LOCUS Exported 8861 bp ds-DNA circular SYN 11-JAN-2016
DEFINITION Agrobacterium binary vector for plant transformation, with
hygromycin- and kanamycin-resistance genes.
ACCESSION AF234301
VERSION .
KEYWORDS pCAMBIA1380
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8861)
AUTHORS Cambia
TITLE Direct Submission
JOURNAL Exported Wednesday, September 14, 2016 from SnapGene Viewer 3.2.1
http://www.miaolingbio.com
COMMENT The GenBank record was corrected by inserting a G at position 2718.
FEATURES Location/Qualifiers
source 1..8861
/organism="synthetic DNA construct"
/lab_host="Plant Cells"
/mol_type="other DNA"
CDS 6..23
/codon_start=1
/product="6xHis affinity tag"
/note="6xHis"
/translation="HHHHHH"
terminator 58..310
/note="NOS terminator"
/note="nopaline synthase terminator and poly(A) signal"
misc_feature 332..356
/note="RB T-DNA repeat"
/note="right border repeat from nopaline C58 T-DNA"
CDS 1656..2285
/codon_start=1
/product="stability protein from Pseudomonas plasmid pVS1"
/note="pVS1 StaA"
/translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
CDS 2714..3787
/codon_start=1
/product="replication protein from Pseudomonas plasmid
pVS1"
/note="pVS1 RepA"
/translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
LVMRYRNLIEGEASAGS"
rep_origin 3853..4047
/note="pVS1 oriV"
/note="origin of replication for the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
misc_feature 4391..4531
/note="bom"
/note="basis of mobility region from pBR322"
rep_origin complement(4717..5305)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(5392..6186)
/codon_start=1
/gene="aphA-3"
/product="aminoglycoside phosphotransferase"
/note="KanR"
/note="confers resistance to kanamycin"
/translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
misc_feature 6611..6635
/note="LB T-DNA repeat"
/note="left border repeat from nopaline C58 T-DNA"
polyA_signal 6713..6887
/note="CaMV poly(A) signal"
/note="cauliflower mosaic virus polyadenylation signal"
CDS complement(6927..7952)
/codon_start=1
/product="hygromycin B phosphotransferase"
/note="HygR"
/note="confers resistance to hygromycin"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
K"
promoter complement(8020..8697)
/note="CaMV 35S promoter (enhanced)"
/note="cauliflower mosaic virus 35S promoter with a
duplicated enhancer region"
misc_feature 8786..8821
/note="MCS"
/note="multiple cloning site from pUC8"
ORIGIN
1 ctagccacca ccaccaccac cacgtgtgaa ttacaggtga ccagctcgaa tttccccgat
61 cgttcaaaca tttggcaata aagtttctta agattgaatc ctgttgccgg tcttgcgatg
121 attatcatat aatttctgtt gaattacgtt aagcatgtaa taattaacat gtaatgcatg
181 acgttattta tgagatgggt ttttatgatt agagtcccgc aattatacat ttaatacgcg
241 atagaaaaca aaatatagcg cgcaaactag gataaattat cgcgcgcggt gtcatctatg
301 ttactagatc gggaattaaa ctatcagtgt ttgacaggat atattggcgg gtaaacctaa
361 gagaaaagag cgtttattag aataacggat atttaaaagg gcgtgaaaag gtttatccgt
421 tcgtccattt gtatgtgcat gccaaccaca gggttcccct cgggatcaaa gtactttgat
481 ccaacccctc cgctgctata gtgcagtcgg cttctgacgt tcagtgcagc cgtcttctga
541 aaacgacatg tcgcacaagt cctaagttac gcgacaggct gccgccctgc ccttttcctg
601 gcgttttctt gtcgcgtgtt ttagtcgcat aaagtagaat acttgcgact agaaccggag
