LOCUS Exported 12634 bp ds-DNA circular SYN 11-OCT-2017
DEFINITION synthetic circular DNA
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 12634)
TITLE Direct Submission
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..12634
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 299..644
/label=CaMV 35S promoter
/note="strong constitutive promoter from cauliflower mosaic
virus"
CDS 664..729
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
CDS 736..756
/codon_start=1
/product="nuclear localization signal of SV40 large T
antigen"
/label=SV40 NLS
/translation="PKKKRKV"
misc_feature 781..3438
/label=NgAgo
CDS 3439..3486
/codon_start=1
/product="bipartite nuclear localization signal from
nucleoplasmin"
/label=nucleoplasmin NLS
/translation="KRPAATKKAGQAKKKK"
terminator 3527..3779
/label=NOS terminator
/note="nopaline synthase terminator and poly(A) signal"
misc_feature 3801..3825
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
CDS 5125..5754
/codon_start=1
/product="stability protein from Pseudomonas plasmid pVS1"
/label=pVS1 StaA
/translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
CDS 6188..7255
/codon_start=1
/product="replication protein from Pseudomonas plasmid
pVS1"
/label=pVS1 RepA
/translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
MRYRNLIEGEASAGS"
misc_feature 7859..7999
/label=bom
/note="basis of mobility region from pBR322"
rep_origin complement(8185..8773)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(8860..9654)
/codon_start=1
/gene="aphA-3"
/product="aminoglycoside phosphotransferase"
/label=KanR
/note="confers resistance to kanamycin"
/translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
misc_feature 10079..10103
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA"
polyA_signal 10181..10355
/label=CaMV poly(A) signal
/note="cauliflower mosaic virus polyadenylation signal"
CDS complement(10395..11420)
/codon_start=1
/product="hygromycin B phosphotransferase"
/label=HygR
/note="confers resistance to hygromycin"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
K"
promoter complement(11488..12165)
/label=CaMV 35S promoter (enhanced)
/note="cauliflower mosaic virus 35S promoter with a
duplicated enhancer region"
primer_bind 12454..12470
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
CDS 12466..65
/codon_start=1
/gene="lacZ fragment"
/product="LacZ-alpha fragment of beta-galactosidase"
/label=lacZ-alpha
/translation="MTMITNSSSVPGDPLESTCRHASLALAVVLQRRDWENPGVTQLNR
LAAHPPFASWRNSEEARTDRPSQQLRSLNGEC"
misc_feature 12480..12536
/label=MCS
/note="pUC18/19 multiple cloning site"
primer_bind complement(12540..12556)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
ORIGIN
1 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
61 gctagagcag cttgagcttg gatcagattg tcgtttcccg ccttcagttt agcttcatgg
121 agtcaaagat tcaaatagag gacctaacag aactcgccgt aaagactggc gaacagttca
