LOCUS Exported 10657 bp ds-DNA circular SYN 14-SEP-2016
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS Untitled 5
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10657)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Wednesday, September 14, 2016 from SnapGene Viewer 3.2.1
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..10657
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS 20..1825
/codon_start=1
/gene="uidA (or gusA) from E. coli"
/product="beta-glucuronidase"
/note="GUS"
/translation="LRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAIA
VPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEVM
EHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHDF
FNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQV
VATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGQQFL
INHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDWA
DEHGIVVIDETAAVGFQLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARDK
NHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISDL
FDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMYT
DMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRKP
KSAAFLLQKRWTGMNFGEKPQQGGKQ"
CDS 1832..1849
/codon_start=1
/product="6xHis affinity tag"
/note="6xHis"
/translation="HHHHHH"
terminator 1881..2133
/note="NOS terminator"
/note="nopaline synthase terminator and poly(A) signal"
misc_feature 2155..2179
/note="RB T-DNA repeat"
/note="right border repeat from nopaline C58 T-DNA"
CDS 3479..4108
/codon_start=1
/product="stability protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/note="pVS1 StaA"
/translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
CDS 4542..5609
/codon_start=1
/product="replication protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/note="pVS1 RepA"
/translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
MRYRNLIEGEASAGS"
rep_origin 5675..5869
/note="pVS1 oriV"
/note="origin of replication for the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
misc_feature 6213..6353
/note="bom"
/note="basis of mobility region from pBR322"
rep_origin complement(6539..7127)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(7214..8008)
/codon_start=1
/gene="aphA-3"
/product="aminoglycoside phosphotransferase"
/note="KanR"
/note="confers resistance to kanamycin"
/translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
misc_feature 8433..8457
/note="LB T-DNA repeat"
/note="left border repeat from nopaline C58 T-DNA"
polyA_signal 8535..8709
/note="CaMV poly(A) signal"
/note="cauliflower mosaic virus polyadenylation signal"
CDS complement(8749..9774)
/codon_start=1
/gene="aph(4)-Ia"
/product="aminoglycoside phosphotransferase from E. coli"
/note="HygR"
/note="confers resistance to hygromycin"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
K"
promoter complement(9842..10519)
/note="CaMV 35S promoter (enhanced)"
/note="cauliflower mosaic virus 35S promoter with a
duplicated enhancer region"
ORIGIN
1 catggtagat ctgactagtt tacgtcctgt agaaacccca acccgtgaaa tcaaaaaact
61 cgacggcctg tgggcattca gtctggatcg cgaaaactgt ggaattgatc agcgttggtg