661 acattacgcc atgaacaaga gcgccgccgc tggcctgctg ggctatgccc gcgtcagcac
721 cgacgaccag gacttgacca accaacgggc cgaactgcac gcggccggct gcaccaagct
781 gttttccgag aagatcaccg gcaccaggcg cgaccgcccg gagctggcca ggatgcttga
841 ccacctacgc cctggcgacg ttgtgacagt gaccaggcta gaccgcctgg cccgcagcac
901 ccgcgaccta ctggacattg ccgagcgcat ccaggaggcc ggcgcgggcc tgcgtagcct
961 ggcagagccg tgggccgaca ccaccacgcc ggccggccgc atggtgttga ccgtgttcgc
1021 cggcattgcc gagttcgagc gttccctaat catcgaccgc acccggagcg ggcgcgaggc
1081 cgccaaggcc cgaggcgtga agtttggccc ccgccctacc ctcaccccgg cacagatcgc
1141 gcacgcccgc gagctgatcg accaggaagg ccgcaccgtg aaagaggcgg ctgcactgct
1201 tggcgtgcat cgctcgaccc tgtaccgcgc acttgagcgc agcgaggaag tgacgcccac
1261 cgaggccagg cggcgcggtg ccttccgtga ggacgcattg accgaggccg acgccctggc
1321 ggccgccgag aatgaacgcc aagaggaaca agcatgaaac cgcaccagga cggccaggac
1381 gaaccgtttt tcattaccga agagatcgag gcggagatga tcgcggccgg gtacgtgttc
1441 gagccgcccg cgcacgtctc aaccgtgcgg ctgcatgaaa tcctggccgg tttgtctgat
1501 gccaagctgg cggcctggcc ggccagcttg gccgctgaag aaaccgagcg ccgccgtcta
1561 aaaaggtgat gtgtatttga gtaaaacagc ttgcgtcatg cggtcgctgc gtatatgatg
1621 cgatgagtaa ataaacaaat acgcaagggg aacgcatgaa ggttatcgct gtacttaacc
1681 agaaaggcgg gtcaggcaag acgaccatcg caacccatct agcccgcgcc ctgcaactcg
1741 ccggggccga tgttctgtta gtcgattccg atccccaggg cagtgcccgc gattgggcgg
1801 ccgtgcggga agatcaaccg ctaaccgttg tcggcatcga ccgcccgacg attgaccgcg
1861 acgtgaaggc catcggccgg cgcgacttcg tagtgatcga cggagcgccc caggcggcgg
1921 acttggctgt gtccgcgatc aaggcagccg acttcgtgct gattccggtg cagccaagcc
1981 cttacgacat atgggccacc gccgacctgg tggagctggt taagcagcgc attgaggtca
2041 cggatggaag gctacaagcg gcctttgtcg tgtcgcgggc gatcaaaggc acgcgcatcg
2101 gcggtgaggt tgccgaggcg ctggccgggt acgagctgcc cattcttgag tcccgtatca
2161 cgcagcgcgt gagctaccca ggcactgccg ccgccggcac aaccgttctt gaatcagaac
2221 ccgagggcga cgctgcccgc gaggtccagg cgctggccgc tgaaattaaa tcaaaactca
2281 tttgagttaa tgaggtaaag agaaaatgag caaaagcaca aacacgctaa gtgccggccg
2341 tccgagcgca cgcagcagca aggctgcaac gttggccagc ctggcagaca cgccagccat
2401 gaagcgggtc aactttcagt tgccggcgga ggatcacacc aagctgaaga tgtacgcggt
2461 acgccaaggc aagaccatta ccgagctgct atctgaatac atcgcgcagc taccagagta
2521 aatgagcaaa tgaataaatg agtagatgaa ttttagcggc taaaggaggc ggcatggaaa
2581 atcaagaaca accaggcacc gacgccgtgg aatgccccat gtgtggagga acgggcggtt
2641 ggccaggcgt aagcggctgg gttgtctgcc ggccctgcaa tggcactgga acccccaagc
2701 ccgaggaatc ggcgtgagcg gtcgcaaacc atccggcccg gtacaaatcg gcgcggcgct
2761 gggtgatgac ctggtggaga agttgaaggc cgcgcaggcc gcccagcggc aacgcatcga
2821 ggcagaagca cgccccggtg aatcgtggca agcggccgct gatcgaatcc gcaaagaatc
2881 ccggcaaccg ccggcagccg gtgcgccgtc gattaggaag ccgcccaagg gcgacgagca
2941 accagatttt ttcgttccga tgctctatga cgtgggcacc cgcgatagtc gcagcatcat
3001 ggacgtggcc gttttccgtc tgtcgaagcg tgaccgacga gctggcgagg tgatccgcta
3061 cgagcttcca gacgggcacg tagaggtttc cgcagggccg gccggcatgg ccagtgtgtg
3121 ggattacgac ctggtactga tggcggtttc ccatctaacc gaatccatga accgataccg
3181 ggaagggaag ggagacaagc ccggccgcgt gttccgtcca cacgttgcgg acgtactcaa
3241 gttctgccgg cgagccgatg gcggaaagca gaaagacgac ctggtagaaa cctgcattcg
3301 gttaaacacc acgcacgttg ccatgcagcg tacgaagaag gccaagaacg gccgcctggt
