LOCUS Exported 5874 bp ds-DNA circular SYN 14-SEP-2016
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS Untitled 11
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5874)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Wednesday, September 14, 2016 from SnapGene Viewer 3.2.1
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..5874
/organism="synthetic DNA construct"
/mol_type="other DNA"
LTR 414..589
/note="5' LTR (truncated)"
/note="truncated long terminal repeat from Moloney murine
sarcoma virus"
misc_feature 652..851
/note="MMLV Psi"
/note="packaging signal of Moloney murine leukemia virus
(MMLV)"
CDS 1052..1468
/note="gag (truncated)"
/note="truncated Moloney murine leukemia virus (MMLV) gag
gene lacking the start codon"
promoter 1503..1832
/note="SV40 promoter"
/note="SV40 enhancer and early promoter"
rep_origin 1683..1818
/note="SV40 ori"
/note="SV40 origin of replication"
CDS 1892..2686
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/note="NeoR/KanR"
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
LTR 2746..3339
/note="long terminal repeat from Moloney murine leukemia
virus"
misc_feature 3550..3690
/note="bom"
/note="basis of mobility region from pBR322"
rep_origin complement(3876..4464)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(4635..5495)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(5496..5600)
/gene="bla"
/note="AmpR promoter"
ORIGIN
1 tttgaaagac cccacccgta ggtggcaagc tagcttaagt aacgccactt tgcaaggcat
61 ggaaaaatac ataactgaga atagaaaagt tcagatcaag gtcaggaaca aagaaacagc
121 tgaataccaa acaggatatc tgtggtaagc ggttcctgcc ccggctcagg gccaagaaca
181 gatgagacag ctgagtgatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg
241 ctcggggcca agaacagatg gtccccagat gcggtccagc cctcagcagt ttctagtgaa
301 tcatcagatg tttccagggt gccccaagga cctgaaaatg accctgtacc ttatttgaac
361 taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc cgctctccga gctcaataaa
421 agagcccaca acccctcact cggcgcgcca gtcttccgat agactgcgtc gcccgggtac
481 ccgtattccc aataaagcct cttgctgttt gcatccgaat cgtggtctcg ctgttccttg
541 ggagggtctc ctctgagtga ttgactaccc acgacggggg tctttcattt gggggctcgt
601 ccgggatttg gagacccctg cccagggacc accgacccac caccgggagg taagctggcc
661 agcaacttat ctgtgtctgt ccgattgtct agtgtctatg tttgatgtta tgcgcctgcg
721 tctgtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa ctgacgagtt
781 ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc gtttttgtgg
841 cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg tggttctggt
901 aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt cggtttggaa
961 ccgaagccgc gcgtcttgtc tgctgcagcg ctgcagcatc gttctgtgtt gtctctgtct
1021 gactgtgttt ctgtatttgt ctgaaaatta gggccagact gttaccactc ccttaagttt
1081 gaccttaggt cactggaaag atgtcgagcg gatcgctcac aaccagtcgg tagatgtcaa
1141 gaagagacgt tgggttacct tctgctctgc agaatggcca acctttaacg tcggatggcc
1201 gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg tcttttcacc
1261 tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag ccttggcttt
1321 tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc ctcttcctcc
1381 atccgccccg tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat cctcccttta
1441 tccagccctc actccttctc taggcgccgg aattcgttaa ctcgaggatc cggctgtgga
1501 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
1561 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
1621 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
1681 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
1741 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
1801 gaggcttttt tggaggccta ggcttttgca aaaagcttgg gctgcaggtc gaggcggatc
1861 tgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg
1921 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg
1981 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa
2041 gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct
2101 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga
2161 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc
2221 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac
2281 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc
2341 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact
2401 gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga
2461 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg
2521 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga
2581 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga
2641 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg
2701 ttcgataaaa taaaagattt tatttagtct ccagaaaaag gggggaatga aagaccccac
2761 ctgtaggttt ggcaagctag cttaagtaac gccattttgc aaggcatgga aaaatacata
2821 actgagaata gagaagttca gatcaaggtc aggaacagat ggaacagctg aatatgggcc
2881 aaacaggata tctgtggtaa gcagttcctg ccccggctca gggccaagaa cagatggaac
2941 agctgaatat gggccaaaca ggatatctgt ggtaagcagt tcctgccccg gctcagggcc
3001 aagaacagat ggtccccaga tgcggtccag ccctcagcag tttctagaga accatcagat
3061 gtttccaggg tgccccaagg acctgaaatg accctgtgcc ttatttgaac taaccaatca
3121 gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga gctcaataaa agagcccaca
3181 acccctcact cggggcgcca gtcctccgat tgactgagtc gcccgggtac ccgtgtatcc
3241 aataaaccct cttgcagttg catccgactt gtggtctcgc tgttccttgg gagggtctcc
3301 tctgagtgat tgactacccg tcagcggggg tctttcattt gggggctcgt ccgggatcgg
3361 gagacccctg cccagggacc accgacccac caccgggagg taagctggct gcctcgcgcg
3421 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg
3481 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg
3541 gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata ctggcttaac
3601 tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac
3661 agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg
3721 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
3781 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
3841 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
3901 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
3961 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
4021 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
4081 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
4141 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
4201 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
4261 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
4321 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
4381 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
4441 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
4501 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
4561 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
4621 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
4681 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
4741 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
4801 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
4861 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
4921 aatagtttgc gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt
4981 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
5041 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
5101 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
5161 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
5221 cggcgaccga gttgctcttg cccggcgtca acacgggata ataccgcgcc acatagcaga
5281 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
5341 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
5401 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
5461 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
5521 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
5581 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
5641 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtcttcaa
5701 gaattgctag caattgctag caattgctag caattcatac cagatcaccg aaaactgtcc
5761 tccaaatgtg tccccctcac actcccaaat tcgcgggctt ctgcctctta gaccactcta
5821 ccctattccc cacactcacc ggagccaaag ccgcggccct tccgtttctt tgct
//