LOCUS Exported 10668 bp ds-DNA circular SYN 27-AUG-2016
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS Untitled 30
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10668)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Saturday, August 27, 2016 from SnapGene Viewer 3.1.4
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..10668
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 921..1300
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 1331..1435
/gene="bla"
/note="AmpR promoter"
CDS 1436..2296
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 2467..3055
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
polyA_signal 3294..3428
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
promoter complement(3434..3629)
/note="SV40 promoter"
/note="SV40 early promoter"
rep_origin 3448..3583
/note="SV40 ori"
/note="SV40 origin of replication"
protein_bind 3688..3709
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 3724..3754
/note="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind 3762..3778
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 3786..3802
/note="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
polyA_site 4163..4218
/note="beta-globin poly(A) signal"
/note="rabbit beta-globin polyadenylation signal"
misc_feature 5117..5350
/note="RRE"
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS complement(5924..8935)
/codon_start=1
/gene="pol"
/product="pol protein from human immunodeficiency virus 1"
/note="HIV-1 pol"
/translation="FFREDLAFPQGKAREFSSEQTRANSPTRRELQVWGRDNNSLSEAG
ADRQGTVSFSFPQITLWQRPLVTIKIGGQLKEALLDTGADDTVLEEMNLPGRWKPKMIG
GIGGFIKVRQYDQILIEICGHKAIGTVLVGPTPVNIIGRNLLTQIGCTLNFPISPIETV
PVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPVFAIKKKD
STKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKQKKSVTVLDVGDAYFSVPLDKDFRK
YTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQCSMTKILEPFRKQNPDIVIYQYMD
DLYVGSDLEIGQHRTKIEELRQHLLRWGFTTPDKKHQKEPPFLWMGYELHPDKWTVQPI
VLPEKDSWTVNDIQKLVGKLNWASQIYAGIKVRQLCKLLRGTKALTEVVPLTEEAELEL
AENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMKGAHT
NDVKQLTEAVQKIATESIVIWGKTPKFKLPIQKETWEAWWTEYWQATWIPEWEFVNTPP
LVKLWYQLEKEPIIGAETFYVDGAANRETKLGKAGYVTDRGRQKVVPLTDTTNQKTELQ
AIHLALQDSGLEVNIVTDSQYALGIIQAQPDKSESELVSQIIEQLIKKEKVYLAWVPAH
KGIGGNEQVDKLVSAGIRKVLFLDGIDKAQEEHEKYHSNWRAMASDFNLPPVVAKEIVA
SCDKCQLKGEAMHGQVDCSPGIWQLDCTHLEGKVILVAVHVASGYIEAEVIPAETGQET
AYFLLKLAGRWPVKTVHTDNGSNFTSTTVKAACWWAGIKQEFGIPYNPQSQGVIESMNK
ELKKIIGQVRDQAEHLKTAVQMAVFIHNFKRKGGIGGYSAGERIVDIIATDIQTKELQK
QITKIQNFRVYYRDSRDPVWKGPAKLLWKGEGAVVIQDNSDIKVVPRRKAKIIRDYGKQ
MAGDDCVASRQDED"
misc_feature 6122..6239
/note="cPPT/CTS"
/note="central polypurine tract and central termination
sequence of HIV-1"
CDS complement(8728..10230)
/codon_start=1
/gene="gag"
/product="gag protein from human immunodeficiency virus 1"
/note="HIV-1 gag"
/translation="MGARASVLSGGELDRWEKIRLRPGGKKKYKLKHIVWASRELERFA
VNPGLLETSEGCRQILGQLQPSLQTGSEELRSLYNTVATLYCVHQRIEIKDTKEALDKI
EEEQNKSKKKAQQAAADTGHSNQVSQNYPIVQNIQGQMVHQAISPRTLNAWVKVVEEKA
FSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRVHPVHAGP
IAPGQMREPRGSDIAGTTSTLQEQIGWMTHNPPIPVGEIYKRWIILGLNKIVRMYSPTS
