LOCUS Exported 11085 bp ds-DNA circular SYN 09-SEP-2016
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS Untitled 3
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 11085)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Friday, September 9, 2016 from SnapGene Viewer 3.1.4
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..11085
/organism="synthetic DNA construct"
/mol_type="other DNA"
polyA_signal 168..302
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
CDS complement(727..747)
/codon_start=1
/product="nuclear localization signal of SV40 large T
antigen"
/note="SV40 NLS"
/translation="PKKKRKV"
intron 877..942
/note="small t intron"
/note="simian virus 40 (SV40) small t antigen intron"
promoter complement(2076..2405)
/note="SV40 promoter"
/note="SV40 enhancer and early promoter"
rep_origin 2090..2225
/note="SV40 ori"
/note="SV40 origin of replication"
rep_origin complement(2651..3239)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(3410..4270)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(4271..4375)
/gene="bla"
/note="AmpR promoter"
promoter 5163..5181
/note="SP6 promoter"
/note="promoter for bacteriophage SP6 RNA polymerase"
LTR 5206..5386
/note="5' LTR (truncated)"
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
protein_bind complement(5406..5439)
/bound_moiety="Cre recombinase"
/note="loxP"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
promoter complement(5474..5688)
/note="H1 promoter"
/note="human H1 RNA promoter"
protein_bind 5717..5987
/bound_moiety="tetracycline repressor TetR"
/note="tetracycline response element"
/note="contains seven copies of the tetracycline operator
tetO"
misc_feature 6155..6743
/note="WPRE"
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
CDS 6261..6272
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/note="Factor Xa site"
/translation="IEGR"
CDS complement(6816..7532)
/codon_start=1
/product="enhanced GFP"
/note="EGFP"
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
misc_feature 7622..7739
/note="cPPT/CTS"
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter complement(7787..8964)
/note="EF-1-alpha promoter"
/note="strong constitutive promoter for human elongation
factor EF-1-alpha"
intron 7796..8733
/note="EF-1-alpha intron A"
/note="intron upstream of the start codon of human
EF-1-alpha"
misc_feature 9553..9786
/note="RRE"
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
misc_feature 10279..10404
/note="HIV-1 Psi"
/note="packaging signal of human immunodeficiency virus
type 1"
LTR 10451..11084
/note="3' LTR"
/note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
1 ctactaagtt tgtagtacat atttaacaaa tacaatttct ttaaaatgaa aataattcag
61 aggaatcaca ggtttagagt aaatgaaacc actaggtaat tggcagtggt aatagggtat
121 ggggtgggaa gtttgggatg attttggtta gcttgagtta tccagttgat ccagacatga
181 taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta
241 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
301 ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt
361 tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga ttatgatctc tagtcaaggc
421 actatacatc aaatattcct tattaacccc tttacaaatt aaaaagctaa aggtacacaa
481 tttttgagca tagttattaa tagcagacac tctatgcctg tgtggagtaa gaaaaaacag
541 tatgttatga ttataactgt tatgcctact tataaaggtt acagaatatt tttccataat
601 tttcttgtat agcagtgcag ctttttcctt tgtggtgtaa atagcaaagc aagcaagagt
661 tctattacta aacacagcat gactcaaaaa acttagcaat tctgaaggaa agtccttggg
721 gtcttctacc tttctcttct tttttggagg agtagaatgt tgagagtcag cagtagcctc
781 atcatcacta gatggcattt cttctgagca aaacaggttt tcctcattaa aggcattcca
841 ccactgctcc cattcatcag ttccataggt tggaatctaa aatacacaaa caattagaat
901 cagtagttta acacattata cacttaaaaa ttttatattt accttagagc tttaaatctc
961 tgtaggtagt ttgtccaatt atgtcacacc acagaagtaa ggttccttca caaagatccg
