LOCUS Exported 13277 bp ds-DNA circular SYN 19-DEC-2017
DEFINITION synthetic circular DNA
FEATURES Location/Qualifiers
source 1..13277
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 197..243
/label=minimal CaMV 35S promoter
/note="minimal 35S promoter from cauliflower mosaic virus"
CDS 272..523
/codon_start=1
/gene="lexA (truncated)"
/product="DNA-binding domain of E. coli LexA"
/label=LexA BD
/note="binds the 16-bp palindromic sequence
5'-CTGTATATATATACAG-3'"
/translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAA"
CDS 539..772
/codon_start=1
/gene="UL48"
/product="transcriptional activation domain of herpes
simplex virus protein VP16 (Triezenberg et al., 1988;
Cousens et al., 1989)"
/label=VP16 AD
/translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
CDS 830..1723
/codon_start=1
/gene="ESR1"
/product="estrogen nuclear receptor alpha ligand-binding
domain"
/label=ER-LBD
/translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPGKL
LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
GMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
QKYYITGEAEGFPATV"
promoter 2281..2496
/label=NOS promoter
/note="nopaline synthase promoter"
CDS 2548..3573
/codon_start=1
/label=Hyg
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPGSW
R"
terminator 3611..3863
/label=NOS terminator
/note="nopaline synthase terminator and poly(A) signal"
promoter 4184..4230
/label=minimal CaMV 35S promoter
/note="minimal 35S promoter from cauliflower mosaic virus"
protein_bind 4279..4403
/gene="mutant version of attR"
/label=attR1
/bound_moiety="LR Clonase(TM)"
/note="recombination site for the Gateway(R) LR reaction"
promoter 4428..4458
/label=lac UV5 promoter
/note="E. coli lac promoter with an ""up"" mutation"
CDS 4512..5192
/codon_start=1
/product="chloramphenicol acetyltransferase"
/label=Chl
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
DPAY"
CDS 5512..5817
/codon_start=1
/gene="ccdB"
/product="CcdB, a bacterial toxin that poisons DNA gyrase"
/label=ccdB
/note="Plasmids containing the ccdB gene cannot be
propagated in standard E. coli strains."
/translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
protein_bind complement(5858..5982)
/gene="mutant version of attR"
/label=attR2
/bound_moiety="LR Clonase(TM)"
/note="recombination site for the Gateway(R) LR reaction"
polyA_signal 6499..6673
/label=CaMV poly(A) signal
/note="cauliflower mosaic virus polyadenylation signal"
misc_feature 6751..6775
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA"
CDS 7200..7994
/codon_start=1
/gene="aphA-3"
/product="aminoglycoside phosphotransferase"
/label=Kan
/note="confers resistance to kanamycin"
/translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
rep_origin 8081..8669
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
misc_feature 8855..8995
/label=bom
/note="basis of mobility region from pBR322"
rep_origin 9339..9533
/label=pVS1 oriV
/note="origin of replication for the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
CDS complement(9599..10672)
/codon_start=1
/product="replication protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/label=pVS1 RepA
/translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
