LOCUS Exported 10835 bp ds-DNA circular SYN 30-OCT-2017
DEFINITION synthetic circular DNA
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10835)
TITLE Direct Submission
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..10835
/organism="synthetic DNA construct"
/mol_type="other DNA"
terminator 7..270
/label=NOS terminator
/note="nopaline synthase terminator and poly(A) signal"
CDS 627..932
/codon_start=1
/gene="ccdB"
/product="CcdB, a bacterial toxin that poisons DNA gyrase"
/label=ccdB
/note="Plasmids containing the ccdB gene cannot be
propagated in standard E. coli strains."
/translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
CDS complement(988..1017)
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
promoter complement(1035..1925)
/label=CaMV 35S promoter
/note="strong constitutive promoter from cauliflower mosaic
virus"
misc_feature 2159..2183
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
CDS 3482..4111
/codon_start=1
/product="stability protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/label=pVS1 StaA
/translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
CDS 4545..5612
/codon_start=1
/product="replication protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/label=pVS1 RepA
/translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
MRYRNLIEGEASAGS"
rep_origin 5678..5872
/label=pVS1 oriV
/note="origin of replication for the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
misc_feature 6216..6356
/label=bom
/note="basis of mobility region from pBR322"
rep_origin complement(6542..7130)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(7217..8011)
/codon_start=1
/gene="aphA-3"
/product="aminoglycoside phosphotransferase"
/label=KanR
/note="confers resistance to kanamycin"
/translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
misc_feature 8436..8460
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA"
polyA_signal 8538..8712
/label=CaMV poly(A) signal
/note="cauliflower mosaic virus polyadenylation signal"
CDS complement(8752..9777)
/codon_start=1
/gene="aph(4)-Ia"
/product="aminoglycoside phosphotransferase from E. coli"
/label=HygR
/note="confers resistance to hygromycin"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
K"
promoter complement(9844..10521)
/label=CaMV 35S promoter (enhanced)
/note="cauliflower mosaic virus 35S promoter with a
duplicated enhancer region"
ORIGIN
1 gaattccgat ctagtaacat agatgacacc gcgcgcgata atttatccta gtttgcgcgc
61 tatattttgt tttctatcgc gtattaaatg tataattgcg ggactctaat cataaaaacc
121 catctcataa ataacgtcat gcattacatg ttaattatta catgcttaac gtaattcaac
181 agaaattata tgataatcat cgcaagaccg gcaacaggat tcaatcttaa gaaactttat
241 tgccaaatgt ttgaacgatc ggggaaattc gctagtggat ccccaatact tgtatggggc
301 ttactaaaag ccagataaca gtatgcgtat ttgcgcgctg atttttgcgg tataagaata
361 tatactgata tgtatacccg aagtatgtca aaaagaggta tgctatgaag cagcgtatta
421 cagtgacagt tgacagcgac agctatcagt tgctcaaggc atatatgatg tcaatatctc
481 cggtctggta agcacaacca tgcagaatga agcccgtcgt ctgcgtgccg aacgctggaa
