LOCUS Exported 19008 bp ds-DNA circular SYN 21-DEC-2017
DEFINITION synthetic circular DNA
FEATURES Location/Qualifiers
source 1..19008
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_feature 113..137
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
protein_bind 295..316
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 331..361
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 369..385
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 393..409
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 485..509
/gene="mutant version of attB"
/label=attB1
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
protein_bind 578..677
/gene="mutant version of attL"
/label=attL2
/bound_moiety="LR Clonase(TM)"
/note="recombination site for the Gateway(R) LR reaction"
protein_bind 683..707
/gene="mutant version of attB"
/label=attB2
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
misc_RNA complement(719..794)
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
protein_bind complement(1203..1302)
/gene="mutant version of attL"
/label=attL1
/bound_moiety="LR Clonase(TM)"
/note="recombination site for the Gateway(R) LR reaction"
primer_bind 1323..1339
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
promoter 2111..2156
/label=minimal CaMV 35S promoter
/note="minimal 35S promoter from cauliflower mosaic virus"
5'UTR 2167..2267
/label=OsADH 5'-UTR
/note="translational enhancer from the 5'-UTR of the Oryza
sativa alcohol dehydrogenase gene (Sugio et al., 2008)"
CDS 2277..2297
/codon_start=1
/product="nuclear localization signal of SV40 large T
antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 2322..6425
/codon_start=1
/product="catalytically dead mutant of the Cas9
endonuclease from the Streptococcus pyogenes Type II
CRISPR/Cas system"
/label=dCas9
/note="RNA-guided DNA-binding protein that lacks
endonuclease activity due to the D10A mutation in the RuvC
catalytic domain and the H840A mutation in the HNH
catalytic domain"
/translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
DATLIHQSITGLYETRIDLSQLGGD"
CDS 6447..6467
/codon_start=1
/product="nuclear localization signal of SV40 large T
antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 6660..6725
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
CDS 7359..7379
/codon_start=1
/product="nuclear localization signal of SV40 large T
antigen"
/label=SV40 NLS
/translation="PKKKRKV"
promoter 9657..10002
/label=CaMV 35S promoter
/note="strong constitutive promoter from cauliflower mosaic
virus"
CDS 10017..11042
/codon_start=1
/gene="aph(4)-Ia"
/product="aminoglycoside phosphotransferase from E. coli"
/label=HygR
/note="confers resistance to hygromycin"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
K"
protein_bind complement(12194..12318)
/gene="mutant version of attR"
/label=attR2
/bound_moiety="LR Clonase(TM)"
/note="recombination site for the Gateway(R) LR reaction"
misc_feature 12589..12613
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA"
CDS 13134..13925
/codon_start=1
/gene="aadA"
/product="aminoglycoside adenylyltransferase (Murphy,
1985)"
/label=SmR
/note="confers resistance to spectinomycin and
streptomycin"
/translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
rep_origin 14171..14759
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