LVMRYRNLIEGEASAGS"
CDS complement(11101..11730)
/codon_start=1
/product="stability protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/label=pVS1 StaA
/translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
misc_feature 13031..13055
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
ORIGIN
1 gggcccaaca ctgatagttt aaactgaagg cgggaaacga caatctgatc caagctcaag
61 ctaagcttgc atgcctgcag gatatcgtgg atccaagctt gccacgtgcc gccacgtgcc
121 gccacgtgcc gccacgtgcc tctagaggat ccatctccac tgacgtaagg gatgacgcac
181 aatcccacta tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga
241 ggacacgctg ggatccccaa ttccgggcgg aatgaaagcg ttaacggcca ggcaacaaga
301 ggtgtttgat ctcatccgtg atcacatcag ccagacaggt atgccgccga cgcgtgcgga
361 aatcgcgcag cgtttggggt tccgttcccc aaacgcggct gaagaacatc tgaaggcgct
421 ggcacgcaaa ggcgttattg aaattgtttc cggcgcatca cgcgggattc gtctgttgca
481 ggaagaggaa gaagggttgc cgctggtagg tcgtgtggct gccggtgaac cgtcgagcgc
541 ccccccgacc gatgtcagcc tgggggacga gctccactta gacggcgagg acgtggcgat
601 ggcgcatgcc gacgcgctag acgatttcga tctggacatg ttgggggacg gggattcccc
661 gggtccggga tttacccccc acgactccgc cccctacggc gctctggata tggccgactt
721 cgagtttgag cagatgttta ccgatgccct tggaattgac gagtacggtg gggatccgtc
781 tgctggagac atgagagctg ccaacctttg gccaagcccg ctcatgatca aacgctctaa
841 gaagaacagc ctggccttgt ccctgacggc cgaccagatg gtcagtgcct tgttggatgc
901 tgagcccccc atactctatt ccgagtatga tcctaccaga cccttcagtg aagcttcgat
961 gatgggctta ctgaccaacc tggcagacag ggagctggtt cacatgatca actgggcgaa
1021 gagggtgcca ggctttgtgg atttgaccct ccatgatcag gtccaccttc tagaatgtgc
1081 ctggctagag atcctgatga ttggtctcgt ctggcgctcc atggagcacc cagggaagct
1141 actgtttgct cctaacttgc tcttggacag gaaccaggga aaatgtgtag agggcatggt
1201 ggagatcttc gacatgctgc tggctacatc atctcggttc cgcatgatga atctgcaggg
1261 agaggagttt gtgtgcctca aatctattat tttgcttaat tctggagtgt acacatttct
1321 gtccagcacc ctgaagtctc tggaagagaa ggaccatatc caccgagtcc tggacaagat
1381 cacagacact ttgatccacc tgatggccaa ggcaggcctg accctgcagc agcagcacca
1441 gcggctggcc cagctcctcc tcatcctctc ccacatcagg cacatgagta acaaaggcat
1501 ggagcatctg tacagcatga agtgcaagaa cgtggtgccc ctctatgacc tgctgctgga
1561 gatgctggac gcccaccgcc tacatgcgcc cactagccgt ggaggggcat ccgtggagga
1621 gacggaccaa agccacttgg ccactgcggg ctctacttca tcgcattcct tgcaaaagta
1681 ttacatcacg ggggaggcag agggtttccc tgccacagtc tgagagctcc ctggcggaat
1741 tcccagagat gttagctgaa atcatcacta atcagatacc aaaatattca aatggaaata
1801 tcaaaaagct tctgtttcat caaaaatgac tcgacctaac tgagtaagct agcttgttcg
1861 agtattatgg cattgggaaa actgtttttc ttgtaccatt tgttgtgctt gtaatttact
1921 gtgtttttta ttcggttttc gctatcgaac tgtgaaatgg aaatggatgg agaagagtta
1981 atgaatgata tggtcctttt gttcattctc aaattaatat tatttgtttt ttctcttatt
2041 tgttgtgtgt tgaatttgaa attataagag atatgcaaac attttgtttt gagtaaaaat
2101 gtgtcaaatc gtggcctcta atgaccgaag ttaatatgag gagtaaaaca ctagatcccc
2161 aaacaagctt gaaactgaag gcgggaaacg acaatctgat catgagcgga gaattaaggg
2221 agtcacgtta tgacccccgc cgatgacgcg ggacaagccg ttttacgttt ggaactgaca
2281 gaaccgcaac gttgaaggag ccactcagcc gcgggtttct ggagtttaat gagctaagca
2341 catacgtcag aaaccattat tgcgcgttca aaagtcgcct aaggtcacta tcagctagca