181 tacagagtct cttacgactc aatgacaaga agaaaatctt cgtcaacatg gtggagcacg
241 acacacttgt ctactccaaa aatatcaaag atacagtctc agaagaccaa agggcaattg
301 agacttttca acaaagggta atatccggaa acctcctcgg attccattgc ccagctatct
361 gtcactttat tgtgaagata gtggaaaagg aaggtggctc ctacaaatgc catcattgcg
421 ataaaggaaa ggccatcgtt gaagatgcct ctgccgacag tggtcccaaa gatggacccc
481 cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca aagcaagtgg
541 attgatgtga tatctccact gacgtaaggg atgacgcaca atcccactat ccttcgcaag
601 acccttcctc tatataagga agttcatttc atttggagag aacacggggg actcttgacc
661 atggactata aggaccacga cggagactac aaggatcatg atattgatta caaagacgat
721 gacgataaga tggccccaaa gaagaagcgg aaggtcggta tccacggagt cccagcagcc
781 actgttattg atttggattc tactactact gctgatgaat tgacttctgg acatacttat
841 gatatttctg ttactttgac tggagtttat gataatactg atgaacaaca tcctagaatg
901 tctttggctt ttgaacaaga taatggagaa agaagatata ttactttgtg gaagaatact
961 actcctaagg atgtttttac ttatgattat gctactggat ctacttatat ttttactaat
1021 attgattatg aagttaagga tggatatgaa aatttgactg ctacttatca aactactgtt
1081 gaaaatgcta ctgctcaaga agttggaact actgatgaag atgaaacttt tgctggagga
1141 gaacctttgg atcatcattt ggatgatgct ttgaatgaaa ctcctgatga tgctgaaact
1201 gaatctgatt ctggacatgt tatgacttct tttgcttcta gagatcaatt gcctgaatgg
1261 actttgcata cttatacttt gactgctact gatggagcta agactgatac tgaatatgct
1321 agaagaactt tggcttatac tgttagacaa gaattgtata ctgatcatga tgctgctcct
1381 gttgctactg atggattgat gttgttgact cctgaacctt tgggagaaac tcctttggat
1441 ttggattgtg gagttagagt tgaagctgat gaaactagaa ctttggatta tactactgct
1501 aaggatagat tgttggctag agaattggtt gaagaaggat tgaagagatc tttgtgggat
1561 gattatttgg ttagaggaat tgatgaagtt ttgtctaagg aacctgtttt gacttgtgat
1621 gaatttgatt tgcatgaaag atatgatttg tctgttgaag ttggacattc tggaagagct
1681 tatttgcata ttaattttag acatagattt gttcctaagt tgactttggc tgatattgat
1741 gatgataata tttatcctgg attgagagtt aagactactt atagacctag aagaggacat
1801 attgtttggg gattgagaga tgaatgtgct actgattctt tgaatacttt gggaaatcaa
1861 tctgttgttg cttatcatag aaataatcaa actcctatta atactgattt gttggatgct
1921 attgaagctg ctgatagaag agttgttgaa actagaagac aaggacatgg agatgatgct
1981 gtttcttttc ctcaagaatt gttggctgtt gaacctaata ctcatcaaat taagcaattt
2041 gcttctgatg gatttcatca acaagctaga tctaagacta gattgtctgc ttctagatgt
2101 tctgaaaagg ctcaagcttt tgctgaaaga ttggatcctg ttagattgaa tggatctact
2161 gttgaatttt cttctgaatt ttttactgga aataatgaac aacaattgag attgttgtat
2221 gaaaatggag aatctgtttt gacttttaga gatggagcta gaggagctca tcctgatgaa
2281 actttttcta agggaattgt taatcctcct gaatcttttg aagttgctgt tgttttgcct
2341 gaacaacaag ctgatacttg taaggctcaa tgggatacta tggctgattt gttgaatcaa
2401 gctggagctc ctcctactag atctgaaact gttcaatatg atgctttttc ttctcctgaa
2461 tctatttctt tgaatgttgc tggagctatt gatccttctg aagttgatgc tgcttttgtt
2521 gttttgcctc ctgatcaaga aggatttgct gatttggctt ctcctactga aacttatgat
2581 gaattgaaga aggctttggc taatatggga atttattctc aaatggctta ttttgataga