541 agcggaaaat caggaaggga tggctgaggt cgcccggttt attgaaatga acggctcttt
601 tgctgacgag aacaggggct ggtgaaatgc agtttaaggt ttacacctat aaaagagaga
661 gccgttatcg tctgtttgtg gatgtacaga gtgatattat tgacacgccc gggcgacgga
721 tggtgatccc cctggccagt gcacgtctgc tgtcagataa agtctcccgt gaactttacc
781 cggtggtgca tatcggggat gaaagctggc gcatgatgac caccgatatg gccagtgtgc
841 cggtctccgt tatcggggaa gaagtggctg atctcagcca ccgcgaaaat gacatcaaaa
901 acgccattaa cctgatgttc tggggaatat aaatgtcagg ctcccttata cacagccagt
961 ctgcaggtcg acccatacaa gtattggaag atcttcttca gaaatcaact tttgttccat
1021 ggatccccct cgagtatcgt tcgtaaatgg tgaaaatttt cagaaaattg cttttgcttt
1081 aaaagaaatg atttaaattg ctgcaataga agtagaatgc ttgattgctt gagattcgtt
1141 tgttttgtat atgttgtgtt gaggtcgagg tcctctccaa atgaaatgaa cttccttata
1201 tagaggaagg gtcttgcgaa ggatagtggg attgtgcgtc atcccttacg tcagtggaga
1261 tatcacatca atccacttgc tttgaagacg tggttggaac gtcttctttt tccacgatgc
1321 tcctcgtggg tgggggtcca tctttgggac cactgtcggc agaggcatct tcaacgatgg
1381 cctttccttt atcgcaatga tggcatttgt aggagccacc ttccttttcc actatcttca
1441 caataaagtg acagatagct gggcaatgga atccgaggag gtttccggat attacccttt
1501 gttgaaaagt ctcaattgcc ctttggtctt ctgagactgt atctttgata tttttggagt
1561 agacaagtgt gtcgtgctcc accatgttat cacatcaatc cacttgcttt gaagacgtgg
1621 ttggaacgtc ttctttttcc acgatgctcc tcgtgggtgg gggtccatct ttgggaccac
1681 tgtcggcaga ggcatcttca acgatggcct ttcctttatc gcaatgatgg catttgtagg
1741 agccaccttc cttttccact atcttcacaa taaagtgaca gatagctggg caatggaatc
1801 cgaggaggtt tccggatatt accctttgtt gaaaagtctc aattgccctt tggtcttctg
1861 agactgtatc tttgatattt ttggagtaga caagtgtgtc gtgctccacc atgttgacct
1921 gcaggcatgc aagcttggca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
1981 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
2041 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgctaga
2101 gcagcttgag cttggatcag attgtcgttt cccgccttca gtttaaacta tcagtgtttg
2161 acaggatata ttggcgggta aacctaagag aaaagagcgt ttattagaat aacggatatt
2221 taaaagggcg tgaaaaggtt tatccgttcg tccatttgta tgtgcatgcc aaccacaggg
2281 ttcccctcgg gatcaaagta ctttgatcca acccctccgc tgctatagtg cagtcggctt
2341 ctgacgttca gtgcagccgt cttctgaaaa cgacatgtcg cacaagtcct aagttacgcg
2401 acaggctgcc gccctgccct tttcctggcg ttttcttgtc gcgtgtttta gtcgcataaa
2461 gtagaatact tgcgactaga accggagaca ttacgccatg aacaagagcg ccgccgctgg
2521 cctgctgggc tatgcccgcg tcagcaccga cgaccaggac ttgaccaacc aacgggccga
2581 actgcacgcg gccggctgca ccaagctgtt ttccgagaag atcaccggca ccaggcgcga
2641 ccgcccggag ctggccagga tgcttgacca cctagccctg gcgacgttgt gacagtgacc
2701 aggctagacc gcctggcccg cagcacccgc gacctactgg acattgccga gcgcatccag
2761 gaggccggcg cgggcctgcg tagcctggca gagccgtggg ccgacaccac cacgccggcc
2821 ggccgcatgg tgttgaccgt gttcgccggc attgccgagt tcgagcgttc cctaatcatc
2881 gaccgcaccc ggagcgggcg cgaggccgcc aaggcccgag gcgtgaagtt tggcccccgc
2941 cctaccctca ccccggcaca gatcgcgcac gcccgcgagc tgatcgacca ggaaggccgc
3001 accgtgaaag aggcggctgc actgcttggc gtgcatcgct cgaccctgta ccgcgcactt
