LOCUS Exported 10531 bp ds-DNA circular SYN 17-SEP-2017
DEFINITION synthetic circular DNA
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10531)
TITLE Direct Submission
JOURNAL Exported Sep 17, 2017 from MiaoLingPlasmid
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..10531
/organism="synthetic DNA construct"
/mol_type="other DNA"
rep_origin 1129..1323
/label=pVS1 oriV
/note="origin of replication for the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
misc_feature 1667..1807
/label=bom
/note="basis of mobility region from pBR322"
rep_origin complement(1993..2581)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(2668..3462)
/codon_start=1
/gene="aphA-3"
/product="aminoglycoside phosphotransferase"
/label=KanR
/note="confers resistance to kanamycin"
/translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
misc_feature 3887..3911
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA"
polyA_signal 3989..4163
/label=CaMV poly(A) signal
/note="cauliflower mosaic virus polyadenylation signal"
CDS complement(4189..5331)
/codon_start=1
/label=Hyg
/translation="MTHNPTILRKTFLYIRKFISFGEDTLKSPVSLYKSISPRMKKPEL
TATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYVLRVNSCADGFYKDRYVYR
HFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPETELPAVLQPVAEAMDAIAA
ADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTVMDDTVSASVAQALDELML
WAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGDSQYEVANIFFWRPWLACM
EQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNFDDAAWAQGRCDAIVRSGA
GTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPGSWR"
promoter complement(5253..5930)
/label=CaMV 35S promoter (enhanced)
/note="cauliflower mosaic virus 35S promoter with a
duplicated enhancer region"
misc_feature 8140..8164
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
CDS 9464..10093
/codon_start=1
/product="stability protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/label=pVS1 StaA
/translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
1 caaaccatcc ggcccggtac aaatcggcgc ggcgctgggt gatgacctgg tggagaagtt
61 gaaggccgcg caggccgccc agcggcaacg catcgaggca gaagcacgcc ccggtgaatc
121 gtggcaagcg gccgctgatc gaatccgcaa agaatcccgg caaccgccgg cagccggtgc
181 gccgtcgatt aggaagccgc ccaagggcga cgagcaacca gattttttcg ttccgatgct
241 ctatgacgtg ggcacccgcg atagtcgcag catcatggac gtggccgttt tccgtctgtc
301 gaagcgtgac cgacgagctg gcgaggtgat ccgctacgag cttccagacg ggcacgtaga
361 ggtttccgca gggccggccg gcatggccag tgtgtgggat tacgacctgg tactgatggc
421 ggtttcccat ctaaccgaat ccatgaaccg ataccgggaa gggaagggag acaagcccgg
481 ccgcgtgttc cgtccacacg ttgcggacgt actcaagttc tgccggcgag ccgatggcgg
541 aaagcagaaa gacgacctgg tagaaacctg cattcggtta aacaccacgc acgttgccat
601 gcagcgtacg aagaaggcca agaacggccg cctggtgacg gtatccgagg gtgaagcctt
661 gattagccgc tacaagatcg taaagagcga aaccgggcgg ccggagtaca tcgagatcga
