LOCUS Exported 6586 bp ds-DNA circular SYN 11-DEC-2017
DEFINITION synthetic circular DNA
FEATURES Location/Qualifiers
source 1..6586
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_feature 579..920
/label=MESV Psi
/note="packaging signal of murine embryonic stem cell
virus"
CDS 987..1403
/label=gag (truncated)
/note="truncated Moloney murine leukemia virus (MMLV) gag
gene lacking the start codon"
misc_feature 1447..1683
/label=Stuffer
promoter complement(1690..1904)
/label=H1 promoter
/note="human H1 RNA promoter"
promoter 1915..2414
/label=PGK promoter
/note="mouse phosphoglycerate kinase 1 promoter"
CDS 2439..3038
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
rep_origin complement(4033..4621)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(4792..5652)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(5653..5757)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat
61 ggaaaataca taactgagaa tagagaagtt cagatcaagg ttaggaacag agagacagca
121 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga
181 acagatggtc cccagatgcg gtcccgccct cagcagtttc tagagaacca tcagatgttt
241 ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc
301 gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc
361 ctcactcggc gcgccagtcc tccgatagac tgcgtcgccc gggtacccgt attcccaata
421 aagcctcttg ctgtttgcat ccgaatcgtg gactcgctga tccttgggag ggtctcctca
481 gattgattga ctgcccacct cgggggtctt tcatttggag gttccaccga gatttggaga
541 cccctgccca gggaccaccg acccccccgc cgggaggtaa gctggccagc ggtcgtttcg
601 tgtctgtctc tgtctttgtg cgtgtttgtg ccggcatcta atgtttgcgc ctgcgtctgt
661 actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac gagttctgaa
721 cacccggccg caaccctggg agacgtccca gggactttgg gggccgtttt tgtggcccga
781 cctgaggaag ggagtcgatg tggaatccga ccccgtcagg atatgtggtt ctggtaggag
841 acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tggaaccgaa
901 gccgcgcgtc ttgtctgctg cagcgctgca gcatcgttct gtgttgtctc tgtctgactg
961 tgtttctgta tttgtctgaa aattagggcc agactgttac cactccctta agtttgacct
1021 taggtcactg gaaagatgtc gagcggatcg ctcacaacca gtcggtagat gtcaagaaga
1081 gacgttgggt taccttctgc tctgcagaat ggccaacctt taacgtcgga tggccgcgag
1141 acggcacctt taaccgagac ctcatcaccc aggttaagat caaggtcttt tcacctggcc
1201 cgcatggaca cccagaccag gtcccctaca tcgtgacctg ggaagccttg gcttttgacc
1261 cccctccctg ggtcaagccc tttgtacacc ctaagcctcc gcctcctctt cctccatccg
1321 ccccgtctct cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc ctttatccag
1381 ccctcactcc ttctctaggc gccggaatta gatcgatctc tcgaggtcga cggtatcgat
1441 aagcttctga ataagccctc gtaatatatt ttcatgaaga atttaggtcc aaaaaaaaga
1501 tgggcattaa ttctagtcat ttaaaaaatt ctatagatca gaggttacat ggccaagatt
1561 gaaacttaga ggagtatagt tacataaaag aaggcaaaac gatgtataaa tgaaagaaat
1621 tgagatggtg cacgatgcac agttgaagtg aacttgcggg gtttttcagt atctacgatt
1681 catagatctg tggtctcata cagaacttat aagattccca aatccaaaga catttcacgt
1741 ttatggtgat ttcccagaac acatagcgac atgcaaatat tgcagggcgc cactcccctg
1801 tccctcacag ccatcttcct gccagggcgc acgcgcgctg ggtgttcccg cctagtgaca
1861 ctgggcccgc gattccttgg agcgggttga tgacgtcagc gttcgaattc taccgggtag
1921 gggaggcgct tttcccaagg cagtctggag catgcgcttt agcagccccg ctgggcactt
1981 ggcgctacac aagtggcctc tggcctcgca cacattccac atccaccggt aggcgccaac
2041 cggctccgtt ctttggtggc cccttcgcgc caccttctac tcctccccta gtcaggaagt
2101 tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca
2161 ctagtctcgt gcagatggac agcaccgctg agcaatggaa gcgggtaggc ctttggggca
2221 gcggccaata gcagctttgc tccttcgctt tctgggctca gaggctggga aggggtgggt
2281 ccgggggcgg gctcaggggc gggctcaggg gcggggcggg cgcccgaagg tcctccggag
2341 gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc tcttcctcat
2401 ctccgggcct ttcgacctgc agcccaagct agcttaccat gaccgagtac aagcccacgg
2461 tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg
2521 ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg
2581 agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg
2641 acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt
2701 tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac
2761 agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg
2821 tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag
2881 tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc
2941 tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac
3001 cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgacg cccgccccac gacccgcagc
3061 gcccgaccga aaggagcgca cgaccccatg catcgataaa ataaaagatt ttatttagtc
3121 tccagaaaaa ggggggaatg aaagacccca cctgtaggtt tggcaagcta gagaaccatc
3181 agatgtttcc agggtgcccc aaggacctga aatgaccctg tgccttattt gaactaacca
3241 atcagttcgc ttctcgcttc tgttcgcgcg cttctgctcc ccgagctcaa taaaagagcc
3301 cacaacccct cactcggcgc gccagtcctc cgatagactg cgtcgcccgg gtacccgtgt
3361 atccaataaa ccctcttgca gttgcatccg acttgtggtc tcgctgttcc ttgggagggt
3421 ctcctctgag tgattgacta cccgtcagcg ggggtctttc atgggtaaca gtttcttgaa
3481 gttggagaac aacattctga gggtaggagt cgaatattaa gtaatcctga ctcaattagc
3541 cactgttttg aatccacata ctccaatact cctgaaatag ttcattatgg acagcgcaga
3601 agagctgggg agaattaatt cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
3661 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
3721 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
3781 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
3841 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
3901 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
3961 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
4021 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
4081 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
4141 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
4201 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
4261 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
4321 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
4381 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
4441 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
4501 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
4561 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
4621 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
4681 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
4741 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
4801 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
4861 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
4921 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
4981 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
5041 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
5101 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
5161 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
5221 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
5281 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
5341 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
5401 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
5461 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
5521 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
5581 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
5641 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
5701 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
5761 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
5821 taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa
5881 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
5941 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta
6001 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag
6061 atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg
6121 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc
6181 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac
6241 ggcgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct
6301 gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc
6361 ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg
6421 gccacgatgc gtccggcgta gaggcgatta gtccaatttg ttaaagacag gatatcagtg
6481 gtccaggctc tagttttgac tcaacaatat caccagctga agcctataga gtacgagcca
6541 tagataaaat aaaagatttt atttagtctc cagaaaaagg ggggaa
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