LOCUS Exported 11633 bp ds-DNA circular SYN 14-SEP-2016
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS Untitled 7
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 11633)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Wednesday, September 14, 2016 from SnapGene Viewer 3.2.1
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..11633
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS join(2..16,207..2024)
/codon_start=1
/product="beta-glucuronidase"
/note="GUS"
/note="This version of the gusA gene has a 5' extension
with a catalase intron to ensure expression in plants but
not bacteria."
/translation="MVDLRNRRLVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWES
ALQESRAIAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGK
VWVNNQEVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGK
KKQSYFHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSV
ELRDADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELEVTAKSQTESDIYPLRVGIRS
VAVKGQQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYP
YAEEMLDWADEHGIVVIDETAAVGFQLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQA
IKELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDT
LAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKK
GIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
intron 17..206
/note="cat1 intron"
/note="castor bean catalase intron, modified"
CDS 2031..2048
/codon_start=1
/product="6xHis affinity tag"
/note="6xHis"
/translation="HHHHHH"
terminator 2083..2335
/note="NOS terminator"
/note="nopaline synthase terminator and poly(A) signal"
misc_feature 2357..2381
/note="RB T-DNA repeat"
/note="right border repeat from nopaline C58 T-DNA"
CDS 3681..4310
/codon_start=1
/product="stability protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/note="pVS1 StaA"
/translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
CDS 4744..5811
/codon_start=1
/product="replication protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/note="pVS1 RepA"
/translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
MRYRNLIEGEASAGS"
rep_origin 5877..6071
/note="pVS1 oriV"
/note="origin of replication for the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
misc_feature 6415..6555
/note="bom"
/note="basis of mobility region from pBR322"
rep_origin complement(6741..7329)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(7416..8210)
/codon_start=1
/gene="aphA-3"
/product="aminoglycoside phosphotransferase"
/note="KanR"
/note="confers resistance to kanamycin"
/translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
misc_feature 8635..8659
/note="LB T-DNA repeat"
/note="left border repeat from nopaline C58 T-DNA"
polyA_signal 8737..8911
/note="CaMV poly(A) signal"
/note="cauliflower mosaic virus polyadenylation signal"
CDS complement(8968..9765)
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/note="NeoR/KanR"
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
promoter complement(9828..10505)
/note="CaMV 35S promoter (enhanced)"
/note="cauliflower mosaic virus 35S promoter with a
duplicated enhancer region"
protein_bind 10696..10717
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 10732..10762
/note="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind 10770..10786
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 10794..10810
/note="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
CDS 10806..11039
/codon_start=1
/gene="lacZ fragment"
/product="LacZ-alpha fragment of beta-galactosidase"
/note="lacZ-alpha"