721 gctagctgat tggatgtacc gcgagatcac agaaggcaag aacccggacg tgctgacggt
781 tcaccccgat tactttttga tcgatcccgg catcggccgt tttctctacc gcctggcacg
841 ccgcgccgca ggcaaggcag aagccagatg gttgttcaag acgatctacg aacgcagtgg
901 cagcgccgga gagttcaaga agttctgttt caccgtgcgc aagctgatcg ggtcaaatga
961 cctgccggag tacgatttga aggaggaggc ggggcaggct ggcccgatcc tagtcatgcg
1021 ctaccgcaac ctgatcgagg gcgaagcatc cgccggttcc taatgtacgg agcagatgct
1081 agggcaaatt gccctagcag gggaaaaagg tcgaaaaggc ctctttcctg tggatagcac
1141 gtacattggg aacccaaagc cgtacattgg gaaccggaac ccgtacattg ggaacccaaa
1201 gccgtacatt gggaaccggt cacacatgta agtgactgat ataaaagaga aaaaaggcga
1261 tttttccgcc taaaactctt taaaacttat taaaactctt aaaacccgcc tggcctgtgc
1321 ataactgtct ggccagcgca cagccgaaga gctgcaaaaa gcgcctaccc ttcggtcgct
1381 gcgctcccta cgccccgccg cttcgcgtcg gcctatcgcg gccgctggcc gctcaaaaat
1441 ggctggccta cggccaggca atctaccagg gcgcggacaa gccgcgccgt cgccactcga
1501 ccgccggcgc ccacatcaag gcaccctgcc tcgcgcgttt cggtgatgac ggtgaaaacc
1561 tctgacacat gcagctcccg gaaacggtca cagcttgtct gtaagcggat gccgggagca
1621 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc
1681 agtcacgtag cgatagcgga gtgtatactg gcttaactat gcggcatcag agcagattgt
1741 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
1801 catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
1861 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
1921 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
1981 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
2041 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
2101 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
2161 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
2221 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
2281 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
2341 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
2401 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
2461 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
2521 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
2581 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
2641 agggattttg gtcatgcatt ctaggtacta aaacaattca tccagtaaaa tataatattt
2701 tattttctcc caatcaggct tgatccccag taagtcaaaa aatagctcga catactgttc
2761 ttccccgata tcctccctga tcgaccggac gcagaaggca atgtcatacc acttgtccgc
2821 cctgccgctt ctcccaagat caataaagcc acttactttg ccatctttca caaagatgtt
2881 gctgtctccc aggtcgccgt gggaaaagac aagttcctct tcgggctttt ccgtctttaa
2941 aaaatcatac agctcgcgcg gatctttaaa tggagtgtct tcttcccagt tttcgcaatc
3001 cacatcggcc agatcgttat tcagtaagta atccaattcg gctaagcggc tgtctaagct
3061 attcgtatag ggacaatccg atatgtcgat ggagtgaaag agcctgatgc actccgcata
3121 cagctcgata atcttttcag ggctttgttc atcttcatac tcttccgagc aaaggacgcc
3181 atcggcctca ctcatgagca gattgctcca gccatcatgc cgttcaaagt gcaggacctt
3241 tggaacaggc agctttcctt ccagccatag catcatgtcc ttttcccgtt ccacatcata
