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产品名称：pLVX-TRE3G-Zsgreen1
产品型号：
产品展商：XYbscience
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简单介绍

pLVX-TRE3G-Zsgreen1菌株发货前均经过严格的多重验证，如存在质量问题，请在收到产品的三个月内通知我司。pLVX-TRE3G-Zsgreen1收到质粒后��短暂离心，加入20μl ddH2O溶解质粒，取2μl转化至对应感受态中，挑取单克隆重新提取质粒后使用。

产品描述

pLVX-TRE3G-Zsgreen1

产品信息
基本信息
质粒简介
质粒图谱
质粒序列

产品信息

产品货号	产品名称	产品规格	优惠价
XY0440	pLVX-TRE3G-Zsgreen1	20μl	¥请询价

使用说明

信裕质粒平台的各批次质粒菌株发货前均经过严格的多重验证，如存在质量问题，请在收到产品的三个月内通知我司。收到质粒后请短暂离心，加入20μl ddH2O溶解质粒，取2μl转化至对应感受态中，挑取单克隆重新提取质粒后使用。如需获取其他详细信息请登录我司官网查询。

基本信息

启动子:	TRE3GV，PGK
复制子:	pUC ori
终止子:	SV40 poly(A) signal
质粒分类:	病毒系列，慢病毒克隆载体
质粒大小:	9100bp
原核抗性:	Amp
筛选标记:	Puro
克隆菌株:	Stbl3
培养条件:	37℃，有氧 LB
表达宿主:	哺乳细胞
诱导方式:	四环素诱导
5'测序引物:	M13R:CAGGAAACAGCTATGACC
3'测序引物:	根据序列设计引物

质粒简介

The inducible promoter PTRE3G provides for very low basal expression and high maximal expression after induction. It consists of 7 repeats of a 19 bp tet operator sequence located upstream of a minimal CMV promoter. PTRE3GV is a version of PTRE3G that was modified at Clontech for higher performance in lentiviruses and retroviruses. In the presence of Dox, Tet-On 3G binds specifically to PTRE3GV and activates transcription of the downstream GOI. PTRE3GV lacks binding sites for endogenous mammalian transcription factors, so it is virtually silent in the absence of induction. pLVX-TRE3G-ZsGreen1 is an HIV-1-based, inducible lentiviral expression vector that allows the simultaneous expression of ZsGreen1 and your protein of interest in virtually any mammalian cell type, including primary cells.

质粒图谱

质粒序列

LOCUS Exported 9100 bp ds-DNA circular SYN 27-AUG-2016

DEFINITION synthetic circular DNA

ACCESSION .

VERSION .

KEYWORDS Untitled 35

SOURCE synthetic DNA construct

ORGANISM synthetic DNA construct

REFERENCE 1 (bases 1 to 9100)

AUTHORS .

TITLE Direct Submission

JOURNAL Exported Saturday, August 27, 2016 from SnapGene Viewer 3.1.4

http://www.miaolingbio.com

FEATURES Location/Qualifiers

source 1..9100

/organism="synthetic DNA construct"

/mol_type="other DNA"

LTR 1..634

/note="3' LTR"

/note="3' long terminal repeat (LTR) from HIV-1"

misc_feature 681..806

/note="HIV-1 Psi"

/note="packaging signal of human immunodeficiency virus

type 1"

misc_feature 1303..1536

/note="RRE"

/note="The Rev response element (RRE) of HIV-1 allows for

Rev-dependent mRNA export from the nucleus to the

cytoplasm."

misc_feature 2028..2143

/note="cPPT/CTS"

/note="central polypurine tract and central termination

sequence of HIV-1 (lacking the first T)"

promoter 2206..2555

/note="TRE3GV promoter"

/note="3rd-generation Tet-responsive promoter that can be

activated by binding of Tet-On(R) 3G, optimized for

retroviral and lentiviral vectors"

protein_bind 2214..2232

/gene="tetO"