LOCUS Exported 4410 bp ds-DNA circular SYN 04-JAN-2018
DEFINITION synthetic circular DNA
FEATURES Location/Qualifiers
source 1..4410
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 863..1208
/label=CaMV 35S promoter
/note="strong constitutive promoter from cauliflower mosaic
virus"
misc_feature 1217..1300
/label=MCS
CDS 1304..1333
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
CDS 1334..1852
/codon_start=1
/product="N-terminal fragment of mVenus for use in
bimolecular fluorescence complementation (BiFC) (Kodama and
Hu, 2010)"
/label=VN173
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIE"
terminator 1872..2124
/label=NOS terminator
/note="nopaline synthase terminator and poly(A) signal"
promoter 2623..2727
/gene="bla"
/label=AmpR promoter
CDS 2728..3588
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 3759..4347
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
61 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
121 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
181 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
241 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
301 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc caagcttgca
361 tgcctgcagg tccccagatt agccttttca atttcagaaa gaatgctaac ccacagatgg
421 ttagagaggc ttacgcagca ggtctcatca agacgatcta cccgagcaat aatctccagg
481 aaatcaaata ccttcccaag aaggttaaag atgcagtcaa aagattcagg actaactgca
541 tcaagaacac agagaaagat atatttctca agatcagaag tactattcca gtatggacga
601 ttcaaggctt gcttcacaaa ccaaggcaag taatagagat tggagtctct aaaaaggtag
661 ttcccactga atcaaaggcc atggagtcaa agattcaaat agaggaccta acagaactcg
721 ccgtaaagac tggcgaacag ttcatacaga gtctcttacg actcaatgac aagaagaaaa
781 tcttcgtcaa catggtggag cacgacacac ttgtctactc caaaaatatc aaagatacag
841 tctcagaaga ccaaagggca attgagactt ttcaacaaag ggtaatatcc ggaaacctcc
901 tcggattcca ttgcccagct atctgtcact ttattgtgaa gatagtggaa aaggaaggtg
961 gctcctacaa atgccatcat tgcgataaag gaaaggccat cgttgaagat gcctctgccg
1021 acagtggtcc caaagatgga cccccaccca cgaggagcat cgtggaaaaa gaagacgttc
1081 caaccacgtc ttcaaagcaa gtggattgat gtgatatctc cactgacgta agggatgacg
1141 cacaatccca ctatccttcg caagaccctt cctctatata aggaagttca tttcatttgg
1201 agagaacacg ggggactcta gagttaaccg ggctcaggcc tggcgcgcca ctagtggatc
1261 catcgatagt actgtcgacc tcgagggtac cgctcccggg atggagcaaa agttgatttc
1321 tgaggaggat cttatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
1381 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg
1441 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
1501 gccctggccc accctcgtga ccaccttcgg ctacggcctg cagtgcttcg cccgctaccc
1561 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
1621 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
1681 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
1741 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga
1801 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg agtaggagct
1861 cgaatttccc cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg
1921 ccggtcttgc gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta
1981 acatgtaatg catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat
2041 acatttaata cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg
2101 cggtgtcatc tatgttacta gatcgggaat tcactggccg tcgttttaca acgtcgtgac
2161 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
2221 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
2281 ggcgaatggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc
2341 atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac
2401 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
2461 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
2521 cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
2581 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
2641 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
2701 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
2761 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
2821 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
2881 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
2941 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
3001 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
3061 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
3121 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
3181 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
3241 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
3301 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
3361 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
3421 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
3481 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
3541 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
3601 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
3661 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
3721 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
3781 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
3841 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
3901 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
3961 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
4021 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
4081 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
4141 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
4201 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
4261 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
4321 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
4381 gccttttgct ggccttttgc tcacatgttc
//