LOCUS Exported 13917 bp ds-DNA circular SYN 27-SEP-2017
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 13917)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Sep 27, 2017 from MiaoLingPlasmid
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..13917
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS complement(145..516)
/codon_start=1
/gene="traJ"
/product="oriT-recognizing protein"
/label=traJ
/translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSLSAYLLAV
GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
KQDELGKVMMGVVRPRAEP"
oriT 549..658
/note="incP origin of transfer"
CDS complement(1339..1989)
/codon_start=1
/gene="tetR"
/product="tetracycline resistance regulatory protein"
/label=TetR
/translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
misc_feature 2454..2478
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
promoter 2634..2817
/function="NOS promoter"
/label=NOS promoter
/note="nopaline synthase promoter"
CDS 2838..3632
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=neo
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
terminator 4022..4274
/label=NOS terminator
/note="nopaline synthase terminator and poly(A) signal"
misc_feature 4950..4990
/label=MCS
CDS 5004..6815
/codon_start=1
/gene="uidA (or gusA) from E. coli"
/product="beta-glucuronidase"
/label=GUS
/translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAI
AVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEV
MEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHD
FFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQ
VVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGEQF
LINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDW
ADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARD
KNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISD
LFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMY
TDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRK
PKSAAFLLQKRWTGMNFGEKPQQGGKQ"
terminator 6886..7138
/label=NOS terminator
/note="nopaline synthase terminator and poly(A) signal"
misc_feature 7781..7805
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA"
CDS complement(9401..10549)
/codon_start=1
/product="trans-acting replication protein that binds to
and activates oriV"
/label=trfA
/translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
CDS complement(10848..11642)
/codon_start=1
/gene="aphA-3"
/product="aminoglycoside phosphotransferase"
/label=KanR
/note="confers resistance to kanamycin"
/translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
mobile_element 11834..12601
/mobile_element_type="insertion sequence:IS1"
/note="prokaryotic transposable element"
rep_origin 13224..18
/label=oriV
/note="incP origin of replication"
ORIGIN
1 tgagcgtcgc aaaggcgctc ggtcttgcct tgctcgtcgg tgatgtactt caccagctcc
61 gcgaagtcgc tcttcttgat ggagcgcatg gggacgtgct tggcaatcac gcgcaccccc
121 cggccgtttt agcggctaaa aaagtcatgg ctctgccctc gggcggacca cgcccatcat
181 gaccttgcca agctcgtcct gcttctcttc gatcttcgcc agcagggcga ggatcgtggc
241 atcaccgaac cgcgccgtgc gcgggtcgtc ggtgagccag agtttcagca ggccgcccag
301 gcggcccagg tcgccattga tgcgggccag ctcgcggacg tgctcatagt ccacgacgcc
