LOCUS Exported 8743 bp ds-DNA circular SYN 11-JAN-2016
DEFINITION Agrobacterium binary vector for plant transformation, with
kanamycin-resistance genes.
ACCESSION AF234315
VERSION .
KEYWORDS pCAMBIA2300
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8743)
AUTHORS Cambia
TITLE Direct Submission
JOURNAL Exported Wednesday, September 14, 2016 from SnapGene Viewer 3.2.1
http://www.miaolingbio.com
COMMENT The GenBank record was corrected by inserting a G at position 2281.
FEATURES Location/Qualifiers
source 1..8743
/organism="synthetic DNA construct"
/lab_host="Plant Cells"
/mol_type="other DNA"
CDS 1219..1848
/codon_start=1
/product="stability protein from Pseudomonas plasmid pVS1"
/note="pVS1 StaA"
/translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
CDS 2277..3350
/codon_start=1
/product="replication protein from Pseudomonas plasmid
pVS1"
/note="pVS1 RepA"
/translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
LVMRYRNLIEGEASAGS"
rep_origin 3416..3610
/note="pVS1 oriV"
/note="origin of replication for the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
misc_feature 3954..4094
/note="bom"
/note="basis of mobility region from pBR322"
rep_origin complement(4280..4868)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(4955..5749)
/codon_start=1
/gene="aphA-3"
/product="aminoglycoside phosphotransferase"
/note="KanR"
/note="confers resistance to kanamycin"
/translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
misc_feature 6174..6198
/note="LB T-DNA repeat"
/note="left border repeat from nopaline C58 T-DNA"
polyA_signal 6276..6450
/note="CaMV poly(A) signal"
/note="cauliflower mosaic virus polyadenylation signal"
CDS complement(6507..7304)
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/note="NeoR/KanR"
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
promoter complement(7367..8044)
/note="CaMV 35S promoter (enhanced)"
/note="cauliflower mosaic virus 35S promoter with a
duplicated enhancer region"
promoter 8271..8301
/note="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind 8309..8325
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 8333..8349
/note="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
CDS 8345..8578
/codon_start=1
/gene="lacZ (truncated)"
/product="LacZ-alpha fragment of beta-galactosidase"
/note="lacZ-alpha"
/translation="MTMITNSSSVPGDPLESTCRHASLALAVVLQRRDWENPGVTQLNR
LAAHPPFASWRNSEEARTDRPSQQLRSLNGEC"
misc_feature 8359..8415
/note="MCS"
/note="pUC18/19 multiple cloning site"
primer_bind complement(8419..8435)
/note="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
misc_feature 8638..8662
/note="RB T-DNA repeat"
/note="right border repeat from nopaline C58 T-DNA"
ORIGIN
1 catgccaacc acagggttcc cctcgggatc aaagtacttt gatccaaccc ctccgctgct
61 atagtgcagt cggcttctga cgttcagtgc agccgtcttc tgaaaacgac atgtcgcaca
121 agtcctaagt tacgcgacag gctgccgccc tgcccttttc ctggcgtttt cttgtcgcgt
181 gttttagtcg cataaagtag aatacttgcg actagaaccg gagacattac gccatgaaca
241 agagcgccgc cgctggcctg ctgggctatg cccgcgtcag caccgacgac caggacttga
301 ccaaccaacg ggccgaactg cacgcggccg gctgcaccaa gctgttttcc gagaagatca
361 ccggcaccag gcgcgaccgc ccggagctgg ccaggatgct tgaccaccta cgccctggcg
