LOCUS Exported 12038 bp ds-DNA circular SYN 04-DEC-2017
DEFINITION synthetic circular DNA
FEATURES Location/Qualifiers
source 1..12038
/organism="synthetic DNA construct"
/mol_type="other DNA"
terminator 115..367
/label=NOS terminator
/note="nopaline synthase terminator and poly(A) signal"
CDS 728..1033
/codon_start=1
/gene="ccdB"
/product="CcdB, a bacterial toxin that poisons DNA gyrase"
/label=ccdB
/note="Plasmids containing the ccdB gene cannot be
propagated in standard E. coli strains."
/translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
CDS complement(1089..1115)
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
promoter complement(1131..3121)
/label=Ubi promoter
/note="maize polyubiquitin gene promoter"
misc_feature 3362..3386
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
CDS 4685..5314
/codon_start=1
/product="stability protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/label=pVS1 StaA
/translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
CDS 5748..6815
/codon_start=1
/product="replication protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/label=pVS1 RepA
/translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
MRYRNLIEGEASAGS"
rep_origin 6881..7075
/label=pVS1 oriV
/note="origin of replication for the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
misc_feature 7419..7559
/label=bom
/note="basis of mobility region from pBR322"
rep_origin complement(7745..8333)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(8420..9214)
/codon_start=1
/gene="aphA-3"
/product="aminoglycoside phosphotransferase"
/label=KanR
/note="confers resistance to kanamycin"
/translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
misc_feature 9639..9663
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA"
polyA_signal 9741..9915
/label=CaMV poly(A) signal
/note="cauliflower mosaic virus polyadenylation signal"
CDS complement(9955..10980)
/codon_start=1
/gene="aph(4)-Ia"
/product="aminoglycoside phosphotransferase from E. coli"
/label=HygR
/note="confers resistance to hygromycin"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
K"
promoter complement(11047..11724)
/label=CaMV 35S promoter (enhanced)
/note="cauliflower mosaic virus 35S promoter with a
duplicated enhancer region"
ORIGIN
1 gaattcgagc tcggtacccc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt
61 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgaat tcccgatcta
121 gtaacataga tgacaccgcg cgcgataatt tatcctagtt tgcgcgctat attttgtttt
181 ctatcgcgta ttaaatgtat aattgcggga ctctaatcat aaaaacccat ctcataaata
241 acgtcatgca ttacatgtta attattacat gcttaacgta attcaacaga aattatatga
301 taatcatcgc aagaccggca acaggattca atcttaagaa actttattgc caaatgtttg
361 aacgatcggg gaaattcgga tccccaatac ttgtatgggg cttactaaaa gccagataac
421 agtatgcgta tttgcgcgct gatttttgcg gtataagaat atatactgat atgtataccc
481 gaagtatgtc aaaaagaggt atgctatgaa gcagcgtatt acagtgacag ttgacagcga
541 cagctatcag ttgctcaagg catatatgat gtcaatatct ccggtctggt aagcacaacc
601 atgcagaatg aagcccgtcg tctgcgtgcc gaacgctgga aagcggaaaa tcaggaaggg
661 atggctgagg tcgcccggtt tattgaaatg aacggctctt ttgctgacga gaacaggggc
721 tggtgaaatg cagtttaagg tttacaccta taaaagagag agccgttatc gtctgtttgt
781 ggatgtacag agtgatatta ttgacacgcc cgggcgacgg atggtgatcc ccctggccag