2641 tttagagatg ctaagatttt ttatactaga aatgttgctt tgggattgtt ggctgctgct
2701 ggaggagttg cttttactac tgaacatgct atgcctggag atgctgatat gtttattgga
2761 attgatgttt ctagatctta tcctgaagat ggagcttctg gacaaattaa tattgctgct
2821 actgctactg ctgtttataa ggatggaact attttgggac attcttctac tagacctcaa
2881 ttgggagaaa agttgcaatc tactgatgtt agagatatta tgaagaatgc tattttggga
2941 tatcaacaag ttactggaga atctcctact catattgtta ttcatagaga tggatttatg
3001 aatgaagatt tggatcctgc tactgaattt ttgaatgaac aaggagttga atatgatatt
3061 gttgaaatta gaaagcaacc tcaaactaga ttgttggctg tttctgatgt tcaatatgat
3121 actcctgtta agtctattgc tgctattaat caaaatgaac ctagagctac tgttgctact
3181 tttggagctc ctgaatattt ggctactaga gatggaggag gattgcctag acctattcaa
3241 attgaaagag ttgctggaga aactgatatt gaaactttga ctagacaagt ttatttgttg
3301 tctcaatctc atattcaagt tcataattct actgctagat tgcctattac tactgcttat
3361 gctgatcaag cttctactca tgctactaag ggatatttgg ttcaaactgg agcttttgaa
3421 tctaatgttg gatttttgaa aaggccggcg gccacgaaaa aggccggcca ggcaaaaaag
3481 aaaaagtaac acgtgtgaat tacaggtgac cagctcgaat ttccccgatc gttcaaacat
3541 ttggcaataa agtttcttaa gattgaatcc tgttgccggt cttgcgatga ttatcatata
3601 atttctgttg aattacgtta agcatgtaat aattaacatg taatgcatga cgttatttat
3661 gagatgggtt tttatgatta gagtcccgca attatacatt taatacgcga tagaaaacaa
3721 aatatagcgc gcaaactagg ataaattatc gcgcgcggtg tcatctatgt tactagatcg
3781 ggaattaaac tatcagtgtt tgacaggata tattggcggg taaacctaag agaaaagagc
3841 gtttattaga ataacggata tttaaaaggg cgtgaaaagg tttatccgtt cgtccatttg
3901 tatgtgcatg ccaaccacag ggttcccctc gggatcaaag tactttgatc caacccctcc
3961 gctgctatag tgcagtcggc ttctgacgtt cagtgcagcc gtcttctgaa aacgacatgt
4021 cgcacaagtc ctaagttacg cgacaggctg ccgccctgcc cttttcctgg cgttttcttg
4081 tcgcgtgttt tagtcgcata aagtagaata cttgcgacta gaaccggaga cattacgcca
4141 tgaacaagag cgccgccgct ggcctgctgg gctatgcccg cgtcagcacc gacgaccagg
4201 acttgaccaa ccaacgggcc gaactgcacg cggccggctg caccaagctg ttttccgaga
4261 agatcaccgg caccaggcgc gaccgcccgg agctggccag gatgcttgac cacctacgcc
4321 ctggcgacgt tgtgacagtg accaggctag accgcctggc ccgcagcacc cgcgacctac
4381 tggacattgc cgagcgcatc caggaggccg gcgcgggcct gcgtagcctg gcagagccgt
4441 gggccgacac caccacgccg gccggccgca tggtgttgac cgtgttcgcc ggcattgccg
4501 agttcgagcg ttccctaatc atcgaccgca cccggagcgg gcgcgaggcc gccaaggccc
4561 gaggcgtgaa gtttggcccc cgccctaccc tcaccccggc acagatcgcg cacgcccgcg
4621 agctgatcga ccaggaaggc cgcaccgtga aagaggcggc tgcactgctt ggcgtgcatc
4681 gctcgaccct gtaccgcgca cttgagcgca gcgaggaagt gacgcccacc gaggccaggc
4741 ggcgcggtgc cttccgtgag gacgcattga ccgaggccga cgccctggcg gccgccgaga
4801 atgaacgcca agaggaacaa gcatgaaacc gcaccaggac ggccaggacg aaccgttttt
4861 cattaccgaa gagatcgagg cggagatgat cgcggccggg tacgtgttcg agccgcccgc
4921 gcacgtctca accgtgcggc tgcatgaaat cctggccggt ttgtctgatg ccaagctggc
4981 ggcctggccg gccagcttgg ccgctgaaga aaccgagcgc cgccgtctaa aaaggtgatg
5041 tgtatttgag taaaacagct tgcgtcatgc ggtcgctgcg tatatgatgc gatgagtaaa