361 cgtgattttg tagccctggc cgacggccag caggtaggcc gacaggctca tgccggccgc
421 cgccgccttt tcctcaatcg ctcttcgttc gtctggaagg cagtacacct tgataggtgg
481 gctgcccttc ctggttggct tggtttcatc agccatccgc ttgccctcat ctgttacgcc
541 ggcggtagcc ggccagcctc gcagagcagg attcccgttg agcaccgcca ggtgcgaata
601 agggacagtg aagaaggaac acccgctcgc gggtgggcct acttcaccta tcctgcccgg
661 ctgacgccgt tggatacacc aaggaaagtc tacacgaacc ctttggcaaa atcctgtata
721 tcgtgcgaaa aaggatggat ataccgaaaa aatcgctata atgaccccga agcagggtta
781 tgcagcggaa aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
841 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
901 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
961 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
1021 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
1081 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
1141 cagtgagcga ggaagcggaa gagcgccaga aggccgccag agaggccgag cgcggccgtg
1201 aggcttggac gctagggcag ggcatgaaaa agcccgtagc gggctgctac gggcgtctga
1261 cgcggtggaa agggggaggg gatgttgtct acatggctct gctgtagtga gtgggttgcg
1321 ctccggcagc ggtcctgatc aatcgtcacc ctttctcggt ccttcaacgt tcctgacaac
1381 gagcctcctt ttcgccaatc catcgacaat caccgcgagt ccctgctcga acgctgcgtc
1441 cggaccggct tcgtcgaagg cgtctatcgc ggcccgcaac agcggcgaga gcggagcctg
1501 ttcaacggtg ccgccgcgct cgccggcatc gctgtcgccg gcctgctcct caagcacggc
1561 cccaacagtg aagtagctga ttgtcatcag cgcattgacg gcgtccccgg ccgaaaaacc
1621 cgcctcgcag aggaagcgaa gctgcgcgtc ggccgtttcc atctgcggtg cgcccggtcg
1681 cgtgccggca tggatgcgcg cgccatcgcg gtaggcgagc agcgcctgcc tgaagctgcg
1741 ggcattcccg atcagaaatg agcgccagtc gtcgtcggct ctcggcaccg aatgcgtatg
1801 attctccgcc agcatggctt cggccagtgc gtcgagcagc gcccgcttgt tcctgaagtg
1861 ccagtaaagc gccggctgct gaacccccaa ccgttccgcc agtttgcgtg tcgtcagacc
1921 gtctacgccg acctcgttca acaggtccag ggcggcacgg atcactgtat tcggctgcaa
1981 ctttgtcatg cttgacactt tatcactgat aaacataata tgtccaccaa cttatcagtg
2041 ataaagaatc cgcgcgttca atcggaccag cggaggctgg tccggaggcc agacgtgaaa
2101 cccaacatac ccctgatcgt aattctgagc actgtcgcgc tcgacgctgt cggcatcggc
2161 ctgattatgc cggtgctgcc gggcctcctg cgcgatctgg ttcactcgaa cgacgtcacc
2221 gcccactatg gcattctgct ggcgctgtat gcgttggtgc aatttgcctg cgcacctgtg
2281 ctgggcgcgc tgtcggatcg tttcgggcgg cggccaatct tgctcgtctc gctggccggc
2341 gccagatctg gggaaccctg tggttggcat gcacatacaa atggacgaac ggataaacct
2401 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
2461 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
2521 tcatgagcgg agaattaagg gagtcacgtt atgacccccg ccgatgacgc gggacaagcc
2581 gttttacgtt tggaactgac agaaccgcaa cgttgaagga gccactcagc cgcgggtttc
2641 tggagtttaa tgagctaagc acatacgtca gaaaccatta ttgcgcgttc aaaagtcgcc
2701 taaggtcact atcagctagc aaatatttct tgtcaaaaat gctccactga cgttccataa
2761 attcccctcg gtatccaatt agagtctcat attcactctc aatccaaata atctgcaccg
2821 gatctggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
2881 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
2941 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
3001 gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg
3061 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