841 tgcacgtctg ctgtcagata aagtctcccg tgaactttac ccggtggtgc atatcgggga
901 tgaaagctgg cgcatgatga ccaccgatat ggccagtgtg ccggtctccg ttatcgggga
961 agaagtggct gatctcagcc accgcgaaaa tgacatcaaa aacgccatta acctgatgtt
1021 ctggggaata taaatgtcag gctcccttat acacagccag tctgcaggtc gacccataca
1081 agtattggag cgtaatctgg aacatcgtat gggtacatgg atcccccggg ctgcagaagt
1141 aacaccaaac aacagggtga gcatcgacaa aagaaacagt accaagcaaa taaatagcgt
1201 atgaaggcag ggctaaaaaa atccacatat agctgctgca tatgccatca tccaagtata
1261 tcaagatcaa aataattata aaacatactt gtttattata atagataggt actcaaggtt
1321 agagcatatg aatagatgct gcatatgcca tcatgtatat gcatcagtaa aacccacatc
1381 aacatgtata cctatcctag atcgatattt ccatccatct taaactcgta actatgaaga
1441 tgtatgacac acacatacag ttccaaaatt aataaataca ccaggtagtt tgaaacagta
1501 ttctactccg atctagaacg aatgaacgac cgcccaacca caccacatca tcacaaccaa
1561 gcgaacaaaa agcatctctg tatatgcatc agtaaaaccc gcatcaacat gtatacctat
1621 cctagatcga tatttccatc catcatcttc aattcgtaac tatgaatatg tatggcacac
1681 acatacagat ccaaaattaa taaatccacc aggtagtttg aaacagaatt ctactccgat
1741 ctagaacgac cgcccaacca gaccacatca tcacaaccaa gacaaaaaaa agcatgaaaa
1801 gatgacccga caaacaagtg cacggcatat attgaaataa aggaaaaggg caaaccaaac
1861 cctatgcaac gaaacaaaaa aaatcatgaa atcgatcccg tctgcggaac ggctagagcc
1921 atcccaggat tccccaaaga gaaacactgg caagttagca atcagaacgt gtctgacgta
1981 caggtcgcat ccgtgtacga acgctagcag cacggatcta acacaaacac ggatctaaca
2041 caaacatgaa cagaagtaga actaccgggc cctaaccatg gaccggaacg ccgatctaga
2101 gaaggtagag aggggggggg ggggaggacg agcggcgtac cttgaagcgg aggtgccgac
2161 gggtggattt gggggagatc tggttgtgtg tgtgtgcgct ccgaacaaca cgaggttggg
2221 gaaagagggt gtggaggggg tgtctattta ttacggcggg cgaggaaggg aaagcgaagg
2281 agcggtggga aaggaatccc ccgtagctgc cgtgccgtga gaggaggagg aggccgcctg
2341 ccgtgccggc tcacgtctgc cgctccgcca cgcatttctg gatgccgaca gcggagcaag
2401 tccaacggtg gagcggaact ctcgagaggg gtccagaggc agcgacagag atgccgtgcc
2461 gtctgcttcg cttggcccga cgcgacgctg ctggttcgct ggttggtgtc cgttagactc
2521 gtcgacggcg tttaacaggc tggcattatc tactcgaaac aagaaaaatg tttccttagt
2581 ttttttaatt tcttaaaggg tatttgttta atttttagtc actttatttt attctatttt
2641 atatctaaat tattaaataa aaaaactaaa atagagtttt agttttctta atttagaggc
2701 taaaatagaa taaaatagat gtactaaaaa aattagtcta taaaaaccat taaccctaaa
2761 ccctaaatgg atgtactaat aaaatggatg aagtattata taggtgaagc tatttgcaaa
2821 aaaaaaggag aacacatgca cactaaaaag ataaaactgt agagtcctgt tgtcaaaata
2881 ctcaattgtc ctttagacca tgtctaactg ttcatttata tgattctcta aaacactgat
2941 attattgtag tactatagat tatattattc gtagagtaaa gtttaaatat atgtataaag
3001 atagataaac tgcacttcaa acaagtgtga caaaaaaaat atgtggtaat tttttataac
3061 ttagacatgc aatgctcatt atctctagag aggggcacga ccgggtcacg ctgcactgca
3121 ggaattcgat atcaagcttg gcactggccg tcgttttaca acgtcgtgac tgggaaaacc
3181 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
3241 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatgct
3301 agagcagctt gagcttggat cagattgtcg tttcccgcct tcagtttaaa ctatcagtgt
3361 ttgacaggat atattggcgg gtaaacctaa gagaaaagag cgtttattag aataacggat
3421 atttaaaagg gcgtgaaaag gtttatccgt tcgtccattt gtatgtgcat gccaaccaca
3481 gggttcccct cgggatcaaa gtactttgat ccaacccctc cgctgctata gtgcagtcgg
3541 cttctgacgt tcagtgcagc cgtcttctga aaacgacatg tcgcacaagt cctaagttac