/translation="MTMITNSSSVPGDPLESTCRHASLALAVVLQRRDWENPGVTQLNR
LAAHPPFASWRNSEEARTDRPSQQLRSLNGEC"
misc_feature 10820..10876
/note="MCS"
/note="pUC18/19 multiple cloning site"
primer_bind complement(10880..10896)
/note="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
promoter 11273..11618
/note="CaMV 35S promoter"
/note="strong constitutive promoter from cauliflower mosaic
virus"
ORIGIN
1 catggtagat ctgagggtaa atttctagtt tttctccttc attttcttgg ttaggaccct
61 tttctctttt tatttttttg agctttgatc tttctttaaa ctgatctatt ttttaattga
121 ttggttatgg tgtaaatatt acatagcttt aactgataat ctgattactt tatttcgtgt
181 gtctatgatg atgatgatag ttacagaacc gacgactcgt ccgtcctgta gaaaccccaa
241 cccgtgaaat caaaaaactc gacggcctgt gggcattcag tctggatcgc gaaaactgtg
301 gaattgatca gcgttggtgg gaaagcgcgt tacaagaaag ccgggcaatt gctgtgccag
361 gcagttttaa cgatcagttc gccgatgcag atattcgtaa ttatgcgggc aacgtctggt
421 atcagcgcga agtctttata ccgaaaggtt gggcaggcca gcgtatcgtg ctgcgtttcg
481 atgcggtcac tcattacggc aaagtgtggg tcaataatca ggaagtgatg gagcatcagg
541 gcggctatac gccatttgaa gccgatgtca cgccgtatgt tattgccggg aaaagtgtac
601 gtatcaccgt ttgtgtgaac aacgaactga actggcagac tatcccgccg ggaatggtga
661 ttaccgacga aaacggcaag aaaaagcagt cttacttcca tgatttcttt aactatgccg
721 gaatccatcg cagcgtaatg ctctacacca cgccgaacac ctgggtggac gatatcaccg
781 tggtgacgca tgtcgcgcaa gactgtaacc acgcgtctgt tgactggcag gtggtggcca
841 atggtgatgt cagcgttgaa ctgcgtgatg cggatcaaca ggtggttgca actggacaag
901 gcactagcgg gactttgcaa gtggtgaatc cgcacctctg gcaaccgggt gaaggttatc
961 tctatgaact cgaagtcaca gccaaaagcc agacagagtc tgatatctac ccgcttcgcg
1021 tcggcatccg gtcagtggca gtgaagggcc aacagttcct gattaaccac aaaccgttct
1081 actttactgg ctttggtcgt catgaagatg cggacttacg tggcaaagga ttcgataacg
1141 tgctgatggt gcacgaccac gcattaatgg actggattgg ggccaactcc taccgtacct
1201 cgcattaccc ttacgctgaa gagatgctcg actgggcaga tgaacatggc atcgtggtga
1261 ttgatgaaac tgctgctgtc ggctttcagc tgtctttagg cattggtttc gaagcgggca
1321 acaagccgaa agaactgtac agcgaagagg cagtcaacgg ggaaactcag caagcgcact
1381 tacaggcgat taaagagctg atagcgcgtg acaaaaacca cccaagcgtg gtgatgtgga
1441 gtattgccaa cgaaccggat acccgtccgc aaggtgcacg ggaatatttc gcgccactgg
1501 cggaagcaac gcgtaaactc gacccgacgc gtccgatcac ctgcgtcaat gtaatgttct
1561 gcgacgctca caccgatacc atcagcgatc tctttgatgt gctgtgcctg aaccgttatt
1621 acggatggta tgtccaaagc ggcgatttgg aaacggcaga gaaggtactg gaaaaagaac
1681 ttctggcctg gcaggagaaa ctgcatcagc cgattatcat caccgaatac ggcgtggata
1741 cgttagccgg gctgcactca atgtacaccg acatgtggag tgaagagtat cagtgtgcat
1801 ggctggatat gtatcaccgc gtctttgatc gcgtcagcgc cgtcgtcggt gaacaggtat
1861 ggaatttcgc cgattttgcg acctcgcaag gcatattgcg cgttggcggt aacaagaaag
1921 ggatcttcac tcgcgaccgc aaaccgaagt cggcggcttt tctgctgcaa aaacgctgga
1981 ctggcatgaa cttcggtgaa aaaccgcagc agggaggcaa acaagctagc caccaccacc
2041 accaccacgt gtgaattaca ggtgaccagc tcgaatttcc ccgatcgttc aaacatttgg
2101 caataaagtt tcttaagatt gaatcctgtt gccggtcttg cgatgattat catataattt
2161 ctgttgaatt acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga
2221 tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata
2281 tagcgcgcaa actaggataa attatcgcgc gcggtgtcat ctatgttact agatcgggaa
2341 ttaaactatc agtgtttgac aggatatatt ggcgggtaaa cctaagagaa aagagcgttt
2401 attagaataa cggatattta aaagggcgtg aaaaggttta tccgttcgtc catttgtatg
2461 tgcatgccaa ccacagggtt cccctcggga tcaaagtact ttgatccaac ccctccgctg
2521 ctatagtgca gtcggcttct gacgttcagt gcagccgtct tctgaaaacg acatgtcgca
2581 caagtcctaa gttacgcgac aggctgccgc cctgcccttt tcctggcgtt ttcttgtcgc