1021 gggcccactc ataaatccag ttgccgccac ggtagccaat caccgtatcg tataaatcat
1081 cgtcggtacg ttcggcatcg ctcatcacaa tacgtgcctg gacgtcgagg atttcgcgtg
1141 ggtcaatgcc gcgccagatc cacatcagac ggttaatcat gcgataccag tgagggatgg
1201 ttttaccatc aagggccgac tgcacaggcg gttgtgcgcc gtgattaaag cggcggacta
1261 gcgtcgaggt ttcaggatgt ttaaagcggg gtttgaacag ggtttcgctc aggtttgcct
1321 gtgtcatgga tgcagcctcc agaatactta ctggaaacta ttgtaacccg cctgaagtta
1381 aaaagaacaa cgcccggcag tgccaggcgt tgaaaagatt agcgaccgga gattggcggg
1441 acgaatacga cgcccatatc ccacggctgt tcaatccagg tatcttgcgg gatatcaaca
1501 acatagtcat caaccagcgg acgaccagcc ggttttgcga agatggtgac aaagtgcgct
1561 tttggataca tttcacgaat cgcaaccgca gtaccaccgg tatccaccag gtcatcaata
1621 acgatgaagc cttcgccatc gccttctgcg cgtttcagca ctttaagctc gcgctggttg
1681 tcgtgatcgt agctggaaat acaaacggta tcgacatgac gaatacccag ttcacgcgcc
1741 agtaacgcac ccggtacaga ccgccacggc ttacggcaat aatgcctttc cattgttcag
1801 aaggcatcag tcggcttgcg agtttacgtg catggatctg caacatgtcc caggtgacga
1861 tgtatttttc gctcatgtga agtgtcccag cctgtttatc tacggcttaa aaagtgttcg
1921 aggggaaaat aggttgcgcg agattataga gatctggcgc actaaaaacc agtatttcac
1981 atgagtccgc gtctttttac gcactgcctc tccctgacgc gggataaagt ggtattctca
2041 aacatatctc gcaagcctgt cttgtgtcca agctttttgc aaaagcctag gcctccaaaa
2101 aagcctcctc actacttctg gaatagctca gaggccgagg cggcctcggc ctctgcataa
2161 ataaaaaaaa ttagtcagcc atggggcgga gaatgggcgg aactgggcgg agttaggggc
2221 gggatgggcg gagttagggg cgggactatg gttgctgact aattgagatg catgctttgc
2281 atacttctgc ctgctgggga gcctggggac tttccacacc tggttgctga ctaattgaga
2341 tgcatgcttt gcatacttct gcctgctggg gagcctgggg actttccaca ccctaactga
2401 cacacattcc acagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
2461 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
2521 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
2581 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
2641 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
2701 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
2761 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
2821 cttcgggaag cgtggcgctt tctcaatgct cacgctgtag gtatctcagt tcggtgtagg
2881 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
2941 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
3001 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
3061 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
3121 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
3181 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
3241 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
3301 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
3361 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
3421 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
3481 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
3541 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
3601 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
3661 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
3721 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
3781 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
3841 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
3901 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
3961 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
4021 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
4081 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
4141 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
4201 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
4261 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
4321 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
4381 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
4441 aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc
4501 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca
4561 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg
4621 cggcatcaga gcagattgta ctgagagtgc accattcgac gctctccctt atgcgactcc
4681 tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat
4741 ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc
4801 acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg
4861 tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt
4921 ccggcgtaga ggatctggct agcgatgacc ctgctgattg gttcgctgac catttccggg
4981 tgcgggacgg cgttaccaga aactcagaag gttcgtccaa ccaaaccgac tctgacggca
5041 gtttacgaga gagatgatag ggtctgcttc agtaagccag atgctacaca attaggcttg
5101 tacatattgt cgttagaacg cggctacaat taatacataa ccttatgtat catacacata
5161 cgatttaggt gacactatag aatacacgga attaattcta gatgctgcta gagattttcc
5221 acactgacta aaagggtctg agggatctct agttaccaga gtcacacaac agacgggcac
5281 acactacttg aagcactcaa ggcaagcttt attgaggctt aagcagtggg ttccctagtt
5341 agccagagag ctcccaggct cagatctggt ctaaccagag agacccagta caagcaaaaa
5401 gcagcataac ttcgtataat gtatgctata cgaagttatt cccatgcgac ggtatcgata
5461 agcttggaga cgcgtggtct catacagaac ttataagatt cccaaatcca aagacatttc
5521 acgtttatgg tgatttccca gaacacatag cgacatgcaa atattgcagg gcgccactcc
5581 cctgtccctc acagccatct tcctgccagg gcgcacgcgc gctgggtgtt cccgcctagt
5641 gacactgggc ccgcgattcc ttggagcggg ttgatgacgt cagcgttcga attcctgcag
5701 ccccgacttt cacttttctc tatcactgat agggagtggt aaactcgact ttcacttttc
5761 tctatcactg atagggagtg gtaaactcga ctttcacttt tctctatcac tgatagggag
5821 tggtaaactc gactttcact tttctctatc actgataggg agtggtaaac tcgactttca
5881 cttttctcta tcactgatag ggagtggtaa actcgacttt cacttttctc tatcactgat
5941 agggagtggt aaactcgact ttcacttttc tctatcactg atagggagtg gtaaactcga
6001 gggggatcca ctagcatggt gaattgatcc catcttgtct tcgttgggag tgaattagcc
6061 cttccagtcc ccccttttct tttaaaaagt ggctaagatc tacagctgcc ttgtaagtca
6121 ttggtcttaa aggtacctgg ccatacgtac cgatgcgggg aggcggccca aagggagatc
6181 cgactcgtct gagggcgaag gcgaagacgc ggaagaggcc gcagagccgg cagcaggccg
6241 cgggaaggaa ggtccgctgg attgagggcc gaagggacgt agcagaagga cgtcccgcgc
6301 agaatccagg tggcaacaca ggcgagcagc catggaaagg acgtcagctt ccccgacaac
6361 accacggaat tgtcagtgcc caacagccga gcccctgtcc agcagcgggc aaggcaggcg
6421 gcgatgagtt ccgccgtggc aatagggagg gggaaagcga aagtcccgga aaggagctga
6481 caggtggtgg caatgcccca accagtgggg gttgcgtcag caaacacagt gcacaccacg
6541 ccacgttgcc tgacaacggg ccacaactcc tcataaagag acagcaacca ggatttatac
6601 aaggaggaga aaatgaaagc catacgggaa gcaatagcat gatacaaagg cattaaagca
6661 gcgtatccac atagcgtaaa aggagcaaca tagttaagaa taccagtcaa tctttcacaa
6721 attttgtaat ccagaggttg attatcatat gactagtccc cgaagttgag ctcgactagc
6781 tagctagcta ctagctatcg agatctgagt ccggacttgt acagctcgtc catgccgaga
6841 gtgatcccgg cggcggtcac gaactccagc aggaccatgt gatcgcgctt ctcgttgggg
6901 tctttgctca gggcggactg ggtgctcagg tagtggttgt cgggcagcag cacggggccg
6961 tcgccgatgg gggtgttctg ctggtagtgg tcggcgagct gcacgctgcc gtcctcgatg
7021 ttgtggcgga tcttgaagtt caccttgatg ccgttcttct gcttgtcggc catgatatag
7081 acgttgtggc tgttgtagtt gtactccagc ttgtgcccca ggatgttgcc gtcctccttg
7141 aagtcgatgc ccttcagctc gatgcggttc accagggtgt cgccctcgaa cttcacctcg
7201 gcgcgggtct tgtagttgcc gtcgtccttg aagaagatgg tgcgctcctg gacgtagcct
7261 tcgggcatgg cggacttgaa gaagtcgtgc tgcttcatgt ggtcggggta gcggctgaag
7321 cactgcacgc cgtaggtcag ggtggtcacg agggtgggcc agggcacggg cagcttgccg
7381 gtggtgcaga tgaacttcag ggtcagcttg ccgtaggtgg catcgccctc gccctcgccg
7441 gacacgctga acttgtggcc gtttacgtcg ccgtccagct cgaccaggat gggcaccacc
7501 ccggtgaaca gctcctcgcc cttgctcacc atggtggcga ccggtagcgc taggacgcgc
7561 gcgtaagctt aggccttcga agatcccgta gtttaaacct cgaggctagt ctcgtgatcg
7621 gaaaattttg aatttttgta atttgttttt gtaattcttt agtttgtatg tctgttgcta
7681 ttatgtctac tattctttcc cctgcactgt accccccaat cccccctttt cttttaaaag
7741 ttaaccgata ccgtcgagat taattaaatt taaatgtcga aattcctcac gacacctgaa
7801 atggaagaaa aaaactttga accactgtct gaggcttgag aatgaaccaa gatccaaact