misc_feature 14945..15085
/label=bom
/note="basis of mobility region from pBR322"
rep_origin 15429..15623
/label=pVS1 oriV
/note="origin of replication for the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
CDS complement(15689..16762)
/codon_start=1
/product="replication protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/label=pVS1 RepA
/translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
LVMRYRNLIEGEASAGS"
CDS complement(17191..17820)
/codon_start=1
/product="stability protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/label=pVS1 StaA
/translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
1 gatcccgagg ggaaccctgt ggttggcatg cacatacaaa tggacgaacg gataaacctt
61 ttcacgccct tttaaatatc cgattattct aataaacgct cttttctctt aggtttaccc
121 gccaatatat cctgtcaaac actgatagtt taaactgaag gcgggaaacg acaatctgat
181 ccaagctcaa gctccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
241 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
301 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
361 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgaca tgattacgaa
421 tttcaggtga ctgatagtga cctgttcgtt gcaacaaatt gatgagcaat gcttttttat
481 aatgccaact ttgtacaaaa aagcaggcgg cgcgtcctgc aggataggga taacagggta
541 atttaattaa ggtaccgagc tcggatccac tagtgatcaa ataatgattt tattttgact
601 gatagtgacc tgttcgttgc aacaaattga tgagcaatta ttttttataa tgccaacttt
661 gtacaagaaa gctgggttaa tgccaacttt gtacaagaaa gctgggtcta gaaaaaaagc
721 accgactcgg tgccactttt tcaagttgat aacggactag ccttatttta acttgctatt
781 tctagctcta aaacggtgga tctccatgga cgcgcaatca ctacttcgac tctagctgta
841 tataaactca gcttcgtttt cttatctaag cgatgtggga cttttgaaga ttgttttcaa
901 cttaaatggg cctatataag aaatactatt gttctttccc atataaatgg gcctgcttct
961 cttctttcag attcccaggg gccttttgaa gattatcttc atatcttaag aatgaagatg
1021 ttttattcaa tcaaattctt gaaggttcga tgcctaatca ttctaatcct gggacaaact
1081 atgaaacaag atacaaaaac tccgaatgga aagttaaaaa gaagaaaacg aaagctacgg
1141 ttcaagaaaa tgtaagctga taaacaaaaa aaaactgtat gaacgaagaa gaagaaaaaa
1201 agagcctgct tttttgtaca aagttggcat tataaaaaag cattgctcat caatttgttg
1261 caacgaacag gtcactatca gtcaaaataa aatcattatt tgatcgaatt cccgcggccg
1321 ctcgaggtcg acggtatcga taagcttggc gcgccattac cctgttatcc ctatcctgca
1381 ggattaacac ccaacttttc tatccaagct tgccaacatg gtggagcacg acactctcgt
1441 ctactccaag aatatcaaag atacagtctc agaagaccaa agggctattg agacttttca
1501 acaaagggta atatcgggaa acctcctcgg attccattgc ccagctatct gtcacttcat
1561 caaaaggaca gtagaaaagg aaggtggcac ctacaaatgc catcattgcg ataaaggaaa
1621 ggctatcgtt caagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag
1681 gagcatcgtg gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga
1741 acatggtgga gcacgacact ctcgtctact ccaagaatat caaagataca gtctcagaag
1801 accaaagggc tattgagact tttcaacaaa gggtaatatc gggaaacctc ctcggattcc
1861 attgcccagc tatctgtcac ttcatcaaaa ggacagtaga aaaggaaggt ggcacctaca
1921 aatgccatca ttgcgataaa ggaaaggcta tcgttcaaga tgcctctgcc gacagtggtc
1981 ccaaagatgg acccccaccc acgaggagca tcgtggaaaa agaagacgtt ccaaccacgt
2041 cttcaaagca agtggattga tgtgatatct ccactgacgt aagggatgac gcacaatccc
2101 actatccttc gcaagaccct tcctctatat aaggaagttc atttcatttg gagaggccgg
2161 tctagagaat tccaagcaac gaactgcgag tgattcaaga aaaaagaaaa cctgagcttt
2221 cgatctctac ggagtggttt cttgttcttt gaaaaagagg gggattacat atggctccta
