LOCUS Exported 14174 bp ds-DNA circular SYN 27-NOV-2017
DEFINITION synthetic circular DNA
FEATURES Location/Qualifiers
source 1..14174
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 315..991
/label=CaMV 35S promoter (enhanced)
/note="cauliflower mosaic virus 35S promoter with a
duplicated enhancer region"
CDS 1058..2083
/codon_start=1
/gene="aph(4)-Ia"
/product="aminoglycoside phosphotransferase from E. coli"
/label=HygR
/note="confers resistance to hygromycin"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
K"
polyA_signal 2124..2298
/label=CaMV poly(A) signal
/note="cauliflower mosaic virus polyadenylation signal"
misc_feature 2376..2400
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA"
CDS 4024..4818
/codon_start=1
/gene="aphA-3"
/product="aminoglycoside phosphotransferase"
/label=KanR
/note="confers resistance to kanamycin"
/translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
rep_origin 4905..5493
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
misc_feature 5679..5819
/label=bom
/note="basis of mobility region from pBR322"
rep_origin 6163..6357
/label=pVS1 oriV
/note="origin of replication for the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
CDS complement(6423..7496)
/codon_start=1
/product="replication protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/label=pVS1 RepA
/translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
LVMRYRNLIEGEASAGS"
CDS complement(7925..8554)
/codon_start=1
/product="stability protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/label=pVS1 StaA
/translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
misc_feature 9849..9873
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
promoter 10108..12105
/label=Ubi promoter
/note="maize polyubiquitin gene promoter"
protein_bind 12148..12272
/gene="mutant version of attR"
/label=attR1
/bound_moiety="LR Clonase(TM)"
/note="recombination site for the Gateway(R) LR reaction"
promoter 12297..12327
/label=lac UV5 promoter
/note="E. coli lac promoter with an ""up"" mutation"
CDS 12381..13061
/codon_start=1
/product="chloramphenicol acetyltransferase"
/label=CmR
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
DPAY"
CDS 13381..13686
/codon_start=1
/gene="ccdB"
/product="CcdB, a bacterial toxin that poisons DNA gyrase"
/label=ccdB
/note="Plasmids containing the ccdB gene cannot be
propagated in standard E. coli strains."
/translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
protein_bind complement(13727..13851)
/gene="mutant version of attR"
/label=attR2
/bound_moiety="LR Clonase(TM)"
/note="recombination site for the Gateway(R) LR reaction"
terminator 13915..14167
/label=NOS terminator
/note="nopaline synthase terminator and poly(A) signal"
ORIGIN
1 cgtaatcatg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
61 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
121 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
181 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attggctaga gcagcttgcc
241 aacatggtgg agcacgacac tctcgtctac tccaagaata tcaaagatac agtctcagaa
301 gaccaaaggg ctattgagac ttttcaacaa agggtaatat cgggaaacct cctcggattc
361 cattgcccag ctatctgtca cttcatcaaa aggacagtag aaaaggaagg tggcacctac