2401 aatatttctt gtcaaaaatg ctccactgac gttccataaa ttcccctcgg tatccaatta
2461 gagtctcata ttcactctca atccaaataa tctgcaccgg atccgctaga ggatctcgac
2521 ctgcaagatc ccggggggca atgagatatg aaaaagcctg aactcaccgc gacgtctgtc
2581 gagaagtttc tgatcgaaaa gttcgacagc gtctccgacc tgatgcagct ctcggagggc
2641 gaagaatctc gtgctttcag cttcgatgta ggagggcgtg gatatgtcct gcgggtaaat
2701 agctgcgccg atggtttcta caaagatcgt tatgtttatc ggcactttgc atcggccgcg
2761 ctcccgattc cggaagtgct tgacattggg gaattcagcg agagcctgac ctattgcatc
2821 tcccgccgtg cacagggtgt cacgttgcaa gacctgcctg aaaccgaact gcccgctgtt
2881 ctgcagccgg tcgcggaggc catggatgcg atcgctgcgg ccgatcttag ccagacgagc
2941 gggttcggcc cattcggacc gcaaggaatc ggtcaataca ctacatggcg tgatttcata
3001 tgcgcgattg ctgatcccca tgtgtatcac tggcaaactg tgatggacga caccgtcagt
3061 gcgtccgtcg cgcaggctct cgatgagctg atgctttggg ccgaggactg ccccgaagtc
3121 cggcacctcg tgcacgcgga tttcggctcc aacaatgtcc tgacggacaa tggccgcata
3181 acagcggtca ttgactggag cgaggcgatg ttcggggatt cccaatacga ggtcgccaac
3241 atcttcttct ggaggccgtg gttggcttgt atggagcagc agacgcgcta cttcgagcgg
3301 aggcatccgg agcttgcagg atcgccgcgg ctccgggcgt atatgctccg cattggtctt
3361 gaccaactct atcagagctt ggttgacggc aatttcgatg atgcagcttg ggcgcagggt
3421 cgatgcgacg caatcgtccg atccggagcc gggactgtcg ggcgtacaca aatcgcccgc
3481 agaagcgcgg ccgtctggac cgatggctgt gtagaagtac tcgccgatag tggaaaccga
3541 cgccccagca ctcgtccggg atcttggagg tgatgtaaca tgatcacaag ctgatccccc
3601 gaatttcccc gatcgttcaa acatttggca ataaagtttc ttaagattga atcctgttgc
3661 cggtcttgcg atgattatca tataatttct gttgaattac gttaagcatg taataattaa
3721 catgtaatgc atgacgttat ttatgagatg ggtttttatg attagagtcc cgcaattata
3781 catttaatac gcgatagaaa acaaaatata gcgcgcaaac taggataaat tatcgcgcgc
3841 ggtgtcatct atgttactag atcgggaatt gatcccccct cgacagcttg catgccgctt
3901 gggctgcagg tcgaggctaa aaaactaatc gcattatcat cccctcgacg tactgtacat
3961 ataaccactg gttttatata cagcagtact gtacatataa ccactggttt tatatacagc
4021 agtcgacgta ctgtacatat aaccactggt tttatataca gcagtactgt acatataacc
4081 actggtttta tatacagcag tcgaggtaag attagatatg gatatgtata tggatatgta
4141 tatggtggta atgccatgta atatgctcga ctctaggatc ttcgcaagac ccttcctcta
4201 tataaggaag ttcatttcat ttggagagga cacgctgaag ctagtcgact ctagcctcga
4261 ggcgcgccaa gctatcaaac aagtttgtac aaaaaagctg aacgagaaac gtaaaatgat
4321 ataaatatca atatattaaa ttagattttg cataaaaaac agactacata atactgtaaa
4381 acacaacata tccagtcact atggcggccg cattaggcac cccaggcttt acactttatg
4441 cttccggctc gtataatgtg tggattttga gttaggatcc gtcgagattt tcaggagcta
4501 aggaagctaa aatggagaaa aaaatcactg gatataccac cgttgatata tcccaatggc
4561 atcgtaaaga acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg
4621 ttcagctgga tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc
4681 cggcctttat tcacattctt gcccgcctga tgaatgctca tccggaattc cgtatggcaa
4741 tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc gttttccatg
4801 agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc cggcagtttc
4861 tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag
4921 ggtttattga gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc accagttttg
4981 atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg ggcaaatatt
5041 atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat gccgtttgtg