3061 gagcgcagcg aggaagtgac gcccaccgag gccaggcggc gcggtgcctt ccgtgaggac
3121 gcattgaccg aggccgacgc cctggcggcc gccgagaatg aacgccaaga ggaacaagca
3181 tgaaaccgca ccaggacggc caggacgaac cgtttttcat taccgaagag atcgaggcgg
3241 agatgatcgc ggccgggtac gtgttcgagc cgcccgcgca cgtctcaacc gtgcggctgc
3301 atgaaatcct ggccggtttg tctgatgcca agctggcggc ctggccggcc agcttggccg
3361 ctgaagaaac cgagcgccgc cgtctaaaaa ggtgatgtgt atttgagtaa aacagcttgc
3421 gtcatgcggt cgctgcgtat atgatgcgat gagtaaataa acaaatacgc aaggggaacg
3481 catgaaggtt atcgctgtac ttaaccagaa aggcgggtca ggcaagacga ccatcgcaac
3541 ccatctagcc cgcgccctgc aactcgccgg ggccgatgtt ctgttagtcg attccgatcc
3601 ccagggcagt gcccgcgatt gggcggccgt gcgggaagat caaccgctaa ccgttgtcgg
3661 catcgaccgc ccgacgattg accgcgacgt gaaggccatc ggccggcgcg acttcgtagt
3721 gatcgacgga gcgccccagg cggcggactt ggctgtgtcc gcgatcaagg cagccgactt
3781 cgtgctgatt ccggtgcagc caagccctta cgacatatgg gccaccgccg acctggtgga
3841 gctggttaag cagcgcattg aggtcacgga tggaaggcta caagcggcct ttgtcgtgtc
3901 gcgggcgatc aaaggcacgc gcatcggcgg tgaggttgcc gaggcgctgg ccgggtacga
3961 gctgcccatt cttgagtccc gtatcacgca gcgcgtgagc tacccaggca ctgccgccgc
4021 cggcacaacc gttcttgaat cagaacccga gggcgacgct gcccgcgagg tccaggcgct
4081 ggccgctgaa attaaatcaa aactcatttg agttaatgag gtaaagagaa aatgagcaaa
4141 agcacaaaca cgctaagtgc cggccgtccg agcgcacgca gcagcaaggc tgcaacgttg
4201 gccagcctgg cagacacgcc agccatgaag cgggtcaact ttcagttgcc ggcggaggat
4261 cacaccaagc tgaagatgta cgcggtacgc caaggcaaga ccattaccga gctgctatct
4321 gaatacatcg cgcagctacc agagtaaatg agcaaatgaa taaatgagta gatgaatttt
4381 agcggctaaa ggaggcggca tggaaaatca agaacaacca ggcaccgacg ccgtggaatg
4441 ccccatgtgt ggaggaacgg gcggttggcc aggcgtaagc ggctgggttg tctgccggcc
4501 ctgcaatggc actggaaccc ccaagcccga ggaatcggcg tgacggtcgc aaaccatccg
4561 gcccggtaca aatcggcgcg gcgctgggtg atgacctggt ggagaagttg aaggccgcgc
4621 aggccgccca gcggcaacgc atcgaggcag aagcacgccc cggtgaatcg tggcaagcgg
4681 ccgctgatcg aatccgcaaa gaatcccggc aaccgccggc agccggtgcg ccgtcgatta
4741 ggaagccgcc caagggcgac gagcaaccag attttttcgt tccgatgctc tatgacgtgg
4801 gcacccgcga tagtcgcagc atcatggacg tggccgtttt ccgtctgtcg aagcgtgacc
4861 gacgagctgg cgaggtgatc cgctacgagc ttccagacgg gcacgtagag gtttccgcag
4921 ggccggccgg catggccagt gtgtgggatt acgacctggt actgatggcg gtttcccatc
4981 taaccgaatc catgaaccga taccgggaag ggaagggaga caagcccggc cgcgtgttcc
5041 gtccacacgt tgcggacgta ctcaagttct gccggcgagc cgatggcgga aagcagaaag
5101 acgacctggt agaaacctgc attcggttaa acaccacgca cgttgccatg cagcgtacga
5161 agaaggccaa gaacggccgc ctggtgacgg tatccgaggg tgaagccttg attagccgct
5221 acaagatcgt aaagagcgaa accgggcggc cggagtacat cgagatcgag ctagctgatt
5281 ggatgtaccg cgagatcaca gaaggcaaga acccggacgt gctgacggtt caccccgatt
5341 actttttgat cgatcccggc atcggccgtt ttctctaccg cctggcacgc cgcgccgcag
5401 gcaaggcaga agccagatgg ttgttcaaga cgatctacga acgcagtggc agcgccggag
5461 agttcaagaa gttctgtttc accgtgcgca agctgatcgg gtcaaatgac ctgccggagt
5521 acgatttgaa ggaggaggcg gggcaggctg gcccgatcct agtcatgcgc taccgcaacc