ILDIRQGPKEPFRDYVDRFYKTLRAEQASQEVKNWMTETLLVQNANPDCKTILKALGPG
ATLEEMMTACQGVGGPGHKARVLAEAMSQVTNPATIMIQKGNFRNQRKTVKCFNCGKEG
HIAKNCRAPRKKGCWKCGKEGHQMKDCTERQANFLGKIWPSHKGRPGNFLQSRPEPTAP
PEESFRFGEETTTPSQKQEPIDKELYPLASLRSLFGSDPSSQ"
intron 10336..684
/note="chimeric intron"
/note="chimera between introns from chicken beta-actin and
rabbit beta-globin"
ORIGIN
1 gcggctcgcc gcgcctcgac agccgccggc gctccggggg ccgccgcgcc cctcccccga
61 gccctccccg gcccgaggcg gccccgcccc gcccggcacc cccacctgcc gccacccccc
121 gcccggcacg gcgagccccg cgccacgccc cgtacggagc cccgcacccg aagccgggcc
181 gtgctcagca actcggggag gggggtgcag ggggggttgc agcccgaccg acgcgcccac
241 accccctgct caccccccca cgcacacacc ccgcacgcag cctttgttcc cctcgcagcc
301 ccccccgcac cgcggggcac cgcccccggc cgcgctcccc tcgcgcacac tgcggagcgc
361 acaaagcccc gcgccgcgcc cgcagcgctc acagccgccg ggcagcgcgg agccgcacgc
421 ggcgctcccc acgcacacac acacgcacgc accccccgag ccgctccccc cgcacaaagg
481 gccctcccgg agcccctcaa ggctttcacg cagccacaga aaagaaacaa gccgtcatta
541 aaccaagcgc taattacagc ccggaggaga agggccgtcc cgcccgctca cctgtgggag
601 taacgcggtc agtcagagcc ggggcgggcg gcgcgaggcg gcggcggagc ggggcacggg
661 gcgaaggcag cgcgcagcga ctcccgcccg ccgcgcgctt cgctttttat agggccgccg
721 ccgccgccgc ctcgccataa aaggaaactt tcggagcgcg ccgctctgat tggctgccgc
781 cgcacctctc cgcctcgccc cccccccccc cccgccccca tcgctgcaca aaataattaa
841 aaaataaata aatacaaaat tgggggtggg gagggggggg agatggggag agtgaagcag
901 aacgtggggc tcacctcgac catggtaata gcgatgacta atacgtagat gtactgccaa
961 gtaggaaagt cccataaggt catgtactgg gcataatgcc aggcgggcca tttaccgtca
1021 ttgacgtcaa tagggggcgt acttggcata tgatacactt gatgtactgc caagtgggca
1081 gtttaccgta aatactccac ccattgacgt caatggaaag tccctattgg cgttactatg
1141 ggaacatacg tcattattga cgtcaatggg cgggggtcgt tgggcggtca gccaggcggg
1201 ccatttaccg taagttatgt aacgcggaac tccatatatg ggctatgaac taatgacccc
1261 gtaattgatt actattaata actagtcaat aatcaatgtc gacccaggtg gcacttttcg
1321 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
1381 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
1441 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
1501 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
1561 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
1621 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
1681 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
1741 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
1801 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
1861 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
1921 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
1981 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
2041 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
2101 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
2161 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
2221 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
2281 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
2341 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
2401 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
2461 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
2521 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