3121 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
3181 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
3241 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
3301 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
3361 aggcgcgcat gcccgacggc gatgatctcg tcgtgaccca tggcgatgcc tgcttgccga
3421 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
3481 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
3541 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
3601 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga
3661 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
3721 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
3781 catgctggag ttcttcgccc acgggatctc tgcggaacag gcggtcgaag gtgccgatat
3841 cattacgaca gcaacggccg acaagcacaa cgccacgatc ctgagcgaca atatgatcgg
3901 gcccggcgtc cacatcaacg gcgtcggcgg cgactgccca ggcaagaccg agatgcaccg
3961 cgatatcttg ctgcgttcgg atattttcgt ggagttcccg ccacagaccc ggatgatccc
4021 cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc
4081 gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaatg
4141 catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata
4201 cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc
4261 tatgttacta gatcgggcct cctgtcaatg ctggcggcgg ctctggtggt ggttctggtg
4321 gcggctctga gggtggtggc tctgagggtg gcggttctga gggtggcggc tctgagggag
4381 gcggttccgg tggtggctct ggttccggtg attttgatta tgaaaagatg gcaaacgcta
4441 ataagggggc tatgaccgaa aatgccgatg aaaacgcgct acagtctgac gctaaaggca
4501 aacttgattc tgtcgctact gattacggtg ctgctatcga tggtttcatt ggtgacgttt
4561 ccggccttgc taatggtaat ggtgctactg gtgattttgc tggctctaat tcccaaatgg
4621 ctcaagtcgg tgacggtgat aattcacctt taatgaataa tttccgtcaa tatttacctt
4681 ccctccctca atcggttgaa tgtcgccctt ttgtctttgg cccaatacgc aaaccgcctc
4741 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
4801 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
4861 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
4921 caggaaacag ctatgaccat gattacgcca agcttgcatg cctgcaggtc gactctagag
4981 gatccccggg tggtcagtcc cttatgttac gtcctgtaga aaccccaacc cgtgaaatca
5041 aaaaactcga cggcctgtgg gcattcagtc tggatcgcga aaactgtgga attgatcagc
5101 gttggtggga aagcgcgtta caagaaagcc gggcaattgc tgtgccaggc agttttaacg
5161 atcagttcgc cgatgcagat attcgtaatt atgcgggcaa cgtctggtat cagcgcgaag
5221 tctttatacc gaaaggttgg gcaggccagc gtatcgtgct gcgtttcgat gcggtcactc
5281 attacggcaa agtgtgggtc aataatcagg aagtgatgga gcatcagggc ggctatacgc
5341 catttgaagc cgatgtcacg ccgtatgtta ttgccgggaa aagtgtacgt atcaccgttt
5401 gtgtgaacaa cgaactgaac tggcagacta tcccgccggg aatggtgatt accgacgaaa
5461 acggcaagaa aaagcagtct tacttccatg atttctttaa ctatgccgga atccatcgca
5521 gcgtaatgct ctacaccacg ccgaacacct gggtggacga tatcaccgtg gtgacgcatg
5581 tcgcgcaaga ctgtaaccac gcgtctgttg actggcaggt ggtggccaat ggtgatgtca
5641 gcgttgaact gcgtgatgcg gatcaacagg tggttgcaac tggacaaggc actagcggga
5701 ctttgcaagt ggtgaatccg cacctctggc aaccgggtga aggttatctc tatgaactgt
5761 gcgtcacagc caaaagccag acagagtgtg atatctaccc gcttcgcgtc ggcatccggt
5821 cagtggcagt gaagggcgaa cagttcctga ttaaccacaa accgttctac tttactggct
5881 ttggtcgtca tgaagatgcg gacttgcgtg gcaaaggatt cgataacgtg ctgatggtgc