5101 taaacaaata cgcaagggga acgcatgaag gttatcgctg tacttaacca gaaaggcggg
5161 tcaggcaaga cgaccatcgc aacccatcta gcccgcgccc tgcaactcgc cggggccgat
5221 gttctgttag tcgattccga tccccagggc agtgcccgcg attgggcggc cgtgcgggaa
5281 gatcaaccgc taaccgttgt cggcatcgac cgcccgacga ttgaccgcga cgtgaaggcc
5341 atcggccggc gcgacttcgt agtgatcgac ggagcgcccc aggcggcgga cttggctgtg
5401 tccgcgatca aggcagccga cttcgtgctg attccggtgc agccaagccc ttacgacata
5461 tgggccaccg ccgacctggt ggagctggtt aagcagcgca ttgaggtcac ggatggaagg
5521 ctacaagcgg cctttgtcgt gtcgcgggcg atcaaaggca cgcgcatcgg cggtgaggtt
5581 gccgaggcgc tggccgggta cgagctgccc attcttgagt cccgtatcac gcagcgcgtg
5641 agctacccag gcactgccgc cgccggcaca accgttcttg aatcagaacc cgagggcgac
5701 gctgcccgcg aggtccaggc gctggccgct gaaattaaat caaaactcat ttgagttaat
5761 gaggtaaaga gaaaatgagc aaaagcacaa acacgctaag tgccggccgt ccgagcgcac
5821 gcagcagcaa ggctgcaacg ttggccagcc tggcagacac gccagccatg aagcgggtca
5881 actttcagtt gccggcggag gatcacacca agctgaagat gtacgcggta cgccaaggca
5941 agaccattac cgagctgcta tctgaataca tcgcgcagct accagagtaa atgagcaaat
6001 gaataaatga gtagatgaat tttagcggct aaaggaggcg gcatggaaaa tcaagaacaa
6061 ccaggcaccg acgccgtgga atgccccatg tgtggaggaa cgggcggttg gccaggcgta
6121 agcggctggg ttgtctgccg gccctgcaat ggcactggaa cccccaagcc cgaggaatcg
6181 gcgtgacggt cgcaaaccat ccggcccggt acaaatcggc gcggcgctgg gtgatgacct
6241 ggtggagaag ttgaaggccg cgcaggccgc ccagcggcaa cgcatcgagg cagaagcacg
6301 ccccggtgaa tcgtggcaag cggccgctga tcgaatccgc aaagaatccc ggcaaccgcc
6361 ggcagccggt gcgccgtcga ttaggaagcc gcccaagggc gacgagcaac cagatttttt
6421 cgttccgatg ctctatgacg tgggcacccg cgatagtcgc agcatcatgg acgtggccgt
6481 tttccgtctg tcgaagcgtg accgacgagc tggcgaggtg atccgctacg agcttccaga
6541 cgggcacgta gaggtttccg cagggccggc cggcatggcc agtgtgtggg attacgacct
6601 ggtactgatg gcggtttccc atctaaccga atccatgaac cgataccggg aagggaaggg
6661 agacaagccc ggccgcgtgt tccgtccaca cgttgcggac gtactcaagt tctgccggcg
6721 agccgatggc ggaaagcaga aagacgacct ggtagaaacc tgcattcggt taaacaccac
6781 gcacgttgcc atgcagcgta cgaagaaggc caagaacggc cgcctggtga cggtatccga
6841 gggtgaagcc ttgattagcc gctacaagat cgtaaagagc gaaaccgggc ggccggagta
6901 catcgagatc gagctagctg attggatgta ccgcgagatc acagaaggca agaacccgga
6961 cgtgctgacg gttcaccccg attacttttt gatcgatccc ggcatcggcc gttttctcta
7021 ccgcctggca cgccgcgccg caggcaaggc agaagccaga tggttgttca agacgatcta
7081 cgaacgcagt ggcagcgccg gagagttcaa gaagttctgt ttcaccgtgc gcaagctgat
7141 cgggtcaaat gacctgccgg agtacgattt gaaggaggag gcggggcagg ctggcccgat
7201 cctagtcatg cgctaccgca acctgatcga gggcgaagca tccgccggtt cctaatgtac
7261 ggagcagatg ctagggcaaa ttgccctagc aggggaaaaa ggtcgaaaag gtctctttcc
7321 tgtggatagc acgtacattg ggaacccaaa gccgtacatt gggaaccgga acccgtacat
7381 tgggaaccca aagccgtaca ttgggaaccg gtcacacatg taagtgactg atataaaaga
7441 gaaaaaaggc gatttttccg cctaaaactc tttaaaactt attaaaactc ttaaaacccg
7501 cctggcctgt gcataactgt ctggccagcg cacagccgaa gagctgcaaa aagcgcctac
