LOCUS Exported 11625 bp ds-DNA circular SYN 10-DEC-2017
DEFINITION synthetic circular DNA
FEATURES Location/Qualifiers
source 1..11625
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 4..383
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 384..587
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
CDS 664..969
/codon_start=1
/label=tat
/translation="MEPVDPNLEPWKHPGSQPRTACNNCYCKKCCFHCYACFTRKGLGI
SYGRKKRRQRRRAPQDSQTHQASLSKQPASQSRGDPTGPTESKKKVERETETDPFD"
polyA_signal 976..1110
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
polyA_signal 2111..2245
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin 2968..4757
/label=oriP
/note="Epstein-Barr virus oriP replication origin (Yates et
al., 2000)"
CDS complement(5059..6984)
/codon_start=1
/product="Epstein-Barr nuclear antigen 1, also known as
EBNA-1"
/label=EBNA1
/note="crucial for latent viral infection, and for episomal
amplification of vectors containing the oriP origin (Okita
et al., 2013)"
/translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
GEEGQE"
promoter 7505..7609
/gene="bla"
/label=AmpR promoter
CDS 7610..8470
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 8641..9229
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
promoter 9660..9805
/label=HSV TK promoter
/note="herpes simplex virus thymidine kinase promoter"
CDS 9848..10885
/codon_start=1
/product="hygromycin B phosphotransferase"
/label=HygR
/note="confers resistance to hygromycin"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
MGEAN"
polyA_signal 10927..10975
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
1 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
241 atcaagtgta tcatatgcca agtccgcccc ctattgacgt caatgacggt aaatggcccg
301 cctggcatta tgcccagtac atgaccttac gggactttcc tacttggcag tacatctacg
361 tattagtcat cgctattacc atggtgatgc ggttttggca gtacaccaat gggcgtggat
421 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
481 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa taaccccgcc ccgttgacgc
541 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc
601 gtcagatctc tagaagctgg gtaccagctg ctagcaagct tgctagcggc cgctcgagcc
661 accatggagc cagtagatcc taatctagag ccctggaagc atccaggaag tcagcctagg
721 actgcttgta acaattgcta ttgtaaaaag tgttgctttc attgctacgc gtgtttcaca
781 agaaaaggct taggcatctc ctatggcagg aagaagcgga gacagcgacg aagagctcct
841 caggacagtc agactcatca agcttctcta tcaaagcaac ccgcctccca gtcccgaggg
901 gacccgacag ggcccacgga atcgaagaag aaggtggaga gagagacaga gacagatccg
961 ttcgattagc atgcagatcc agacatgata agatacattg atgagtttgg acaaaccaca
1021 actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt
1081 gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt
1141 caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt
1201 atggctgatt atgatccggc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac
1261 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag
1321 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg aggtcgactc
1381 tagaggatcg atccccgccc cggacgaact aaacctgact acgacatctc tgccccttct
1441 tcgcggggca gtgcatgtaa tcccttcagt tggttggtac aacttgccaa ctgggccctg
1501 ttccacatgt gacacggggg gggaccaaac acaaaggggt tctctgactg tagttgacat
1561 ccttataaat ggatgtgcac atttgccaac actgagtggc tttcatcctg gagcagactt
1621 tgcagtctgt ggactgcaac acaacattgc ctttatgtgt aactcttggc tgaagctctt
1681 acaccaatgc tgggggacat gtacctccca ggggcccagg aagactacgg gaggctacac
1741 caacgtcaat cagaggggcc tgtgtagcta ccgataagcg gaccctcaag agggcattag
1801 caatagtgtt tataaggccc ccttgttaac cctaaacggg tagcatatgc ttcccgggta
1861 gtagtatata ctatccagac taaccctaat tcaatagcat atgttaccca acgggaagca
1921 tatgctatcg aattagggtt agtaaaaggg tcctaaggaa cagcgatatc tcccacccca