421 acgttgtgac agtgaccagg ctagaccgcc tggcccgcag cacccgcgac ctactggaca
481 ttgccgagcg catccaggag gccggcgcgg gcctgcgtag cctggcagag ccgtgggccg
541 acaccaccac gccggccggc cgcatggtgt tgaccgtgtt cgccggcatt gccgagttcg
601 agcgttccct aatcatcgac cgcacccgga gcgggcgcga ggccgccaag gcccgaggcg
661 tgaagtttgg cccccgccct accctcaccc cggcacagat cgcgcacgcc cgcgagctga
721 tcgaccagga aggccgcacc gtgaaagagg cggctgcact gcttggcgtg catcgctcga
781 ccctgtaccg cgcacttgag cgcagcgagg aagtgacgcc caccgaggcc aggcggcgcg
841 gtgccttccg tgaggacgca ttgaccgagg ccgacgccct ggcggccgcc gagaatgaac
901 gccaagagga acaagcatga aaccgcacca ggacggccag gacgaaccgt ttttcattac
961 cgaagagatc gaggcggaga tgatcgcggc cgggtacgtg ttcgagccgc ccgcgcacgt
1021 ctcaaccgtg cggctgcatg aaatcctggc cggtttgtct gatgccaagc tggcggcctg
1081 gccggccagc ttggccgctg aagaaaccga gcgccgccgt ctaaaaaggt gatgtgtatt
1141 tgagtaaaac agcttgcgtc atgcggtcgc tgcgtatatg atgcgatgag taaataaaca
1201 aatacgcaag gggaacgcat gaaggttatc gctgtactta accagaaagg cgggtcaggc
1261 aagacgacca tcgcaaccca tctagcccgc gccctgcaac tcgccggggc cgatgttctg
1321 ttagtcgatt ccgatcccca gggcagtgcc cgcgattggg cggccgtgcg ggaagatcaa
1381 ccgctaaccg ttgtcggcat cgaccgcccg acgattgacc gcgacgtgaa ggccatcggc
1441 cggcgcgact tcgtagtgat cgacggagcg ccccaggcgg cggacttggc tgtgtccgcg
1501 atcaaggcag ccgacttcgt gctgattccg gtgcagccaa gcccttacga catatgggcc
1561 accgccgacc tggtggagct ggttaagcag cgcattgagg tcacggatgg aaggctacaa
1621 gcggcctttg tcgtgtcgcg ggcgatcaaa ggcacgcgca tcggcggtga ggttgccgag
1681 gcgctggccg ggtacgagct gcccattctt gagtcccgta tcacgcagcg cgtgagctac
1741 ccaggcactg ccgccgccgg cacaaccgtt cttgaatcag aacccgaggg cgacgctgcc
1801 cgcgaggtcc aggcgctggc cgctgaaatt aaatcaaaac tcatttgagt taatgaggta
1861 aagagaaaat gagcaaaagc acaaacacgc taagtgccgg ccgtccgagc gcacgcagca
1921 gcaaggctgc aacgttggcc agcctggcag acacgccagc catgaagcgg gtcaactttc
1981 agttgccggc ggaggatcac accaagctga agatgtacgc ggtacgccaa ggcaagacca
2041 ttaccgagct gctatctgaa tacatcgcgc agctaccaga gtaaatgagc aaatgaataa
2101 atgagtagat gaattttagc ggctaaagga ggcggcatgg aaaatcaaga acaaccaggc
2161 accgacgccg tggaatgccc catgtgtgga ggaacgggcg gttggccagg cgtaagcggc
2221 tgggttgtct gccggccctg caatggcact ggaaccccca agcccgagga atcggcgtga
2281 gcggtcgcaa accatccggc ccggtacaaa tcggcgcggc gctgggtgat gacctggtgg
2341 agaagttgaa ggccgcgcag gccgcccagc ggcaacgcat cgaggcagaa gcacgccccg
2401 gtgaatcgtg gcaagcggcc gctgatcgaa tccgcaaaga atcccggcaa ccgccggcag
2461 ccggtgcgcc gtcgattagg aagccgccca agggcgacga gcaaccagat tttttcgttc
2521 cgatgctcta tgacgtgggc acccgcgata gtcgcagcat catggacgtg gccgttttcc
2581 gtctgtcgaa gcgtgaccga cgagctggcg aggtgatccg ctacgagctt ccagacgggc
2641 acgtagaggt ttccgcaggg ccggccggca tggccagtgt gtgggattac gacctggtac
2701 tgatggcggt ttcccatcta accgaatcca tgaaccgata ccgggaaggg aagggagaca
2761 agcccggccg cgtgttccgt ccacacgttg cggacgtact caagttctgc cggcgagccg
2821 atggcggaaa gcagaaagac gacctggtag aaacctgcat tcggttaaac accacgcacg
2881 ttgccatgca gcgtacgaag aaggccaaga acggccgcct ggtgacggta tccgagggtg
2941 aagccttgat tagccgctac aagatcgtaa agagcgaaac cgggcggccg gagtacatcg
3001 agatcgagct agctgattgg atgtaccgcg agatcacaga aggcaagaac ccggacgtgc
3061 tgacggttca ccccgattac tttttgatcg atcccggcat cggccgtttt ctctaccgcc
3121 tggcacgccg cgccgcaggc aaggcagaag ccagatggtt gttcaagacg atctacgaac
