccttcacgcg ccttaagact
    20821 aaggaaaccc catcactggg ctcgggctac gacccttatt acacctactc tggctctata
    20881 ccctacctag atggaacctt ttacctcaac cacaccttta agaaggtggc cattaccttt
    20941 gactcttctg tcagctggcc tggcaatgac cgcctgctta cccccaacga gtttgaaatt
    21001 aagcgctcag ttgacgggga gggttacaac gttgcccagt gtaacatgac caaagactgg
    21061 ttcctggtac aaatgctagc taactataac attggctacc agggcttcta tatcccagag
    21121 agctacaagg accgcatgta ctccttcttt agaaacttcc agcccatgag ccgtcaggtg
    21181 gtggatgata ctaaatacaa ggactaccaa caggtgggca tcctacacca acacaacaac
    21241 tctggatttg ttggctacct tgcccccacc atgcgcgaag gacaggccta ccctgctaac
    21301 ttcccctatc cgcttatagg caagaccgca gttgacagca ttacccagaa aaagtttctt
    21361 tgcgatcgca ccctttggcg catcccattc tccagtaact ttatgtccat gggcgcactc
    21421 acagacctgg gccaaaacct tctctacgcc aactccgccc acgcgctaga catgactttt
    21481 gaggtggatc ccatggacga gcccaccctt ctttatgttt tgtttgaagt ctttgacgtg
    21541 gtccgtgtgc accagccgca ccgcggcgtc atcgaaaccg tgtacctgcg cacgcccttc
    21601 tcggccggca acgccacaac ataaagaagc aagcaacatc aacaacagct gccgccatgg
    21661 gctccagtga gcaggaactg aaagccattg tcaaagatct tggttgtggg ccatattttt
    21721 tgggcaccta tgacaagcgc tttccaggct ttgtttctcc acacaagctc gcctgcgcca
    21781 tagtcaatac ggccggtcgc gagactgggg gcgtacactg gatggccttt gcctggaacc
    21841 cgcactcaaa aacatgctac ctctttgagc cctttggctt ttctgaccag cgactcaagc
    21901 aggtttacca gtttgagtac gagtcactcc tgcgccgtag cgccattgct tcttcccccg
    21961 accgctgtat aacgctggaa aagtccaccc aaagcgtaca ggggcccaac tcggccgcct
    22021 gtggactatt ctgctgcatg tttctccacg cctttgccaa ctggccccaa actcccatgg
    22081 atcacaaccc caccatgaac cttattaccg gggtacccaa ctccatgctc aacagtcccc
    22141 aggtacagcc caccctgcgt cgcaaccagg aacagctcta cagcttcctg gagcgccact
    22201 cgccctactt ccgcagccac agtgcgcaga ttaggagcgc cacttctttt tgtcacttga
    22261 aaaacatgta aaaataatgt actagagaca ctttcaataa aggcaaatgc ttttatttgt
    22321 acactctcgg gtgattattt acccccaccc ttgccgtctg cgccgtttaa aaatcaaagg
    22381 ggttctgccg cgcatcgcta tgcgccactg gcagggacac gttgcgatac tggtgtttag
    22441 tgctccactt aaactcaggc acaaccatcc gcggcagctc ggtgaagttt tcactccaca
    22501 ggctgcgcac catcaccaac gcgtttagca ggtcgggcgc cgatatcttg aagtcgcagt
    22561 tggggcctcc gccctgcgcg cgcgagttgc gatacacagg gttgcagcac tggaacacta
    22621 tcagcgccgg gtggtgcacg ctggccagca cgctcttgtc ggagatcaga tccgcgtcca
    22681 ggtcctccgc gttgctcagg gcgaacggag tcaactttgg tagctgcctt cccaaaaagg
    22741 gcgcgtgccc aggctttgag ttgcactcgc accgtagtgg catcaaaagg tgaccgtgcc
    22801 cggtctgggc gttaggatac agcgcctgca taaaagcctt gatctgctta aaagccacct
    22861 gagcctttgc gccttcagag aagaacatgc cgcaagactt gccggaaaac tgattggccg