5101 atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat gagtggcagg
5161 cggggcgtaa tctagaggat ccggcttact aaaagccaga taacagtatg cgtatttgcg
5221 cgctgatttt tgcggtataa gaatatatac tgatatgtat acccgaagta tgtcaaaaag
5281 aggtatgcta tgaagcagcg tattacagtg acagttgaca gcgacagcta tcagttgctc
5341 aaggcatata tgatgtcaat atctccggtc tggtaagcac aaccatgcag aatgaagccc
5401 gtcgtctgcg tgccgaacgc tggaaagcgg aaaatcagga agggatggct gaggtcgccc
5461 ggtttattga aatgaacggc tcttttgctg acgagaacag gggctggtga aatgcagttt
5521 aaggtttaca cctataaaag agagagccgt tatcgtctgt ttgtggatgt acagagtgat
5581 attattgaca cgcccgggcg acggatggtg atccccctgg ccagtgcacg tctgctgtca
5641 gataaagtcc cccgtgaact ttacccggtg gtgcatatcg gggatgaaag ctggcgcatg
5701 atgaccaccg atatggccag tgtgccggtc tccgttatcg gggaagaagt ggctgatctc
5761 agccaccgcg aaaatgacat caaaaacgcc attaacctga tgttctgggg aatataaatg
5821 tcaggctccc ttatacacag ccagtctgca ggtcgaccat agtgactgga tatgttgtgt
5881 tttacagtat tatgtagtct gttttttatg caaaatctaa tttaatatat tgatatttat
5941 atcattttac gtttctcgtt cagctttctt gtacaaagtg gttcgataat tcttaattaa
6001 ctagtcgatc caggcctccc agctttcgtc cgtatcatcg gtttcgacaa cgttcgtcaa
6061 gttcaatgca tcagtttcat tgcccacaca ccagaatcct actaagtttg agtattatgg
6121 cattggaaaa gctgttttct tctatcattt gttctgcttg taatttactg tgttctttca
6181 gtttttgttt tcggacatca aaatgcaaat ggatggataa gagttaataa atgatatggt
6241 ccttttgttc attctcaaat tattattatc tgttgttttt actttaatgg gttgaattta
6301 agtaagaaag gaactaacag tgtgatatta aggtgcaatg ttagacatat aaaacagtct
6361 ttcacctctc tttggttatg tcttgaattg gtttgtttct tcacttatct gtgtaatcaa
6421 gtttactatg agtctatgat caagtaatta tgcaatcaag ttaagtacag tataggcttt
6481 ttgtgtcgag ggggtacctt tctccataat aatgtgtgag tagttcccag ataagggaat
6541 tagggttcct atagggtttc gctcatgtgt tgagcatata agaaaccctt agtatgtatt
6601 tgtatttgta aaatacttct atcaataaaa tttctaattc ctaaaaccaa aatccagtac
6661 taaaatccag atcccccgaa ttaattcggc gttaattcag tacattaaaa acgtccgcaa
6721 tgtgttatta agttgtctaa gcgtcaattt gtttacacca caatatatcc tgccaccagc
6781 cagccaacag ctccccgacc ggcagctcgg cacaaaatca ccactcgata caggcagccc
6841 atcagtccgg gacggcgtca gcgggagagc cgttgtaagg cggcagactt tgctcatgtt
6901 accgatgcta ttcggaagaa cggcaactaa gctgccgggt ttgaaacacg gatgatctcg
6961 cggagggtag catgttgatt gtaacgatga cagagcgttg ctgcctgtga tcaccgcggt
7021 ttcaaaatcg gctccgtcga tactatgtta tacgccaact ttgaaaacaa ctttgaaaaa
7081 gctgttttct ggtatttaag gttttagaat gcaaggaaca gtgaattgga gttcgtcttg
7141 ttataattag cttcttgggg tatctttaaa tactgtagaa aagaggaagg aaataataaa
7201 tggctaaaat gagaatatca ccggaattga aaaaactgat cgaaaaatac cgctgcgtaa
7261 aagatacgga aggaatgtct cctgctaagg tatataagct ggtgggagaa aatgaaaacc
7321 tatatttaaa aatgacggac agccggtata aagggaccac ctatgatgtg gaacgggaaa
7381 aggacatgat gctatggctg gaaggaaagc tgcctgttcc aaaggtcctg cactttgaac
7441 ggcatgatgg ctggagcaat ctgctcatga gtgaggccga tggcgtcctt tgctcggaag
7501 agtatgaaga tgaacaaagc cctgaaaaga ttatcgagct gtatgcggag tgcatcaggc
7561 tctttcactc catcgacata tcggattgtc cctatacgaa tagcttagac agccgcttag
7621 ccgaattgga ttacttactg aataacgatc tggccgatgt ggattgcgaa aactgggaag
7681 aagacactcc atttaaagat ccgcgcgagc tgtatgattt tttaaagacg gaaaagcccg
7741 aagaggaact tgtcttttcc cacggcgacc tgggagacag caacatcttt gtgaaagatg