2281 agaagaagcg gaaggttggt attcacgggg tgcctgcggc tatggacaag aagtactcga
2341 tcgggctggc catcggaaca aattctgtag gctgggctgt aataaccgat gagtacaagg
2401 tgccctctaa aaaatttaag gtccttggca atacggatag acattccata aagaagaatc
2461 ttatcggtgc gctgctcttt gacagcggcg agaccgcgga ggcgacccgg ttgaaacgca
2521 ccgcgagacg ccgttacaca aggcgtaaga atagaatctg ttatctccag gagatattct
2581 ctaatgaaat ggcgaaggta gacgattcct tctttcaccg tctggaggaa agttttctcg
2641 ttgaggaaga taagaaacat gaaagacacc cgatcttcgg aaacattgtc gacgaggttg
2701 cttatcatga aaagtaccct accatctacc atcttagaaa gaaacttgtt gacagcacgg
2761 ataaggctga tctcaggctg atatacctgg ctctggcaca tatgattaag ttcagagggc
2821 atttccttat cgaaggcgac ctgaatccag ataattcaga tgtagacaag ctcttcattc
2881 aacttgtgca gacttataat cagctcttcg aagaaaatcc aataaacgcg tcgggtgtag
2941 acgcaaaggc catactgtcc gctaggcttt ctaagtcacg tagacttgag aatctcattg
3001 cccaactccc cggcgagaag aagaacggct tgtttggaaa tctgatagcg ctgtccctgg
3061 gtcttacacc aaatttcaag agtaatttcg atttggcaga agatgctaag ttgcagctca
3121 gtaaagacac ctacgatgac gatcttgata atttgttggc tcagattggc gatcagtatg
3181 cagatctttt cttggccgct aagaatttgt ctgatgcaat tctgcttagc gacattttga
3241 gggttaatac agaaatcacc aaggcaccct tgtcggcgtc aatgataaag aggtatgatg
3301 agcaccacca agacctgacg ctcctcaagg ctcttgttcg gcagcaattg ccggagaagt
3361 acaaagagat cttcttcgac cagtctaaga acggatatgc gggctacata gacggtggag
3421 cgagtcagga ggaattctac aagttcataa agcccattct cgagaagatg gatggtacgg
3481 aagaactgct tgtgaaactt aacagagaag atcttttgcg gaagcagaga actttcgaca
3541 acggaagtat accacaccag atacatctcg gagagcttca tgctattctc agaagacaag
3601 aggatttcta ccctttcttg aaggataaca gagaaaagat agagaagatc ctcacgttta
3661 ggatccctta ctacgtaggt cctcttgctc gcggcaatag taggttcgcc tggatgaccc
3721 gcaagtctga agaaactatc accccttgga atttcgaaga ggttgtagac aaaggtgctt
3781 cagcacagag tttcattgag aggatgacca acttcgacaa gaacctcccc aacgaaaagg
3841 tcctgcctaa gcacagcctc ctctacgaat actttactgt ctataatgag cttacaaaag
3901 ttaagtacgt gacagaggga atgcggaagc ccgcattcct ttccggagaa caaaagaagg
3961 cgatcgtgga tcttctcttc aagacgaacc gcaaggtgac ggttaaacag ttgaaggaag
4021 attacttcaa gaagatagaa tgttttgata gcgtggaaat cagcggcgtc gaagataggt
4081 tcaacgcttc cctgggaacg taccacgatc tcctcaagat tatcaaagat aaggactttc
4141 ttgataacga agagaatgag gacatcttgg aagacattgt tctgacgctc accctgttcg
4201 aagatcgcga gatgattgag gaacgcttga agacctacgc acacctgttc gatgacaagg
4261 ttatgaagca acttaaacgg cgccggtata cgggctgggg acggctttcg cggaagctga
4321 taaatggaat ccgtgacaag cagtctggca agacaatact cgacttcttg aagtcggatg
4381 gttttgccaa tagaaatttt atgcaactca ttcatgatga ctcgcttact tttaaggagg
4441 acatccagaa ggcccaggta tcaggacagg gtgactcttt gcacgaacac atcgcgaacc
4501 tggcgggctc ccccgcgatt aagaagggaa ttttgcagac tgtcaaggtg gtcgatgaac
4561 tcgtgaaggt tatgggacgt cataagccgg aaaatattgt gattgagatg gctcgcgaga
4621 atcaaacaac acagaagggc caaaagaaca gtagagaacg catgaagcgc atcgaagagg
4681 gcatcaaaga gctgggcagt cagatcctta aagaacatcc agtcgagaat acacagcttc
4741 agaacgaaaa gctgtacctt tattaccttc aaaatgggcg tgatatgtat gtggatcagg
4801 aactcgatat caataggctg agtgactatg atgtcgacgc tatcgtcccg caaagtttcc
4861 tcaaggacga cagtatagac aacaaagttc tcacacggtc agataagaat cgcggcaaga
4921 gcgataatgt accgtcggag gaggtagtca agaagatgaa gaattactgg cgccagttgc
4981 tcaacgccaa gctcatcact cagaggaaat ttgacaacct tacgaaagcc gagcggggcg