5941 acgaccacgc attaatggac tggattgggg ccaactccta ccgtacctcg cattaccctt
6001 acgctgaaga gatgctcgac tgggcagatg aacatggcat cgtggtgatt gatgaaactg
6061 ctgctgtcgg ctttaacctc tctttaggca ttggtttcga agcgggcaac aagccgaaag
6121 aactgtacag cgaagaggca gtcaacgggg aaactcagca agcgcactta caggcgatta
6181 aagagctgat agcgcgtgac aaaaaccacc caagcgtggt gatgtggagt attgccaacg
6241 aaccggatac ccgtccgcaa ggtgcacggg aatatttcgc gccactggcg gaagcaacgc
6301 gtaaactcga cccgacgcgt ccgatcacct gcgtcaatgt aatgttctgc gacgctcaca
6361 ccgataccat cagcgatctc tttgatgtgc tgtgcctgaa ccgttattac ggatggtatg
6421 tccaaagcgg cgatttggaa acggcagaga aggtactgga aaaagaactt ctggcctggc
6481 aggagaaact gcatcagccg attatcatca ccgaatacgg cgtggatacg ttagccgggc
6541 tgcactcaat gtacaccgac atgtggagtg aagagtatca gtgtgcatgg ctggatatgt
6601 atcaccgcgt ctttgatcgc gtcagcgccg tcgtcggtga acaggtatgg aatttcgccg
6661 attttgcgac ctcgcaaggc atattgcgcg ttggcggtaa caagaaaggg atcttcactc
6721 gcgaccgcaa accgaagtcg gcggcttttc tgctgcaaaa acgctggact ggcatgaact
6781 tcggtgaaaa accgcagcag ggaggcaaac aatgaatcaa caactctcct ggcgcaccat
6841 cgtcggctac agcctcggga attgctaccg agctcgaatt tccccgatcg ttcaaacatt
6901 tggcaataaa gtttcttaag attgaatcct gttgccggtc ttgcgatgat tatcatataa
6961 tttctgttga attacgttaa gcatgtaata attaacatgt aatgcatgac gttatttatg
7021 agatgggttt ttatgattag agtcccgcaa ttatacattt aatacgcgat agaaaacaaa
7081 atatagcgcg caaactagga taaattatcg cgcgcggtgt catctatgtt actagatcgg
7141 gaattcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
7201 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
7261 cgatcgccct tcccaacagt tgcgcagcct gaatggcgcc cgctcctttc gctttcttcc
7321 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
7381 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg
7441 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
7501 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcgggct
7561 attcttttga tttataaggg attttgccga tttcggaacc accatcaaac aggattttcg
7621 cctgctgggg caaaccagcg tggaccgctt gctgcaactc tctcagggcc aggcggtgaa
7681 gggcaatcag ctgttgcccg tctcactggt gaaaagaaaa accaccccag tacattaaaa
7741 acgtccgcaa tgtgttatta agttgtctaa gcgtcaattt gtttacacca caatatatcc
7801 tgccaccagc cagccaacag ctccccgacc ggcagctcgg cacaaaatca ccactcgata
7861 caggcagccc atcagtccgg gacggcgtca gcgggagagc cgttgtaagg cggcagactt
7921 tgctcatgtt accgatgcta ttcggaagaa cggcaactaa gctgccgggt ttgaaacacg
7981 gatgatctcg cggagggtag catgttgatt gtaacgatga cagagcgttg ctgcctgtga
8041 tcaaatatca tctccctcgc agagatccga attatcagcc ttcttattca tttctcgctt
8101 aaccgtgaca ggctgtcgat cttgagaact atgccgacat aataggaaat cgctggataa
8161 agccgctgag gaagctgagt ggcgctattt ctttagaagt gaacgttgac gatatcaact
8221 cccctatcca ttgctcaccg aatggtacag gtcggggacc cgaagttccg actgtcggcc
8281 tgatgcatcc ccggctgatc gaccccagat ctggggctga gaaagcccag taaggaaaca
8341 actgtaggtt cgagtcgcga gatcccccgg aaccaaagga agtaggttaa acccgctccg
8401 atcaggccga gccacgccag gccgagaaca ttggttcctg taggcatcgg gattggcgga
8461 tcaaacacta aagctactgg aacgagcaga agtcctccgg ccgccagttg ccaggcggta
8521 aaggtgagca gaggcacggg aggttgccac ttgcgggtca gcacggttcc gaacgccatg
8581 gaaaccgccc ccgccaggcc cgctgcgacg ccgacaggat ctagcgctgc gtttggtgtc