    22921 gacaggccgc gtcgtgcacg cagcaccttg cgtcggtgtt ggagatctgc accacatttc
    22981 ggccccaccg gttcttcacg atcttggcct tgctagactg ctccttcagc gcgcgctgcc
    23041 cgttttcgct cgtcacatcc atttcaatca cgtgctcctt atttatcata atgcttccgt
    23101 gtagacactt aagctcgcct tcgatctcag cgcagcggtg cagccacaac gcgcagcccg
    23161 tgggctcgtg atgcttgtag gtcacctctg caaacgactg caggtacgcc tgcaggaatc
    23221 gccccatcat cgtcacaaag gtcttgttgc tggtgaaggt cagctgcaac ccgcggtgct
    23281 cctcgttcag ccaggtcttg catacggccg ccagagcttc cacttggtca ggcagtagtt
    23341 tgaagttcgc ctttagatcg ttatccacgt ggtacttgtc catcagcgcg cgcgcagcct
    23401 ccatgccctt ctcccacgca gacacgatcg gcacactcag cgggttcatc accgtaattt
    23461 cactttccgc ttcgctgggc tcttcctctt cctcttgcgt ccgcatacca cgcgccactg
    23521 ggtcgtcttc attcagccgc cgcactgtgc gcttacctcc tttgccatgc ttgattagca
    23581 ccggtgggtt gctgaaaccc accatttgta gcgccacatc ttctctttct tcctcgctgt
    23641 ccacgattac ctctggtgat ggcgggcgct cgggcttggg agaagggcgc ttctttttct
    23701 tcttgggcgc aatggccaaa tccgccgccg aggtcgatgg ccgcgggctg ggtgtgcgcg
    23761 gcaccagcgc gtcttgtgat gagtcttcct cgtcctcgga ctcgatacgc cgcctcatcc
    23821 gcttttttgg gggcgcccgg ggaggcggcg gcgacgggga cggggacgac acgtcctcca
    23881 tggttggggg acgtcgcgcc gcaccgcgtc cgcgctcggg ggtggtttcg cgctgctcct
    23941 cttcccgact ggccatttcc ttctcctata ggcagaaaaa gatcatggag tcagtcgaga
    24001 agaaggacag cctaaccgcc ccctctgagt tcgccaccac cgcctccacc gatgccgcca
    24061 acgcgcctac caccttcccc gtcgaggcac ccccgcttga ggaggaggaa gtgattatcg
    24121 agcaggaccc aggttttgta agcgaagacg acgaggaccg ctcagtacca acagaggata
    24181 aaaagcaaga ccaggacaac gcagaggcaa acgaggaaca agtcgggcgg ggggacgaaa
    24241 ggcatggcga ctacctagat gtgggagacg acgtgctgtt gaagcatctg cagcgccagt
    24301 gcgccattat ctgcgacgcg ttgcaagagc gcagcgatgt gcccctcgcc atagcggatg
    24361 tcagccttgc ctacgaacgc cacctattct caccgcgcgt accccccaaa cgccaagaaa
    24421 acggcacatg cgagcccaac ccgcgcctca acttctaccc cgtatttgcc gtgccagagg
    24481 tgcttgccac ctatcacatc tttttccaaa actgcaagat acccctatcc tgccgtgcca
    24541 accgcagccg agcggacaag cagctggcct tgcggcaggg cgctgtcata cctgatatcg
    24601 cctcgctcaa cgaagtgcca aaaatctttg agggtcttgg acgcgacgag aagcgcgcgg
    24661 caaacgctct gcaacaggaa aacagcgaaa atgaaagtca ctctggagtg ttggtggaac
    24721 tcgagggtga caacgcgcgc ctagccgtac taaaacgcag catcgaggtc acccactttg
    24781 cctacccggc acttaaccta ccccccaagg tcatgagcac agtcatgagt gagctgatcg
    24841 tgcgccgtgc gcagcccctg gagagggatg caaatttgca agaacaaaca gaggagggcc
    24901 tacccgcagt tggcgacgag cagctagcgc gctggcttca aacgcgcgag cctgccgact
    24961 tggaggagcg acgcaaacta atgatggccg cagtgctcgt taccgtggag cttgagtgca