3181 gcagtggcag cgccggagag ttcaagaagt tctgtttcac cgtgcgcaag ctgatcgggt
3241 caaatgacct gccggagtac gatttgaagg aggaggcggg gcaggctggc ccgatcctag
3301 tcatgcgcta ccgcaacctg atcgagggcg aagcatccgc cggttcctaa tgtacggagc
3361 agatgctagg gcaaattgcc ctagcagggg aaaaaggtcg aaaaggtctc tttcctgtgg
3421 atagcacgta cattgggaac ccaaagccgt acattgggaa ccggaacccg tacattggga
3481 acccaaagcc gtacattggg aaccggtcac acatgtaagt gactgatata aaagagaaaa
3541 aaggcgattt ttccgcctaa aactctttaa aacttattaa aactcttaaa acccgcctgg
3601 cctgtgcata actgtctggc cagcgcacag ccgaagagct gcaaaaagcg cctacccttc
3661 ggtcgctgcg ctccctacgc cccgccgctt cgcgtcggcc tatcgcggcc gctggccgct
3721 caaaaatggc tggcctacgg ccaggcaatc taccagggcg cggacaagcc gcgccgtcgc
3781 cactcgaccg ccggcgccca catcaaggca ccctgcctcg cgcgtttcgg tgatgacggt
3841 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc
3901 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc
3961 atgacccagt cacgtagcga tagcggagtg tatactggct taactatgcg gcatcagagc
4021 agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa
4081 aataccgcat caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
4141 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
4201 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
4261 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
4321 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
4381 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
4441 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
4501 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
4561 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
4621 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
4681 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
4741 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
4801 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
4861 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
4921 cacgttaagg gattttggtc atgcattcta ggtactaaaa caattcatcc agtaaaatat
4981 aatattttat tttctcccaa tcaggcttga tccccagtaa gtcaaaaaat agctcgacat
5041 actgttcttc cccgatatcc tccctgatcg accggacgca gaaggcaatg tcataccact
5101 tgtccgccct gccgcttctc ccaagatcaa taaagccact tactttgcca tctttcacaa
5161 agatgttgct gtctcccagg tcgccgtggg aaaagacaag ttcctcttcg ggcttttccg
5221 tctttaaaaa atcatacagc tcgcgcggat ctttaaatgg agtgtcttct tcccagtttt
5281 cgcaatccac atcggccaga tcgttattca gtaagtaatc caattcggct aagcggctgt
5341 ctaagctatt cgtataggga caatccgata tgtcgatgga gtgaaagagc ctgatgcact
5401 ccgcatacag ctcgataatc ttttcagggc tttgttcatc ttcatactct tccgagcaaa
5461 ggacgccatc ggcctcactc atgagcagat tgctccagcc atcatgccgt tcaaagtgca
5521 ggacctttgg aacaggcagc tttccttcca gccatagcat catgtccttt tcccgttcca
5581 catcataggt ggtcccttta taccggctgt ccgtcatttt taaatatagg ttttcatttt
5641 ctcccaccag cttatatacc ttagcaggag acattccttc cgtatctttt acgcagcggt
5701 atttttcgat cagttttttc aattccggtg atattctcat tttagccatt tattatttcc
5761 ttcctctttt ctacagtatt taaagatacc ccaagaagct aattataaca agacgaactc
5821 caattcactg ttccttgcat tctaaaacct taaataccag aaaacagctt tttcaaagtt
5881 gttttcaaag ttggcgtata acatagtatc gacggagccg attttgaaac cgcggtgatc
5941 acaggcagca acgctctgtc atcgttacaa tcaacatgct accctccgcg agatcatccg