2581 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
2641 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
2701 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
2761 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
2821 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
2881 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
2941 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
3001 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
3061 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
3121 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
3181 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
3241 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gcggatccag
3301 acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat
3361 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata
3421 aacaagttag ctttttgcaa aagcctaggc ctccaaaaaa gcctcctcac tacttctgga
3481 atagctcaga ggccgaggcg gcctcggcct ctgcataaat aaaaaaaatt agtcagccat
3541 ggggcggaga atgggcggaa ctgggcggag ttaggggcgg gatgggcgga gttaggggcg
3601 ggactatggt tgctgactaa ttgagatgcg gatccgctgg cacgacaggt ttcccgactg
3661 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
3721 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
3781 tcacacagga aacagctatg accatgatta cgccaagctt gggctgcagg tcgagggatc
3841 tccataagag aagagggaca gctatgactg ggagtagtca ggagaggagg aaaaatctgg
3901 ctagtaaaac atgtaaggaa aattttaggg atgttaaaga aaaaaataac acaaaacaaa
3961 atataaaaaa aatctaacct caagtcaagg cttttctatg gaataaggaa tggacagcag
4021 ggggctgttt catatactga tgacctcttt atagccaacc tttgttcatg gcagccagca
4081 tatgggcata tgttgccaaa ctctaaacca aatactcatt ctgatgtttt aaatgatttg
4141 ccctcccata tgtccttccg agtgagagac acaaaaaatt ccaacacact attgcaatga
4201 aaataaattt cctttattag ccagaagtca gatgctcaag gggcttcatg atgtccccat
4261 aatttttggc agagggaaaa agatctgcta gctatagttc tagaggtacc ggttgtttcg
4321 agcttatagc aaaatccttt ccaagccctg tcttattctt ctaggtatgt ggcgaatagc
4381 tctacaagct ccttgtacta cttctataac cctatctgtc ccctcagcta ctgctatggc
4441 tgtggcattg agcaagctaa cagcactatt ctttagctcc tgactccaat attgtaggag
4501 attccaccaa tatttgaggg cttcccaccc cctgcgtccc agaagttcca caatcctcgt
4561 tacaatcaag agtaagtctc tcaagcggtg gtagctgaag aggcacaggc tccgcagatc
4621 gtcccagata agtgccaagg atccgttcac taatcgaatg gatctgtctc tgtctctctc
4681 tccaccttct tcttctattc cttcgggcct gtcgggtccc ctcggggttg ggaggtgggt
4741 ctgaaacgat aatggtgaat atccctgcct aactctattc actatagaaa gtacagcaaa
4801 aactattctt aaacctacca agcctcctac tatcattatg aataatttta tataccacag
4861 ccaatttgtt atgttaaacc aattccacaa acttgcccat ttatctaatt ccaataattc
4921 ttgttcattc ttttcttgct ggttttgcga ttcttcaatt aaggagtgta ttaagcttgt
4981 gtaattgtta atttctctgt cccactccat ccaggtcgtg tgattccaaa tctgttccag
5041 agatttatta ctccaactag cattccaagg cacagcagtg gtgcaaatga gttttccaga
5101 gcaaccccaa atccccagga gctgttgatc ctttaggtat ctttccacag ccaggattct
5161 tgcctggagc tgcttgatgc cccagactgt gagttgcaac agatgctgtt gcgcctcaat
5221 agccctcagc aaattgttct gctgctgcac tataccagac aataattgtc tggcctgtac
5281 cgtcagcgtc attgacgctg cgcccatagt gcttcctgct gctcccaaga acccaaggaa
5341 caaagctcct attcccactg ctcttttttc tctctgcacc actcttctct ttgccttggt