3301 ggtggtccct ttataccggc tgtccgtcat ttttaaatat aggttttcat tttctcccac
3361 cagcttatat accttagcag gagacattcc ttccgtatct tttacgcagc ggtatttttc
3421 gatcagtttt ttcaattccg gtgatattct cattttagcc atttattatt tccttcctct
3481 tttctacagt atttaaagat accccaagaa gctaattata acaagacgaa ctccaattca
3541 ctgttccttg cattctaaaa ccttaaatac cagaaaacag ctttttcaaa gttgttttca
3601 aagttggcgt ataacatagt atcgacggag ccgattttga aaccgcggtg atcacaggca
3661 gcaacgctct gtcatcgtta caatcaacat gctaccctcc gcgagatcat ccgtgtttca
3721 aacccggcag cttagttgcc gttcttccga atagcatcgg taacatgagc aaagtctgcc
3781 gccttacaac ggctctcccg ctgacgccgt cccggactga tgggctgcct gtatcgagtg
3841 gtgattttgt gccgagctgc cggtcgggga gctgttggct ggctggtggc aggatatatt
3901 gtggtgtaaa caaattgacg cttagacaac ttaataacac attgcggacg tttttaatgt
3961 actgaattaa cgccgaatta attcggggga tctggatttt agtactggat tttggtttta
4021 ggaattagaa attttattga tagaagtatt ttacaaatac aaatacatac taagggtttc
4081 ttatatgctc aacacatgag cgaaacccta taggaaccct aattccctta tctgggaact
4141 actcacacat tattatggag aaaatagagc ttgtcgatcg ttaattaatc acctccaaga
4201 tcccggacga gtgctggggc gtcggtttcc actatcggcg agtacttcta cacagccatc
4261 ggtccagacg gccgcgcttc tgcgggcgat ttgtgtacgc ccgacagtcc cggctccgga
4321 tcggacgatt gcgtcgcatc gaccctgcgc ccaagctgca tcatcgaaat tgccgtcaac
4381 caagctctga tagagttggt caagaccaat gcggagcata tacgcccgga gccgcggcga
4441 tcctgcaagc tccggatgcc tccgctcgaa gtagcgcgtc tgctgctcca tacaagccaa
4501 ccacggcctc cagaagaaga tgttggcgac ctcgtattgg gaatccccga acatcgcctc
4561 gctccagtca atgaccgctg ttatgcggcc attgtccgtc aggacattgt tggagccgaa
4621 atccgcgtgc acgaggtgcc ggacttcggg gcagtcctcg gcccaaagca tcagctcatc
4681 gagagcctgc gcgacggacg cactgacggt gtcgtccatc acagtttgcc agtgatacac
4741 atggggatca gcaatcgcgc atatgaaatc acgccatgta gtgtattgac cgattccttg
4801 cggtccgaat gggccgaacc cgctcgtctg gctaagatcg gccgcagcga tcgcatccat
4861 ggcctccgcg accggctgca gaacagcggg cagttcggtt tcaggcaggt cttgcaacgt
4921 gacaccctgt gcacggcggg agatgcaata ggtcaggctc tcgctgaatt ccccaatgtc
4981 aagcacttcc ggaatcggga gcgcggccga tgcaaagtgc cgataaacat aacgatcttt
5041 gtagaaacca tcggcgcagc tatttacccg caggacatat ccacgccctc ctacatcgaa
5101 gctgaaagca cgagattctt cgccctccga gagctgcatc aggtcggaca cgctgtcgaa
5161 cttttcgatc agaaacttct cgacagacgt cgcggtgagt tcaggctttt tcatcctagg
5221 agagatagat ttgtagagag agactggtga tttcagcgtg tcctctccaa atgaaatgaa
5281 cttccttata tagaggaagg tcttgcgaag gatagtggga ttgtgcgtca tcccttacgt
5341 cagtggagat atcacatcaa tccacttgct ttgaagacgt ggttggaacg tcttcttttt
5401 ccacgatgct cctcgtgggt gggggtccat ctttgggacc actgtcggca gaggcatctt
5461 gaacgatagc ctttccttta tcgcaatgat ggcatttgta ggtgccacct tccttttcta
5521 ctgtcctttt gatgaagtga cagatagctg ggcaatggaa tccgaggagg tttcccgata
5581 ttaccctttg ttgaaaagtc tcaatagccc tttggtcttc tgagactgta tctttgatat
5641 tcttggagta gacgagagtg tcgtgctcca ccatgttatc acatcaatcc acttgctttg
