LOCUS Exported 4113 bp ds-DNA circular SYN 04-JAN-2018
DEFINITION synthetic circular DNA
FEATURES Location/Qualifiers
source 1..4113
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 863..1208
/label=CaMV 35S promoter
/note="strong constitutive promoter from cauliflower mosaic
virus"
CDS 1226..1477
/codon_start=1
/product="C-terminal fragment of mVenus for use in
bimolecular fluorescence complementation (BiFC) (Kodama and
Hu, 2010)"
/label=VC155
/translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
YLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
CDS 1478..1504
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
misc_feature 1505..1564
/label=MCS
terminator 1575..1827
/label=NOS terminator
/note="nopaline synthase terminator and poly(A) signal"
promoter 2326..2430
/gene="bla"
/label=AmpR promoter
CDS 2431..3291
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 3462..4050
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
61 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
121 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
181 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
241 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
301 gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc caagcttgca
361 tgcctgcagg tccccagatt agccttttca atttcagaaa gaatgctaac ccacagatgg
421 ttagagaggc ttacgcagca ggtctcatca agacgatcta cccgagcaat aatctccagg
481 aaatcaaata ccttcccaag aaggttaaag atgcagtcaa aagattcagg actaactgca
541 tcaagaacac agagaaagat atatttctca agatcagaag tactattcca gtatggacga
601 ttcaaggctt gcttcacaaa ccaaggcaag taatagagat tggagtctct aaaaaggtag
661 ttcccactga atcaaaggcc atggagtcaa agattcaaat agaggaccta acagaactcg
721 ccgtaaagac tggcgaacag ttcatacaga gtctcttacg actcaatgac aagaagaaaa
781 tcttcgtcaa catggtggag cacgacacac ttgtctactc caaaaatatc aaagatacag
841 tctcagaaga ccaaagggca attgagactt ttcaacaaag ggtaatatcc ggaaacctcc
901 tcggattcca ttgcccagct atctgtcact ttattgtgaa gatagtggaa aaggaaggtg
961 gctcctacaa atgccatcat tgcgataaag gaaaggccat cgttgaagat gcctctgccg
1021 acagtggtcc caaagatgga cccccaccca cgaggagcat cgtggaaaaa gaagacgttc
1081 caaccacgtc ttcaaagcaa gtggattgat gtgatatctc cactgacgta agggatgacg
1141 cacaatccca ctatccttcg caagaccctt cctctatata aggaagttca tttcatttgg
1201 agagaacacg ggggactcta gaatggacaa gcagaagaac ggcatcaagg tgaacttcaa
1261 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
1321 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagct accagtccaa
1381 gctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
1441 cgccgggatc actctcggca tggacgagct gtacaagtac ccatacgatg ttccagatta
1501 cgctgggccc aggcctacta gtggatccgt cgacctcgag ggtaccgctc ccgggtagga
1561 gctcgaattt ccccgatcgt tcaaacattt ggcaataaag tttcttaaga ttgaatcctg
1621 ttgccggtct tgcgatgatt atcatataat ttctgttgaa ttacgttaag catgtaataa
1681 ttaacatgta atgcatgacg ttatttatga gatgggtttt tatgattaga gtcccgcaat
1741 tatacattta atacgcgata gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc
1801 gcgcggtgtc atctatgtta ctagatcggg aattcactgg ccgtcgtttt acaacgtcgt
1861 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
1921 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
1981 aatggcgaat ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
2041 cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga
2101 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
2161 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
2221 aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
2281 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
2341 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
2401 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
2461 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
2521 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
2581 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
2641 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
2701 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
2761 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
2821 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
2881 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
2941 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
3001 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
3061 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
3121 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
3181 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
3241 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
3301 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
3361 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
3421 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
3481 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
3541 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
3601 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
3661 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
3721 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
3781 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
3841 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
3901 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
3961 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
4021 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
4081 ctggcctttt gctggccttt tgctcacatg ttc
//