8641 aacaccaaca gcgccacgcc cgcagttccg caaatagccc ccaggaccgc catcaatcgt
8701 atcgggctac ctagcagagc ggcagagatg aacacgacca tcagcggctg cacagcgcct
8761 accgtcgccg cgaccccgcc cggcaggcgg tagaccgaaa taaacaacaa gctccagaat
8821 agcgaaatat taagtgcgcc gaggatgaag atgcgcatcc accagattcc cgttggaatc
8881 tgtcggacga tcatcacgag caataaaccc gccggcaacg cccgcagcag cataccggcg
8941 acccctcggc ctcgctgttc gggctccacg aaaacgccgg acagatgcgc cttgtgagcg
9001 tccttggggc cgtcctcctg tttgaagacc gacagcccaa tgatctcgcc gtcgatgtag
9061 gcgccgaatg ccacggcatc tcgcaaccgt tcagcgaacg cctccatggg ctttttctcc
9121 tcgtgctcgt aaacggaccc gaacatctct ggagctttct tcagggccga caatcggatc
9181 tcgcggaaat cctgcacgtc ggccgctcca agccgtcgaa tctgagcctt aatcacaatt
9241 gtcaatttta atcctctgtt tatcggcagt tcgtagagcg cgccgtgcgt cccgagcgat
9301 actgagcgaa gcaagtgcgt cgagcagtgc ccgcttgttc ctgaaatgcc agtaaagcgc
9361 tggctgctga acccccagcc ggaactgacc ccacaaggcc ctagcgtttg caatgcacca
9421 ggtcatcatt gacccaggcg tgttccacca ggccgctgcc tcgcaactct tcgcaggctt
9481 cgccgacctg ctcgcgccac ttcttcacgc gggtggaatc cgatccgcac atgaggcgga
9541 aggtttccag cttgagcggg tacggctccc ggtgcgagct gaaatagtcg aacatccgtc
9601 gggccgtcgg cgacagcttg cggtacttct cccatatgaa tttcgtgtag tggtcgccag
9661 caaacagcac gacgatttcc tcgtcgatca ggacctggca acgggacgtt ttcttgccac
9721 ggtccaggac gcggaagcgg tgcagcagcg acaccgattc caggtgccca acgcggtcgg
9781 acgtgaagcc catcgccgtc gcctgtaggc gcgacaggca ttcctcggcc ttcgtgtaat
9841 accggccatt gatcgaccag cccaggtcct ggcaaagctc gtagaacgtg aaggtgatcg
9901 gctcgccgat aggggtgcgc ttcgcgtact ccaacacctg ctgccacacc agttcgtcat
9961 cgtcggcccg cagctcgacg ccggtgtagg tgatcttcac gtccttgttg acgtggaaaa
10021 tgaccttgtt ttgcagcgcc tcgcgcggga ttttcttgtt gcgcgtggtg aacagggcag
10081 agcgggccgt gtcgtttggc atcgctcgca tcgtgtccgg ccacggcgca atatcgaaca
10141 aggaaagctg catttccttg atctgctgct tcgtgtgttt cagcaacgcg gcctgcttgg
10201 cctcgctgac ctgttttgcc aggtcctcgc cggcggtttt tcgcttcttg gtcgtcatag
10261 ttcctcgcgt gtcgatggtc atcgacttcg ccaaacctgc cgcctcctgt tcgagacgac
10321 gcgaacgctc cacggcggcc gatggcgcgg gcagggcagg gggagccagt tgcacgctgt
10381 cgcgctcgat cttggccgta gcttgctgga ccatcgagcc gacggactgg aaggtttcgc
10441 ggggcgcacg catgacggtg cggcttgcga tggtttcggc atcctcggcg gaaaaccccg
10501 cgtcgatcag ttcttgcctg tatgccttcc ggtcaaacgt ccgattcatt caccctcctt
10561 gcgggattgc cccgactcac gccggggcaa tgtgccctta ttcctgattt gacccgcctg
10621 gtgccttggt gtccagataa tccaccttat cggcaatgaa gtcggtcccg tagaccgtct
10681 ggccgtcctt ctcgtacttg gtattccgaa tcttgccctg cacgaatacc agcgacccct
10741 tgcccaaata cttgccgtgg gcctcggcct gagagccaaa acacttgatg cggaagaagt
10801 cggtgcgctc ctgcttgtcg ccggcatcgt tgcgccacat ctaggtacta aaacaattca
10861 tccagtaaaa tataatattt tattttctcc caatcaggct tgatccccag taagtcaaaa
10921 aatagctcga catactgttc ttccccgata tcctccctga tcgaccggac gcagaaggca
10981 atgtcatacc acttgtccgc cctgccgctt ctcccaagat caataaagcc acttactttg
11041 ccatctttca caaagatgtt gctgtctccc aggtcgccgt gggaaaagac aagttcctct
11101 tcgggctttt ccgtctttaa aaaatcatac agctcgcgcg gatctttaaa tggagtgtct
11161 tcttcccagt tttcgcaatc cacatcggcc agatcgttat tcagtaagta atccaattcg
11221 gctaagcggc tgtctaagct attcgtatag ggacaatccg atatgtcgat ggagtgaaag