7801 gcaaagtaag tggctttatt gatcttggga gaagcggcag ggcggacaag tggtatgaca
7861 ttgccttctg cgtccggtcg atcagggagg atatcgggga agaacagtat gtcgagctat
7921 tttttgactt actggggatc aagcctgatt gggagaaaat aaaatattat attttactgg
7981 atgaattgtt ttagtaccta gaatgcatga ccaaaatccc ttaacgtgag ttttcgttcc
8041 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
8101 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
8161 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
8221 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
8281 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
8341 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
8401 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
8461 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
8521 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
8581 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
8641 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
8701 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
8761 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
8821 gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc
8881 atctgtgcgg tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg
8941 catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg
9001 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta
9061 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc
9121 gaaacgcgcg aggcagggtg ccttgatgtg ggcgccggcg gtcgagtggc gacggcgcgg
9181 cttgtccgcg ccctggtaga ttgcctggcc gtaggccagc catttttgag cggccagcgg
9241 ccgcgatagg ccgacgcgaa gcggcggggc gtagggagcg cagcgaccga agggtaggcg
9301 ctttttgcag ctcttcggct gtgcgctggc cagacagtta tgcacaggcc aggcgggttt
9361 taagagtttt aataagtttt aaagagtttt aggcggaaaa atcgcctttt ttctctttta
9421 tatcagtcac ttacatgtgt gaccggttcc caatgtacgg ctttgggttc ccaatgtacg
9481 ggttccggtt cccaatgtac ggctttgggt tcccaatgta cgtgctatcc acaggaaaga
9541 gaccttttcg acctttttcc cctgctaggg caatttgccc tagcatctgc tccgtacatt
9601 aggaaccggc ggatgcttcg ccctcgatca ggttgcggta gcgcatgact aggatcgggc
9661 cagcctgccc cgcctcctcc ttcaaatcgt actccggcag gtcatttgac ccgatcagct
9721 tgcgcacggt gaaacagaac ttcttgaact ctccggcgct gccactgcgt tcgtagatcg
9781 tcttgaacaa ccatctggct tctgccttgc ctgcggcgcg gcgtgccagg cggtagagaa
9841 aacggccgat gccgggatcg atcaaaaagt aatcggggtg aaccgtcagc acgtccgggt
9901 tcttgccttc tgtgatctcg cggtacatcc aatcagctag ctcgatctcg atgtactccg
9961 gccgcccggt ttcgctcttt acgatcttgt agcggctaat caaggcttca ccctcggata
10021 ccgtcaccag gcggccgttc ttggccttct tcgtacgctg catggcaacg tgcgtggtgt
10081 ttaaccgaat gcaggtttct accaggtcgt ctttctgctt tccgccatcg gctcgccggc
10141 agaacttgag tacgtccgca acgtgtggac ggaacacgcg gccgggcttg tctcccttcc
10201 cttcccggta tcggttcatg gattcggtta gatgggaaac cgccatcagt accaggtcgt
10261 aatcccacac actggccatg ccggccggcc ctgcggaaac ctctacgtgc ccgtctggaa
10321 gctcgtagcg gatcacctcg ccagctcgtc ggtcacgctt cgacagacgg aaaacggcca
10381 cgtccatgat gctgcgacta tcgcgggtgc ccacgtcata gagcatcgga acgaaaaaat
10441 ctggttgctc gtcgcccttg ggcggcttcc taatcgacgg cgcaccggct gccggcggtt
10501 gccgggattc tttgcggatt cgatcagcgg ccgcttgcca cgattcaccg gggcgtgctt
10561 ctgcctcgat gcgttgccgc tgggcggcct gcgcggcctt caacttctcc accaggtcat