421 aaatgccatc attgcgataa aggaaaggct atcgttcaag atgcctctgc cgacagtggt
481 cccaaagatg gacccccacc cacgaggagc atcgtggaaa aagaagacgt tccaaccacg
541 tcttcaaagc aagtggattg atgtgaacat ggtggagcac gacactctcg tctactccaa
601 gaatatcaaa gatacagtct cagaagacca aagggctatt gagacttttc aacaaagggt
661 aatatcggga aacctcctcg gattccattg cccagctatc tgtcacttca tcaaaaggac
721 agtagaaaag gaaggtggca cctacaaatg ccatcattgc gataaaggaa aggctatcgt
781 tcaagatgcc tctgccgaca gtggtcccaa agatggaccc ccacccacga ggagcatcgt
841 ggaaaaagaa gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg atatctccac
901 tgacgtaagg gatgacgcac aatcccacta tccttcgcaa gacccttcct ctatataagg
961 aagttcattt catttggaga ggacacgctg aaatcaccag tctctctcta caaatctatc
1021 tctctcgagc tttcgcagat ccggggggca atgagatatg aaaaagcctg aactcaccgc
1081 gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc gtctccgacc tgatgcagct
1141 ctcggagggc gaagaatctc gtgctttcag cttcgatgta ggagggcgtg gatatgtcct
1201 gcgggtaaat agctgcgccg atggtttcta caaagatcgt tatgtttatc ggcactttgc
1261 atcggccgcg ctcccgattc cggaagtgct tgacattggg gagtttagcg agagcctgac
1321 ctattgcatc tcccgccgtt cacagggtgt cacgttgcaa gacctgcctg aaaccgaact
1381 gcccgctgtt ctacaaccgg tcgcggaggc tatggatgcg atcgctgcgg ccgatcttag
1441 ccagacgagc gggttcggcc cattcggacc gcaaggaatc ggtcaataca ctacatggcg
1501 tgatttcata tgcgcgattg ctgatcccca tgtgtatcac tggcaaactg tgatggacga
1561 caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg atgctttggg ccgaggactg
1621 ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc aacaatgtcc tgacggacaa
1681 tggccgcata acagcggtca ttgactggag cgaggcgatg ttcggggatt cccaatacga
1741 ggtcgccaac atcttcttct ggaggccgtg gttggcttgt atggagcagc agacgcgcta
1801 cttcgagcgg aggcatccgg agcttgcagg atcgccacga ctccgggcgt atatgctccg
1861 cattggtctt gaccaactct atcagagctt ggttgacggc aatttcgatg atgcagcttg
1921 ggcgcagggt cgatgcgacg caatcgtccg atccggagcc gggactgtcg ggcgtacaca
1981 aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt gtagaagtac tcgccgatag
2041 tggaaaccga cgccccagca ctcgtccgag ggcaaagaaa tagagtagat gccgaccggg
2101 atctgtcgat cgacaagctc gagtttctcc ataataatgt gtgagtagtt cccagataag
2161 ggaattaggg ttcctatagg gtttcgctca tgtgttgagc atataagaaa cccttagtat
2221 gtatttgtat ttgtaaaata cttctatcaa taaaatttct aattcctaaa accaaaatcc
2281 agtactaaaa tccagatccc ccgaattaat tcggcgttaa ttcagtacat taaaaacgtc
2341 cgcaatgtgt tattaagttg tctaagcgtc aatttgttta caccacaata tatcctgcca
2401 ccagccagcc aacagctccc cgaccggcag ctcggcacaa aatcaccact cgatacaggc
2461 agcccatcag tccgggacgg cgtcagcggg agagccgttg taaggcggca gactttgctc
2521 atgttaccga tgctattcgg aagaacggca actaagctgc cgggtttgaa acacggatga
2581 tctcgcggag ggtagcatgt tgattgtaac gatgacagag cgttgctgcc tgtgatcacc
2641 gcggtttcaa aatcggctcc gtcgatacta tgttatacgc caactttgaa aacaactttg
2701 aaaaagctgt tttctggtat ttaaggtttt agaatgcaag gaacagtgaa ttggagttcg
2761 tcttgttata attagggaag gtgcgaacaa gtccctgata tgagatcatg tttgtcatct
2821 ggagccatag aacagggttc atcatgagtc atcaacttac cttcgccgac agtgaattca
2881 gcagtaagcg ccgtcagacc agaaaagaga ttttcttgtc ccgcatggag cagattctgc
2941 catggcaaaa catggtggaa gtcatcgagc cgttttaccc caaggctggt aatggccggc
3001 gaccttatcc gctggaaacc atgctacgca ttcactgcat gcagcattgg tacaacctga
3061 gcgatggcgc gatggaagat gctctgtacg aaatcgcctc catgcgtctg tttgcccggt