2641 gtgttttagt cgcataaagt agaatacttg cgactagaac cggagacatt acgccatgaa
2701 caagagcgcc gccgctggcc tgctgggcta tgcccgcgtc agcaccgacg accaggactt
2761 gaccaaccaa cgggccgaac tgcacgcggc cggctgcacc aagctgtttt ccgagaagat
2821 caccggcacc aggcgcgacc gcccggagct ggccaggatg cttgaccacc tacgccctgg
2881 cgacgttgtg acagtgacca ggctagaccg cctggcccgc agcacccgcg acctactgga
2941 cattgccgag cgcatccagg aggccggcgc gggcctgcgt agcctggcag agccgtgggc
3001 cgacaccacc acgccggccg gccgcatggt gttgaccgtg ttcgccggca ttgccgagtt
3061 cgagcgttcc ctaatcatcg accgcacccg gagcgggcgc gaggccgcca aggcccgagg
3121 cgtgaagttt ggcccccgcc ctaccctcac cccggcacag atcgcgcacg cccgcgagct
3181 gatcgaccag gaaggccgca ccgtgaaaga ggcggctgca ctgcttggcg tgcatcgctc
3241 gaccctgtac cgcgcacttg agcgcagcga ggaagtgacg cccaccgagg ccaggcggcg
3301 cggtgccttc cgtgaggacg cattgaccga ggccgacgcc ctggcggccg ccgagaatga
3361 acgccaagag gaacaagcat gaaaccgcac caggacggcc aggacgaacc gtttttcatt
3421 accgaagaga tcgaggcgga gatgatcgcg gccgggtacg tgttcgagcc gcccgcgcac
3481 gtctcaaccg tgcggctgca tgaaatcctg gccggtttgt ctgatgccaa gctggcggcc
3541 tggccggcca gcttggccgc tgaagaaacc gagcgccgcc gtctaaaaag gtgatgtgta
3601 tttgagtaaa acagcttgcg tcatgcggtc gctgcgtata tgatgcgatg agtaaataaa
3661 caaatacgca aggggaacgc atgaaggtta tcgctgtact taaccagaaa ggcgggtcag
3721 gcaagacgac catcgcaacc catctagccc gcgccctgca actcgccggg gccgatgttc
3781 tgttagtcga ttccgatccc cagggcagtg cccgcgattg ggcggccgtg cgggaagatc
3841 aaccgctaac cgttgtcggc atcgaccgcc cgacgattga ccgcgacgtg aaggccatcg
3901 gccggcgcga cttcgtagtg atcgacggag cgccccaggc ggcggacttg gctgtgtccg
3961 cgatcaaggc agccgacttc gtgctgattc cggtgcagcc aagcccttac gacatatggg
4021 ccaccgccga cctggtggag ctggttaagc agcgcattga ggtcacggat ggaaggctac
4081 aagcggcctt tgtcgtgtcg cgggcgatca aaggcacgcg catcggcggt gaggttgccg
4141 aggcgctggc cgggtacgag ctgcccattc ttgagtcccg tatcacgcag cgcgtgagct
4201 acccaggcac tgccgccgcc ggcacaaccg ttcttgaatc agaacccgag ggcgacgctg
4261 cccgcgaggt ccaggcgctg gccgctgaaa ttaaatcaaa actcatttga gttaatgagg
4321 taaagagaaa atgagcaaaa gcacaaacac gctaagtgcc ggccgtccga gcgcacgcag
4381 cagcaaggct gcaacgttgg ccagcctggc agacacgcca gccatgaagc gggtcaactt
4441 tcagttgccg gcggaggatc acaccaagct gaagatgtac gcggtacgcc aaggcaagac
4501 cattaccgag ctgctatctg aatacatcgc gcagctacca gagtaaatga gcaaatgaat
4561 aaatgagtag atgaatttta gcggctaaag gaggcggcat ggaaaatcaa gaacaaccag
4621 gcaccgacgc cgtggaatgc cccatgtgtg gaggaacggg cggttggcca ggcgtaagcg
4681 gctgggttgt ctgccggccc tgcaatggca ctggaacccc caagcccgag gaatcggcgt
4741 gacggtcgca aaccatccgg cccggtacaa atcggcgcgg cgctgggtga tgacctggtg
4801 gagaagttga aggccgcgca ggccgcccag cggcaacgca tcgaggcaga agcacgcccc
4861 ggtgaatcgt ggcaagcggc cgctgatcga atccgcaaag aatcccggca accgccggca
4921 gccggtgcgc cgtcgattag gaagccgccc aagggcgacg agcaaccaga ttttttcgtt
4981 ccgatgctct atgacgtggg cacccgcgat agtcgcagca tcatggacgt ggccgttttc
5041 cgtctgtcga agcgtgaccg acgagctggc gaggtgatcc gctacgagct tccagacggg
5101 cacgtagagg tttccgcagg gccggccggc atggccagtg tgtgggatta cgacctggta
5161 ctgatggcgg tttcccatct aaccgaatcc atgaaccgat accgggaagg gaagggagac
5221 aagcccggcc gcgtgttccg tccacacgtt gcggacgtac tcaagttctg ccggcgagcc
5281 gatggcggaa agcagaaaga cgacctggta gaaacctgca ttcggttaaa caccacgcac
5341 gttgccatgc agcgtacgaa gaaggccaag aacggccgcc tggtgacggt atccgagggt
5401 gaagccttga ttagccgcta caagatcgta aagagcgaaa ccgggcggcc ggagtacatc
5461 gagatcgagc tagctgattg gatgtaccgc gagatcacag aaggcaagaa cccggacgtg
5521 ctgacggttc accccgatta ctttttgatc gatcccggca tcggccgttt tctctaccgc