6001 tgtttcaaac ccggcagctt agttgccgtt cttccgaata gcatcggtaa catgagcaaa
6061 gtctgccgcc ttacaacggc tctcccgctg acgccgtccc ggactgatgg gctgcctgta
6121 tcgagtggtg attttgtgcc gagctgccgg tcggggagct gttggctggc tggtggcagg
6181 atatattgtg gtgtaaacaa attgacgctt agacaactta ataacacatt gcggacgttt
6241 ttaatgtact gaattaacgc cgaattaatt cgggggatct ggattttagt actggatttt
6301 ggttttagga attagaaatt ttattgatag aagtatttta caaatacaaa tacatactaa
6361 gggtttctta tatgctcaac acatgagcga aaccctatag gaaccctaat tcccttatct
6421 gggaactact cacacattat tatggagaaa ctcgagcttg tcgatcgact ctagctagag
6481 gatcgatccg aaccccagag tcccgctcag aagaactcgt caagaaggcg atagaaggcg
6541 atgcgctgcg aatcgggagc ggcgataccg taaagcacga ggaagcggtc agcccattcg
6601 ccgccaagct cttcagcaat atcacgggta gccaacgcta tgtcctgata gcggtccgcc
6661 acacccagcc ggccacagtc gatgaatcca gaaaagcggc cattttccac catgatattc
6721 ggcaagcagg catcgccatg tgtcacgacg agatcctcgc cgtcgggcat gcgcgccttg
6781 agcctggcga acagttcggc tggcgcgagc ccctgatgct cttcgtccag atcatcctga
6841 tcgacaagac cggcttccat ccgagtacgt gctcgctcga tgcgatgttt cgcttggtgg
6901 tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc gcattgcatc agccatgatg
6961 gatactttct cggcaggagc aaggtgagat gacaggagat cctgccccgg cacttcgccc
7021 aatagcagcc agtcccttcc cgcttcagtg acaacgtcga gcacagctgc gcaaggaacg
7081 cccgtcgtgg ccagccacga tagccgcgct gcctcgtcct ggagttcatt cagggcaccg
7141 gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg ctgacagccg gaacacggcg
7201 gcatcagagc agccgattgt ctgttgtgcc cagtcatagc cgaatagcct ctccacccaa
7261 gcggccggag aacctgcgtg caatccatct tgttcaatcc ccatggtcga tcgacagatc
7321 tgcgaaagct cgagagagat agatttgtag agagagactg gtgatttcag cgtgtcctct
7381 ccaaatgaaa tgaacttcct tatatagagg aaggtcttgc gaaggatagt gggattgtgc
7441 gtcatccctt acgtcagtgg agatatcaca tcaatccact tgctttgaag acgtggttgg
7501 aacgtcttct ttttccacga tgctcctcgt gggtgggggt ccatctttgg gaccactgtc
7561 ggcagaggca tcttgaacga tagcctttcc tttatcgcaa tgatggcatt tgtaggtgcc
7621 accttccttt tctactgtcc ttttgatgaa gtgacagata gctgggcaat ggaatccgag
7681 gaggtttccc gatattaccc tttgttgaaa agtctcaata gccctttggt cttctgagac
7741 tgtatctttg atattcttgg agtagacgag agtgtcgtgc tccaccatgt tatcacatca
7801 atccacttgc tttgaagacg tggttggaac gtcttctttt tccacgatgc tcctcgtggg
7861 tgggggtcca tctttgggac cactgtcggc agaggcatct tgaacgatag cctttccttt
7921 atcgcaatga tggcatttgt aggtgccacc ttccttttct actgtccttt tgatgaagtg
7981 acagatagct gggcaatgga atccgaggag gtttcccgat attacccttt gttgaaaagt
8041 ctcaatagcc ctttggtctt ctgagactgt atctttgata ttcttggagt agacgagagt
8101 gtcgtgctcc accatgttgg caagctgctc tagccaatac gcaaaccgcc tctccccgcg
8161 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
8221 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
8281 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
8341 agctatgacc atgattacga attcgagctc ggtacccggg gatcctctag agtcgacctg
8401 caggcatgca agcttggcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
8461 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
8521 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgctagag
8581 cagcttgagc ttggatcaga ttgtcgtttc ccgccttcag tttaaactat cagtgtttga
8641 caggatatat tggcgggtaa acctaagaga aaagagcgtt tattagaata acggatattt
8701 aaaagggcgt gaaaaggttt atccgttcgt ccatttgtat gtg
//