5041 gactctctga actggacaag gccggtttca taaagcgcca gctcgttgag acacgtcaaa
5101 ttactaagca cgtcgctcaa atattggatt cccgcatgaa tactaagtac gatgagaatg
5161 ataagctcat acgtgaagtt aaggtcatta ctctcaagtc caagcttgta tcggacttcc
5221 gtaaggactt ccaattctac aaggtccggg aaatcaataa ttatcaccat gcccatgacg
5281 cttatctgaa cgcggtcgtg ggcacggcac tcattaagaa atacccaaaa cttgagtcag
5341 aatttgttta cggggactat aaagtttatg acgtgcggaa gatgatagcg aagtcggaac
5401 aagagatagg aaaggcgact gcaaagtact ttttttactc caacataatg aatttcttta
5461 agaccgaaat aacccttgca aacggtgaaa tcagaaagcg gcctctgatt gaaacaaatg
5521 gcgagacggg cgagatcgtc tgggacaagg ggagggactt cgcaacggtt cgcaaggtcc
5581 ttagcatgcc gcaagtaaat atagttaaga agacggaagt tcagaccggc ggctttagta
5641 aagaaagcat acttcctaaa aggaattccg acaaactgat agcgcgcaag aaggactggg
5701 atccaaagaa gtatggagga tttgactccc caaccgttgc ttatagcgtg ttggtagtag
5761 ccaaggtgga aaagggtaag tctaagaaat tgaagtcggt gaaggagttg ttggggataa
5821 ctataatgga gcggagttcg ttcgagaaga acccaattga ctttctcgaa gccaaaggct
5881 acaaggaggt caagaaggac ctgattatta agttgccaaa gtactcgctc ttcgaactcg
5941 agaacgggag aaagcgtatg ctggcgtcgg cgggcgagct gcagaaagga aacgagctgg
6001 ctttgccatc gaaatacgta aatttcctgt acctcgcctc acattatgag aagcttaaag
6061 ggtctccaga agacaatgaa cagaagcagc tgtttgttga acagcacaag cactacttgg
6121 acgagattat agaacaaatc tccgagttct ctaaacgggt tatccttgca gacgccaatt
6181 tggataaggt cctctcggct tataataagc atagagataa gccaatccgg gagcaggctg
6241 aaaatatcat acacctcttt acgttgacta atttgggtgc gccagcggca ttcaagtact
6301 tcgatacaac aatcgatcgt aagcgctaca caagcactaa ggaagtcctg gacgcgacgc
6361 tgatacacca gtccattact ggactgtatg aaaccagaat agatcttagc cagctcggcg
6421 gtgatggagg agggcctagg gctgatccta agaagaagag gaaggttgga ggagggccag
6481 gagcagagta tgttagagcg ttgtttgatt ttaacggtaa cgatgaagag gatttaccct
6541 ttaagaaagg cgacattctc aggattaggg ataaacctga agagcaatgg tggaatgctg
6601 aggatagtga aggcaaacga ggaatgattt tagtgccgta tgtggagaaa tattcgggtg
6661 actacaaaga tcatgatggt gattacaaag accatgacat cgactacaag gatgatgatg
6721 ataagtcagg gatgacagat gctgaatatg tcagaatcca cgaaaagttg gacatttaca
6781 cgtttaagaa gcagttcttc aacaacaaga aatctgtttc gcataggtgc tatgtgcttt
6841 tcgaactaaa acgtcgtgga gaaagacggg cttgcttttg gggttacgcg gttaacaaac
6901 cacaatcagg tactgaacga ggaatacacg ctgaaatctt ttctatccga aaggttgagg
6961 aatatctacg tgacaatcct ggacagttca ctatcaattg gtattctagc tggtcaccat
7021 gtgcagattg tgctgagaag attctcgaat ggtacaatca agagcttaga ggcaatggac
7081 atacattgaa aatatgggca tgcaagctct actacgaaaa gaatgccaga aaccaaattg
7141 ggctttggaa cttgagggat aatggagttg ggcttaatgt catggtttct gagcactatc
7201 aatgttgtcg gaagatcttc atacaaagtt cccataacca gttgaatgag aacagatggt
7261 tagagaaaac ccttaaaaga gccgagaaga gaagatccga actgagcatt atgatacagg
7321 tcaaaattct gcataccact aagagtccag ctgtaggtcc taagaagaaa cgtaaagtag
7381 ggccctgatc caggcctccc agctttcgtc cgtatcatcg gtttcgacaa cgttcgtcaa
7441 gttcaatgca tcagtttcat tgcccacaca ccagaatcct actaagtttg agtattatgg
7501 cattggaaaa gctgttttct tctatcattt gttctgcttg taatttactg tgttctttca
7561 gtttttgttt tcggacatca aaatgcaaat ggatggataa gagttaataa atgatatggt
7621 ccttttgttc attctcaaat tattattatc tgttgttttt actttaatgg gttgaattta
7681 agtaagaaag gaactaacag tgtgatatta aggtgcaatg ttagacatat aaaacagtct
7741 ttcacctctc tttggttatg tcttgaattg gtttgtttct tcacttatct gtgtaatcaa