3121 tatccctgga tagcgccttg ccggaccgca ccaccatcat gaatttccgc cacctgctgg
3181 agcagcatca actggcccgc caattgttca agaccatcaa tcgctggctg gccgaagcag
3241 gcgtcatgat gactcaaggc accttggtcg atgccaccat cattgaggca cccagctcga
3301 ccaagaacaa agagcagcaa cgcgatccgg agatgcatca gaccaagaaa ggcaatcagt
3361 ggcactttgg catgaaggcc cacattggtg tcgatgccaa gagtggcctg acccacagcc
3421 tggtcaccac cgcggccaac gagcatgacc tcaatcagct gggtaatctg ctgcatggag
3481 aggagcaatt tgtctcagcc gatgccggct accaaggggc gccacagcgc gaggagctgg
3541 ccgaggtgga tgtggactgg ctgatcgccg agcgccccgg caaggtaaga accttgaaac
3601 agcatccacg caagaacaaa acggccatca acatcgaata catgaaagcc agcatccggg
3661 ccagggtgga gcacccattt cgcatcatca agcgacagtt cggcttcgtg aaagccagat
3721 acaaggggtt gctgaaaaac gataaccaac tggcgatgtt attcacgctg gccaacctgt
3781 ttcgggcgga ccaaatgata cgtcagtggg agagatctca ctaaaaactg gggataacgc
3841 cttaaatggc gaagaaacgg tctaaatagg ctgattcaag gcatttacgg gagaaaaaat
3901 cggctcaaac atgaagaaat gaaatgactg agtcagccga gaagaatttc cccgcttatt
3961 cgcaccttcc ttagcttctt ggggtatctt taaatactgt agaaaagagg aaggaaataa
4021 taaatggcta aaatgagaat atcaccggaa ttgaaaaaac tgatcgaaaa ataccgctgc
4081 gtaaaagata cggaaggaat gtctcctgct aaggtatata agctggtggg agaaaatgaa
4141 aacctatatt taaaaatgac ggacagccgg tataaaggga ccacctatga tgtggaacgg
4201 gaaaaggaca tgatgctatg gctggaagga aagctgcctg ttccaaaggt cctgcacttt
4261 gaacggcatg atggctggag caatctgctc atgagtgagg ccgatggcgt cctttgctcg
4321 gaagagtatg aagatgaaca aagccctgaa aagattatcg agctgtatgc ggagtgcatc
4381 aggctctttc actccatcga catatcggat tgtccctata cgaatagctt agacagccgc
4441 ttagccgaat tggattactt actgaataac gatctggccg atgtggattg cgaaaactgg
4501 gaagaagaca ctccatttaa agatccgcgc gagctgtatg attttttaaa gacggaaaag
4561 cccgaagagg aacttgtctt ttcccacggc gacctgggag acagcaacat ctttgtgaaa
4621 gatggcaaag taagtggctt tattgatctt gggagaagcg gcagggcgga caagtggtat
4681 gacattgcct tctgcgtccg gtcgatcagg gaggatatcg gggaagaaca gtatgtcgag
4741 ctattttttg acttactggg gatcaagcct gattgggaga aaataaaata ttatatttta
4801 ctggatgaat tgttttagta cctagaatgc atgaccaaaa tcccttaacg tgagttttcg
4861 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
4921 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
4981 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
5041 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
5101 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
5161 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
5221 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
5281 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
5341 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
5401 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
5461 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
5521 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
5581 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
5641 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt
5701 acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat
5761 gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc
5821 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
5881 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
5941 caccgaaacg cgcgaggcag ggtgccttga tgtgggcgcc ggcggtcgag tggcgacggc
6001 gcggcttgtc cgcgccctgg tagattgcct ggccgtaggc cagccatttt tgagcggcca
