LOCUS Exported 6258 bp ds-DNA circular SYN 03-NOV-2017
DEFINITION synthetic circular DNA
FEATURES Location/Qualifiers
source 1..6258
/organism="synthetic DNA construct"
/mol_type="other DNA"
polyA_signal 3046..3167
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(3174..3629)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 3656..3760
/gene="bla"
/label=AmpR promoter
promoter 3762..4119
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 3970..4105
/label=SV40 ori
/note="SV40 origin of replication"
CDS 4154..4948
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 5180..5227
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 5556..6144
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 tagttattac cggactcaga tctcgagacc tagaaaaaca tggagcaatc acaagtagca
61 atacagcagc taacaatgct gcttgtgcct ggctagaagc acaagaggag gaagaggtgg
121 gttttccagt cacacctcag gtacctttaa gaccaatgac ttacaaggca gctgtagatc
181 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caaagaagac
241 aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat tggcagaact
301 acacaccagg gccaggggtc agatatccac tgacctttgg atggtgctac aagctagtac
361 cagttgagcc agataaggta gaagaggcca ataaaggaga gaacaccagc ttgttacacc
421 ctgtgagcct gcatggaatg gatgaccctg agagagaagt gttagagtgg aggtttgaca
481 gccgcctagc atttcatcac gtggcccgag agctgcatcc ggagtacttc aagaactgct
541 gacatcgagc ttgctacaag ggactttccg ctggggactt tccagggagg cgtggcctgg
601 gcgggactgg ggagtggcga gccctcagat gctgcatata agcagctgct ttttgcctgt
661 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
721 ccactgctta agcctcaata aagctgatcc cccgggctgc aggaatttat gaaatccttt
781 atgggggacc cccccctttg tcaaccttgc tcaattcctt ctccccctcc gatcctaaga
841 ctgatttaca agcccgacta aaagggctgc aaggcgtgca ggcccaaatc tggacacccc
901 tggccgaatt gtaccggcca ggacatccac aaactagcca cccatttcag gtgggagact
961 ccgtgtacgt ccggcggcac cgctctcaag gattggagcc tcgttggaag ggaccttaca
1021 tcgtcctgct gaccacgccc accgccataa aggttgacgg gatcgccgcc tggattcacg
1081 catcgcacgc caaggcagcc ccaaaaaccc ctggaccaga aactcccaaa acctggaagc
1141 tccgccgttc ggagaaccct cttaagataa gactctcccg tgtctgactg ctaatccacc
1201 ttgtccctgt actaacccaa aatgaaactc ccaacaggaa tggtcatttt atgtagccta
1261 ataatagttc gggcagggtt tgacgacccc cgcaaggcta tcgcattagt acaaaaacaa
1321 catggtaaac catgcgaatg cagcggaggg caggtatccg aggccccacc gaactccatc
1381 caacaggtaa cttgcccagg caagacggcc tacttaatga ccaaccaaaa atggaaatgc
1441 agagtcactc caaaaaatct cacccctagc gggggagaac tccagaactg cccctgtaac
1501 actttccagg actcgatgca cagttcttgt tatactgaat accggcaatg cagggcgaat
1561 aataagacat actacacggc caccttgctt aaaatacggt ctgggagcct caacgaggta
1621 cagatattac aaaaccccaa tcagctccta cagtcccctt gtaggggctc tataaatcag
1681 cccgtttgct ggagtgccac agcccccatc catatctccg atggtggagg acccctcgat
1741 actaagagag tgtggacagt ccaaaaaagg ctagaacaaa ttcataaggc tatgcatcct
1801 gaacttcaat accacccctt agccctgccc aaagtcagag atgaccttag ccttgatgca
1861 cggacttttg atatcctgaa taccactttt aggttactcc agatgtccaa ttttagcctt
1921 gcccaagatt gttggctctg tttaaaacta ggtaccccta cccctcttgc gatacccact
1981 ccctctttaa cctactccct agcagactcc ctagcgaatg cctcctgtca gattatacct
2041 cccctcttgg ttcaaccgat gcagttctcc aactcgtcct gtttatcttc ccctttcatt
2101 aacgatacgg aacaaataga cttaggtgca gtcaccttta ctaactgcac ctctgtagcc
2161 aatgtcagta gtcctttatg tgccctaaac gggtcagtct tcctctgtgg aaataacatg
2221 gcatacacct atttacccca aaactggaca ggactttgcg tccaagcctc cctcctcccc
2281 gacattgaca tcatcccggg ggatgagcca gtccccattc ctgccattga tcattatata
2341 catagaccta aacgagctgt acagttcatc cctttactag ctggactggg aatcaccgca
2401 gcattcacca ccggagctac aggcctaggt gtctccgtca cccagtatac aaaattatcc
2461 catcagttaa tatctgatgt ccaagtctta tccggtacca tacaagattt acaagaccag
2521 gtagactcgt tagctgaagt agttctccaa aataggaggg gactggacct actaacggca
2581 gaacaaggag gaatttgttt agccttacaa gaaaaatgct gtttttatgc taacaagtca
2641 ggaattgtga gaaacaaaat aagaacccta caagaagaat tacaaaaacg cagggaaagc
2701 ctggcatcca accctctctg gaccgggctg cagggctttc ttccgtacct cctacctctc
2761 ctgggacccc tactcaccct cctactcata ctaaccattg ggccatgcgt tttcaatcga
2821 ttggtccaat ttgttaaaga caggatctca gtggtccagg ctctggtttt gactcagcaa
2881 tatcaccagc taaaacccat agagtacgag ccatgaagat ccaccggatc tagataactg
2941 atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac
3001 ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca
3061 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
3121 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttaacg cgtaaattgt
3181 aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa
3241 ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt
3301 gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa
3361 agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag
3421 ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt
3481 tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg
3541 agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc
3601 cgcgcttaat gcgccgctac agggcgcgtc aggtggcact tttcggggaa atgtgcgcgg
3661 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
3721 accctgataa atgcttcaat aatattgaaa aaggaagagt cctgaggcgg aaagaaccag
3781 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
3841 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
3901 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
3961 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
4021 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
4081 tagtgaggag gcttttttgg aggcctaggc ttttgcaaag atcgatcaag agacaggatg
4141 aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt
4201 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt
4261 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc
4321 cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc
4381 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga
4441 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat
4501 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca
4561 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga
4621 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc
4681 gagcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat
4741 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga
4801 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg
4861 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt
4921 ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa
4981 gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg
5041 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
5101 ctggagttct tcgcccaccc tagggggagg ctaactgaaa cacggaagga gacaataccg
5161 gaaggaaccc gcgctatgac ggcaataaaa agacagaata aaacgcacgg tgttgggtcg
5221 tttgttcata aacgcggggt tcggtcccag ggctggcact ctgtcgatac cccaccgaga
5281 ccccattggg gccaatacgc ccgcgtttct tccttttccc caccccaccc cccaagttcg
5341 ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag gccctgccat agcctcaggt
5401 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
5461 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
5521 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
5581 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
5641 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
5701 gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
5761 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
5821 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
5881 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
5941 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
6001 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
6061 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
6121 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
6181 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
6241 cgtattaccg ccatgcat
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