5401 gggtgctact cctaatggtt caatttttac tactttatat ttatataatt cacttctcca
5461 attgtccctc atatctcctc ctccaggtct gaagatcagc ggccattttc ctatattcta
5521 tgattactat ggaccacaca actattgcta ttattattgc tactactaat gctactattg
5581 ctactattat aggttgcatt acatgtacta cttactgctt tgatagagaa gcttgatgag
5641 tctgactgtt ctgatgagct cttcgtcgct gtctccgctt cttcctgcca taggagatgc
5701 ctaaggcttt tgtcatgaaa caaacttggc aatgaaagca acacttttta caatagcaat
5761 tggtacaagc agttttaggc tgacttcctg gatgcttcca gggctctagt ctaggatcta
5821 ctggctccat ttcttgctct cctctgtcga gtaacgccta ttctgctatg tcgacaccca
5881 attctgaaat ggataaacag cagttgttgc agaattccat gtgttaatcc tcatcctgtc
5941 tacttgccac acaatcatca cctgccatct gttttccata atccctgatg atctttgctt
6001 ttcttcttgg cactactttt atgtcactat tatcttgtat tactactgcc ccttcacctt
6061 tccagaggag ctttgctggt cctttccaaa ctggatctct gctgtccctg taataaaccc
6121 gaaaattttg aatttttgta atttgttttt gtaattcttt agtttgtatg tctgttgcta
6181 ttatgtctac tattctttcc cctgcactgt accccccaat cccccctttt cttttaaaat
6241 tgtggatgaa tactgccatt tgtactgctg tcttaagatg ttcagcctga tctcttacct
6301 gtcctataat tttctttaat tctttattca tagattctat tactccttga ctttggggat
6361 tgtagggaat gccaaattcc tgcttgatcc ccgcccacca acaggcggcc ttaactgtag
6421 tactggtgaa attgctgcca ttgtctgtat gtactgtttt tactggccat cttcctgcta
6481 attttaagag gaagtatgct gtttcttgcc ctgtctctgc tggaattact tctgcttcta
6541 tatatccact ggctacatga actgctacca agataacttt tccttctaaa tgtgtacaat
6601 ctagctgcca tattcctggg ctacagtcta cttgtccatg catggcttcc ccttttagct
6661 gacatttatc acagctggct actatttctt ttgctactac aggtggtagg ttaaaatcac
6721 tagccattgc tctccaatta ctgtgatatt tctcatgttc ttcttgggcc ttatctattc
6781 catctaaaaa tagtactttc ctgattccag cactgaccaa tttatctact tgttcatttc
6841 ctccaattcc tttgtgtgct ggtacccatg ccaggtagac tttttccttt tttattaact
6901 gctctattat ttgactgact aactctgatt cactcttatc tggttgtgct tgaatgattc
6961 ccaatgcata ttgtgagtct gtcactatgt ttacttctaa tcccgaatcc tgcaaagcta
7021 gatgaattgc ttgtaactca gtcttctgat ttgttgtgtc cgttaggggg acaacttttt
7081 gtcttcctct gtcagttaca tatcctgctt ttcctaattt agtttcccta ttggctgccc
7141 catctacata gaaagtttct gctcctatta tgggttcttt ctctaactgg taccataact
7201 tcactaaggg aggggtattg acaaactccc actcaggaat ccaggtggct tgccaatact
7261 ctgtccacca tgcttcccat gtttcctttt gtatgggtaa tttaaattta ggagtctttc
7321 cccatattac tatgctttct gtggctattt tttgtactgc ctctgttaat tgtttcacat
7381 cattagtgtg ggcacccttc attcttgcat actttcctgt tttcagattt ttaaatggct
7441 cttgataaat ttgatatgtc cattggcctt gcccctgctt ctgtatttct gctattaagt
7501 cttttgatgg gtcataatac actccatgta ccggttcttt tagaatctcc ctgttttctg
7561 ccagttctag ctctgcttct tctgttagtg gtactacttc tgttagtgct ttggttcccc
7621 taagaagttt acataattgc cttactttaa tccctgcata aatctgactt gcccaattca
7681 attttcccac taatttctgt atgtcattga cagtccagct gtccttttct ggcagcacta
7741 taggctgtac tgtccattta tcaggatgga gttcataacc catccaaagg aatggaggtt
7801 ctttctgatg ttttttgtct ggtgtggtaa atccccacct caacagatgt tgtctcagtt
7861 cctctatttt tgttctatgc tgccctattt ctaagtcaga tcctacatac aaatcatcca
7921 tgtattgata gatgactatg tctggatttt gttttctaaa aggctctaag atttttgtca
7981 tgctacactg gaatattgct ggtgatcctt tccatccctg tggaagcaca ttgtactgat