5581 tgatcgaggg cgaagcatcc gccggttcct aatgtacgga gcagatgcta gggcaaattg
5641 ccctagcagg ggaaaaaggt cgaaaaggtc tctttcctgt ggatagcacg tacattggga
5701 acccaaagcc gtacattggg aaccggaacc cgtacattgg gaacccaaag ccgtacattg
5761 ggaaccggtc acacatgtaa gtgactgata taaaagagaa aaaaggcgat ttttccgcct
5821 aaaactcttt aaaacttatt aaaactctta aaacccgcct ggcctgtgca taactgtctg
5881 gccagcgcac agccgaagag ctgcaaaaag cgcctaccct tcggtcgctg cgctccctac
5941 gccccgccgc ttcgcgtcgg cctatcgcgg ccgctggccg ctcaaaaatg gctggcctac
6001 ggccaggcaa tctaccaggg cgcggacaag ccgcgccgtc gccactcgac cgccggcgcc
6061 cacatcaagg caccctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg
6121 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt
6181 cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc
6241 gatagcggag tgtatactgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
6301 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgct
6361 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
6421 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
6481 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
6541 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
6601 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
6661 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
6721 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
6781 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
6841 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
6901 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
6961 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
7021 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
7081 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
7141 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
7201 tcatgcattc taggtactaa aacaattcat ccagtaaaat ataatatttt attttctccc
7261 aatcaggctt gatccccagt aagtcaaaaa atagctcgac atactgttct tccccgatat
7321 cctccctgat cgaccggacg cagaaggcaa tgtcatacca cttgtccgcc ctgccgcttc
7381 tcccaagatc aataaagcca cttactttgc catctttcac aaagatgttg ctgtctccca
7441 ggtcgccgtg ggaaaagaca agttcctctt cgggcttttc cgtctttaaa aaatcataca
7501 gctcgcgcgg atctttaaat ggagtgtctt cttcccagtt ttcgcaatcc acatcggcca
7561 gatcgttatt cagtaagtaa tccaattcgg ctaagcggct gtctaagcta ttcgtatagg
7621 gacaatccga tatgtcgatg gagtgaaaga gcctgatgca ctccgcatac agctcgataa
7681 tcttttcagg gctttgttca tcttcatact cttccgagca aaggacgcca tcggcctcac
7741 tcatgagcag attgctccag ccatcatgcc gttcaaagtg caggaccttt ggaacaggca
7801 gctttccttc cagccatagc atcatgtcct tttcccgttc cacatcatag gtggtccctt
7861 tataccggct gtccgtcatt tttaaatata ggttttcatt ttctcccacc agcttatata
7921 ccttagcagg agacattcct tccgtatctt ttacgcagcg gtatttttcg atcagttttt
7981 tcaattccgg tgatattctc attttagcca tttattattt ccttcctctt ttctacagta
8041 tttaaagata ccccaagaag ctaattataa caagacgaac tccaattcac tgttccttgc
8101 attctaaaac cttaaatacc agaaaacagc tttttcaaag ttgttttcaa agttggcgta
8161 taacatagta tcgacggagc cgattttgaa accgcggtga tcacaggcag caacgctctg
8221 tcatcgttac aatcaacatg ctaccctccg cgagatcatc cgtgtttcaa acccggcagc