    25021 tgcagcggtt ctttgctgac ccggagatgc agcgcaagct agaggaaaca ttgcactaca
    25081 cctttcgaca gggctacgta cgccaggcct gcaagatctc caacgtggag ctctgcaacc
    25141 tggtctccta ccttggaatt ttgcacgaaa accgccttgg gcaaaacgtg cttcattcca
    25201 cgctcaaggg cgaggcgcgc cgcgactacg tccgcgactg cgtttactta tttctatgct
    25261 acacctggca gacggccatg ggcgtttggc agcagtgctt ggaggagtgc aacctcaagg
    25321 agctgcagaa actgctaaag caaaacttga aggacctatg gacggccttc aacgagcgct
    25381 ccgtggccgc gcacctggcg gacatcattt tccccgaacg cctgcttaaa accctgcaac
    25441 agggtctgcc agacttcacc agtcaaagca tgttgcagaa ctttaggaac tttatcctag
    25501 agcgctcagg aatcttgccc gccacctgct gtgcacttcc tagcgacttt gtgcccatta
    25561 agtaccgcga atgccctccg ccgctttggg gccactgcta ccttctgcag ctagccaact
    25621 accttgccta ccactctgac ataatggaag acgtgagcgg tgacggtcta ctggagtgtc
    25681 actgtcgctg caacctatgc accccgcacc gctccctggt ttgcaattcg cagctgctta
    25741 acgaaagtca aattatcggt acctttgagc tgcagggtcc ctcgcctgac gaaaagtccg
    25801 cggctccggg gttgaaactc actccggggc tgtggacgtc ggcttacctt cgcaaatttg
    25861 tacctgagga ctaccacgcc cacgagatta ggttctacga agaccaatcc cgcccgccta
    25921 atgcggagct taccgcctgc gtcattaccc agggccacat tcttggccaa ttgcaagcca
    25981 tcaacaaagc ccgccaagag tttctgctac gaaagggacg gggggtttac ttggaccccc
    26041 agtccggcga ggagctcaac ccaatccccc cgccgccgca gccctatcag cagcagccgc
    26101 gggcccttgc ttcccaggat ggcacccaaa aagaagctgc agctgccgcc gccacccacg
    26161 gacgaggagg aatactggga cagtcaggca gaggaggttt tggacgagga ggaggaggac
    26221 atgatggaag actgggagag cctagacgag gaagcttccg aggtcgaaga ggtgtcagac
    26281 gaaacaccgt caccctcggt cgcattcccc tcgccggcgc cccagaaatc ggcaaccggt
    26341 tccagcatgg ctacaacctc cgctcctcag gcgccgccgg cactgcccgt tcgccgaccc
    26401 aaccgtagat gggacaccac tggaaccagg gccggtaagt ccaagcagcc gccgccgtta
    26461 gcccaagagc aacaacagcg ccaaggctac cgctcatggc gcgggcacaa gaacgccata
    26521 gttgcttgct tgcaagactg tgggggcaac atctccttcg cccgccgctt tcttctctac
    26581 catcacggcg tggccttccc ccgtaacatc ctgcattact accgtcatct ctacagccca
    26641 tactgcaccg gcggcagcgg cagcaacagc agcggccaca cagaagcaaa ggcgaccgga
    26701 tagcaagact ctgacaaagc ccaagaaatc cacagcggcg gcagcagcag gaggaggagc
    26761 gctgcgtctg gcgcccaacg aacccgtatc gacccgcgag cttagaaaca ggatttttcc
    26821 cactctgtat gctatatttc aacagagcag gggccaagaa caagagctga aaataaaaaa
    26881 caggtctctg cgatccctca cccgcagctg cctgtatcac aaaagcgaag atcagcttcg
    26941 gcgcacgctg gaagacgcgg aggctctctt cagtaaatac tgcgcgctga ctcttaagga
    27001 ctagtttcgc gccctttctc aaatttaagc gcgaaaacta cgtcatctcc agcggccaca
    27061 cccggcgcca gcacctgttg tcagcgccat tatgagcaag gaaattccca cgccctacat
    27121 gtggagttac cagccacaaa tgggacttgc ggctggagct gcccaagact actcaacccg