3361 gacggtatcc gagggtgaag ccttgattag ccgctacaag atcgtaaaga gcgaaaccgg
3421 gcggccggag tacatcgaga tcgagctagc tgattggatg taccgcgaga tcacagaagg
3481 caagaacccg gacgtgctga cggttcaccc cgattacttt ttgatcgatc ccggcatcgg
3541 ccgttttctc taccgcctgg cacgccgcgc cgcaggcaag gcagaagcca gatggttgtt
3601 caagacgatc tacgaacgca gtggcagcgc cggagagttc aagaagttct gtttcaccgt
3661 gcgcaagctg atcgggtcaa atgacctgcc ggagtacgat ttgaaggagg aggcggggca
3721 ggctggcccg atcctagtca tgcgctaccg caacctgatc gagggcgaag catccgccgg
3781 ttcctaatgt acggagcaga tgctagggca aattgcccta gcaggggaaa aaggtcgaaa
3841 aggtctcttt cctgtggata gcacgtacat tgggaaccca aagccgtaca ttgggaaccg
3901 gaacccgtac attgggaacc caaagccgta cattgggaac cggtcacaca tgtaagtgac
3961 tgatataaaa gagaaaaaag gcgatttttc cgcctaaaac tctttaaaac ttattaaaac
4021 tcttaaaacc cgcctggcct gtgcataact gtctggccag cgcacagccg aagagctgca
4081 aaaagcgcct acccttcggt cgctgcgctc cctacgcccc gccgcttcgc gtcggcctat
4141 cgcggccgct ggccgctcaa aaatggctgg cctacggcca ggcaatctac cagggcgcgg
4201 acaagccgcg ccgtcgccac tcgaccgccg gcgcccacat caaggcaccc tgcctcgcgc
4261 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
4321 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
4381 ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat actggcttaa
4441 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca
4501 cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc tcactgactc
4561 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
4621 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
4681 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
4741 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
4801 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
4861 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
4921 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
4981 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
5041 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
5101 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
5161 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
5221 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
5281 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
5341 tcagtggaac gaaaactcac gttaagggat tttggtcatg cattctaggt actaaaacaa
5401 ttcatccagt aaaatataat attttatttt ctcccaatca ggcttgatcc ccagtaagtc
5461 aaaaaatagc tcgacatact gttcttcccc gatatcctcc ctgatcgacc ggacgcagaa
5521 ggcaatgtca taccacttgt ccgccctgcc gcttctccca agatcaataa agccacttac
5581 tttgccatct ttcacaaaga tgttgctgtc tcccaggtcg ccgtgggaaa agacaagttc
5641 ctcttcgggc ttttccgtct ttaaaaaatc atacagctcg cgcggatctt taaatggagt
5701 gtcttcttcc cagttttcgc aatccacatc ggccagatcg ttattcagta agtaatccaa
5761 ttcggctaag cggctgtcta agctattcgt atagggacaa tccgatatgt cgatggagtg
5821 aaagagcctg atgcactccg catacagctc gataatcttt tcagggcttt gttcatcttc
5881 atactcttcc gagcaaagga cgccatcggc ctcactcatg agcagattgc tccagccatc
5941 atgccgttca aagtgcagga cctttggaac aggcagcttt ccttccagcc atagcatcat
6001 gtccttttcc cgttccacat cataggtggt ccctttatac cggctgtccg tcatttttaa
6061 atataggttt tcattttctc ccaccagctt atatacctta gcaggagaca ttccttccgt
6121 atcttttacg cagcggtatt tttcgatcag ttttttcaat tccggtgata ttctcatttt