5581 ctggcacgcc gcgccgcagg caaggcagaa gccagatggt tgttcaagac gatctacgaa
5641 cgcagtggca gcgccggaga gttcaagaag ttctgtttca ccgtgcgcaa gctgatcggg
5701 tcaaatgacc tgccggagta cgatttgaag gaggaggcgg ggcaggctgg cccgatccta
5761 gtcatgcgct accgcaacct gatcgagggc gaagcatccg ccggttccta atgtacggag
5821 cagatgctag ggcaaattgc cctagcaggg gaaaaaggtc gaaaaggtct ctttcctgtg
5881 gatagcacgt acattgggaa cccaaagccg tacattggga accggaaccc gtacattggg
5941 aacccaaagc cgtacattgg gaaccggtca cacatgtaag tgactgatat aaaagagaaa
6001 aaaggcgatt tttccgccta aaactcttta aaacttatta aaactcttaa aacccgcctg
6061 gcctgtgcat aactgtctgg ccagcgcaca gccgaagagc tgcaaaaagc gcctaccctt
6121 cggtcgctgc gctccctacg ccccgccgct tcgcgtcggc ctatcgcggc cgctggccgc
6181 tcaaaaatgg ctggcctacg gccaggcaat ctaccagggc gcggacaagc cgcgccgtcg
6241 ccactcgacc gccggcgccc acatcaaggc accctgcctc gcgcgtttcg gtgatgacgg
6301 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc
6361 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc
6421 catgacccag tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag
6481 cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga
6541 aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
6601 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
6661 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
6721 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
6781 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
6841 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
6901 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
6961 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
7021 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
7081 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
7141 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
7201 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
7261 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
7321 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
7381 tcacgttaag ggattttggt catgcattct aggtactaaa acaattcatc cagtaaaata
7441 taatatttta ttttctccca atcaggcttg atccccagta agtcaaaaaa tagctcgaca
7501 tactgttctt ccccgatatc ctccctgatc gaccggacgc agaaggcaat gtcataccac
7561 ttgtccgccc tgccgcttct cccaagatca ataaagccac ttactttgcc atctttcaca
7621 aagatgttgc tgtctcccag gtcgccgtgg gaaaagacaa gttcctcttc gggcttttcc
7681 gtctttaaaa aatcatacag ctcgcgcgga tctttaaatg gagtgtcttc ttcccagttt
7741 tcgcaatcca catcggccag atcgttattc agtaagtaat ccaattcggc taagcggctg
7801 tctaagctat tcgtataggg acaatccgat atgtcgatgg agtgaaagag cctgatgcac
7861 tccgcataca gctcgataat cttttcaggg ctttgttcat cttcatactc ttccgagcaa
7921 aggacgccat cggcctcact catgagcaga ttgctccagc catcatgccg ttcaaagtgc
7981 aggacctttg gaacaggcag ctttccttcc agccatagca tcatgtcctt ttcccgttcc
8041 acatcatagg tggtcccttt ataccggctg tccgtcattt ttaaatatag gttttcattt
8101 tctcccacca gcttatatac cttagcagga gacattcctt ccgtatcttt tacgcagcgg
8161 tatttttcga tcagtttttt caattccggt gatattctca ttttagccat ttattatttc
8221 cttcctcttt tctacagtat ttaaagatac cccaagaagc taattataac aagacgaact
8281 ccaattcact gttccttgca ttctaaaacc ttaaatacca gaaaacagct ttttcaaagt
8341 tgttttcaaa gttggcgtat aacatagtat cgacggagcc gattttgaaa ccgcggtgat
8401 cacaggcagc aacgctctgt catcgttaca atcaacatgc taccctccgc gagatcatcc
8461 gtgtttcaaa cccggcagct tagttgccgt tcttccgaat agcatcggta acatgagcaa
8521 agtctgccgc cttacaacgg ctctcccgct gacgccgtcc cggactgatg ggctgcctgt
8581 atcgagtggt gattttgtgc cgagctgccg gtcggggagc tgttggctgg ctggtggcag
8641 gatatattgt ggtgtaaaca aattgacgct tagacaactt aataacacat tgcggacgtt