5701 aagacgtggt tggaacgtct tctttttcca cgatgctcct cgtgggtggg ggtccatctt
5761 tgggaccact gtcggcagag gcatcttgaa cgatagcctt tcctttatcg caatgatggc
5821 atttgtaggt gccaccttcc ttttctactg tccttttgat gaagtgacag atagctgggc
5881 aatggaatcc gaggaggttt cccgatatta ccctttgttg aaaagtctca atagcccttt
5941 ggtcttctga gactgtatct ttgatattct tggagtagac gagagtgtcg tgctccacca
6001 tgtcgactat cggacgagct caaagtttaa cgcgttagca gaaggcatgt tgttgtgact
6061 ccgaggggtt gcctcaaact ctatcttata accggcgtgg aggcatggag gcaggggtat
6121 tttggtcatt ttaatagata gtggaaaatg acgtggaatt tacttaaaga cgaagtcttt
6181 gcgacaaggg ggggcccacg ccgaatttaa tattaccggc gtggcccccc cttatcgcga
6241 gtgctttagc acgagcggtc cagatttaaa gtagaaaatt tcccgcccac tagggttaaa
6301 ggtgttcaca ctataaaagc atatacgatg tgatggtatt tgatggagcg tatattgtat
6361 caggtatttc cgttggatac gaattattcg tacgaccctc ggcgcgccga gaacttccag
6421 agctttgggt acgtcacgtg gctcgagcgc gtagtcctcg gtaatatcgc agaacaaaag
6481 tacctgatat cgagtgtact tcaagtcagt gggaaatcaa taaaatgatt attttatgaa
6541 tatatttcat tgtgcaagta gatagaaatt acatatgtta cataacacac gaaataaaca
6601 aaaaaagaca atccaaaaac aaacacccca aaaaaaataa tcactttaga taaactcgta
6661 tgaggagagg cacgttcagt gactcgacga ttcccgagca aaaaaagtct ccccgtcaca
6721 catgtagtgg gtgacgcaat tatctttaaa gtaatccttc tgttgacttg tcattgataa
6781 catccagtct tcgtcaggat tgcaaagaat tatagaaggg atcccacctt ttattttctt
6841 cttttttcca tatttagggt tgacagtgaa atcagactgg caacctatta attgcttcca
6901 caatgggacg aacttgaagg ggatgtcgtc gatgatatta taggtggcgt gttcatcgta
6961 gttggtgaaa tcgatggtac cgttccaata gttgtgtcgt ccgagacttc tagcccaggt
7021 ggtctttccg gtacgagttg gtccgcagat gtagaggctg gggtgtcgga ttccattcct
7081 tccattgtcc ttgttaaatc ggccatccat tcaaggtcag attgagcttg ttggtatgag
7141 acaggatgta tgtaagtata agcgtctatg cttacatggt atagatgggt ttccctccag
7201 gagtgtagat cttcgtggca gcgaagatct gattctgtga agggcgacac atacggttca
7261 ggttgtggag ggaataattt gttggctgaa tattccagcc attgaagctt tgttgcccat
7321 tcatgaggga attcttcctt gatcatgtca agatattcct ccttagacgt tgcagtctgg
7381 ataatagttc tccatcgtgc gtcagatttg cgaggagaaa ccttatgatc tcggaaatct
7441 cctctggttt taatatctcc gtcctttgat atgtaatcaa ggacttgttt agagtttcta
7501 gctggctgga tattagggtg atttccttca aaatcgaaaa aagaaggatc cctaatacaa
7561 ggttttttat caagctggag aagagcatga tagtgggtag tgccatcttg atgaagctca
7621 gaagcaacac caaggaagaa aataagaaaa ggtgtgagtt tctcccagag aaactggaat
7681 aaatcatctc tttgagatga gcacttggga taggtaagga aaacatattt agattggagt
7741 ctgaagttct tactagcaga aggcatgttg ttgtgactcc gaggggttgc ctcaaactct
7801 atcttataac cggcgtggag gcatggaggc aggggtattt tggtcatttt aatagatagt
7861 ggaaaatgac gtggaattta cttaaagacg aagtctttgc gacaaggggg ggcccacgcc
7921 gaatttaata ttaccggcgt ggccccccct tatcgcgagt gctttagcac gagcggtcca
7981 gatttaaagt agaaaatttc ccgcccacta gggttaaagg tgttcacact ataaaagcat
8041 atacgatgtg atggtatttg atggagcgta tattgtatca ggtatttccg ttggatacga
8101 attattcgta cgaccctcat agtttaaact atcagtgttt gacaggatat attggcgggt