6181 agccatttat tatttccttc ctcttttcta cagtatttaa agatacccca agaagctaat
6241 tataacaaga cgaactccaa ttcactgttc cttgcattct aaaaccttaa ataccagaaa
6301 acagcttttt caaagttgtt ttcaaagttg gcgtataaca tagtatcgac ggagccgatt
6361 ttgaaaccgc ggtgatcaca ggcagcaacg ctctgtcatc gttacaatca acatgctacc
6421 ctccgcgaga tcatccgtgt ttcaaacccg gcagcttagt tgccgttctt ccgaatagca
6481 tcggtaacat gagcaaagtc tgccgcctta caacggctct cccgctgacg ccgtcccgga
6541 ctgatgggct gcctgtatcg agtggtgatt ttgtgccgag ctgccggtcg gggagctgtt
6601 ggctggctgg tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata
6661 acacattgcg gacgttttta atgtactgaa ttaacgccga attaattcgg gggatctgga
6721 ttttagtact ggattttggt tttaggaatt agaaatttta ttgatagaag tattttacaa
6781 atacaaatac atactaaggg tttcttatat gctcaacaca tgagcgaaac cctataggaa
6841 ccctaattcc cttatctggg aactactcac acattattat ggagaaactc gagcttgtcg
6901 atcgacagat ccggtcggca tctactctat ttctttgccc tcggacgagt gctggggcgt
6961 cggtttccac tatcggcgag tacttctaca cagccatcgg tccagacggc cgcgcttctg
7021 cgggcgattt gtgtacgccc gacagtcccg gctccggatc ggacgattgc gtcgcatcga
7081 ccctgcgccc aagctgcatc atcgaaattg ccgtcaacca agctctgata gagttggtca
7141 agaccaatgc ggagcatata cgcccggagt cgtggcgatc ctgcaagctc cggatgcctc
7201 cgctcgaagt agcgcgtctg ctgctccata caagccaacc acggcctcca gaagaagatg
7261 ttggcgacct cgtattggga atccccgaac atcgcctcgc tccagtcaat gaccgctgtt
7321 atgcggccat tgtccgtcag gacattgttg gagccgaaat ccgcgtgcac gaggtgccgg
7381 acttcggggc agtcctcggc ccaaagcatc agctcatcga gagcctgcgc gacggacgca
7441 ctgacggtgt cgtccatcac agtttgccag tgatacacat ggggatcagc aatcgcgcat
7501 atgaaatcac gccatgtagt gtattgaccg attccttgcg gtccgaatgg gccgaacccg
7561 ctcgtctggc taagatcggc cgcagcgatc gcatccatag cctccgcgac cggttgtaga
7621 acagcgggca gttcggtttc aggcaggtct tgcaacgtga caccctgtgc acggcgggag
7681 atgcaatagg tcaggctctc gctaaactcc ccaatgtcaa gcacttccgg aatcgggagc
7741 gcggccgatg caaagtgccg ataaacataa cgatctttgt agaaaccatc ggcgcagcta
7801 tttacccgca ggacatatcc acgccctcct acatcgaagc tgaaagcacg agattcttcg
7861 ccctccgaga gctgcatcag gtcggagacg ctgtcgaact tttcgatcag aaacttctcg
7921 acagacgtcg cggtgagttc aggctttttc atatctcatt gccccccggg atctgcgaaa
7981 gctcgagaga gatagatttg tagagagaga ctggtgattt cagcgtgtcc tctccaaatg
8041 aaatgaactt ccttatatag aggaaggtct tgcgaaggat agtgggattg tgcgtcatcc
8101 cttacgtcag tggagatatc acatcaatcc acttgctttg aagacgtggt tggaacgtct
8161 tctttttcca cgatgctcct cgtgggtggg ggtccatctt tgggaccact gtcggcagag
8221 gcatcttgaa cgatagcctt tcctttatcg caatgatggc atttgtaggt gccaccttcc
8281 ttttctactg tccttttgat gaagtgacag atagctgggc aatggaatcc gaggaggttt
8341 cccgatatta ccctttgttg aaaagtctca atagcccttt ggtcttctga gactgtatct
8401 ttgatattct tggagtagac gagagtgtcg tgctccacca tgttatcaca tcaatccact
8461 tgctttgaag acgtggttgg aacgtcttct ttttccacga tgctcctcgt gggtgggggt
8521 ccatctttgg gaccactgtc ggcagaggca tcttgaacga tagcctttcc tttatcgcaa
8581 tgatggcatt tgtaggtgcc accttccttt tctactgtcc ttttgatgaa gtgacagata
8641 gctgggcaat ggaatccgag gaggtttccc gatattaccc tttgttgaaa agtctcaata
8701 gccctttggt cttctgagac tgtatctttg atattcttgg agtagacgag agtgtcgtgc
8761 tccaccatgt tgggcccggc gcgccgaatt cccggggatc cgtcgacctg cagccaagct
8821 ttaagaggag tccaccatgg tagatctgac tagtgttaac g
//