6061 gcggccgcga taggccgacg cgaagcggcg gggcgtaggg agcgcagcga ccgaagggta
6121 ggcgcttttt gcagctcttc ggctgtgcgc tggccagaca gttatgcaca ggccaggcgg
6181 gttttaagag ttttaataag ttttaaagag ttttaggcgg aaaaatcgcc ttttttctct
6241 tttatatcag tcacttacat gtgtgaccgg ttcccaatgt acggctttgg gttcccaatg
6301 tacgggttcc ggttcccaat gtacggcttt gggttcccaa tgtacgtgct atccacagga
6361 aagagacctt ttcgaccttt ttcccctgct agggcaattt gccctagcat ctgctccgta
6421 cattaggaac cggcggatgc ttcgccctcg atcaggttgc ggtagcgcat gactaggatc
6481 gggccagcct gccccgcctc ctccttcaaa tcgtactccg gcaggtcatt tgacccgatc
6541 agcttgcgca cggtgaaaca gaacttcttg aactctccgg cgctgccact gcgttcgtag
6601 atcgtcttga acaaccatct ggcttctgcc ttgcctgcgg cgcggcgtgc caggcggtag
6661 agaaaacggc cgatgccggg atcgatcaaa aagtaatcgg ggtgaaccgt cagcacgtcc
6721 gggttcttgc cttctgtgat ctcgcggtac atccaatcag ctagctcgat ctcgatgtac
6781 tccggccgcc cggtttcgct ctttacgatc ttgtagcggc taatcaaggc ttcaccctcg
6841 gataccgtca ccaggcggcc gttcttggcc ttcttcgtac gctgcatggc aacgtgcgtg
6901 gtgtttaacc gaatgcaggt ttctaccagg tcgtctttct gctttccgcc atcggctcgc
6961 cggcagaact tgagtacgtc cgcaacgtgt ggacggaaca cgcggccggg cttgtctccc
7021 ttcccttccc ggtatcggtt catggattcg gttagatggg aaaccgccat cagtaccagg
7081 tcgtaatccc acacactggc catgccggcc ggccctgcgg aaacctctac gtgcccgtct
7141 ggaagctcgt agcggatcac ctcgccagct cgtcggtcac gcttcgacag acggaaaacg
7201 gccacgtcca tgatgctgcg actatcgcgg gtgcccacgt catagagcat cggaacgaaa
7261 aaatctggtt gctcgtcgcc cttgggcggc ttcctaatcg acggcgcacc ggctgccggc
7321 ggttgccggg attctttgcg gattcgatca gcggccgctt gccacgattc accggggcgt
7381 gcttctgcct cgatgcgttg ccgctgggcg gcctgcgcgg ccttcaactt ctccaccagg
7441 tcatcaccca gcgccgcgcc gatttgtacc gggccggatg gtttgcgacc gctcacgccg
7501 attcctcggg cttgggggtt ccagtgccat tgcagggccg gcaggcaacc cagccgctta
7561 cgcctggcca accgcccgtt cctccacaca tggggcattc cacggcgtcg gtgcctggtt
7621 gttcttgatt ttccatgccg cctcctttag ccgctaaaat tcatctactc atttattcat
7681 ttgctcattt actctggtag ctgcgcgatg tattcagata gcagctcggt aatggtcttg
7741 ccttggcgta ccgcgtacat cttcagcttg gtgtgatcct ccgccggcaa ctgaaagttg
7801 acccgcttca tggctggcgt gtctgccagg ctggccaacg ttgcagcctt gctgctgcgt
7861 gcgctcggac ggccggcact tagcgtgttt gtgcttttgc tcattttctc tttacctcat
7921 taactcaaat gagttttgat ttaatttcag cggccagcgc ctggacctcg cgggcagcgt
7981 cgccctcggg ttctgattca agaacggttg tgccggcggc ggcagtgcct gggtagctca
8041 cgcgctgcgt gatacgggac tcaagaatgg gcagctcgta cccggccagc gcctcggcaa
8101 cctcaccgcc gatgcgcgtg cctttgatcg cccgcgacac gacaaaggcc gcttgtagcc
8161 ttccatccgt gacctcaatg cgctgcttaa ccagctccac caggtcggcg gtggcccata
8221 tgtcgtaagg gcttggctgc accggaatca gcacgaagtc ggctgccttg atcgcggaca
8281 cagccaagtc cgccgcctgg ggcgctccgt cgatcactac gaagtcgcgc cggccgatgg
8341 ccttcacgtc gcggtcaatc gtcgggcggt cgatgccgac aacggttagc ggttgatctt
8401 cccgcacggc cgcccaatcg cgggcactgc cctggggatc ggaatcgact aacagaacat
8461 cggccccggc gagttgcagg gcgcgggcta gatgggttgc gatggtcgtc ttgcctgacc
8521 cgcctttctg gttaagtaca gcgataacct tcatgcgttc cccttgcgta tttgtttatt
8581 tactcatcgc atcatatacg cagcgaccgc atgacgcaag ctgttttact caaatacaca
8641 tcaccttttt agacggcggc gctcggtttc ttcagcggcc aagctggccg gccaggccgc
8701 cagcttggca tcagacaaac cggccaggat ttcatgcagc cgcacggttg agacgtgcgc