    27181 aataaactac atgagcgcgg gaccccacat gatatcccgg gtcaacggaa tacgcgccca
    27241 ccgaaaccga attctcctgg aacaggcggc tattaccacc acacctcgta ataaccttaa
    27301 tccccgtagt tggcccgctg ccctggtgta ccaggaaagt cccgctccca ccactgtggt
    27361 acttcccaga gacgcccagg ccgaagttca gatgactaac tcaggggcgc agcttgcggg
    27421 cggctttcgt cacagggtgc ggtcgcccgg gcagggtata actcacctga caatcagagg
    27481 gcgaggtatt cagctcaacg acgagtcggt gagctcctcg cttggtctcc gtccggacgg
    27541 gacatttcag atcggcggcg ccggccgctc ttcattcacg cctcgtcagg caatcctaac
    27601 tctgcagacc tcgtcctctg agccgcgctc tggaggcatt ggaactctgc aatttattga
    27661 ggagtttgtg ccatcggtct actttaaccc cttctcggga cctcccggcc actatccgga
    27721 tcaatttatt cctaactttg acgcggtaaa ggactcggcg gacggctacg actgaatgtt
    27781 aagtggagag gcagagcaac tgcgcctgaa acacctggtc cactgtcgcc gccacaagtg
    27841 ctttgcccgc gactccggtg agttttgcta ctttgaattg cccgaggatc atatcgaggg
    27901 cccggcgcac ggcgtccggc ttaccgccca gggagagctt gcccgtagcc tgattcggga
    27961 gtttacccag cgccccctgc tagttgagcg ggacagggga ccctgtgttc tcactgtgat
    28021 ttgcaactgt cctaaccctg gattacatca agatcctcta gttaatgtca ggtcgcctaa
    28081 gtcgattaac tagagtaccc ggggatctta ttccctttaa ctaataaaaa aaaataataa
    28141 agcatcactt acttaaaatc agttagcaaa tttctgtcca gtttattcag cagcacctcc
    28201 ttgccctcct cccagctctg gtattgcagc ttcctcctgg ctgcaaactt tctccacaat
    28261 ctaaatggaa tgtcagtttc ctcctgttcc tgtccatccg cacccactat cttcatgttg
    28321 ttgcagatga agcgcgcaag accgtctgaa gataccttca accccgtgta tccatatgac
    28381 acggaaaccg gtcctccaac tgtgcctttt cttactcctc cctttgtatc ccccaatggg
    28441 tttcaagaga gtccccctgg ggtactctct ttgcgcctat ccgaacctct agttacctcc
    28501 aatggcatgc ttgcgctcaa aatgggcaac ggcctctctc tggacgaggc cggcaacctt
    28561 acctcccaaa atgtaaccac tgtgagccca cctctcaaaa aaaccaagtc aaacataaac
    28621 ctggaaatat ctgcacccct cacagttacc tcagaagccc taactgtggc tgccgccgca
    28681 cctctaatgg tcgcgggcaa cacactcacc atgcaatcac aggccccgct aaccgtgcac
    28741 gactccaaac ttagcattgc cacccaagga cccctcacag tgtcagaagg aaagctagcc
    28801 ctgcaaacat caggccccct caccaccacc gatagcagta cccttactat cactgcctca
    28861 ccccctctaa ctactgccac tggtagcttg ggcattgact tgaaagagcc catttataca
    28921 caaaatggaa aactaggact aaagtacggg gctcctttgc atgtaacaga cgacctaaac
    28981 actttgaccg tagcaactgg tccaggtgtg actattaata atacttcctt gcaaactaaa
    29041 gttactggag ccttgggttt tgattcacaa ggcaatatgc aacttaatgt agcaggagga
    29101 ctaaggattg attctcaaaa cagacgcctt atacttgatg ttagttatcc gtttgatgct
    29161 caaaaccaac taaatctaag actaggacag ggccctcttt ttataaactc agcccacaac
    29221 ttggatatta actacaacaa aggcctttac ttgtttacag cttcaaacaa ttccaaaaag