7561 ccttcggtcg ctgcgctccc tacgccccgc cgcttcgcgt cggcctatcg cggccgctgg
7621 ccgctcaaaa atggctggcc tacggccagg caatctacca gggcgcggac aagccgcgcc
7681 gtcgccactc gaccgccggc gcccacatca aggcaccctg cctcgcgcgt ttcggtgatg
7741 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg
7801 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg
7861 cagccatgac ccagtcacgt agcgatagcg gagtgtatac tggcttaact atgcggcatc
7921 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag
7981 gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
8041 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
8101 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
8161 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
8221 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
8281 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
8341 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
8401 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
8461 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
8521 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
8581 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
8641 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
8701 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
8761 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
8821 aaactcacgt taagggattt tggtcatgca ttctaggtac taaaacaatt catccagtaa
8881 aatataatat tttattttct cccaatcagg cttgatcccc agtaagtcaa aaaatagctc
8941 gacatactgt tcttccccga tatcctccct gatcgaccgg acgcagaagg caatgtcata
9001 ccacttgtcc gccctgccgc ttctcccaag atcaataaag ccacttactt tgccatcttt
9061 cacaaagatg ttgctgtctc ccaggtcgcc gtgggaaaag acaagttcct cttcgggctt
9121 ttccgtcttt aaaaaatcat acagctcgcg cggatcttta aatggagtgt cttcttccca
9181 gttttcgcaa tccacatcgg ccagatcgtt attcagtaag taatccaatt cggctaagcg
9241 gctgtctaag ctattcgtat agggacaatc cgatatgtcg atggagtgaa agagcctgat
9301 gcactccgca tacagctcga taatcttttc agggctttgt tcatcttcat actcttccga
9361 gcaaaggacg ccatcggcct cactcatgag cagattgctc cagccatcat gccgttcaaa
9421 gtgcaggacc tttggaacag gcagctttcc ttccagccat agcatcatgt ccttttcccg
9481 ttccacatca taggtggtcc ctttataccg gctgtccgtc atttttaaat ataggttttc
9541 attttctccc accagcttat ataccttagc aggagacatt ccttccgtat cttttacgca
9601 gcggtatttt tcgatcagtt ttttcaattc cggtgatatt ctcattttag ccatttatta
9661 tttccttcct cttttctaca gtatttaaag ataccccaag aagctaatta taacaagacg
9721 aactccaatt cactgttcct tgcattctaa aaccttaaat accagaaaac agctttttca
9781 aagttgtttt caaagttggc gtataacata gtatcgacgg agccgatttt gaaaccgcgg
9841 tgatcacagg cagcaacgct ctgtcatcgt tacaatcaac atgctaccct ccgcgagatc
9901 atccgtgttt caaacccggc agcttagttg ccgttcttcc gaatagcatc ggtaacatga
9961 gcaaagtctg ccgccttaca acggctctcc cgctgacgcc gtcccggact gatgggctgc
10021 ctgtatcgag tggtgatttt gtgccgagct gccggtcggg gagctgttgg ctggctggtg
10081 gcaggatata ttgtggtgta aacaaattga cgcttagaca acttaataac acattgcgga
10141 cgtttttaat gtactgaatt aacgccgaat taattcgggg gatctggatt ttagtactgg