11281 agcctgatgc actccgcata cagctcgata atcttttcag ggctttgttc atcttcatac
11341 tcttccgagc aaaggacgcc atcggcctca ctcatgagca gattgctcca gccatcatgc
11401 cgttcaaagt gcaggacctt tggaacaggc agctttcctt ccagccatag catcatgtcc
11461 ttttcccgtt ccacatcata ggtggtccct ttataccggc tgtccgtcat ttttaaatat
11521 aggttttcat tttctcccac cagcttatat accttagcag gagacattcc ttccgtatct
11581 tttacgcagc ggtatttttc gatcagtttt ttcaattccg gtgatattct cattttagcc
11641 atttattatt tccttcctct tttctacagt atttaaagat accccaagaa gctaattata
11701 acaagacgaa ctccaattca ctgttccttg cattctaaaa ccttaaatac cagaaaacag
11761 ctttttcaaa gttgttttca aagttggcgt ataacatagt atcgacggag ccgattttga
11821 aaccacaatt atgggtgatg ctgccaactt actgatttag tgtatgatgg tgtttttgag
11881 gtgctccagt ggcttctgtg tctatcagct gtccctcctg ttcagctact gacggggtgg
11941 tgcgtaacgg caaaagcacc gccggacatc agcgctatct ctgctctcac tgccgtaaaa
12001 catggcaact gcagttcact tacaccgctt ctcaacccgg tacgcaccag aaaatcattg
12061 atatggccat gaatggcgtt ggatgccggg caacagcccg cattatgggc gttggcctca
12121 acacgatttt acgtcactta aaaaactcag gccgcagtcg gtaacctcgc gcatacagcc
12181 gggcagtgac gtcatcgtct gcgcggaaat ggacgaacag tggggctatg tcggggctaa
12241 atcgcgccag cgctggctgt tttacgcgta tgacagtctc cggaagacgg ttgttgcgca
12301 cgtattcggt gaacgcacta tggcgacgct ggggcgtctt atgagcctgc tgtcaccctt
12361 tgacgtggtg atatggatga cggatggctg gccgctgtat gaatcccgcc tgaagggaaa
12421 gctgcacgta atcagcaagc gatatacgca gcgaattgag cggcataacc tgaatctgag
12481 gcagcacctg gcacggctgg gacggaagtc gctgtcgttc tcaaaatcgg tggagctgca
12541 tgacaaagtc atcgggcatt atctgaacat aaaacactat caataagttg gagtcattac
12601 ccaattatga tagaatttac aagctataag gttattgtcc tgggtttcaa gcattagtcc
12661 atgcaagttt ttatgctttg cccattctat agatatattg ataagcgcgc tgcctatgcc
12721 ttgccccctg aaatccttac atacggcgat atcttctata taaaagatat attatcttat
12781 cagtattgtc aatatattca aggcaatctg cctcctcatc ctcttcatcc tcttcgtctt
12841 ggtagctttt taaatatggc gcttcataga gtaattctgt aaaggtccaa ttctcgtttt
12901 catacctcgg tataatctta cctatcacct caaatggttc gctgggttta tcgcaccccc
12961 gaacacgagc acggcacccg cgaccactat gccaagaatg cccaaggtaa aaattgccgg
13021 ccccgccatg aagtccgtga atgccccgac ggccgaagtg aagggcaggc cgccacccag
13081 gccgccgccc tcactgcccg gcacctggtc gctgaatgtc gatgccagca cctgcggcac
13141 gtcaatgctt ccgggcgtcg cgctcgggct gatcgcccat cccgttactg ccccgatccc
13201 ggcaatggca aggactgcca gcgctgccat ttttggggtg aggccgttcg cggccgaggg
13261 gcgcagcccc tggggggatg ggaggcccgc gttagcgggc cgggagggtt cgagaagggg
13321 gggcaccccc cttcggcgtg cgcggtcacg cgcacagggc gcagccctgg ttaaaaacaa
13381 ggtttataaa tattggttta aaagcaggtt aaaagacagg ttagcggtgg ccgaaaaacg
13441 ggcggaaacc cttgcaaatg ctggattttc tgcctgtgga cagcccctca aatgtcaata
13501 ggtgcgcccc tcatctgtca gcactctgcc cctcaagtgt caaggatcgc gcccctcatc
13561 tgtcagtagt cgcgcccctc aagtgtcaat accgcagggc acttatcccc aggcttgtcc
13621 acatcatctg tgggaaactc gcgtaaaatc aggcgttttc gccgatttgc gaggctggcc
13681 agctccacgt cgccggccga aatcgagcct gcccctcatc tgtcaacgcc gcgccgggtg
13741 agtcggcccc tcaagtgtca acgtccgccc ctcatctgtc agtgagggcc aagttttccg
13801 cgaggtatcc acaacgccgg cggccgcggt gtctcgcaca cggcttcgac ggcgtttctg
13861 gcgcgtttgc agggccatag acggccgcca gcccagcggc gagggcaacc agcccgg
//