3601 gcgacaggct gccgccctgc ccttttcctg gcgttttctt gtcgcgtgtt ttagtcgcat
3661 aaagtagaat acttgcgact agaaccggag acattacgcc atgaacaaga gcgccgccgc
3721 tggcctgctg ggctatgccc gcgtcagcac cgacgaccag gacttgacca accaacgggc
3781 cgaactgcac gcggccggct gcaccaagct gttttccgag aagatcaccg gcaccaggcg
3841 cgaccgcccg gagctggcca ggatgcttga ccacctagcc ctggcgacgt tgtgacagtg
3901 accaggctag accgcctggc ccgcagcacc cgcgacctac tggacattgc cgagcgcatc
3961 caggaggccg gcgcgggcct gcgtagcctg gcagagccgt gggccgacac caccacgccg
4021 gccggccgca tggtgttgac cgtgttcgcc ggcattgccg agttcgagcg ttccctaatc
4081 atcgaccgca cccggagcgg gcgcgaggcc gccaaggccc gaggcgtgaa gtttggcccc
4141 cgccctaccc tcaccccggc acagatcgcg cacgcccgcg agctgatcga ccaggaaggc
4201 cgcaccgtga aagaggcggc tgcactgctt ggcgtgcatc gctcgaccct gtaccgcgca
4261 cttgagcgca gcgaggaagt gacgcccacc gaggccaggc ggcgcggtgc cttccgtgag
4321 gacgcattga ccgaggccga cgccctggcg gccgccgaga atgaacgcca agaggaacaa
4381 gcatgaaacc gcaccaggac ggccaggacg aaccgttttt cattaccgaa gagatcgagg
4441 cggagatgat cgcggccggg tacgtgttcg agccgcccgc gcacgtctca accgtgcggc
4501 tgcatgaaat cctggccggt ttgtctgatg ccaagctggc ggcctggccg gccagcttgg
4561 ccgctgaaga aaccgagcgc cgccgtctaa aaaggtgatg tgtatttgag taaaacagct
4621 tgcgtcatgc ggtcgctgcg tatatgatgc gatgagtaaa taaacaaata cgcaagggga
4681 acgcatgaag gttatcgctg tacttaacca gaaaggcggg tcaggcaaga cgaccatcgc
4741 aacccatcta gcccgcgccc tgcaactcgc cggggccgat gttctgttag tcgattccga
4801 tccccagggc agtgcccgcg attgggcggc cgtgcgggaa gatcaaccgc taaccgttgt
4861 cggcatcgac cgcccgacga ttgaccgcga cgtgaaggcc atcggccggc gcgacttcgt
4921 agtgatcgac ggagcgcccc aggcggcgga cttggctgtg tccgcgatca aggcagccga
4981 cttcgtgctg attccggtgc agccaagccc ttacgacata tgggcaaccg ccgacctggt
5041 ggagctggtt aagcagcgca ttgaggtcac ggatggaagg ctacaagcgg cctttgtcgt
5101 gtcgcgggcg atcaaaggca cgcgcatcgg cggtgaggtt gccgaggcgc tggccgggta
5161 cgagctgccc attcttgagt cccgtatcac gcagcgcgtg agctacccag gcactgccgc
5221 cgccggcaca accgttcttg aatcagaacc cgagggcgac gctgcccgcg aggtccaggc
5281 gctggccgct gaaattaaat caaaactcat ttgagttaat gaggtaaaga gaaaatgagc
5341 aaaagcacaa acacgctaag tgccggccgt ccgagcgcac gcagcagcaa ggctgcaacg
5401 ttggccagcc tggcagacac gccagccatg aagcgggtca actttcagtt gccggcggag
5461 gatcacacca agctgaagat gtacgcggta cgccaaggca agaccattac cgagctgcta
5521 tctgaataca tcgcgcagct accagagtaa atgagcaaat gaataaatga gtagatgaat
5581 tttagcggct aaaggaggcg gcatggaaaa tcaagaacaa ccaggcaccg acgccgtgga
5641 atgccccatg tgtggaggaa cgggcggttg gccaggcgta agcggctggg ttgtctgccg
5701 gccctgcaat ggcactggaa cccccaagcc cgaggaatcg gcgtgacggt cgcaaaccat
5761 ccggcccggt acaaatcggc gcggcgctgg gtgatgacct ggtggagaag ttgaaggccg
5821 cgcaggccgc ccagcggcaa cgcatcgagg cagaagcacg ccccggtgaa tcgtggcaag
5881 cggccgctga tcgaatccgc aaagaatccc ggcaaccgcc ggcagccggt gcgccgtcga
5941 ttaggaagcc gcccaagggc gacgagcaac cagatttttt cgttccgatg ctctatgacg
6001 tgggcacccg cgatagtcgc agcatcatgg acgtggccgt tttccgtctg tcgaagcgtg
6061 accgacgagc tggcgaggtg atccgctacg agcttccaga cgggcacgta gaggtttccg
6121 cagggccggc cggcatggcc agtgtgtggg attacgacct ggtactgatg gcggtttccc
6181 atctaaccga atccatgaac cgataccggg aagggaaggg agacaagccc ggccgcgtgt
6241 tccgtccaca cgttgcggac gtactcaagt tctgccggcg agccgatggc ggaaagcaga
6301 aagacgacct ggtagaaacc tgcattcggt taaacaccac gcacgttgcc atgcagcgta
