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产品名称：pTRIPZ
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简单介绍

pTRIPZ菌株发货前均经过严格的多重验证，如存在质量问题，请在收到产品的三个月内通知我司。pTRIPZ收到质粒后请短暂离心，加入20μl ddH2O溶解质粒，取2μl转化至对应感受态中，挑取单克隆重新提取质粒后使用。

产品描述

pTRIPZ

产品信息
基本信息
质粒简介
质粒图谱
质粒序列

产品信息

产品货号	产品名称	产品规格	优惠价
XY0703	pTRIPZ	20μl	¥请询价

使用说明

信裕质粒平台的各批次质粒菌株发货前均经过严格的多重验证，如存在质量问题，请在收到产品的三个月内通知我司。收到质粒后请短暂离心，加入20μl ddH2O溶解质粒，取2μl转化至对应感受态中，挑取单克隆重新提取质粒后使用。如需获取其他详细信息请登录我司官网查询。

基本信息

启动子:	T7，Ubc
复制子:	pUC ori,f1 ori,SV40 ori
终止子:	SV40 poly(A) signal，bGH poly(A) signal
质粒分类:	病毒系列，慢病毒干扰载体
质粒大小:	13320bp
原核抗性:	Amp
筛选标记:	Puro
克隆菌株:	Stbl3
培养条件:	37℃，有氧 LB
表达宿主:	哺乳细胞
诱导方式:	无须诱导，瞬时表达
5'测序引物:	T7:TAATACGACTCACTATAGGG
3'测序引物:	根据序列设计引物

质粒简介

The Thermo Scientific Open Biosystems TRIPZ Lentiviral Inducible shRNAmir Library was developed in collaboration with Dr. Greg Hannon (CSHL) and Dr. Steve Elledge (Harvard). This library combines the design advantages of microRNA-adapted shRNA (shRNAmir) with the pTRIPZ lentiviral inducible vector to create a powerful RNAi trigger capable of producing RNAi in most cell types. The vector is engineered to be Tet-On and produces tightly regulated induction of shRNAmir expression in the presence of doxycycline.

质粒图谱

质粒序列

LOCUS Exported 13320 bp ds-DNA circular SYN 13-JUL-2016

DEFINITION Empty lentiviral vector for doxycycline-inducible expression of an

shRNAmir together with TurboRFP.

ACCESSION .

VERSION .

KEYWORDS pTRIPZ

SOURCE synthetic DNA construct

ORGANISM synthetic DNA construct

REFERENCE 1 (bases 1 to 13320)

AUTHORS Open Biosystems

TITLE Direct Submission

JOURNAL Exported Friday, September 9, 2016 from SnapGene Viewer 3.1.4

http://www.miaolingbio.com

COMMENT Excision of rtTA3 using Cre recombinase or BamHI yields a vector

that can be used for Tet-repressible expression.

FEATURES Location/Qualifiers

source 1..13320

/organism="synthetic DNA construct"

/lab_host="Mammalian Cells"

/mol_type="other DNA"

LTR 1..634

/note="5' LTR"

/note="5' long terminal repeat (LTR) from HIV-1"

misc_feature 681..806

/note="HIV-1 psi"

/note="packaging signal of human immunodeficiency virus

type 1"

misc_feature 1303..1536

/note="RRE"

/note="The Rev response element (RRE) of HIV-1 allows for

Rev-dependent mRNA export from the nucleus to the

cytoplasm."

misc_feature 2063..2180

/note="cPPT/CTS"

/note="central polypurine tract and central termination

sequence of HIV-1"

CDS complement(2245..2619)

/codon_start=1

/gene="Sh ble from Streptoalloteichus hindustanus"

/product="antibiotic-binding protein"

/note="BleoR"

/note="confers resistance to bleomycin, phleomycin, and

Zeocin(TM)"

/translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD

VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR

EFALRDPAGNCVHFVAEEQD"

promoter complement(2639..2686)

/note="EM7 promoter"

/note="synthetic bacterial promoter "

protein_bind 2722..2740

/gene="tetO"

/bound_moiety="tetracycline repressor TetR"

/note="tet operator"