    29281 cttgaggtta acctaagcac tgccaagggg ttgatgtttg acgctacagc catagccatt
    29341 aatgcaggag atgggcttga atttggttca cctaatgcac caaacacaaa tcccctcaaa
    29401 acaaaaattg gccatggcct agaatttgat tcaaacaagg ctatggttcc taaactagga
    29461 actggcctta gttttgacag cacaggtgcc attacagtag gaaacaaaaa taatgataag
    29521 ctaactttgt ggaccacacc agctccatct cctaactgta gactaaatgc agagaaagat
    29581 gctaaactca ctttggtctt aacaaaatgt ggcagtcaaa tacttgctac agtttcagtt
    29641 ttggctgtta aaggcagttt ggctccaata tctggaacag ttcaaagtgc tcatcttatt
    29701 ataagatttg acgaaaatgg agtgctacta aacaattcct tcctggaccc agaatattgg
    29761 aactttagaa atggagatct tactgaaggc acagcctata caaacgctgt tggatttatg
    29821 cctaacctat cagcttatcc aaaatctcac ggtaaaactg ccaaaagtaa cattgtcagt
    29881 caagtttact taaacggaga caaaactaaa cctgtaacac taaccattac actaaacggt
    29941 acacaggaaa caggagacac aactccaagt gcatactcta tgtcattttc atgggactgg
    30001 tctggccaca actacattaa tgaaatattt gccacatcct cttacacttt ttcatacatt
    30061 gcccaagaat aaagaatcgt ttgtgttatg tttcaacgtg tttatttttc aattgcagaa
    30121 aatttcaagt catttttcat tcagtagtat agccccacca ccacatagct tatacagatc
    30181 accgtacctt aatcaaactc acagaaccct agtattcaac ctgccacctc cctcccaaca
    30241 cacagagtac acagtccttt ctccccggct ggccttaaaa agcatcatat catgggtaac
    30301 agacatattc ttaggtgtta tattccacac ggtttcctgt cgagccaaac gctcatcagt
    30361 gatattaata aactccccgg gcagctcact taagttcatg tcgctgtcca gctgctgagc
    30421 cacaggctgc tgtccaactt gcggttgctt aacgggcggc gaaggagaag tccacgccta
    30481 catgggggta gagtcataat cgtgcatcag gatagggcgg tggtgctgca gcagcgcgcg
    30541 aataaactgc tgccgccgcc gctccgtcct gcaggaatac aacatggcag tggtctcctc
    30601 agcgatgatt cgcaccgccc gcagcataag gcgccttgtc ctccgggcac agcagcgcac
    30661 cctgatctca cttaaatcag cacagtaact gcagcacagc accacaatat tgttcaaaat
    30721 cccacagtgc aaggcgctgt atccaaagct catggcgggg accacagaac ccacgtggcc
    30781 atcataccac aagcgcaggt agattaagtg gcgacccctc ataaacacgc tggacataaa
    30841 cattacctct tttggcatgt tgtaattcac cacctcccgg taccatataa acctctgatt
    30901 aaacatggcg ccatccacca ccatcctaaa ccagctggcc aaaacctgcc cgccggctat
    30961 acactgcagg gaaccgggac tggaacaatg acagtggaga gcccaggact cgtaaccatg
    31021 gatcatcatg ctcgtcatga tatcaatgtt ggcacaacac aggcacacgt gcatacactt
    31081 cctcaggatt acaagctcct cccgcgttag aaccatatcc cagggaacaa cccattcctg
    31141 aatcagcgta aatcccacac tgcagggaag acctcgcacg taactcacgt tgtgcattgt
    31201 caaagtgtta cattcgggca gcagcggatg atcctccagt atggtagcgc gggtttctgt
    31261 ctcaaaagga ggtagacgat ccctactgta cggagtgcgc cgagacaacc gagatcgtgt
    31321 tggtcgtagt gtcatgccaa atggaacgcc ggacgtagtc atatttcctg aagcaaaacc
