LOCUS Exported 6606 bp ds-DNA circular SYN 30-AUG-2016
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS Untitled 3
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6606)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Tuesday, August 30, 2016 from SnapGene Viewer 3.1.4
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..6606
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 133..336
/note="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
promoter 1479..1682
/note="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
promoter 1839..2050
/note="EF-1-alpha core promoter"
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 2063..2331
/note="5' LTR (truncated)"
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
CDS 2358..2957
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/note="PuroR"
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
misc_feature 2964..3552
/note="WPRE"
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
CDS complement(3435..3446)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/note="Factor Xa site"
/translation="IEGR"
polyA_signal 3890..4011
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin 4030..4165
/note="SV40 ori"
/note="SV40 origin of replication"
primer_bind complement(4203..4219)
/note="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 4227..4243
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(4251..4281)
/note="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind 4296..4317
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(4605..5193)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(5364..6224)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(6225..6329)
/gene="bla"
/note="AmpR promoter"
ORIGIN
1 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
61 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
121 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
181 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
241 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
301 aggcgtgtac ggtgggaggt ctatataagc agagcttgtg aaacttcgag gagtctcttt
361 gttgaggact tttgagttct cccttgaggc tcccacagat acaataaata tttgagattg
421 aaccctgtcg agtatctgtg taatcttttt tacctgtgag gtctcggaat ccgggccgag
481 aacttcgcag ttggcgcccg aacagggact tgattgagag tgattgagga agtgaagcta
541 gagcaataga aagctgttaa gcagaactcc tgctgaccta aatagggaag cagtagcaga
601 cgctgctaac agtgagtatc tctagtgaag cagactcgag ctcataatca agtcattgtt
661 taaaggccca gataaattac atctggtgac tcttcgcgga ccttcaagcc aggagattcg
721 ccgagggaca gtcaacaagg taggagagat tctacagcaa catggggaat ggacaggggc
781 gagattggaa aatggccatt aagagatgta gtaatgttgc tgtaggagta ggggggaaga
841 gtaaaaaatt tggagaaggg aatttcagat gggccattag aatggctaat gtatctacag
901 gacgagaacc tggtgatata ccagagactt tagatcaact aaggttggtt atttgcgatt
961 tacaagaaag aagagaaaaa tttggatcta gcaaagaaat tgatatggca attcctgcat
1021 tgaggagaaa tggtaggcaa tgtggcatgt ctgaaaaaga ggaggaatga tgaagtatct
1081 cagacttatt ttataaggga gatactgtgc tgagttcttc cctttgagga aggtatgtca
1141 tatcctagac atagtctcaa ttttaaaaga agaggtagga taggagggat ggccccttat
1201 gaattattag cacaacaaga atccttaaga atacaagatt atttttctgc aataccacaa
1261 aaattgcaag cacagtggat ttattataaa gatcaaaaag ataagaaatg gaaaggacca
1321 atgagagtag aatactgggg acagggatca gtattattaa aggatgaaga gaagggatat
1381 tttcttataa tcgatactag tattatgccc agtacatgac cttatgggac tttcctactt
1441 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
1501 tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
1561 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact
1621 ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag
1681 ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata
1741 gaagattcta gagcccgggc gcgccggatc cagatcttaa ttaatttaaa tgaattcgcg
1801 gccgcgaagg atctgcgatc gctccggtgc ccgtcagtgg gcagagcgca catcgcccac
1861 agtccccgag aagttggggg gaggggtcgg caattgaacg ggtgcctaga gaaggtggcg
1921 cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg
1981 agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc
2041 cagaacacag ctgaagcttc gaggggctcg catctctcct tcacgcgccc gccgccctac
2101 ctgaggccgc catccacgcc ggttgagtcg cgttctgccg cctcccgcct gtggtgcctc
2161 ctgaactgcg tccgccgtct aggtaagttt aaagctcagg tcgagaccgg gcctttgtcc
2221 ggcgctccct tggagcctac ctagactcag ccggctctcc acgctttgcc tgaccctgct
2281 tgctcaactc tacgtctttg tttcgttttc tgttctgcgc cgttacagat ccaagctgtg
2341 accggcgcct acgctagatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg
2401 acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc
2461 acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca
2521 cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg
2581 tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca
2641 tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc
2701 cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc
2761 agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg
2821 gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg
2881 gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc
2941 gcaagcccgg tgcctgagtc gacaatcaac ctctggatta caaaatttgt gaaagattga
3001 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
3061 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
3121 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
3181 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
3241 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
3301 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat
3361 catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
3421 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
3481 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
3541 ccgcctcccc gcctgggtac cgatgacaga gttagaagat cgcttcagga agctatttgg
3601 cacgacttct acaacgggag acagcacagt agattctgaa gatgaacctc ctaaaaaaga
3661 aaaaagggtg gactgggatg agtattggaa ccctgaaatc gatagcttcc agtgctttgt
3721 gaaacttcga ggagtctctt tgttgaggac ttttgagttc tcccttgagg ctcccacaga
3781 tacaataaat atttgagatt gaaccctgtc gagtatctgt gtaatctttt ttacctgtga
3841 ggtctcggaa tccgggccga gaacttcgca gcgagctcat tgtaccgcga acttgtttat
3901 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
3961 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
4021 gctctagcta tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
4081 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
4141 tagtgaggag gcttttttgg aggcctagac ttttgcagag acggcccaaa ttcgtaatca
4201 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
4261 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
4321 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
4381 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
4441 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
4501 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
4561 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
4621 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
4681 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
4741 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
4801 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
4861 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
4921 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
4981 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
5041 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
5101 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
5161 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
5221 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
5281 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
5341 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
5401 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
5461 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
5521 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
5581 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
5641 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
5701 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
5761 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
5821 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
5881 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
5941 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
6001 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
6061 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
6121 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
6181 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
6241 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
6301 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
6361 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
6421 cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
6481 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
6541 ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga
6601 gtgcac
//