7861 caaaaagggc aaattccaag gagaattaca tcaagtgcca agctggccta acttcagtct
7921 ccacccactc agtgtgggga aactccatcg cataaaaccc ctccccccaa cctaaagacg
7981 acgtactcca aaagctcgag aactaatcga ggtgcctgga cggcgcccgg tactccgtgg
8041 agtcacatga agcgacggct gaggacggaa aggccctttt cctttgtgtg ggtgactcac
8101 ccgcccgctc tcccgagcgc cgcgtcctcc attttgagct ccctgcagca gggccgggaa
8161 gcggccatct ttccgctcac gcaactggtg ccgaccgggc cagccttgcc gcccagggcg
8221 gggcgataca cggcggcgcg aggccaggca ccagagcagg ccggccagct tgagactacc
8281 cccgtccgat tctcggtggc cgcgctcgca ggccccgcct cgccgaacat gtgcgctggg
8341 acgcacgggc cccgtcgccg cccgcggccc caaaaaccga aataccagtg tgcagatctt
8401 ggcccgcatt tacaagacta tcttgccaga aaaaaagcgt cgcagcaggt catcaaaaat
8461 tttaaatggc tagagactta tcgaaagcag cgagacaggc gcgaaggtgc caccagattc
8521 gcacgcggcg gccccagcgc ccaggccagg cctcaactca agcacgaggc gaaggggctc
8581 cttaagcgca aggcctcgaa ctctcccacc cacttccaac ccgaagctcg ggatcaagaa
8641 tcacgtactg cagccagtgg aagtaattca aggcacgcaa gggccataac ccgtaaagag
8701 gccaggcccg cgggaaccac acacggcact tacctgtgtt ctggcggcaa acccgttgcg
8761 aaaaagaacg ttcacggcga ctactgcact tatatacggt tctcccccac cctcgggaaa
8821 aaggcggagc cagtacacga catcactttc ccagtttacc ccgcgccacc ttctctaggc
8881 accggttcaa ttgccgaccc ctccccccaa cttctcgggg actgtgggcg atgtgcgctc
8941 tgcccactga cgggcaccgg agccaattcc cactcctttc aagacctaga aggtccatta
9001 gctgcaaaga ttcctctctg tttaaaactt tatccatctt tgcaaagctt atcgtcgaca
9061 tcgcgatacc gtcgagatcc gttcactaat cgaatggatc tgtctctgtc tctctctcca
9121 ccttcttctt ctattccttc gggcctgtcg ggtcccctcg gggttgggag gtgggtctga
9181 aacgataatg gtgaatatcc ctgcctaact ctattcacta tagaaagtac agcaaaaact
9241 attcttaaac ctaccaagcc tcctactatc attatgaata attttatata ccacagccaa
9301 tttgttatgt taaaccaatt ccacaaactt gcccatttat ctaattccaa taattcttgt
9361 tcattctttt cttgctggtt ttgcgattct tcaattaagg agtgtattaa gcttgtgtaa
9421 ttgttaattt ctctgtccca ctccatccag gtcgtgtgat tccaaatctg ttccagagat
9481 ttattactcc aactagcatt ccaaggcaca gcagtggtgc aaatgagttt tccagagcaa
9541 ccccaaatcc ccaggagctg ttgatccttt aggtatcttt ccacagccag gattcttgcc
9601 tggagctgct tgatgcccca gactgtgagt tgcaacagat gctgttgcgc ctcaatagcc
9661 ctcagcaaat tgttctgctg ctgcactata ccagacaata attgtctggc ctgtaccgtc
9721 agcgtcattg acgctgcgcc catagtgctt cctgctgctc ccaagaaccc aaggaacaaa
9781 gctcctattc ccactgctct tttttctctc tgcaccactc ttctctttgc cttggtgggt
9841 gctactccta atggttcaat ttttactact ttatatttat ataattcact tctccaattg
9901 tccctcatat ctcctcctcc aggtctgaag atcagcggcc gcttgctgtg cggtggtctt
9961 acttttgttt tgctcttcct ctatcttgtc taaagcttcc ttggtgtctt ttatctctat
10021 cctttgatgc acacaataga gggttgctac tgtattatat aatgatctaa gttcttctga
10081 tcctgtctga agggatggtt gtagctgtcc cagtatttgt ctacagcctt ctgatgtttc
10141 taacaggcca ggattaactg cgaatcgttc tagctccctg cttgcccata ctatatgttt
10201 taatttatat tttttctttc cccctggcct taaccgaatt ttttcccatc gcgatctaat
10261 tctcccccgc ttaatactga cgctctcgca cccatctctc tccttctagc ctccgctagt
10321 caaaattttt ggcgtactca ccagtcgccg cccctcgcct cttgccgtgc gcgcttcagc
10381 aagccgagtc ctgcgtcgag agagctcctc tggtttccct ttcgctttca agtccctgtt
10441 cgggcgccac tgctagagat tttccacact gactaaaagg gtctgaggga tctctagtta
10501 ccagagtcac acaacagacg ggcacacact acttgaagca ctcaaggcaa gctttattga
10561 ggcttaagca gtgggttccc tagttagcca gagagctccc aggctcagat ctggtctaac
10621 cagagagacc cagtacaggc aaaaagcagc tgcttatatg caggatctga gggctcgcca
10681 ctccccagtc ccgcccaggc cacgcctccc tggaaagtcc ccagcggaaa gtcccttgta
10741 gcaagctcga tatcagcagt tcttgaagta ctccggatgc agctctcggg ccacgtgatg
10801 aaatgctagg cggctgtcaa acctccactc taacacttct ctctccgggt catccatccc
10861 atgcaggctc acagggtgta acaagctggt gttctctcct ttattggcct cttctacctt
10921 atctggctca actggtacta gcttgtagca ccatccaaag gtcagtggat atctgacccc
10981 tggccctggt gtgtagttct gctaatcagg gaagtagcct tgtgtgtggt agatccacag
11041 atcaaggata tcttgtcttc tttgggagtg aattagccct tccaa
//