7801 gtttactatg agtctatgat caagtaatta tgcaatcaag ttaagtacag tataggcttg
7861 agctccctag gcccctaggt aattcttcgg acccaagaat gctaagccaa gaggagctgt
7921 tatcgccgtc ctcctgcttg tttctctctt tttgttgctg tttcttcatt agcgtggaca
7981 aagttttcaa ccggcctatc tgttatcatt ttcttctatt caaagactgt aatacctatt
8041 gctacctgtg gttctcactt gtgattttgg acacatatgt tcggtttatt caaatttaat
8101 cagatgcctg atgagggtac cagaaaaaat acgtgttctg gttgtttttg agttgcgatt
8161 attctatgaa atgaataaca tcgaagttat catcccagta ttttcgcatg aatgttcttt
8221 tcttctgtct tgtgcatcag tgatctagtg catgggagtt tgtattgtga tgttcgacat
8281 cacgtaactt ccactttgcc tttgctgttc gatattttaa tgacatgtca cacacacttc
8341 tgatactttt ctttcttggc tattgtgcca gcatgatgca agatgcatca cagcatcaga
8401 tatattctca tcgtcaggct ttagcagcac acgagcacgc tttgccgctt aaaagttgta
8461 cggcgcagct tagacatccc ctgtagaagt gataatcttt tcacttttcc ttaaacaaat
8521 tgagagggga aatggaacca tgtggatcag agaagctttt gtttctttac acaagaatat
8581 ttggtacagt gggggtccta tgttcgtggg ttcgtggctt ggctgcctgt cttcaaccaa
8641 gtgttttcag ttcaacatgt tagcgtgtag aaagagcaca attctgttta tctccaaggt
8701 aaaatgtggc attctgttaa agaacatgat cctgccaatt ttttaagttt caatggaaga
8761 ggaatgtaaa gctttctatg gtttgtgtac acaacacagt ggaagaggag tgcaagcttt
8821 ctatggtttg tgtgcgcgtt gtgtgtcagc acttcaattt tgttagaaaa tgaaagaaaa
8881 aaaaggatga tcatgcttat agtaaatcac tctttttcct cgccttctgt acgttttgac
8941 ttgacaagat tttaaaatct gtacatgacc tttgttttaa aattacttta tgtatttcca
9001 tctttcaagt tatgcagatg tcatcacaaa ttgttacacc aatcaccagg ctggctgttt
9061 atatattatc agaccaggct atatagagta tactatacta actgttcata ttatctggaa
9121 atcttgcttg ctacttgagc ggtaaaaggg tatagatatg agggtcccca gattagcctt
9181 ttcaatttca gaaagaatgc taacccacag atggttagag aggcttacgc agcaggtctc
9241 atcaagacga tctacccgag caataatctc caggaaatca aataccttcc caagaaggtt
9301 aaagatgcag tcaaaagatt caggactaac tgcatcaaga acacagagaa agatatattt
9361 ctcaagatca gaagtactat tccagtatgg acgattcaag gcttgcttca caaaccaagg
9421 caagtaatag agattggagt ctctaaaaag gtagttccca ctgaatcaaa ggccatggag
9481 tcaaagattc aaatagagga cctaacagaa ctcgccgtaa agactggcga acagttcata
9541 cagagtctct tacgactcaa tgacaagaag aaaatcttcg tcaacatggt ggagcacgac
9601 acacttgtct actccaaaaa tatcaaagat acagtctcag aagaccaaag ggcaattgag
9661 acttttcaac aaagggtaat atccggaaac ctcctcggat tccattgccc agctatctgt
9721 cactttattg tgaagatagt ggaaaaggaa ggtggctcct acaaatgcca tcattgcgat
9781 aaaggaaagg ccatcgttga agatgcctct gccgacagtg gtcccaaaga tggaccccca
9841 cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat
9901 tgatgtgata tctccactga cgtaagggat gacgcacaat cccactatcc ttcgcaagac
9961 ccttcctcta tataaggaag ttcatttcat ttggagagaa cacgggggac tctagaatga
10021 aaaagcctga actcaccgcg acgtctgtcg agaagtttct gatcgaaaag ttcgacagcg
10081 tctccgacct gatgcagctc tcggagggcg aagaatctcg tgctttcagc ttcgatgtag
10141 gagggcgtgg atatgtcctg cgggtaaata gctgcgccga tggtttctac aaagatcgtt
10201 atgtttatcg gcactttgca tcggccgcgc tcccgattcc ggaagtgctt gacattgggg
10261 agtttagcga gagcctgacc tattgcatct cccgccgttc acagggtgtc acgttgcaag
10321 acctgcctga aaccgaactg cccgctgttc tacaaccggt cgcggaggct atggatgcga
10381 tcgctgcggc cgatcttagc cagacgagcg ggttcggccc attcggaccg caaggaatcg
10441 gtcaatacac tacatggcgt gatttcatat gcgcgattgc tgatccccat gtgtatcact
10501 ggcaaactgt gatggacgac accgtcagtg cgtccgtcgc gcaggctctc gatgagctga
10561 tgctttgggc cgaggactgc cccgaagtcc ggcacctcgt gcacgcggat ttcggctcca
