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产品名称：pLVX-TRE3G
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产品展商：XYbscience
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简单介绍

pLVX-TRE3G菌株发货前均经过严格的多重验证，如存在质量问题，请在收到产品的三个月内通知我司。pLVX-TRE3G收到质粒后请短暂离心，加入20μl ddH2O溶解质粒，取2μl转化至对应感受态中，挑取单克��重新提取质粒后使用。

产品描述

pLVX-TRE3G

产品信息
基本信息
质粒简介
质粒图谱
质粒序列

产品信息

产品货号	产品名称	产品规格	优惠价
XY0267	pLVX-TRE3G	20μl	¥请询价

使用说明

信裕质粒平台的各批次质粒菌株发货前均经过严格的多重验证，如存在质量问题，请在收到产品的三个月内通知我司。收到质粒后请短暂离心，加入20μl ddH2O溶解质粒，取2μl转化至对应感受态中，挑取单克隆重新提取质粒后使用。如需获取其他详细信息请登录我司官网查询。

基本信息

启动子:	TRE3GV，PGK
复制子:	pUC ori
终止子:	SV40 poly(A) signal
质粒分类:	病毒系列，慢病毒克隆载体
质粒大小:	7819bp
原核抗性:	Amp
筛选标记:	Puro
克隆菌株:	Stbl3
培养条件:	37℃，有氧 LB
表达宿主:	哺乳细胞
诱导方式:	四环素诱导
5'测序引物:	M13R:CAGGAAACAGCTATGACC
3'测序引物:	根据序列设计引物

质粒简介

When used with any Lenti-X Tet-On 3G or Tet-Express inducible expression system, the pLVX-TRE3G Vector Set allows for lentiviral delivery and inducible expression of your gene of interest in a wide variety of mammalian cells. Target cells that contain a gene of interest under the control of a PTRE3GV promoter will express high levels of your gene of interest, when cultured in the presence of doxycycline (when used with the pLVX-Tet3G Vector and a Tet-On 3G system) or in the presence of the Tet-Express tetracycline transactivator (when used with Tet-Express Systems).

质粒图谱

质粒序列

LOCUS Exported 7819 bp ds-DNA circular SYN 29-JAN-2014

DEFINITION Lentiviral vector for doxycycline-inducible expression of a gene in

cells containing the Tet-On 3G transactivator protein.

ACCESSION .

VERSION .

KEYWORDS pLVX-TRE3G

SOURCE synthetic DNA construct

ORGANISM synthetic DNA construct

REFERENCE 1 (bases 1 to 7819)

AUTHORS Clontech

TITLE Direct Submission

JOURNAL Exported Thursday, September 8, 2016 from SnapGene Viewer 3.1.4

http://www.miaolingbio.com

FEATURES Location/Qualifiers

source 1..7819

/organism="synthetic DNA construct"

/lab_host="Mammalian Cells"

/mol_type="other DNA"

LTR 1..634

/note="5' LTR"

/note="5' long terminal repeat (LTR) from HIV-1"

misc_feature 681..806

/note="HIV-1 psi"

/note="packaging signal of human immunodeficiency virus

type 1"

misc_feature 1303..1536

/note="RRE"

/note="The Rev response element (RRE) of HIV-1 allows for

Rev-dependent mRNA export from the nucleus to the

cytoplasm."

misc_feature 2028..2144

/note="cPPT/CTS"

/note="central polypurine tract and central termination

sequence of HIV-1 (lacking the first T)"

promoter 2209..2555

/note="TRE3GV promoter"

/note="3rd-generation Tet-responsive promoter that can be

activated by binding of Tet-On 3G; optimized for retroviral

and lentiviral vectors"

protein_bind 2214..2232

/gene="tetO"