121 ggaaagcgcg ttacaagaaa gccgggcaat tgctgtgcca ggcagtttta acgatcagtt
181 cgccgatgca gatattcgta attatgcggg caacgtctgg tatcagcgcg aagtctttat
241 accgaaaggt tgggcaggcc agcgtatcgt gctgcgtttc gatgcggtca ctcattacgg
301 caaagtgtgg gtcaataatc aggaagtgat ggagcatcag ggcggctata cgccatttga
361 agccgatgtc acgccgtatg ttattgccgg gaaaagtgta cgtatcaccg tttgtgtgaa
421 caacgaactg aactggcaga ctatcccgcc gggaatggtg attaccgacg aaaacggcaa
481 gaaaaagcag tcttacttcc atgatttctt taactatgcc ggaatccatc gcagcgtaat
541 gctctacacc acgccgaaca cctgggtgga cgatatcacc gtggtgacgc atgtcgcgca
601 agactgtaac cacgcgtctg ttgactggca ggtggtggcc aatggtgatg tcagcgttga
661 actgcgtgat gcggatcaac aggtggttgc aactggacaa ggcactagcg ggactttgca
721 agtggtgaat ccgcacctct ggcaaccggg tgaaggttat ctctatgaac tgtgcgtcac
781 agccaaaagc cagacagagt gtgatatcta cccgcttcgc gtcggcatcc ggtcagtggc
841 agtgaagggc caacagttcc tgattaacca caaaccgttc tactttactg gctttggtcg
901 tcatgaagat gcggacttac gtggcaaagg attcgataac gtgctgatgg tgcacgacca
961 cgcattaatg gactggattg gggccaactc ctaccgtacc tcgcattacc cttacgctga
1021 agagatgctc gactgggcag atgaacatgg catcgtggtg attgatgaaa ctgctgctgt
1081 cggctttcag ctgtctttag gcattggttt cgaagcgggc aacaagccga aagaactgta
1141 cagcgaagag gcagtcaacg gggaaactca gcaagcgcac ttacaggcga ttaaagagct
1201 gatagcgcgt gacaaaaacc acccaagcgt ggtgatgtgg agtattgcca acgaaccgga
1261 tacccgtccg caaggtgcac gggaatattt cgcgccactg gcggaagcaa cgcgtaaact
1321 cgacccgacg cgtccgatca cctgcgtcaa tgtaatgttc tgcgacgctc acaccgatac
1381 catcagcgat ctctttgatg tgctgtgcct gaaccgttat tacggatggt atgtccaaag
1441 cggcgatttg gaaacggcag agaaggtact ggaaaaagaa cttctggcct ggcaggagaa
1501 actgcatcag ccgattatca tcaccgaata cggcgtggat acgttagccg ggctgcactc
1561 aatgtacacc gacatgtgga gtgaagagta tcagtgtgca tggctggata tgtatcaccg
1621 cgtctttgat cgcgtcagcg ccgtcgtcgg tgaacaggta tggaatttcg ccgattttgc
1681 gacctcgcaa ggcatattgc gcgttggcgg taacaagaaa gggatcttca ctcgcgaccg
1741 caaaccgaag tcggcggctt ttctgctgca aaaacgctgg actggcatga acttcggtga
1801 aaaaccgcag cagggaggca aacaagctag ccaccaccac caccaccacg tgtgaattgg
1861 tgaccagctc gaatttcccc gatcgttcaa acatttggca ataaagtttc ttaagattga
1921 atcctgttgc cggtcttgcg atgattatca tataatttct gttgaattac gttaagcatg
1981 taataattaa catgtaatgc atgacgttat ttatgagatg ggtttttatg attagagtcc
2041 cgcaattata catttaatac gcgatagaaa acaaaatata gcgcgcaaac taggataaat
2101 tatcgcgcgc ggtgtcatct atgttactag atcgggaatt aaactatcag tgtttgacag
2161 gatatattgg cgggtaaacc taagagaaaa gagcgtttat tagaataacg gatatttaaa
2221 agggcgtgaa aaggtttatc cgttcgtcca tttgtatgtg catgccaacc acagggttcc
2281 cctcgggatc aaagtacttt gatccaaccc ctccgctgct atagtgcagt cggcttctga
2341 cgttcagtgc agccgtcttc tgaaaacgac atgtcgcaca agtcctaagt tacgcgacag
2401 gctgccgccc tgcccttttc ctggcgtttt cttgtcgcgt gttttagtcg cataaagtag
2461 aatacttgcg actagaaccg gagacattac gccatgaaca agagcgccgc cgctggcctg
2521 ctgggctatg cccgcgtcag caccgacgac caggacttga ccaaccaacg ggccgaactg
2581 cacgcggccg gctgcaccaa gctgttttcc gagaagatca ccggcaccag gcgcgaccgc
2641 ccggagctgg ccaggatgct tgaccaccta cgccctggcg acgttgtgac agtgaccagg
2701 ctagaccgcc tggcccgcag cacccgcgac ctactggaca ttgccgagcg catccaggag
