LOCUS Exported 8141 bp ds-DNA circular SYN 22-OCT-2017
DEFINITION synthetic circular DNA
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8141)
TITLE Direct Submission
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..8141
/organism="synthetic DNA construct"
/mol_type="other DNA"
LTR 234..414
/label=5' LTR (truncated)
misc_feature 458..583
/label=HIV-1 Psi
misc_feature 1076..1309
/label=RRE
misc_feature 1804..1920
/label=cPPT/CTS
promoter 2010..2213
/label=CMV promoter
misc_feature 2278..2320
/label=MCS
promoter 2353..2564
/label=EF-1-alpha core promoter
LTR 2577..2845
/label=5' LTR (truncated)
regulatory 2874..2879
CDS 2880..3575
/codon_start=1
/label=TurboRFP
/translation="MSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMKIKV
VEGGPLPFAFDILATSFMYGSKAFINHTQGIPDFFKQSFPEGFTWERITTYEDGGVLTA
TQDTSFQNGCIIYNVKINGVNFPSNGPVMQKKTRGWEANTEMLYPADGGLRGHSQMALK
LVGGGYLHCSFKTTYRSKKPAKNLKMPGFHFVDHRLERIKEADKETYVEQHEMAVAKYC
DLPSKLGHR"
CDS 3576..3629
/codon_start=1
/label=T2A
/translation="EGRGSLLTCGDVEENPGP"
CDS 3630..4229
/codon_start=1
/label=PuroR
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
misc_feature 4236..4824
/label=WPRE
LTR 4898..5131
/label=3' LTR (Delta-U3)
polyA_signal 5203..5324
/label=SV40 poly(A) signal
rep_origin 5343..5478
/label=SV40 ori
rep_origin complement(5918..6506)
/direction=LEFT
/label=ori
CDS complement(6677..7537)
/codon_start=1
/label=AmpR
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(7538..7642)
/label=AmpR promoter
ORIGIN
1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt
1921 atcgatacta gtattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
1981 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
2041 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
2101 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
2161 tgacgcaaat gggcggtagg cgtgtacggt gggaggttta tataagcaga gctcgtttag
2221 tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat agaagattct
2281 agagctagcg aattcgaatt taaatcggat ccgcggccgc aaggatctgc gatcgctccg
2341 gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg
2401 tcggcaattg aacgggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg
2461 tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg
2521 ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acagctgaag cttcgagggg
2581 ctcgcatctc tccttcacgc gcccgccgcc ctacctgagg ccgccatcca cgccggttga
2641 gtcgcgttct gccgcctccc gcctgtggtg cctcctgaac tgcgtccgcc gtctaggtaa
2701 gtttaaagct caggtcgaga ccgggccttt gtccggcgct cccttggagc ctacctagac
2761 tcagccggct ctccacgctt tgcctgaccc tgcttgctca actctacgtc tttgtttcgt
2821 tttctgttct gcgccgttac agatccaagc tgtgaccggc gcctacgcta gacgccacca
2881 tgagcgagct gatcaaggag aacatgcaca tgaagctgta catggagggc accgtgaaca
2941 accaccactt caagtgcaca tccgagggcg aaggcaagcc ctacgagggc acccagacca
3001 tgaagatcaa ggtggtcgag ggcggccctc tccccttcgc cttcgacatc ctggctacca
3061 gcttcatgta cggcagcaaa gccttcatca accacaccca gggcatcccc gacttcttta
3121 agcagtcctt ccctgagggc ttcacatggg agagaatcac cacatacgaa gacgggggcg
3181 tgctgaccgc tacccaggac accagcttcc agaacggctg catcatctac aacgtcaaga
3241 tcaacggggt gaacttccca tccaacggcc ctgtgatgca gaagaaaaca cgcggctggg
3301 aggccaacac cgagatgctg taccccgctg acggcggcct gagaggccac agccagatgg
3361 ccctgaagct cgtgggcggg ggctacctgc actgctcctt caagaccaca tacagatcca
3421 agaaacccgc taagaacctc aagatgcccg gcttccactt cgtggaccac agactggaaa
3481 gaatcaagga ggccgacaaa gagacctacg tcgagcagca cgagatggct gtggccaagt
3541 actgcgacct ccctagcaaa ctggggcaca gatgagaggg cagaggaagt ctgctaacat
3601 gcggtgacgt cgaggagaat cctggcccaa tgaccgagta caagcccacg gtgcgcctcg
3661 ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc
3721 ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag
3781 aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg
3841 ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga
3901 tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag
3961 gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct
4021 cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg
4081 ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct
4141 acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct
4201 ggtgcatgac ccgcaagccc ggtgcctgag tcgacaatca acctctggat tacaaaattt
4261 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
4321 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
4381 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
4441 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
4501 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
4561 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
4621 tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc
4681 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
4741 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
4801 tctccctttg ggccgcctcc ccgcctggta cctttaagac caatgactta caaggcagct
4861 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa
4921 cgaaaataag atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc
4981 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
5041 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
5101 cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct tattattcag
5161 tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt tattgcagct
5221 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
5281 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggctctag
5341 ctatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
5401 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
5461 gaggcttttt tggaggccta gacttttgca gagacggccc aaattcgtaa tcatggtcat
5521 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
5581 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
5641 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
5701 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
5761 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
5821 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
5881 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
5941 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
6001 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
6061 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
6121 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
6181 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
6241 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
6301 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
6361 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
6421 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
6481 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
6541 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
6601 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
6661 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
6721 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
6781 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
6841 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
6901 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
6961 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
7021 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
7081 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
7141 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
7201 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
7261 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
7321 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
7381 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
7441 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
7501 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
7561 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
7621 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa
7681 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg
7741 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
7801 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
7861 gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc
7921 atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt
7981 cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac
8041 gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt
8101 cccagtcacg acgttgtaaa acgacggcca gtgccaagct g
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