1981 tgagctgtca cggttttatt tacatggggt caggattcca cgagggtagt gaaccatttt
2041 agtcacaagg gcagtggctg aagatcaagg agcgggcagg gaatctcctg aatctcggcc
2101 ggcaaggccg gatccagaca tgataagata cattgatgag tttggacaaa ccacaactag
2161 aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac
2221 cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt
2281 tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtatggc
2341 tgattatgat ccggctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg
2401 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt
2461 caggcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca tgaggtcgac tctagaggat
2521 cgatgccccg ccccggacga actaaacctg actacgacat ctctgcccct tcttcgcggg
2581 gcagtgcatg taatcccttc agttggttgg tacaacttgc caactgggcc ctgttccaca
2641 tgtgacacgg ggggggacca aacacaaagg ggttctctga ctgtagttga catccttata
2701 aatggatgtg cacatttgcc aacactgagt ggctttcatc ctggagcaga ctttgcagtc
2761 tgtggactgc aacacaacat tgcctttatg tgtaactctt ggctgaagct cttacaccaa
2821 tgctggggga catgtacctc ccaggggccc aggaagacta cgggaggcta caccaacgtc
2881 aatcagaggg gcctgtgtag ctaccgataa gcggaccctc aagagggcat tagcaatagt
2941 gtttataagg cccccttgtt aaccctaaac gggtagcata tgcttcccgg gtagtagtat
3001 atactatcca gactaaccct aattcaatag catatgttac ccaacgggaa gcatatgcta
3061 tcgaattagg gttagtaaaa gggtcctaag gaacagcgat atctcccacc ccatgagctg
3121 tcacggtttt atttacatgg ggtcaggatt ccacgagggt agtgaaccat tttagtcaca
3181 agggcagtgg ctgaagatca aggagcgggc agtgaactct cctgaatctt cgcctgcttc
3241 ttcattctcc ttcgtttagc taatagaata actgctgagt tgtgaacagt aaggtgtatg
3301 tgaggtgctc gaaaacaagg tttcaggtga cgcccccaga ataaaatttg gacggggggt
3361 tcagtggtgg cattgtgcta tgacaccaat ataaccctca caaacccctt gggcaataaa
3421 tactagtgta ggaatgaaac attctgaata tctttaacaa tagaaatcca tggggtgggg
3481 acaagccgta aagactggat gtccatctca cacgaattta tggctatggg caacacataa
3541 tcctagtgca atatgatact ggggttatta agatgtgtcc caggcaggga ccaagacagg
3601 tgaaccatgt tgttacactc tatttgtaac aaggggaaag agagtggacg ccgacagcag
3661 cggactccac tggttgtctc taacaccccc gaaaattaaa cggggctcca cgccaatggg
3721 gcccataaac aaagacaagt ggccactctt ttttttgaaa ttgtggagtg ggggcacgcg
3781 tcagccccca cacgccgccc tgcggttttg gactgtaaaa taagggtgta ataacttggc
3841 tgattgtaac cccgctaacc actgcggtca aaccacttgc ccacaaaacc actaatggca
3901 ccccggggaa tacctgcata agtaggtggg cgggccaaga taggggcgcg attgctgcga
3961 tctggaggac aaattacaca cacttgcgcc tgagcgccaa gcacagggtt gttggtcctc
4021 atattcacga ggtcgctgag agcacggtgg gctaatgttg ccatgggtag catatactac
4081 ccaaatatct ggatagcata tgctatccta atctatatct gggtagcata ggctatccta
4141 atctatatct gggtagcata tgctatccta atctatatct gggtagtata tgctatccta
4201 atttatatct gggtagcata ggctatccta atctatatct gggtagcata tgctatccta
4261 atctatatct gggtagtata tgctatccta atctgtatcc gggtagcata tgctatccta
4321 atagagatta gggtagtata tgctatccta atttatatct gggtagcata tactacccaa
4381 atatctggat agcatatgct atcctaatct atatctgggt agcatatgct atcctaatct
4441 atatctgggt agcataggct atcctaatct atatctgggt agcatatgct atcctaatct
4501 atatctgggt agtatatgct atcctaattt atatctgggt agcataggct atcctaatct
4561 atatctgggt agcatatgct atcctaatct atatctgggt agtatatgct atcctaatct
4621 gtatccgggt agcatatgct atcctcatgc atatacagtc agcatatgat acccagtagt
4681 agagtgggag tgctatcctt tgcatatgcc gccacctccc aagggggcgt gaattttcgc
4741 tgcttgtcct tttcctgctg gttgctccca ttcttaggtg aatttaagga ggccaggcta
4801 aagccgtcgc atgtctgatt gctcaccagg taaatgtcgc taatgttttc caacgcgaga
4861 aggtgttgag cgcggagctg agtgacgtga caacatgggt atgcccaatt gccccatgtt
4921 gggaggacga aaatggtgac aagacagatg gccagaaata caccaacagc acgcatgatg
4981 tctactgggg atttattctt tagtgcgggg gaatacacgg cttttaatac gattgagggc
5041 gtctcctaac aagttacatc actcctgccc ttcctcaccc tcatctccat cacctccttc