2761 gccggcgcgg gcctgcgtag cctggcagag ccgtgggccg acaccaccac gccggccggc
2821 cgcatggtgt tgaccgtgtt cgccggcatt gccgagttcg agcgttccct aatcatcgac
2881 cgcacccgga gcgggcgcga ggccgccaag gcccgaggcg tgaagtttgg cccccgccct
2941 accctcaccc cggcacagat cgcgcacgcc cgcgagctga tcgaccagga aggccgcacc
3001 gtgaaagagg cggctgcact gcttggcgtg catcgctcga ccctgtaccg cgcacttgag
3061 cgcagcgagg aagtgacgcc caccgaggcc aggcggcgcg gtgccttccg tgaggacgca
3121 ttgaccgagg ccgacgccct ggcggccgcc gagaatgaac gccaagagga acaagcatga
3181 aaccgcacca ggacggccag gacgaaccgt ttttcattac cgaagagatc gaggcggaga
3241 tgatcgcggc cgggtacgtg ttcgagccgc ccgcgcacgt ctcaaccgtg cggctgcatg
3301 aaatcctggc cggtttgtct gatgccaagc tggcggcctg gccggccagc ttggccgctg
3361 aagaaaccga gcgccgccgt ctaaaaaggt gatgtgtatt tgagtaaaac agcttgcgtc
3421 atgcggtcgc tgcgtatatg atgcgatgag taaataaaca aatacgcaag gggaacgcat
3481 gaaggttatc gctgtactta accagaaagg cgggtcaggc aagacgacca tcgcaaccca
3541 tctagcccgc gccctgcaac tcgccggggc cgatgttctg ttagtcgatt ccgatcccca
3601 gggcagtgcc cgcgattggg cggccgtgcg ggaagatcaa ccgctaaccg ttgtcggcat
3661 cgaccgcccg acgattgacc gcgacgtgaa ggccatcggc cggcgcgact tcgtagtgat
3721 cgacggagcg ccccaggcgg cggacttggc tgtgtccgcg atcaaggcag ccgacttcgt
3781 gctgattccg gtgcagccaa gcccttacga catatgggcc accgccgacc tggtggagct
3841 ggttaagcag cgcattgagg tcacggatgg aaggctacaa gcggcctttg tcgtgtcgcg
3901 ggcgatcaaa ggcacgcgca tcggcggtga ggttgccgag gcgctggccg ggtacgagct
3961 gcccattctt gagtcccgta tcacgcagcg cgtgagctac ccaggcactg ccgccgccgg
4021 cacaaccgtt cttgaatcag aacccgaggg cgacgctgcc cgcgaggtcc aggcgctggc
4081 cgctgaaatt aaatcaaaac tcatttgagt taatgaggta aagagaaaat gagcaaaagc
4141 acaaacacgc taagtgccgg ccgtccgagc gcacgcagca gcaaggctgc aacgttggcc
4201 agcctggcag acacgccagc catgaagcgg gtcaactttc agttgccggc ggaggatcac
4261 accaagctga agatgtacgc ggtacgccaa ggcaagacca ttaccgagct gctatctgaa
4321 tacatcgcgc agctaccaga gtaaatgagc aaatgaataa atgagtagat gaattttagc
4381 ggctaaagga ggcggcatgg aaaatcaaga acaaccaggc accgacgccg tggaatgccc
4441 catgtgtgga ggaacgggcg gttggccagg cgtaagcggc tgggttgtct gccggccctg
4501 caatggcact ggaaccccca agcccgagga atcggcgtga cggtcgcaaa ccatccggcc
4561 cggtacaaat cggcgcggcg ctgggtgatg acctggtgga gaagttgaag gccgcgcagg
4621 ccgcccagcg gcaacgcatc gaggcagaag cacgccccgg tgaatcgtgg caagcggccg
4681 ctgatcgaat ccgcaaagaa tcccggcaac cgccggcagc cggtgcgccg tcgattagga
4741 agccgcccaa gggcgacgag caaccagatt ttttcgttcc gatgctctat gacgtgggca
4801 cccgcgatag tcgcagcatc atggacgtgg ccgttttccg tctgtcgaag cgtgaccgac
4861 gagctggcga ggtgatccgc tacgagcttc cagacgggca cgtagaggtt tccgcagggc
4921 cggccggcat ggccagtgtg tgggattacg acctggtact gatggcggtt tcccatctaa
4981 ccgaatccat gaaccgatac cgggaaggga agggagacaa gcccggccgc gtgttccgtc
5041 cacacgttgc ggacgtactc aagttctgcc ggcgagccga tggcggaaag cagaaagacg
5101 acctggtaga aacctgcatt cggttaaaca ccacgcacgt tgccatgcag cgtacgaaga
5161 aggccaagaa cggccgcctg gtgacggtat ccgagggtga agccttgatt agccgctaca
5221 agatcgtaaa gagcgaaacc gggcggccgg agtacatcga gatcgagcta gctgattgga
5281 tgtaccgcga gatcacagaa ggcaagaacc cggacgtgct gacggttcac cccgattact