10621 acaatgtcct gacggacaat ggccgcataa cagcggtcat tgactggagc gaggcgatgt
10681 tcggggattc ccaatacgag gtcgccaaca tcttcttctg gaggccgtgg ttggcttgta
10741 tggagcagca gacgcgctac ttcgagcgga ggcatccgga gcttgcagga tcgccacgac
10801 tccgggcgta tatgctccgc attggtcttg accaactcta tcagagcttg gttgacggca
10861 atttcgatga tgcagcttgg gcgcagggtc gatgcgacgc aatcgtccga tccggagccg
10921 ggactgtcgg gcgtacacaa atcgcccgca gaagcgcggc cgtctggacc gatggctgtg
10981 tagaagtact cgccgatagt ggaaaccgac gccccagcac tcgtccgagg gcaaagaaat
11041 aggagctctg ggctaatcta aaacgattta tctgtggctt caagtgtatc gatcacttat
11101 gtgaggtgta attactggtg tttttggtgt gctctggttc ctttcaagtg tgttgttgcc
11161 gctcgaacta ctccgctatg taaaacggta aaacctgttg tctcattatg aaagtgaact
11221 atattatgtt ctactactac tctacttagt caattttctt caccttgatt agtgtaaata
11281 tgaatcttat attcttatgt cttaagaaat tagcacatgt gaagcctcca agtgcatatt
11341 ttctcgatcg cgagacgcac aatgcgtgag aaattcagct ggttatactc aaatatatta
11401 atatatctag cagcagctca tggagattca ggaaacttgg catccctaat ccctaccatt
11461 tccattcttc cgagattgac agttcaatac aagtacagta atctcctggt aagtttctta
11521 ttaacttgac atgtagtagt aataatttgt acgtagcata gatacataga cacaaaaatg
11581 tcctccccat tgagctagcc gattggagcc gaacaccgca ggaatgaatt tacataatct
11641 gcaaagaata aatggaatgt gcctccacag gaaaaccagc ggcagtgtgg cgttttcaag
11701 agcagccgta agtcgaagcc tattctgaat cgtagaaatc actggggcat ggtgtaatta
11761 catccgactc caacatctgt accaccctgt gcattgtagg ccgctcctct ggcaaagaac
11821 ttacacattg tttagcaaga gaaagtagag catccaaggt ctcaatctgc actccctcac
11881 aatatggatc gacaatttcc ctctcccgat tctcaccgac caggaaattc aactgccaca
11941 gagcaagtag attatttcaa gaatacatta aatcaattga aggcatacgt aattcatatc
12001 agaaaactgt ggatatgaaa tggaatgaca taaaggtcat acatacccat ccaacaatgt
12061 tcaatccctt ttcaataaat gatgcatcag taggtcgttt tccgcttagt atttcaagta
12121 gcaaaactcc aaaactgtag acgtcagtct tttcggtggc tctgccactt tgcatatact
12181 cctgcaggtc gaccatagtg actggatatg ttgtgtttta cagtattatg tagtctgttt
12241 tttatgcaaa atctaattta atatattgat atttatatca ttttacgttt ctcgttcagc
12301 tttcttgtac aaagtggtga tatcccgcgg aaatacgtag aattcgggaa gcttggcact
12361 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
12421 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
12481 ttcccaacag ttgcgcagcc tgaatggcga atgctagagc aattcggcgt taattcagta
12541 cattaaaaac gtccgcaatg tgttattaag ttgtctaagc gtcaatttgt ttacaccaca
12601 atatatcctg ccaccagcca gccaacagct ccccgaccgg cagctcggca caaaatcacc
12661 actcgataca ggcagcccat cagtccggga cggcgtcagc gggagagccg ttgtaaggcg
12721 gcagactttg ctcatgttac cgatgctatt cggaagaacg gcaactaagc tgccgggttt
12781 gaaacacgga tgatctcgcg gagggtagca tgttgattgt aacgatgaca gagcgttgct
12841 gcctgtgatc aattcgggca cgaacccagt ggacataagc ctgttcggtt cgtaagctgt
12901 aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg
12961 gtaacggcgc agtggcggtt ttcatggctt gttatgactg tttttttggg gtacagtcta
13021 tgcctcgggc atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga
13081 gcagcaacga tgttacgcag cagggcagtc gccctaaaac aaagttaaac atcatggggg
13141 aagcggtgat cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc
13201 atctcgaacc gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga
13261 agccacacag tgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc
13321 ggcgagcttt gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc
13381 tccgcgctgt agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag
13441 ctaagcgcga actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg
13501 agccagccac gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg
13561 ttgccttggt aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat
13621 ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg
13681 agcgaaatgt agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg
13741 cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg
13801 tcatacttga agctagacag gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg
13861 cagatcagtt ggaagaattt gtccactacg tgaaaggcga gatcaccaag gtagtcggca
13921 aataatgtct agctagaaat tcgttcaagc cgacgccgct tcgcggcgcg gcttaactca
13981 agcgttagat gcactaagca cataattgct cacagccaaa ctatcaggtc aagtctgctt
14041 ttattatttt taagcgtgca taataagccc tacacaaatt gggagatata tcatgcatga
14101 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
14161 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
14221 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
14281 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag
14341 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
14401 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
14461 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
14521 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
14581 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
14641 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
14701 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
14761 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
14821 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
14881 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
14941 agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt
15001 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc
15061 gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg
15121 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
15181 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagggtg ccttgatgtg
15241 ggcgccggcg gtcgagtggc gacggcgcgg cttgtccgcg ccctggtaga ttgcctggcc
15301 gtaggccagc catttttgag cggccagcgg ccgcgatagg ccgacgcgaa gcggcggggc
15361 gtagggagcg cagcgaccga agggtaggcg ctttttgcag ctcttcggct gtgcgctggc
15421 cagacagtta tgcacaggcc aggcgggttt taagagtttt aataagtttt aaagagtttt
15481 aggcggaaaa atcgcctttt ttctctttta tatcagtcac ttacatgtgt gaccggttcc
15541 caatgtacgg ctttgggttc ccaatgtacg ggttccggtt cccaatgtac ggctttgggt
15601 tcccaatgta cgtgctatcc acaggaaaga gaccttttcg acctttttcc cctgctaggg
15661 caatttgccc tagcatctgc tccgtacatt aggaaccggc ggatgcttcg ccctcgatca
15721 ggttgcggta gcgcatgact aggatcgggc cagcctgccc cgcctcctcc ttcaaatcgt
15781 actccggcag gtcatttgac ccgatcagct tgcgcacggt gaaacagaac ttcttgaact
15841 ctccggcgct gccactgcgt tcgtagatcg tcttgaacaa ccatctggct tctgccttgc
15901 ctgcggcgcg gcgtgccagg cggtagagaa aacggccgat gccgggatcg atcaaaaagt
15961 aatcggggtg aaccgtcagc acgtccgggt tcttgccttc tgtgatctcg cggtacatcc
16021 aatcagctag ctcgatctcg atgtactccg gccgcccggt ttcgctcttt acgatcttgt
16081 agcggctaat caaggcttca ccctcggata ccgtcaccag gcggccgttc ttggccttct
16141 tcgtacgctg catggcaacg tgcgtggtgt ttaaccgaat gcaggtttct accaggtcgt
16201 ctttctgctt tccgccatcg gctcgccggc agaacttgag tacgtccgca acgtgtggac
