LOCUS Exported 9955 bp ds-DNA circular SYN 26-九月-2017
DEFINITION .
ACCESSION .
VERSION .
KEYWORDS FUGW
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9955)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported 2017年9月26日 from MiaoLingPlasmid
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..9955
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 238..617
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 619..817
/note="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
LTR 835..1015
/note="5' LTR (truncated)"
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 1062..1187
/note="HIV-1 Psi"
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1680..1913
/note="RRE"
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
misc_feature 2440..2557
/note="cPPT/CTS"
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 2622..3833
/note="UbC promoter"
/note="human ubiquitin C promoter"
CDS 3887..4606
/codon_start=1
/product="enhanced GFP"
/note="EGFP"
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
misc_feature 4647..5235
/note="WPRE"
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
LTR 5760..5940
/note="5' LTR (truncated)"
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
polyA_signal 5972..6196
/note="bGH poly(A) signal"
/note="bovine growth hormone polyadenylation signal"
rep_origin 6242..6670
/direction=RIGHT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 6684..7013
/note="SV40 promoter"
/note="SV40 enhancer and early promoter"
rep_origin 6864..6999
/note="SV40 ori"
/note="SV40 origin of replication"
promoter 7061..7108
/note="EM7 promoter"
/note="synthetic bacterial promoter "
CDS 7127..7501
/codon_start=1
/gene="Sh ble from Streptoalloteichus hindustanus"
/product="antibiotic-binding protein"
/note="Zeo"
/note="confers resistance to bleomycin, phleomycin, and
Zeocin(TM)"
/translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
EFALRDPAGNCVHFVAEEQD"
polyA_signal 7631..7752
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin complement(8203..8791)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(8962..9822)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(9823..9927)
/gene="bla"
/note="AmpR promoter"
ORIGIN
1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
2581 agatccagtt tggttaatta acccgtgtcg gctccagatc tggcctccgc gccgggtttt
2641 ggcgcctccc gcgggcgccc ccctcctcac ggcgagcgct gccacgtcag acgaagggcg
2701 cagcgagcgt cctgatcctt ccgcccggac gctcaggaca gcggcccgct gctcataaga
2761 ctcggcctta gaaccccagt atcagcagaa ggacatttta ggacgggact tgggtgactc
2821 tagggcactg gttttctttc cagagagcgg aacaggcgag gaaaagtagt cccttctcgg
2881 cgattctgcg gagggatctc cgtggggcgg tgaacgccga tgattatata aggacgcgcc
2941 gggtgtggca cagctagttc cgtcgcagcc gggatttggg tcgcggttct tgtttgtgga
3001 tcgctgtgat cgtcacttgg tgagtagcgg gctgctgggc tggccggggc tttcgtggcc
3061 gccgggccgc tcggtgggac ggaagcgtgt ggagagaccg ccaagggctg tagtctgggt
3121 ccgcgagcaa ggttgccctg aactgggggt tggggggagc gcagcaaaat ggcggctgtt
3181 cccgagtctt gaatggaaga cgcttgtgag gcgggctgtg aggtcgttga aacaaggtgg
3241 ggggcatggt gggcggcaag aacccaaggt cttgaggcct tcgctaatgc gggaaagctc
3301 ttattcgggt gagatgggct ggggcaccat ctggggaccc tgacgtgaag tttgtcactg
3361 actggagaac tcggtttgtc gtctgttgcg ggggcggcag ttatggcggt gccgttgggc
3421 agtgcacccg tacctttggg agcgcgcgcc ctcgtcgtgt cgtgacgtca cccgttctgt
3481 tggcttataa tgcagggtgg ggccacctgc cggtaggtgt gcggtaggct tttctccgtc
3541 gcaggacgca gggttcgggc ctagggtagg ctctcctgaa tcgacaggcg ccggacctct
3601 ggtgagggga gggataagtg aggcgtcagt ttctttggtc ggttttatgt acctatcttc
3661 ttaagtagct gaagctccgg ttttgaacta tgcgctcggg gttggcgagt gtgttttgtg
3721 aagtttttta ggcacctttt gaaatgtaat catttgggtc aatatgtaat tttcagtgtt
3781 agactagtaa attgtccgct aaattctggc cgtttttggc ttttttgtta gacgaagctt
3841 gggctgcagg tcgactctag aggatccccg ggtaccggtc gccaccatgg tgagcaaggg
3901 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
3961 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct
4021 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct
4081 gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt
4141 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
4201 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
4261 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
4321 ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa
4381 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca
4441 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca
4501 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
4561 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaaagcg gccgcgactc
4621 tagaattcga tatcaagctt atcgataatc aacctctgga ttacaaaatt tgtgaaagat
4681 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
4741 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
4801 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
4861 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
4921 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
4981 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
5041 aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
5101 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
5161 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
5221 gggccgcctc cccgcatcga taccgtcgac ctcgagacct agaaaaacat ggagcaatca
5281 caagtagcaa tacagcagct accaatgctg attgtgcctg gctagaagca caagaggagg
5341 aggaggtggg ttttccagtc acacctcagg tacctttaag accaatgact tacaaggcag
5401 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
5461 aacgaagaca agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt
5521 ggcagaacta cacaccaggg ccagggatca gatatccact gacctttgga tggtgctaca
5581 agctagtacc agttgagcaa gagaaggtag aagaagccaa tgaaggagag aacacccgct
5641 tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagta ttagagtgga
5701 ggtttgacag ccgcctagca tttcatcaca tggcccgaga gctgcatccg gactgtactg
5761 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
5821 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
5881 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
5941 gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt
6001 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
6061 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
6121 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
6181 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc
6241 cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
6301 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
6361 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
6421 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
6481 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
6541 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
6601 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
6661 aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
6721 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
6781 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
6841 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
6901 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg
6961 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc
7021 cgggagcttg tatatccatt ttcggatctg atcagcacgt gttgacaatt aatcatcggc
7081 atagtatatc ggcatagtat aatacgacaa ggtgaggaac taaaccatgg ccaagttgac
7141 cagtgccgtt ccggtgctca ccgcgcgcga cgtcgccgga gcggtcgagt tctggaccga
7201 ccggctcggg ttctcccggg acttcgtgga ggacgacttc gccggtgtgg tccgggacga
7261 cgtgaccctg ttcatcagcg cggtccagga ccaggtggtg ccggacaaca ccctggcctg
7321 ggtgtgggtg cgcggcctgg acgagctgta cgccgagtgg tcggaggtcg tgtccacgaa
7381 cttccgggac gcctccgggc cggccatgac cgagatcggc gagcagccgt gggggcggga
7441 gttcgccctg cgcgacccgg ccggcaactg cgtgcacttc gtggccgagg agcaggactg
7501 acacgtgcta cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
7561 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
7621 cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
7681 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
7741 caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg
7801 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
7861 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
7921 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
7981 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
8041 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
8101 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
8161 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
8221 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
8281 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
8341 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
8401 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
8461 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
8521 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
8581 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
8641 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
8701 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
8761 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
8821 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
8881 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
8941 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
9001 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
9061 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
9121 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
9181 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
9241 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
9301 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
9361 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
9421 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
9481 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
9541 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
9601 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
9661 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
9721 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
9781 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
9841 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
9901 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgac
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