5341 ttttgatcga tcccggcatc ggccgttttc tctaccgcct ggcacgccgc gccgcaggca
5401 aggcagaagc cagatggttg ttcaagacga tctacgaacg cagtggcagc gccggagagt
5461 tcaagaagtt ctgtttcacc gtgcgcaagc tgatcgggtc aaatgacctg ccggagtacg
5521 atttgaagga ggaggcgggg caggctggcc cgatcctagt catgcgctac cgcaacctga
5581 tcgagggcga agcatccgcc ggttcctaat gtacggagca gatgctaggg caaattgccc
5641 tagcagggga aaaaggtcga aaaggtctct ttcctgtgga tagcacgtac attgggaacc
5701 caaagccgta cattgggaac cggaacccgt acattgggaa cccaaagccg tacattggga
5761 accggtcaca catgtaagtg actgatataa aagagaaaaa aggcgatttt tccgcctaaa
5821 actctttaaa acttattaaa actcttaaaa cccgcctggc ctgtgcataa ctgtctggcc
5881 agcgcacagc cgaagagctg caaaaagcgc ctacccttcg gtcgctgcgc tccctacgcc
5941 ccgccgcttc gcgtcggcct atcgcggccg ctggccgctc aaaaatggct ggcctacggc
6001 caggcaatct accagggcgc ggacaagccg cgccgtcgcc actcgaccgc cggcgcccac
6061 atcaaggcac cctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
6121 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
6181 ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat
6241 agcggagtgt atactggctt aactatgcgg catcagagca gattgtactg agagtgcacc
6301 atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt
6361 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
6421 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
6481 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
6541 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
6601 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
6661 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
6721 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
6781 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
6841 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
6901 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
6961 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
7021 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
7081 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
7141 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
7201 tgcattctag gtactaaaac aattcatcca gtaaaatata atattttatt ttctcccaat
7261 caggcttgat ccccagtaag tcaaaaaata gctcgacata ctgttcttcc ccgatatcct
7321 ccctgatcga ccggacgcag aaggcaatgt cataccactt gtccgccctg ccgcttctcc
7381 caagatcaat aaagccactt actttgccat ctttcacaaa gatgttgctg tctcccaggt
7441 cgccgtggga aaagacaagt tcctcttcgg gcttttccgt ctttaaaaaa tcatacagct
7501 cgcgcggatc tttaaatgga gtgtcttctt cccagttttc gcaatccaca tcggccagat
7561 cgttattcag taagtaatcc aattcggcta agcggctgtc taagctattc gtatagggac
7621 aatccgatat gtcgatggag tgaaagagcc tgatgcactc cgcatacagc tcgataatct
7681 tttcagggct ttgttcatct tcatactctt ccgagcaaag gacgccatcg gcctcactca
7741 tgagcagatt gctccagcca tcatgccgtt caaagtgcag gacctttgga acaggcagct
7801 ttccttccag ccatagcatc atgtcctttt cccgttccac atcataggtg gtccctttat
7861 accggctgtc cgtcattttt aaatataggt tttcattttc tcccaccagc ttatatacct
7921 tagcaggaga cattccttcc gtatctttta cgcagcggta tttttcgatc agttttttca
7981 attccggtga tattctcatt ttagccattt attatttcct tcctcttttc tacagtattt
8041 aaagataccc caagaagcta attataacaa gacgaactcc aattcactgt tccttgcatt