6361 cgaagaaggc caagaacggc cgcctggtga cggtatccga gggtgaagcc ttgattagcc
6421 gctacaagat cgtaaagagc gaaaccgggc ggccggagta catcgagatc gagctagctg
6481 attggatgta ccgcgagatc acagaaggca agaacccgga cgtgctgacg gttcaccccg
6541 attacttttt gatcgatccc ggcatcggcc gttttctcta ccgcctggca cgccgcgccg
6601 caggcaaggc agaagccaga tggttgttca agacgatcta cgaacgcagt ggcagcgccg
6661 gagagttcaa gaagttctgt ttcaccgtgc gcaagctgat cgggtcaaat gacctgccgg
6721 agtacgattt gaaggaggag gcggggcagg ctggcccgat cctagtcatg cgctaccgca
6781 acctgatcga gggcgaagca tccgccggtt cctaatgtac ggagcagatg ctagggcaaa
6841 ttgccctagc aggggaaaaa ggtcgaaaag gtctctttcc tgtggatagc acgtacattg
6901 ggaacccaaa gccgtacatt gggaaccgga acccgtacat tgggaaccca aagccgtaca
6961 ttgggaaccg gtcacacatg taagtgactg atataaaaga gaaaaaaggc gatttttccg
7021 cctaaaactc tttaaaactt attaaaactc ttaaaacccg cctggcctgt gcataactgt
7081 ctggccagcg cacagccgaa gagctgcaaa aagcgcctac ccttcggtcg ctgcgctccc
7141 tacgccccgc cgcttcgcgt cggcctatcg cggccgctgg ccgctcaaaa atggctggcc
7201 tacggccagg caatctacca gggcgcggac aagccgcgcc gtcgccactc gaccgccggc
7261 gcccacatca aggcaccctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
7321 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
7381 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt
7441 agcgatagcg gagtgtatac tggcttaact atgcggcatc agagcagatt gtactgagag
7501 tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc
7561 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
7621 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
7681 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
7741 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
7801 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
7861 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
7921 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
7981 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
8041 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
8101 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
8161 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
8221 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
8281 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
8341 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
8401 tggtcatgca ttctaggtac taaaacaatt catccagtaa aatataatat tttattttct
8461 cccaatcagg cttgatcccc agtaagtcaa aaaatagctc gacatactgt tcttccccga
8521 tatcctccct gatcgaccgg acgcagaagg caatgtcata ccacttgtcc gccctgccgc
8581 ttctcccaag atcaataaag ccacttactt tgccatcttt cacaaagatg ttgctgtctc
8641 ccaggtcgcc gtgggaaaag acaagttcct cttcgggctt ttccgtcttt aaaaaatcat
8701 acagctcgcg cggatcttta aatggagtgt cttcttccca gttttcgcaa tccacatcgg
8761 ccagatcgtt attcagtaag taatccaatt cggctaagcg gctgtctaag ctattcgtat
8821 agggacaatc cgatatgtcg atggagtgaa agagcctgat gcactccgca tacagctcga
8881 taatcttttc agggctttgt tcatcttcat actcttccga gcaaaggacg ccatcggcct
8941 cactcatgag cagattgctc cagccatcat gccgttcaaa gtgcaggacc tttggaacag
9001 gcagctttcc ttccagccat agcatcatgt ccttttcccg ttcaacatca taggtggtcc
9061 ctttataccg gctgtccgtc atttttaaat ataggttttc attttctccc accagcttat
9121 ataccttagc aggagacatt ccttccgtat cttttacgca gcggtatttt tcgatcagtt
9181 ttttcaattc cggtgatatt ctcattttag ccatttatta tttccttcct cttttctaca
9241 gtatttaaag ataccccaag aagctaatta taacaagacg aactccaatt cactgttcct