8701 tttaatgtac tgaattaacg ccgaattaat tcgggggatc tggattttag tactggattt
8761 tggttttagg aattagaaat tttattgata gaagtatttt acaaatacaa atacatacta
8821 agggtttctt atatgctcaa cacatgagcg aaaccctata ggaaccctaa ttcccttatc
8881 tgggaactac tcacacatta ttatggagaa actcgagctt gtcgatcgac tctagctaga
8941 ggatcgatcc gaaccccaga gtcccgctca gaagaactcg tcaagaaggc gatagaaggc
9001 gatgcgctgc gaatcgggag cggcgatacc gtaaagcacg aggaagcggt cagcccattc
9061 gccgccaagc tcttcagcaa tatcacgggt agccaacgct atgtcctgat agcggtccgc
9121 cacacccagc cggccacagt cgatgaatcc agaaaagcgg ccattttcca ccatgatatt
9181 cggcaagcag gcatcgccat gtgtcacgac gagatcctcg ccgtcgggca tgcgcgcctt
9241 gagcctggcg aacagttcgg ctggcgcgag cccctgatgc tcttcgtcca gatcatcctg
9301 atcgacaaga ccggcttcca tccgagtacg tgctcgctcg atgcgatgtt tcgcttggtg
9361 gtcgaatggg caggtagccg gatcaagcgt atgcagccgc cgcattgcat cagccatgat
9421 ggatactttc tcggcaggag caaggtgaga tgacaggaga tcctgccccg gcacttcgcc
9481 caatagcagc cagtcccttc ccgcttcagt gacaacgtcg agcacagctg cgcaaggaac
9541 gcccgtcgtg gccagccacg atagccgcgc tgcctcgtcc tggagttcat tcagggcacc
9601 ggacaggtcg gtcttgacaa aaagaaccgg gcgcccctgc gctgacagcc ggaacacggc
9661 ggcatcagag cagccgattg tctgttgtgc ccagtcatag ccgaatagcc tctccaccca
9721 agcggccgga gaacctgcgt gcaatccatc ttgttcaatc cccatggtcg atcgacagat
9781 ctgcgaaagc tcgagagaga tagatttgta gagagagact ggtgatttca gcgtgtcctc
9841 tccaaatgaa atgaacttcc ttatatagag gaaggtcttg cgaaggatag tgggattgtg
9901 cgtcatccct tacgtcagtg gagatatcac atcaatccac ttgctttgaa gacgtggttg
9961 gaacgtcttc tttttccacg atgctcctcg tgggtggggg tccatctttg ggaccactgt
10021 cggcagaggc atcttgaacg atagcctttc ctttatcgca atgatggcat ttgtaggtgc
10081 caccttcctt ttctactgtc cttttgatga agtgacagat agctgggcaa tggaatccga
10141 ggaggtttcc cgatattacc ctttgttgaa aagtctcaat agccctttgg tcttctgaga
10201 ctgtatcttt gatattcttg gagtagacga gagtgtcgtg ctccaccatg ttatcacatc
10261 aatccacttg ctttgaagac gtggttggaa cgtcttcttt ttccacgatg ctcctcgtgg
10321 gtgggggtcc atctttggga ccactgtcgg cagaggcatc ttgaacgata gcctttcctt
10381 tatcgcaatg atggcatttg taggtgccac cttccttttc tactgtcctt ttgatgaagt
10441 gacagatagc tgggcaatgg aatccgagga ggtttcccga tattaccctt tgttgaaaag
10501 tctcaatagc cctttggtct tctgagactg tatctttgat attcttggag tagacgagag
10561 tgtcgtgctc caccatgttg gcaagctgct ctagccaata cgcaaaccgc ctctccccgc
10621 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
10681 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
10741 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
10801 cagctatgac catgattacg aattcgagct cggtacccgg ggatcctcta gagtcgacct
10861 gcaggcatgc aagcttggca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
10921 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
10981 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgctaga
11041 gcagcttgag cttggatcag attgtcgttt cccgccttca gtttagcttc atggagtcaa
11101 agattcaaat agaggaccta acagaactcg ccgtaaagac tggcgaacag ttcatacaga
11161 gtctcttacg actcaatgac aagaagaaaa tcttcgtcaa catggtggag cacgacacac
11221 ttgtctactc caaaaatatc aaagatacag tctcagaaga ccaaagggca attgagactt
11281 ttcaacaaag ggtaatatcc ggaaacctcc tcggattcca ttgcccagct atctgtcact
11341 ttattgtgaa gatagtggaa aaggaaggtg gctcctacaa atgccatcat tgcgataaag
11401 gaaaggccat cgttgaagat gcctctgccg acagtggtcc caaagatgga cccccaccca
11461 cgaggagcat cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa gtggattgat
11521 gtgatatctc cactgacgta agggatgacg cacaatccca ctatccttcg caagaccctt
11581 cctctatata aggaagttca tttcatttgg agagaacacg ggggactctt gac
//