    31381 aggtgcgggc gtgacaaaca gatctgcgtc tccggtctcg ccgcttagat cgctctgtgt
    31441 agtagttgta gtatatccac tctctcaaag catccaggcg ccccctggct tcgggttcta
    31501 tgtaaactcc ttcatgcgcc gctgccctga taacatccac caccgcagaa taagccacac
    31561 ccagccaacc tacacattcg ttctgcgagt cacacacggg aggagcggga agagctggaa
    31621 gaaccatgtt ttttttttta ttccaaaaga ttatccaaaa cctcaaaatg aagatctatt
    31681 aagtgaacgc gctcccctcc ggtggcgtgg tcaaactcta cagccaaaga acagataatg
    31741 gcatttgtaa gatgttgcac aatggcttcc aaaaggcaaa cggccctcac gtccaagtgg
    31801 acgtaaaggc taaacccttc agggtgaatc tcctctataa acattccagc accttcaacc
    31861 atgcccaaat aattctcatc tcgccacctt ctcaatatat ctctaagcaa atcccgaata
    31921 ttaagtccgg ccattgtaaa aatctgctcc agagcgccct ccaccttcag cctcaagcag
    31981 cgaatcatga ttgcaaaaat tcaggttcct cacagacctg tataagattc aaaagcggaa
    32041 cattaacaaa aataccgcga tcccgtaggt cccttcgcag ggccagctga acataatcgt
    32101 gcaggtctgc acggaccagc gcggccactt ccccgccagg aaccatgaca aaagaaccca
    32161 cactgattat gacacgcata ctcggagcta tgctaaccag cgtagccccg atgtaagctt
    32221 gttgcatggg cggcgatata aaatgcaagg tgctgctcaa aaaatcaggc aaagcctcgc
    32281 gcaaaaaaga aagcacatcg tagtcatgct catgcagata aaggcaggta agctccggaa
    32341 ccaccacaga aaaagacacc atttttctct caaacatgtc tgcgggtttc tgcataaaca
    32401 caaaataaaa taacaaaaaa acatttaaac attagaagcc tgtcttacaa caggaaaaac
    32461 aacccttata agcataagac ggactacggc catgccggcg tgaccgtaaa aaaactggtc
    32521 accgtgatta aaaagcacca ccgacagctc ctcggtcatg tccggagtca taatgtaaga
    32581 ctcggtaaac acatcaggtt gattcacatc ggtcagtgct aaaaagcgac cgaaatagcc
    32641 cgggggaata catacccgca ggcgtagaga caacattaca gcccccatag gaggtataac
    32701 aaaattaata ggagagaaaa acacataaac acctgaaaaa ccctcctgcc taggcaaaat
    32761 agcaccctcc cgctccagaa caacatacag cgcttccaca gcggcagcca taacagtcag
    32821 ccttaccagt aaaaaagaaa acctattaaa aaaacaccac tcgacacggc accagctcaa
    32881 tcagtcacag tgtaaaaaag ggccaagtgc agagcgagta tatataggac taaaaaatga
    32941 cgtaacggtt aaagtccaca aaaaacaccc agaaaaccgc acgcgaacct acgcccagaa
    33001 acgaaagcca aaaaacccac aacttcctca aatcgtcact tccgttttcc cacgttacgt
    33061 cacttcccat tttaagaaaa ctacaattcc caacacatac aagttactcc gccctaaaac
    33121 ctacgtcacc cgccccgttc ccacgccccg cgccacgtca caaactccac cccctcatta
    33181 tcatattggc ttcaatccaa aataaggtat attattgatg atgttaatta acatgcatgg
    33241 atcctacgtc tcgaccgatg cccttgagag ccttcaaccc agtcagctcc ttccggtggg
    33301 cgcggggcat gactatcgtc gccgcactta tgactgtctt ctttatcatg caactcgtag
    33361 gacaggtgcc ggcagcgctc tgggtcattt tcggcgagga ccgctttcgc tggagcgcga
    33421 cgatgatcgg cctgtcgctt gcggtattcg gaatcttgca cgccctcgct caagcct
//
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