5101 atctccgtca tctccgtcat caccctccgc ggcagcccct tccaccatag gtggaaacca
5161 gggaggcaaa tctactccat cgtcaaagct gcacacagtc accctgatat tgcaggtagg
5221 agcgggcttt gtcataacaa ggtccttaat cgcatccttc aaaacctcag caaatatatg
5281 agtttgtaaa aagaccatga aataacagac aatggactcc cttagcgggc caggttgtgg
5341 gccgggtcca ggggccattc caaaggggag acgactcaat ggtgtaagac gacattgtgg
5401 aatagcaagg gcagttcctc gccttaggtt gtaaagggag gtcttactac ctccatatac
5461 gaacacaccg gcgacccaag ttccttcgtc ggtagtcctt tctacgtgac tcctagccag
5521 gagagctctt aaaccttctg caatgttctc aaatttcggg ttggaacctc cttgaccacg
5581 atgctttcca aaccaccctc cttttttgcg cctgcctcca tcaccctgac cccggggtcc
5641 agtgcttggg ccttctcctg ggtcatctgc ggggccctgc tctatcgctc ccgggggcac
5701 gtcaggctca ccatctgggc caccttcttg gtggtattca aaataatcgg cttcccctac
5761 agggtggaaa aatggccttc tacctggagg gggcctgcgc ggtggagacc cggatgatga
5821 tgactgacta ctgggactcc tgggcctctt ttctccacgt ccacgacctc tccccctggc
5881 tctttcacga cttccccccc tggctctttc acgtcctcta ccccggcggc ctccactacc
5941 tcctcgaccc cggcctccac tacctcctcg accccggcct ccactgcctc ctcgaccccg
6001 gcctccacct cctgctcctg cccctcctgc tcctgcccct cctcctgctc ctgcccctcc
6061 tgcccctcct gctcctgccc ctcctgcccc tcctgctcct gcccctcctg cccctcctgc
6121 tcctgcccct cctgcccctc ctcctgctcc tgcccctcct gcccctcctc ctgctcctgc
6181 ccctcctgcc cctcctgctc ctgcccctcc tgcccctcct gctcctgccc ctcctgcccc
6241 tcctgctcct gcccctcctg ctcctgcccc tcctgctcct gcccctcctg ctcctgcccc
6301 tcctgcccct cctgcccctc ctcctgctcc tgcccctcct gctcctgccc ctcctgcccc
6361 tcctgcccct cctgctcctg cccctcctcc tgctcctgcc cctcctgccc ctcctgcccc
6421 tcctcctgct cctgcccctc ctgcccctcc tcctgctcct gcccctcctc ctgctcctgc
6481 ccctcctgcc cctcctgccc ctcctcctgc tcctgcccct cctgcccctc ctcctgctcc
6541 tgcccctcct cctgctcctg cccctcctgc ccctcctgcc cctcctcctg ctcctgcccc
6601 tcctcctgct cctgcccctc ctgcccctcc tgcccctcct gcccctcctc ctgctcctgc
6661 ccctcctcct gctcctgccc ctcctgctcc tgcccctccc gctcctgctc ctgctcctgt
6721 tccaccgtgg gtccctttgc agccaatgca acttggacgt ttttggggtc tccggacacc
6781 atctctatgt cttggccctg atcctgagcc gcccggggct cctggtcttc cgcctcctcg
6841 tcctcgtcct cttccccgtc ctcgtccatg gttatcaccc cctcttcttt gaggtccact
6901 gccgccggag ccttctggtc cagatgtgtc tcccttctct cctaggccat ttccaggtcc
6961 tgtacctggc ccctcgtcag acatgattca cactaaaaga gatcaataga catctttatt
7021 agacgacgct cagtgaatac agggagtgca gactcctgcc ccctccaaca gcccccccac
7081 cctcatcccc ttcatggtcg ctgtcagaca gatccaggtc tgaaaattcc ccatcctccg
7141 aaccatcctc gtcctcatca ccaattactc gcagcccgga aaactcccgc tgaacatcct
7201 caagatttgc gtcctgagcc tcaagccagg cctcaaattc ctcgtccccc tttttgctgg
7261 acggtaggga tggggattct cgggacccct cctcttcctc ttcaaggtca ccagacagag
7321 atgctactgg ggcaacggaa gaaaagctgg gtgcggcctg tgaggatcag cttatcgatg
7381 ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt
7441 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
7501 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
7561 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
7621 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
7681 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
7741 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
7801 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc
7861 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
7921 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
7981 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
8041 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
8101 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat
8161 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
8221 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
8281 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
8341 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