10201 attttggttt taggaattag aaattttatt gatagaagta ttttacaaat acaaatacat
10261 actaagggtt tcttatatgc tcaacacatg agcgaaaccc tataggaacc ctaattccct
10321 tatctgggaa ctactcacac attattatgg agaaactcga gcttgtcgat cgacagatcc
10381 ggtcggcatc tactctattt ctttgccctc ggacgagtgc tggggcgtcg gtttccacta
10441 tcggcgagta cttctacaca gccatcggtc cagacggccg cgcttctgcg ggcgatttgt
10501 gtacgcccga cagtcccggc tccggatcgg acgattgcgt cgcatcgacc ctgcgcccaa
10561 gctgcatcat cgaaattgcc gtcaaccaag ctctgataga gttggtcaag accaatgcgg
10621 agcatatacg cccggagtcg tggcgatcct gcaagctccg gatgcctccg ctcgaagtag
10681 cgcgtctgct gctccataca agccaaccac ggcctccaga agaagatgtt ggcgacctcg
10741 tattgggaat ccccgaacat cgcctcgctc cagtcaatga ccgctgttat gcggccattg
10801 tccgtcagga cattgttgga gccgaaatcc gcgtgcacga ggtgccggac ttcggggcag
10861 tcctcggccc aaagcatcag ctcatcgaga gcctgcgcga cggacgcact gacggtgtcg
10921 tccatcacag tttgccagtg atacacatgg ggatcagcaa tcgcgcatat gaaatcacgc
10981 catgtagtgt attgaccgat tccttgcggt ccgaatgggc cgaacccgct cgtctggcta
11041 agatcggccg cagcgatcgc atccatagcc tccgcgaccg gttgtagaac agcgggcagt
11101 tcggtttcag gcaggtcttg caacgtgaca ccctgtgcac ggcgggagat gcaataggtc
11161 aggctctcgc taaactcccc aatgtcaagc acttccggaa tcgggagcgc ggccgatgca
11221 aagtgccgat aaacataacg atctttgtag aaaccatcgg cgcagctatt tacccgcagg
11281 acatatccac gccctcctac atcgaagctg aaagcacgag attcttcgcc ctccgagagc
11341 tgcatcaggt cggagacgct gtcgaacttt tcgatcagaa acttctcgac agacgtcgcg
11401 gtgagttcag gctttttcat atctcattgc cccccgggat ctgcgaaagc tcgagagaga
11461 tagatttgta gagagagact ggtgatttca gcgtgtcctc tccaaatgaa atgaacttcc
11521 ttatatagag gaaggtcttg cgaaggatag tgggattgtg cgtcatccct tacgtcagtg
11581 gagatatcac atcaatccac ttgctttgaa gacgtggttg gaacgtcttc tttttccacg
11641 atgctcctcg tgggtggggg tccatctttg ggaccactgt cggcagaggc atcttgaacg
11701 atagcctttc ctttatcgca atgatggcat ttgtaggtgc caccttcctt ttctactgtc
11761 cttttgatga agtgacagat agctgggcaa tggaatccga ggaggtttcc cgatattacc
11821 ctttgttgaa aagtctcaat agccctttgg tcttctgaga ctgtatcttt gatattcttg
11881 gagtagacga gagtgtcgtg ctccaccatg ttatcacatc aatccacttg ctttgaagac
11941 gtggttggaa cgtcttcttt ttccacgatg ctcctcgtgg gtgggggtcc atctttggga
12001 ccactgtcgg cagaggcatc ttgaacgata gcctttcctt tatcgcaatg atggcatttg
12061 taggtgccac cttccttttc tactgtcctt ttgatgaagt gacagatagc tgggcaatgg
12121 aatccgagga ggtttcccga tattaccctt tgttgaaaag tctcaatagc cctttggtct
12181 tctgagactg tatctttgat attcttggag tagacgagag tgtcgtgctc caccatgttg
12241 gcaagctgct ctagccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
12301 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
12361 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
12421 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
12481 aattcgagct cggtacccgg ggatcctcta gagtcgacct gcaggcatgc aagcttggca
12541 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
12601 cttgcagcac atcccccttt cgccagctgg cgta
//