8761 gggcggctcg aacacgtacc cggccgcgat catctccgcc tcgatctctt cggtaatgaa
8821 aaacggttcg tcctggccgt cctggtgcgg tttcatgctt gttcctcttg gcgttcattc
8881 tcggcggccg ccagggcgtc ggcctcggtc aatgcgtcct aggcaccgcg ccgcctggcc
8941 tcggtgggcg tcacttcctc gctgcgctca agtgcgcggt acagggtcga gcgatgcacg
9001 ccaagcagtg cagccgcctc tttcacggtg cggccttcct ggtcgatcag ctcgcgggcg
9061 tgcgcgatct gtgccggggt gagggtaggg cgggggccaa acttcacgcc tcgggccttg
9121 gcggcctcgc gcccgctccg ggtgcggtcg atgattaggg aacgctcgaa ctcggcaatg
9181 ccggcgaaca cggtcaacac catgcggccg gccggcgtgg tggtgtcggc ccacggctct
9241 gccaggctac gcaggcccgc gccggcctcc tggatgcgct cggcaatgtc cagtaggtcg
9301 cgggtgctgc gggccaggcg gtctagcctg gtcactgtca caacgtcgcc agggcgtagg
9361 tggtcaagca tcctggccag ctccgggcgg tcgcgcctgg tgccggtgat cttctcggaa
9421 aacagcttgg tgcagccggc cgcgtgcagt tcggcccgtt ggttggtcaa gtcctggtcg
9481 tcggtgctga cgcgggcata gcccagcagg ccagcggcgg cgctcttgtt catggcgtaa
9541 tgtctccggt tctagtcgca agtattctac tttatgcgac taaaacacgc gacaagaaaa
9601 cgccaggaaa agggcagggc ggcagcctgt cgcgtaactt aggacttgtg cgacatgtcg
9661 ttttcagaag acggctgcac tgaacgtcag aagccgactg cactatagca gcggaggggt
9721 tggatcaaag tactttgatc ccgaggggaa ccctgtggtt ggcatgcaca tacaaatgga
9781 cgaacggata aaccttttca cgccctttta aatatccgat tattctaata aacgctcttt
9841 tctcttaggt ttacccgcca atatatcctg tcaaacactg atagtttaaa ctgaaggcgg
9901 gaaacgacaa tctgatccaa gctcaagctg ctctagcatt cgccattcag gctgcgcaac
9961 tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga
10021 tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa
10081 acgacggcca gtgccaagct tgcatgcctg cagtgcagcg tgacccggtc gtgcccctct
10141 ctagagataa tgagcattgc atgtctaagt tataaaaaat taccacatat tttttttgtc
10201 acacttgttt gaagtgcagt ttatctatct ttatacatat atttaaactt tactctacga
10261 ataatataat ctatagtact acaataatat cagtgtttta gagaatcata taaatgaaca
10321 gttagacatg gtctaaagga caattgagta ttttgacaac aggactctac agttttatct
10381 ttttagtgtg catgtgttct cctttttttt tgcaaatagc ttcacctata taatacttca
10441 tccattttat tagtacatcc atttagggtt tagggttaat ggtttttata gactaatttt
10501 tttagtacat ctattttatt ctattttagc ctctaaatta agaaaactaa aactctattt
10561 tagttttttt atttaataat ttagatataa aatagaataa aataaagtga ctaaaaatta
10621 aacaaatacc ctttaagaaa ttaaaaaaac taaggaaaca tttttcttgt ttcgagtaga
10681 taatgccagc ctgttaaacg ccgtcgacga gtctaacgga caccaaccag cgaaccagca
10741 gcgtcgcgtc gggccaagcg aagcagacgg cacggcatct ctgtcgctgc ctctggaccc
10801 ctctcgagag ttccgctcca ccgttggact tgctccgctg tcggcatcca gaaattgcgt
10861 ggcggagcgg cagacgtgag ccggcacggc aggcggcctc ctcctcctct cacggcaccg
10921 gcagctacgg gggattcctt tcccaccgct ccttcgcttt cccttcctcg cccgccgtaa
10981 taaatagaca ccccctccac accctctttc cccaacctcg tgttgttcgg agcgcacaca
11041 cacacaacca gatctccccc aaatccaccc gtcggcacct ccgcttcaag gtacgccgct
11101 cgtcctcccc cccccccccc tctctacctt ctctagatcg gcgttccggt ccatggttag
11161 ggcccggtag ttctacttct gttcatgttt gtgttagatc cgtgtttgtg ttagatccgt
11221 gctgctagcg ttcgtacacg gatgcgacct gtacgtcaga cacgttctga ttgctaactt
11281 gccagtgttt ctctttgggg aatcctggga tggctctagc cgttccgcag acgggatcga
11341 tttcatgatt ttttttgttt cgttgcatag ggtttggttt gcccttttcc tttatttcaa
11401 tatatgccgt gcacttgttt gtcgggtcat cttttcatgc ttttttttgt cttggttgtg
11461 atgatgtggt ctggttgggc ggtcgttcta gatcggagta gaattaattc tgtttcaaac