8401 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
8461 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
8521 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
8581 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
8641 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
8701 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
8761 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
8821 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
8881 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
8941 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
9001 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
9061 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
9121 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
9181 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
9241 cgcggccttt ttacggttcc tggccttttg ctggccttga agctgtccct gatggtcgtc
9301 atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag
9361 aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc
9421 cagcgcgtcg gccccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat
9481 atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca
9541 attcttggag tggtgaatcc gttagcgagg tgccgccctg cttcatcccc gtggcccgtt
9601 gctcgcgttt gctggcggtg tccccggaag aaatatattt gcatgtcttt agttctatga
9661 tgacacaaac cccgcccagc gtcttgtcat tggcgaattc gaacacgcag atgcagtcgg
9721 ggcggcgcgg tccgaggtcc acttcgcata ttaaggtgac gcgtgtggcc tcgaacaccg
9781 agcgaccctg cagcgacccg cttaacagcg tcaacagcgt gccgcagatc ccggggggca
9841 atgagatatg aaaaagcctg aactcaccgc gacgtctgtc gagaagtttc tgatcgaaaa
9901 gttcgacagc gtctccgacc tgatgcagct ctcggagggc gaagaatctc gtgctttcag
9961 cttcgatgta ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg atggtttcta
10021 caaagatcgt tatgtttatc ggcactttgc atcggccgcg ctcccgattc cggaagtgct
10081 tgacattggg gaattcagcg agagcctgac ctattgcatc tcccgccgtg cacagggtgt
10141 cacgttgcaa gacctgcctg aaaccgaact gcccgctgtt ctgcagccgg tcgcggaggc
10201 catggatgcg atcgctgcgg ccgatcttag ccagacgagc gggttcggcc cattcggacc
10261 gcaaggaatc ggtcaataca ctacatggcg tgatttcata tgcgcgattg ctgatcccca
10321 tgtgtatcac tggcaaactg tgatggacga caccgtcagt gcgtccgtcg cgcaggctct
10381 cgatgagctg atgctttggg ccgaggactg ccccgaagtc cggcacctcg tgcacgcgga
10441 tttcggctcc aacaatgtcc tgacggacaa tggccgcata acagcggtca ttgactggag
10501 cgaggcgatg ttcggggatt cccaatacga ggtcgccaac atcttcttct ggaggccgtg
10561 gttggcttgt atggagcagc agacgcgcta cttcgagcgg aggcatccgg agcttgcagg
10621 atcgccgcgg ctccgggcgt atatgctccg cattggtctt gaccaactct atcagagctt
10681 ggttgacggc aatttcgatg atgcagcttg ggcgcagggt cgatgcgacg caatcgtccg
10741 atccggagcc gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg ccgtctggac
10801 cgatggctgt gtagaagtac tcgccgatag tggaaaccga cgccccagca ctcgtccgga
10861 tcgggagatg ggggaggcta actgaaacac ggaaggagac aataccggaa ggaacccgcg
10921 ctatgacggc aataaaaaga cagaataaaa cgcacgggtg ttgggtcgtt tgttcataaa
10981 cgcggggttc ggtcccaggg ctggcactct gtcgataccc caccgagacc ccattggggc
11041 caatacgccc gcgtttcttc cttttcccca ccccaccccc caagttcggg tgaaggccca
11101 gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag ccactggccc cgtgggttag
11161 ggacggggtc ccccatgggg aatggtttat ggttcgtggg ggttattatt ttgggcgttg
11221 cgtggggtca ggtccacgac tggactgagc agacagaccc atggtttttg gatggcctgg
11281 gcatggaccg catgtactgg cgcgacacga acaccgggcg tctgtggctg ccaaacaccc
11341 ccgaccccca aaaaccaccg cgcggatttc tggcgtgcca agctagtcga ccaattctca
11401 tgtttgacag cttatcatcg cagatccggg caacgttgtt gccattgctg caggcgcaga
11461 actggtaggt atggaagatc tatacattga atcaatattg gcaattagcc atattagtca
11521 ttggttatat agcataaatc aatattggct attggccatt gcatacgttg tatctatatc
11581 ataatatgta catttatatt ggctcatgtc caatatgacc gccat
//