9301 tgcattctaa aaccttaaat accagaaaac agctttttca aagttgtttt caaagttggc
9361 gtataacata gtatcgacgg agccgatttt gaaaccgcgg tgatcacagg cagcaacgct
9421 ctgtcatcgt tacaatcaac atgctaccct ccgcgagatc atccgtgttt caaacccggc
9481 agcttagttg ccgttcttcc gaatagcatc ggtaacatga gcaaagtctg ccgccttaca
9541 acggctctcc cgctgacgcc gtcccggact gatgggctgc ctgtatcgag tggtgatttt
9601 gtgccgagct gccggtcggg gagctgttgg ctggctggtg gcaggatata ttgtggtgta
9661 aacaaattga cgcttagaca acttaataac acattgcgga cgtttttaat gtactgaatt
9721 aacgccgaat taattcgggg gatctggatt ttagtactgg attttggttt taggaattag
9781 aaattttatt gatagaagta ttttacaaat acaaatacat actaagggtt tcttatatgc
9841 tcaacacatg agcgaaaccc tataggaacc ctaattccct tatctgggaa ctactcacac
9901 attattatgg agaaactcga gcttgtcgat cgacagatcc ggtcggcatc tactctattt
9961 ctttgccctc ggacgagtgc tggggcgtcg gtttccacta tcggcgagta cttctacaca
10021 gccatcggtc cagacggccg cgcttctgcg ggcgatttgt gtacgcccga cagtcccggc
10081 tccggatcgg acgattgcgt cgcatcgacc ctgcgcccaa gctgcatcat cgaaattgcc
10141 gtcaaccaag ctctgataga gttggtcaag accaatgcgg agcatatacg cccggagtcg
10201 tggcgatcct gcaagctccg gatgcctccg ctcgaagtag cgcgtctgct gctccataca
10261 agccaaccac ggcctccaga agaagatgtt ggcgacctcg tattgggaat ccccgaacat
10321 cgcctcgctc cagtcaatga ccgctgttat gcggccattg tccgtcagga cattgttgga
10381 gccgaaatcc gcgtgcacga ggtgccggac ttcggggcag tcctcggccc aaagcatcag
10441 ctcatcgaga gcctgcgcga cggacgcact gacggtgtcg tccatcacag tttgccagtg
10501 atacacatgg ggatcagcaa tcgcgcatat gaaatcacgc catgtagtgt attgaccgat
10561 tccttgcggt ccgaatgggc cgaacccgct cgtctggcta agatcggccg cagcgatcgc
10621 atccatagcc tccgcgaccg gttgtagaac agcgggcagt tcggtttcag gcaggtcttg
10681 caacgtgaca ccctgtgcac ggcgggagat gcaataggtc aggctctcgc taaactcccc
10741 aatgtcaagc acttccggaa tcgggagcgc ggccgatgca aagtgccgat aaacataacg
10801 atctttgtag aaaccatcgg cgcagctatt tacccgcagg acatatccac gccctcctac
10861 atcgaagctg aaagcacgag attcttcgcc ctccgagagc tgcatcaggt cggagacgct
10921 gtcgaacttt tcgatcagaa acttctcgac agacgtcgcg gtgagttcag gctttttcat
10981 atctcattgc cccccggatc tgcgaaagct cgagagagat agatttgtag agagagactg
11041 gtgatttcag cgtgtcctct ccaaatgaaa tgaacttcct tatatagagg aaggtcttgc
11101 gaaggatagt gggattgtgc gtcatccctt acgtcagtgg agatatcaca tcaatccact
11161 tgctttgaag acgtggttgg aacgtcttct ttttccacga tgctcctcgt gggtgggggt
11221 ccatctttgg gaccactgtc ggcagaggca tcttgaacga tagcctttcc tttatcgcaa
11281 tgatggcatt tgtaggtgcc accttccttt tctactgtcc ttttgatgaa gtgacagata
11341 gctgggcaat ggaatccgag gaggtttccc gatattaccc tttgttgaaa agtctcaata
11401 gccctttggt cttctgagac tgtatctttg atattcttgg agtagacgag agtgtcgtgc
11461 tccaccatgt tatcacatca atccacttgc tttgaagacg tggttggaac gtcttctttt
11521 tccacgatgc tcctcgtggg tgggggtcca tctttgggac cactgtcggc agaggcatct
11581 tgaacgatag cctttccttt atcgcaatga tggcatttgt aggtgccacc ttccttttct
11641 actgtccttt tgatgaagtg acagatagct gggcaatgga atccgaggag gtttcccgat
11701 attacccttt gttgaaaagt ctcaatagcc ctttggtctt ctgagactgt atctttgata
11761 ttcttggagt agacgagagt gtcgtgctcc accatgttgg caagctgctc tagccaatac
11821 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
11881 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
11941 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
12001 aacaatttca cacaggaaac agctatgacc atgattac
//