11521 tacctggtgg atttattaat tttggatctg tatgtgtgtg ccatacatat tcatagttac
11581 gaattgaaga tgatggatgg aaatatcgat ctaggatagg tatacatgtt gatgcgggtt
11641 ttactgatgc atatacagag atgctttttg ttcgcttggt tgtgatgatg tggtgtggtt
11701 gggcggtcgt tcattcgttc tagatcggag tagaatactg tttcaaacta cctggtgtat
11761 ttattaattt tggaactgta tgtgtgtgtc atacatcttc atagttacga gtttaagatg
11821 gatggaaata tcgatctagg ataggtatac atgttgatgt gggttttact gatgcatata
11881 catgatggca tatgcagcat ctattcatat gctctaacct tgagtaccta tctattataa
11941 taaacaagta tgttttataa ttattttgat cttgatatac ttggatgatg gcatatgcag
12001 cagctatatg tggatttttt tagccctgcc ttcatacgct atttatttgc ttggtactgt
12061 ttcttttgtc gatgctcacc ctgttgtttg gtgttacttc tgcaggtcga ctctagagga
12121 tcccctcgag gcgcgccaag ctatcaaaca agtttgtaca aaaaagctga acgagaaacg
12181 taaaatgata taaatatcaa tatattaaat tagattttgc ataaaaaaca gactacataa
12241 tactgtaaaa cacaacatat ccagtcacta tggcggccgc attaggcacc ccaggcttta
12301 cactttatgc ttccggctcg tataatgtgt ggattttgag ttaggatccg tcgagatttt
12361 caggagctaa ggaagctaaa atggagaaaa aaatcactgg atataccacc gttgatatat
12421 cccaatggca tcgtaaagaa cattttgagg catttcagtc agttgctcaa tgtacctata
12481 accagaccgt tcagctggat attacggcct ttttaaagac cgtaaagaaa aataagcaca
12541 agttttatcc ggcctttatt cacattcttg cccgcctgat gaatgctcat ccggaattcc
12601 gtatggcaat gaaagacggt gagctggtga tatgggatag tgttcaccct tgttacaccg
12661 ttttccatga gcaaactgaa acgttttcat cgctctggag tgaataccac gacgatttcc
12721 ggcagtttct acacatatat tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt
12781 tccctaaagg gtttattgag aatatgtttt tcgtctcagc caatccctgg gtgagtttca
12841 ccagttttga tttaaacgtg gccaatatgg acaacttctt cgcccccgtt ttcaccatgg
12901 gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg
12961 ccgtttgtga tggcttccat gtcggcagaa tgcttaatga attacaacag tactgcgatg
13021 agtggcaggc ggggcgtaat ctagaggatc cggcttacta aaagccagat aacagtatgc
13081 gtatttgcgc gctgattttt gcggtataag aatatatact gatatgtata cccgaagtat
13141 gtcaaaaaga ggtatgctat gaagcagcgt attacagtga cagttgacag cgacagctat
13201 cagttgctca aggcatatat gatgtcaata tctccggtct ggtaagcaca accatgcaga
13261 atgaagcccg tcgtctgcgt gccgaacgct ggaaagcgga aaatcaggaa gggatggctg
13321 aggtcgcccg gtttattgaa atgaacggct cttttgctga cgagaacagg ggctggtgaa
13381 atgcagttta aggtttacac ctataaaaga gagagccgtt atcgtctgtt tgtggatgta
13441 cagagtgata ttattgacac gcccgggcga cggatggtga tccccctggc cagtgcacgt
13501 ctgctgtcag ataaagtccc ccgtgaactt tacccggtgg tgcatatcgg ggatgaaagc
13561 tggcgcatga tgaccaccga tatggccagt gtgccggtct ccgttatcgg ggaagaagtg
13621 gctgatctca gccaccgcga aaatgacatc aaaaacgcca ttaacctgat gttctgggga
13681 atataaatgt caggctccct tatacacagc cagtctgcag gtcgaccata gtgactggat
13741 atgttgtgtt ttacagtatt atgtagtctg ttttttatgc aaaatctaat ttaatatatt
13801 gatatttata tcattttacg tttctcgttc agctttcttg tacaaagtgg ttcgataatt
13861 cttaattaac tagttctaga gcggccgcca ccgcggtgga gctcgaattt ccccgatcgt
13921 tcaaacattt ggcaataaag tttcttaaga ttgaatcctg ttgccggtct tgcgatgatt
13981 atcatataat ttctgttgaa ttacgttaag catgtaataa ttaacatgta atgcatgacg
14041 ttatttatga gatgggtttt tatgattaga gtcccgcaat tatacattta atacgcgata
14101 gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc atctatgtta
14161 ctagatcggg aatt
//