8281 ttagttgccg ttcttccgaa tagcatcggt aacatgagca aagtctgccg ccttacaacg
8341 gctctcccgc tgacgccgtc ccggactgat gggctgcctg tatcgagtgg tgattttgtg
8401 ccgagctgcc ggtcggggag ctgttggctg gctggtggca ggatatattg tggtgtaaac
8461 aaattgacgc ttagacaact taataacaca ttgcggacgt ttttaatgta ctgaattaac
8521 gccgaattaa ttcgggggat ctggatttta gtactggatt ttggttttag gaattagaaa
8581 ttttattgat agaagtattt tacaaataca aatacatact aagggtttct tatatgctca
8641 acacatgagc gaaaccctat aggaacccta attcccttat ctgggaacta ctcacacatt
8701 attatggaga aactcgagct tgtcgatcga cagatccggt cggcatctac tctatttctt
8761 tgccctcgga cgagtgctgg ggcgtcggtt tccactatcg gcgagtactt ctacacagcc
8821 atcggtccag acggccgcgc ttctgcgggc gatttgtgta cgcccgacag tcccggctcc
8881 ggatcggacg attgcgtcgc atcgaccctg cgcccaagct gcatcatcga aattgccgtc
8941 aaccaagctc tgatagagtt ggtcaagacc aatgcggagc atatacgccc ggagtcgtgg
9001 cgatcctgca agctccggat gcctccgctc gaagtagcgc gtctgctgct ccatacaagc
9061 caaccacggc ctccagaaga agatgttggc gacctcgtat tgggaatccc cgaacatcgc
9121 ctcgctccag tcaatgaccg ctgttatgcg gccattgtcc gtcaggacat tgttggagcc
9181 gaaatccgcg tgcacgaggt gccggacttc ggggcagtcc tcggcccaaa gcatcagctc
9241 atcgagagcc tgcgcgacgg acgcactgac ggtgtcgtcc atcacagttt gccagtgata
9301 cacatgggga tcagcaatcg cgcatatgaa atcacgccat gtagtgtatt gaccgattcc
9361 ttgcggtccg aatgggccga acccgctcgt ctggctaaga tcggccgcag cgatcgcatc
9421 catagcctcc gcgaccggtt gtagaacagc gggcagttcg gtttcaggca ggtcttgcaa
9481 cgtgacaccc tgtgcacggc gggagatgca ataggtcagg ctctcgctaa actccccaat
9541 gtcaagcact tccggaatcg ggagcgcggc cgatgcaaag tgccgataaa cataacgatc
9601 tttgtagaaa ccatcggcgc agctatttac ccgcaggaca tatccacgcc ctcctacatc
9661 gaagctgaaa gcacgagatt cttcgccctc cgagagctgc atcaggtcgg agacgctgtc
9721 gaacttttcg atcagaaact tctcgacaga cgtcgcggtg agttcaggct ttttcatatc
9781 tcattgcccc ccggatctgc gaaagctcga gagagataga tttgtagaga gagactggtg
9841 atttcagcgt gtcctctcca aatgaaatga acttccttat atagaggaag gtcttgcgaa
9901 ggatagtggg attgtgcgtc atcccttacg tcagtggaga tatcacatca atccacttgc
9961 tttgaagacg tggttggaac gtcttctttt tccacgatgc tcctcgtggg tgggggtcca
10021 tctttgggac cactgtcggc agaggcatct tgaacgatag cctttccttt atcgcaatga
10081 tggcatttgt aggtgccacc ttccttttct actgtccttt tgatgaagtg acagatagct
10141 gggcaatgga atccgaggag gtttcccgat attacccttt gttgaaaagt ctcaatagcc
10201 ctttggtctt ctgagactgt atctttgata ttcttggagt agacgagagt gtcgtgctcc
10261 accatgttat cacatcaatc cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc
10321 acgatgctcc tcgtgggtgg gggtccatct ttgggaccac tgtcggcaga ggcatcttga
10381 acgatagcct ttcctttatc gcaatgatgg catttgtagg tgccaccttc cttttctact
10441 gtccttttga tgaagtgaca gatagctggg caatggaatc cgaggaggtt tcccgatatt
10501 accctttgtt gaaaagtctc aatagccctt tggtcttctg agactgtatc tttgatattc
10561 ttggagtaga cgagagtgtc gtgctccacc atgttggcaa gctgctctag ccaatacgca
10621 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
10681 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
10741 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
10801 aatttcacac aggaaacagc tatgaccatg attac
//