8161 aaacctaaga gaaaagagcg tttattagaa taacggatat ttaaaagggc gtgaaaaggt
8221 ttatccgttc gtccatttgt atgtgcatgc caaccacagg gttcccctcg ggatcaaagt
8281 actttgatcc aacccctccg ctgctatagt gcagtcggct tctgacgttc agtgcagccg
8341 tcttctgaaa acgacatgtc gcacaagtcc taagttacgc gacaggctgc cgccctgccc
8401 ttttcctggc gttttcttgt cgcgtgtttt agtcgcataa agtagaatac ttgcgactag
8461 aaccggagac attacgccat gaacaagagc gccgccgctg gcctgctggg ctatgcccgc
8521 gtcagcaccg acgaccagga cttgaccaac caacgggccg aactgcacgc ggccggctgc
8581 accaagctgt tttccgagaa gatcaccggc accaggcgcg accgcccgga gctggccagg
8641 atgcttgacc acctacgccc tggcgacgtt gtgacagtga ccaggctaga ccgcctggcc
8701 cgcagcaccc gcgacctact ggacattgcc gagcgcatcc aggaggccgg cgcgggcctg
8761 cgtagcctgg cagagccgtg ggccgacacc accacgccgg ccggccgcat ggtgttgacc
8821 gtgttcgccg gcattgccga gttcgagcgt tccctaatca tcgaccgcac ccggagcggg
8881 cgcgaggccg ccaaggcccg aggcgtgaag tttggccccc gccctaccct caccccggca
8941 cagatcgcgc acgcccgcga gctgatcgac caggaaggcc gcaccgtgaa agaggcggct
9001 gcactgcttg gcgtgcatcg ctcgaccctg taccgcgcac ttgagcgcag cgaggaagtg
9061 acgcccaccg aggccaggcg gcgcggtgcc ttccgtgagg acgcattgac cgaggccgac
9121 gccctggcgg ccgccgagaa tgaacgccaa gaggaacaag catgaaaccg caccaggacg
9181 gccaggacga accgtttttc attaccgaag agatcgaggc ggagatgatc gcggccgggt
9241 acgtgttcga gccgcccgcg cacggctcaa ccgtgcggct gcatgaaatc ctggccggtt
9301 tgtctgatgc caagctggcg gcctggccgg ccagcttggc cgctgaagaa accgagcgcc
9361 gccgtctaaa aaggtgatgt gtatttgagt aaaacagctt gcgtcatgcg gtcgctgcgt
9421 atatgatgcg atgagtaaat aaacaaatac gcaaggggaa cgcatgaagg ttatcgctgt
9481 acttaaccag aaaggcgggt caggcaagac gaccatcgca acccatctag cccgcgccct
9541 gcaactcgcc ggggccgatg ttctgttagt cgattccgat ccccagggca gtgcccgcga
9601 ttgggcggcc gtgcgggaag atcaaccgct aaccgttgtc ggcatcgacc gcccgacgat
9661 tgaccgcgac gtgaaggcca tcggccggcg cgacttcgta gtgatcgacg gagcgcccca
9721 ggcggcggac ttggctgtgt ccgcgatcaa ggcagccgac ttcgtgctga ttccggtgca
9781 gccaagccct tacgacatat gggccaccgc cgacctggtg gagctggtta agcagcgcat
9841 tgaggtcacg gatggaaggc tacaagcggc ctttgtcgtg tcgcgggcga tcaaaggcac
9901 gcgcatcggc ggtgaggttg ccgaggcgct ggccgggtac gagctgccca ttcttgagtc
9961 ccgtatcacg cagcgcgtga gctacccagg cactgccgcc gccggcacaa ccgttcttga
10021 atcagaaccc gagggcgacg ctgcccgcga ggtccaggcg ctggccgctg aaattaaatc
10081 aaaactcatt tgagttaatg aggtaaagag aaaatgagca aaagcacaaa cacgctaagt
10141 gccggccgtc cgagcgcacg cagcagcaag gctgcaacgt tggccagcct ggcagacacg
10201 ccagccatga agcgggtcaa ctttcagttg ccggcggagg atcacaccaa gctgaagatg
10261 tacgcggtac gccaaggcaa gaccattacc gagctgctat ctgaatacat cgcgcagcta
10321 ccagagtaaa tgagcaaatg aataaatgag tagatgaatt ttagcggcta aaggaggcgg
10381 catggaaaat caagaacaac caggcaccga cgccgtggaa tgccccatgt gtggaggaac
10441 gggcggttgg ccaggcgtaa gcggctgggt tgtctgccgg ccctgcaatg gcactggaac
10501 ccccaagccc gaggaatcgg cgtgacggtc g
//