8101 ctaaaacctt aaataccaga aaacagcttt ttcaaagttg ttttcaaagt tggcgtataa
8161 catagtatcg acggagccga ttttgaaacc gcggtgatca caggcagcaa cgctctgtca
8221 tcgttacaat caacatgcta ccctccgcga gatcatccgt gtttcaaacc cggcagctta
8281 gttgccgttc ttccgaatag catcggtaac atgagcaaag tctgccgcct tacaacggct
8341 ctcccgctga cgccgtcccg gactgatggg ctgcctgtat cgagtggtga ttttgtgccg
8401 agctgccggt cggggagctg ttggctggct ggtggcagga tatattgtgg tgtaaacaaa
8461 ttgacgctta gacaacttaa taacacattg cggacgtttt taatgtactg aattaacgcc
8521 gaattaattc gggggatctg gattttagta ctggattttg gttttaggaa ttagaaattt
8581 tattgataga agtattttac aaatacaaat acatactaag ggtttcttat atgctcaaca
8641 catgagcgaa accctatagg aaccctaatt cccttatctg ggaactactc acacattatt
8701 atggagaaac tcgagcttgt cgatcgacag atccggtcgg catctactct atttctttgc
8761 cctcggacga gtgctggggc gtcggtttcc actatcggcg agtacttcta cacagccatc
8821 ggtccagacg gccgcgcttc tgcgggcgat ttgtgtacgc ccgacagtcc cggctccgga
8881 tcggacgatt gcgtcgcatc gaccctgcgc ccaagctgca tcatcgaaat tgccgtcaac
8941 caagctctga tagagttggt caagaccaat gcggagcata tacgcccgga gtcgtggcga
9001 tcctgcaagc tccggatgcc tccgctcgaa gtagcgcgtc tgctgctcca tacaagccaa
9061 ccacggcctc cagaagaaga tgttggcgac ctcgtattgg gaatccccga acatcgcctc
9121 gctccagtca atgaccgctg ttatgcggcc attgtccgtc aggacattgt tggagccgaa
9181 atccgcgtgc acgaggtgcc ggacttcggg gcagtcctcg gcccaaagca tcagctcatc
9241 gagagcctgc gcgacggacg cactgacggt gtcgtccatc acagtttgcc agtgatacac
9301 atggggatca gcaatcgcgc atatgaaatc acgccatgta gtgtattgac cgattccttg
9361 cggtccgaat gggccgaacc cgctcgtctg gctaagatcg gccgcagcga tcgcatccat
9421 agcctccgcg accggttgta gaacagcggg cagttcggtt tcaggcaggt cttgcaacgt
9481 gacaccctgt gcacggcggg agatgcaata ggtcaggctc tcgctaaact ccccaatgtc
9541 aagcacttcc ggaatcggga gcgcggccga tgcaaagtgc cgataaacat aacgatcttt
9601 gtagaaacca tcggcgcagc tatttacccg caggacatat ccacgccctc ctacatcgaa
9661 gctgaaagca cgagattctt cgccctccga gagctgcatc aggtcggaga cgctgtcgaa
9721 cttttcgatc agaaacttct cgacagacgt cgcggtgagt tcaggctttt tcatatctca
9781 ttgccccccg ggatctgcga aagctcgaga gagatagatt tgtagagaga gactggtgat
9841 ttcagcgtgt cctctccaaa tgaaatgaac ttccttatat agaggaaggt cttgcgaagg
9901 atagtgggat tgtgcgtcat cccttacgtc agtggagata tcacatcaat ccacttgctt
9961 tgaagacgtg gttggaacgt cttctttttc cacgatgctc ctcgtgggtg ggggtccatc
10021 tttgggacca ctgtcggcag aggcatcttg aacgatagcc tttcctttat cgcaatgatg
10081 gcatttgtag gtgccacctt ccttttctac tgtccttttg atgaagtgac agatagctgg
10141 gcaatggaat ccgaggaggt ttcccgatat taccctttgt tgaaaagtct caatagccct
10201 ttggtcttct gagactgtat ctttgatatt cttggagtag acgagagtgt cgtgctccac
10261 catgttatca catcaatcca cttgctttga agacgtggtt ggaacgtctt ctttttccac
10321 gatgctcctc gtgggtgggg gtccatcttt gggaccactg tcggcagagg catcttgaac
10381 gatagccttt cctttatcgc aatgatggca tttgtaggtg ccaccttcct tttctactgt
10441 ccttttgatg aagtgacaga tagctgggca atggaatccg aggaggtttc ccgatattac
10501 cctttgttga aaagtctcaa tagccctttg gtcttctgag actgtatctt tgatattctt
10561 ggagtagacg agagtgtcgt gctccaccat gttgggcccg gcgcgccgaa ttcccgggga
10621 tccgtcgacc tgcagccaag ctttaagagg agtccac
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