10621 cacccagcgc cgcgccgatt tgtaccgggc cggatggttt gcgaccgctc acgccgattc
10681 ctcgggcttg ggggttccag tgccattgca gggccggcag gcaacccagc cgcttacgcc
10741 tggccaaccg cccgttcctc cacacatggg gcattccacg gcgtcggtgc ctggttgttc
10801 ttgattttcc atgccgcctc ctttagccgc taaaattcat ctactcattt attcatttgc
10861 tcatttactc tggtagctgc gcgatgtatt cagatagcag ctcggtaatg gtcttgcctt
10921 ggcgtaccgc gtacatcttc agcttggtgt gatcctccgc cggcaactga aagttgaccc
10981 gcttcatggc tggcgtgtct gccaggctgg ccaacgttgc agccttgctg ctgcgtgcgc
11041 tcggacggcc ggcacttagc gtgtttgtgc ttttgctcat tttctcttta cctcattaac
11101 tcaaatgagt tttgatttaa tttcagcggc cagcgcctgg acctcgcggg cagcgtcgcc
11161 ctcgggttct gattcaagaa cggttgtgcc ggcggcggca gtgcctgggt agctcacgcg
11221 ctgcgtgata cgggactcaa gaatgggcag ctcgtacccg gccagcgcct cggcaacctc
11281 accgccgatg cgcgtgcctt tgatcgcccg cgacacgaca aaggccgctt gtagccttcc
11341 atccgtgacc tcaatgcgct gcttaaccag ctccaccagg tcggcggtgg cccatatgtc
11401 gtaagggctt ggctgcaccg gaatcagcac gaagtcggct gccttgatcg cggacacagc
11461 caagtccgcc gcctggggcg ctccgtcgat cactacgaag tcgcgccggc cgatggcctt
11521 cacgtcgcgg tcaatcgtcg ggcggtcgat gccgacaacg gttagcggtt gatcttcccg
11581 cacggccgcc caatcgcggg cactgccctg gggatcggaa tcgactaaca gaacatcggc
11641 cccggcgagt tgcagggcgc gggctagatg ggttgcgatg gtcgtcttgc ctgacccgcc
11701 tttctggtta agtacagcga taaccttcat gcgttcccct tgcgtatttg tttatttact
11761 catcgcatca tatacgcagc gaccgcatga cgcaagctgt tttactcaaa tacacatcac
11821 ctttttagac ggcggcgctc ggtttcttca gcggccaagc tggccggcca ggccgccagc
11881 ttggcatcag acaaaccggc caggatttca tgcagccgca cggttgagac gtgcgcgggc
11941 ggctcgaaca cgtacccggc cgcgatcatc tccgcctcga tctcttcggt aatgaaaaac
12001 ggttcgtcct ggccgtcctg gtgcggtttc atgcttgttc ctcttggcgt tcattctcgg
12061 cggccgccag ggcgtcggcc tcggtcaatg cgtcctcacg gaaggcaccg cgccgcctgg
12121 cctcggtggg cgtcacttcc tcgctgcgct caagtgcgcg gtacagggtc gagcgatgca
12181 cgccaagcag tgcagccgcc tctttcacgg tgcggccttc ctggtcgatc agctcgcggg
12241 cgtgcgcgat ctgtgccggg gtgagggtag ggcgggggcc aaacttcacg cctcgggcct
12301 tggcggcctc gcgcccgctc cgggtgcggt cgatgattag ggaacgctcg aactcggcaa
12361 tgccggcgaa cacggtcaac accatgcggc cggccggcgt ggtggtgtcg gcccacggct
12421 ctgccaggct acgcaggccc gcgccggcct cctggatgcg ctcggcaatg tccagtaggt
12481 cgcgggtgct gcgggccagg cggtctagcc tggtcactgt cacaacgtcg ccagggcgta
12541 ggtggtcaag catcctggcc agctccgggc ggtcgcgcct ggtgccggtg atcttctcgg
12601 aaaacagctt ggtgcagccg gccgcgtgca gttcggcccg ttggttggtc aagtcctggt
12661 cgtcggtgct gacgcgggca tagcccagca ggccagcggc ggcgctcttg ttcatggcgt
12721 aatgtctccg gttctagtcg caagtattct actttatgcg actaaaacac gcgacaagaa
12781 aacgccagga aaagggcagg gcggcagcct gtcgcgtaac ttaggacttg tgcgacatgt
12841 cgttttcaga agacggctgc actgaacgtc agaagccgac tgcactatag cagcggaggg
12901 gttggatcaa agtactttga tcccgagggg aaccctgtgg ttggcatgca catacaaatg
12961 gacgaacgga taaacctttt cacgcccttt taaatatccg attattctaa taaacgctct
13021 tttctcttag gtttacccgc caatatatcc tgtcaaacac tgatagttta aactgaaggc
13081 gggaaacgac aatctgatcc aagctcaagc tgctctagca ttcgccattc aggctgcgca
13141 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg
13201 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta
13261 aaacgacggc cagtgcc
//