16261 ggaacacgcg gccgggcttg tctcccttcc cttcccggta tcggttcatg gattcggtta
16321 gatgggaaac cgccatcagt accaggtcgt aatcccacac actggccatg ccggccggcc
16381 ctgcggaaac ctctacgtgc ccgtctggaa gctcgtagcg gatcacctcg ccagctcgtc
16441 ggtcacgctt cgacagacgg aaaacggcca cgtccatgat gctgcgacta tcgcgggtgc
16501 ccacgtcata gagcatcgga acgaaaaaat ctggttgctc gtcgcccttg ggcggcttcc
16561 taatcgacgg cgcaccggct gccggcggtt gccgggattc tttgcggatt cgatcagcgg
16621 ccgcttgcca cgattcaccg gggcgtgctt ctgcctcgat gcgttgccgc tgggcggcct
16681 gcgcggcctt caacttctcc accaggtcat cacccagcgc cgcgccgatt tgtaccgggc
16741 cggatggttt gcgaccgctc acgccgattc ctcgggcttg ggggttccag tgccattgca
16801 gggccggcag acaacccagc cgcttacgcc tggccaaccg cccgttcctc cacacatggg
16861 gcattccacg gcgtcggtgc ctggttgttc ttgattttcc atgccgcctc ctttagccgc
16921 taaaattcat ctactcattt attcatttgc tcatttactc tggtagctgc gcgatgtatt
16981 cagatagcag ctcggtaatg gtcttgcctt ggcgtaccgc gtacatcttc agcttggtgt
17041 gatcctccgc cggcaactga aagttgaccc gcttcatggc tggcgtgtct gccaggctgg
17101 ccaacgttgc agccttgctg ctgcgtgcgc tcggacggcc ggcacttagc gtgtttgtgc
17161 ttttgctcat tttctcttta cctcattaac tcaaatgagt tttgatttaa tttcagcggc
17221 cagcgcctgg acctcgcggg cagcgtcgcc ctcgggttct gattcaagaa cggttgtgcc
17281 ggcggcggca gtgcctgggt agctcacgcg ctgcgtgata cgggactcaa gaatgggcag
17341 ctcgtacccg gccagcgcct cggcaacctc accgccgatg cgcgtgcctt tgatcgcccg
17401 cgacacgaca aaggccgctt gtagccttcc atccgtgacc tcaatgcgct gcttaaccag
17461 ctccaccagg tcggcggtgg cccatatgtc gtaagggctt ggctgcaccg gaatcagcac
17521 gaagtcggct gccttgatcg cggacacagc caagtccgcc gcctggggcg ctccgtcgat
17581 cactacgaag tcgcgccggc cgatggcctt cacgtcgcgg tcaatcgtcg ggcggtcgat
17641 gccgacaacg gttagcggtt gatcttcccg cacggccgcc caatcgcggg cactgccctg
17701 gggatcggaa tcgactaaca gaacatcggc cccggcgagt tgcagggcgc gggctagatg
17761 ggttgcgatg gtcgtcttgc ctgacccgcc tttctggtta agtacagcga taaccttcat
17821 gcgttcccct tgcgtatttg tttatttact catcgcatca tatacgcagc gaccgcatga
17881 cgcaagctgt tttactcaaa tacacatcac ctttttagac ggcggcgctc ggtttcttca
17941 gcggccaagc tggccggcca ggccgccagc ttggcatcag acaaaccggc caggatttca
18001 tgcagccgca cggttgagac gtgcgcgggc ggctcgaaca cgtacccggc cgcgatcatc
18061 tccgcctcga tctcttcggt aatgaaaaac ggttcgtcct ggccgtcctg gtgcggtttc
18121 atgcttgttc ctcttggcgt tcattctcgg cggccgccag ggcgtcggcc tcggtcaatg
18181 cgtcctcacg gaaggcaccg cgccgcctgg cctcggtggg cgtcacttcc tcgctgcgct
18241 caagtgcgcg gtacagggtc gagcgatgca cgccaagcag tgcagccgcc tctttcacgg
18301 tgcggccttc ctggtcgatc agctcgcggg cgtgcgcgat ctgtgccggg gtgagggtag
18361 ggcgggggcc aaacttcacg cctcgggcct tggcggcctc gcgcccgctc cgggtgcggt
18421 cgatgattag ggaacgctcg aactcggcaa tgccggcgaa cacggtcaac accatgcggc
18481 cggccggcgt ggtggtgtcg gcccacggct ctgccaggct acgcaggccc gcgccggcct
18541 cctggatgcg ctcggcaatg tccagtaggt cgcgggtgct gcgggccagg cggtctagcc
18601 tggtcactgt cacaacgtcg ccagggcgta ggtggtcaag catcctggcc agctccgggc
18661 ggtcgcgcct ggtgccggtg atcttctcgg aaaacagctt ggtgcagccg gccgcgtgca
18721 gttcggcccg ttggttggtc aagtcctggt cgtcggtgct gacgcgggca tagcccagca
18781 ggccagcggc ggcgctcttg ttcatggcgt aatgtctccg gttctagtcg caagtattct
18841 actttatgcg actaaaacac gcgacaagaa aacgccagga aaagggcagg gcggcagcct
18901 gtcgcgtaac ttaggacttg tgcgacatgt cgttttcaga agacggctgc actgaacgtc
18961 agaagccgac tgcactatag cagcggaggg gttggatcaa agtacttt
//