8041 atctaatccc tggtgtctca ttgtttatac taggtatggt aaatgcagta tacttcctga
8101 agtctttatc taagggaact gaaaaatatg catcgcccac atccagtact gttactgatt
8161 ttttctgttt taaccctgca ggatgtggta ttcctaattg aacttcccag aaatcttgag
8221 ttctcttatt aagttctctg aaatctacta attttctcca tttagtactg tcttttttct
8281 ttatggcaaa tactggagta ttgtatggat tttcaggccc aatttttgaa atttttcctt
8341 ccttttccat ttctgtacaa atttctacta atgcttttat tttttcttct gtcaatggcc
8401 attgtttaac ttttgggcca tccattcctg gctttaattt tactggtaca gtctcaatag
8461 gactaatggg aaaatttaaa gtgcagccaa tctgagtcaa cagatttctt ccaattatgt
8521 tgacaggtgt aggtcctact aatactgtac ctatagcttt atgtccgcag atttctatga
8581 gtatctgatc atactgtctt actttgataa aacctccaat tccccctatc atttttggtt
8641 tccatcttcc tggcaaattc atttcttcta atactgtatc atctgctcct gtatctaata
8701 gagcttcctt taattgcccc cctatcttta ttgtgacgag gggtcgctgc caaagagtga
8761 tctgagggaa gctaaaggat acagttcctt gtctatcggc tcctgcttct gagagggagt
8821 tgttgtctct tccccaaacc tgaagctctc ttctggtggg gctgttggct ctggtctgct
8881 ctgaagaaaa ttccctggcc ttcccttgtg ggaaggccag atcttcccta aaaaattagc
8941 ctgtctctca gtacaatctt tcatttggtg tccttccttt ccacatttcc aacagccctt
9001 tttcctaggg gccctgcaat ttttggctat gtgcccttct ttgccacaat tgaaacactt
9061 aacagtcttt ctttggttcc taaaattgcc tttctgtatc attatggtag ctggatttgt
9121 tacttggctc attgcttcag ccaaaactct tgctttatgg ccgggtcccc ccactccctg
9181 acatgctgtc atcatttctt ctagtgtcgc tcctggtccc aatgctttta aaatagtctt
9241 acaatctggg ttcgcatttt ggaccaacaa ggtttctgtc atccaatttt ttacctcttg
9301 tgaagcttgc tcggctctta gagttttata gaatcggtct acatagtctc taaagggttc
9361 ctttggtcct tgtcttatgt ccagaatgct ggtagggcta tacattctta ctattttatt
9421 taatcccagg attatccatc ttttatagat ttctcctact gggataggtg gattatgtgt
9481 catccatcct atttgttcct gaagggtact agtagttcct gctatgtcac ttccccttgg
9541 ttctctcatc tggcctggtg caataggccc tgcatgcact ggatgcactc tatcccattc
9601 tgcagcttcc tcattgatgg tctcttttaa catttgcatg gctgcttgat gtccccccac
9661 tgtgtttagc atggtgttta aatcttgtgg ggtggctcct tctgataatg ctgaaaacat
9721 gggtatcact tctgggctga aagccttctc ttctactact tttacccatg catttaaagt
9781 tctaggtgat atggcctgat gtaccatttg cccctggatg ttctgcacta tagggtaatt
9841 ttggctgacc tgattgctgt gtcctgtgtc agctgctgct tgctgtgctt ttttcttact
9901 tttgttttgc tcttcctcta tcttgtctaa agcttccttg gtgtctttta tctctatcct
9961 ttgatgcaca caatagaggg ttgctactgt attatataat gatctaagtt cttctgatcc
10021 tgtctgaagg gatggttgta gctgtcccag tatttgtcta cagccttctg atgtttctaa
10081 caggccagga ttaactgcga atcgttctag ctccctgctt gcccatacta tatgttttaa
10141 tttatatttt ttctttcccc ctggccttaa ccgaattttt tcccatcgat ctaattctcc
10201 cccgcttaat actgacgctc tcgcacccat ctctcaccag tcgccgcccc tcgcctcttg
10261 ccgtgcgcgt caacgcggcc cgaattcttt gccaaaatga tgagacagca caataaccag
10321 cacgttgccc aggagctgta ggaaaaagaa gaaggcatga acatggttag cagaggctct
10381 agagccgccg gtcacacgcc agaagccgaa ccccgccctg ccccgtcccc cccgaaggca
10441 gccgtccccc cgcggacagc cccgaggctg gagagggaga aggggacggc ggcgcggcga
10501 cgcacgaagg ccctccccgc ccatttcctt cctgccggcg ccgcaccgct tcgccccgcg
10561 cccgctagag ggggtgcggc ggcgcctccc agatttcggc tccgcacaga tttgggacaa
10621 aggaagtccc tgcgccctct cgcacgatta ccataaaagg caatggct
//