LOCUS Exported 16084 bp ds-DNA circular SYN 25-AUG-2017
DEFINITION synthetic circular DNA
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 16084)
TITLE Direct Submission
JOURNAL Exported Aug 25, 2017 from MiaoLingPlasmid
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..16084
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_feature 1..25
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
CDS 271..1197
/codon_start=1
/label=mCherry
/translation="MMSGIVLFVTCHSFPTHNPDNNVRASLSLHTHSCMHAFLHVIAMQ
ISFLTYKYKPTLHYTLHSNQNKKTYTNSKTVPTMDNMAIIKEFMRFKVHMEGSVNGHEF
EIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLS
FPEGFKWERVMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEA
SSERMYPEDGALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSH
NEDYTIVEQYERAEGRHSTGGMDELYK"
terminator 1203..1455
/label=MAS terminator
/note="mannopine synthase terminator"
promoter 2568..2783
/label=NOS promoter
/note="nopaline synthase promoter"
CDS 2835..3860
/codon_start=1
/label=Hyg
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDIGGRG
YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPGSW
R"
terminator 3898..4150
/label=NOS terminator
/note="nopaline synthase terminator and poly(A) signal"
promoter 4283..4628
/label=CaMV 35S promoter
/note="strong constitutive promoter from cauliflower mosaic
virus"
CDS 4633..8736
/codon_start=1
/product="Cas9 (Csn1) endonuclease from the Streptococcus
pyogenes Type II CRISPR/Cas system"
/label=Cas9
/note="generates RNA-guided double strand breaks in DNA"
/translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
DATLIHQSITGLYETRIDLSQLGGD"
CDS 8749..8769
/codon_start=1
/product="nuclear localization signal of SV40 large T
antigen"
/label=SV40 NLS
/translation="PKKKRKV"
misc_feature 8780..9249
/label=3A terminator
misc_feature 9519..9543
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA"
CDS 10071..10862
/codon_start=1
/gene="aadA"
/product="aminoglycoside adenylyltransferase (Murphy,
1985)"
/label=Spe
/note="confers resistance to spectinomycin and
streptomycin"
/translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
rep_origin 11106..11694
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
misc_feature 11880..12020
/label=bom
/note="basis of mobility region from pBR322"
rep_origin 12364..12558
/label=pVS1 oriV
/note="origin of replication for the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
CDS complement(12624..13691)
/codon_start=1
/product="replication protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/label=pVS1 RepA
/translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
MRYRNLIEGEASAGS"
CDS complement(14125..14754)
/codon_start=1
/product="stability protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/label=pVS1 StaA
/translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
1 gtttacccgc caatatatcc tgtcaaacac tgatagttta aactagtgaa gtgaagcttg
61 gtctcaacta tgagacctaa gctggcacaa ctatatttcc aacatcacta gctaccatca
121 aaagattgac ttctcatctt actcgattga aaccaaatta acatagggtt tttatttaaa
181 taaaagttta accttctttt taaaaaattg ttcatagtgt catgtcagaa caagagctac
241 aaatcacaca tagcatgcat aagcggagct atgatgagtg gtattgtttt gttcgtcact
301 tgtcactctt ttccaacaca taatcccgac aacaacgtaa gagcatctct ctctctccac
361 acacactcat gcatgcatgc attcttacac gtgattgcca tgcaaatctc ctttctcacc
421 tataaataca aaccaaccct tcactacact cttcactcaa accaaaacaa gaaaacatac
481 acaaatagca aaacggtacc aacaatggat aacatggcca tcatcaagga gttcatgcgc
541 ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc
601 gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc
661 ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacgtg
721 aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg
781 gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg
841 caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc
901 cccgtaatgc agaagaagac catgggctgg gaggcctcct ccgagcggat gtaccccgag
961 gacggcgccc tgaagggcga gatcaagcag aggctgaagc tgaaggacgg cggccactac
1021 gacgctgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac
1081 aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat cgtggaacag
1141 tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta caagtgagag
1201 ctcttggact cccatgttgg caaaggcaac caaacaaaca atgaatgatc cgctcctgca
1261 tatggggcgg tttgagtatt tcaactgcca tttgggctga attgaagaca tgctcctgtc
1321 agaaattccg tgatcttact caatattcag taatctcggc caatatccta aatgtgcgtg
1381 gctttatctg tctttgtatt gtttcatcaa ttcatgtaac gtttgctttt cttatgaatt
1441 ttcaaataaa ttatcgtatt gagaccgaat tctcgtcgac gagtcagtaa taaacggcgt
1501 caaagtggtt gcagccggca cacacgagtc gtgtttatca actcaaagca caaatacttt
1561 tcctcaacct aaaaataagg caattagcca aaaacaactt tgcgtgtaaa caacgctcaa
1621 tacacgtgtc attttattat tagctattgc ttcaccgcct tagctttctc gtgacctagt
1681 cgtcctcgtc ttttcttctt cttcttctat aaaacaatac ccaaagagct cttcttcttc
1741 acaattcaga tttcaatttc tcaaaatctt aaaaactttc tctcaattct ctctaccgtg
1801 atcaaggtaa atttctgtgt tccttattct ctcaaaatct tcgattttgt tttcgttcga
1861 tcccaatttc gtatatgttc tttggtttag attctgttaa tcttagatcg aagacgattt
1921 tctgggtttg atcgttagat atcatcttaa ttctcgatta gggtttcata gatatcatcc
1981 gatttgttca aataatttga gttttgtcga ataattactc ttcgatttgt gatttctatc
2041 tagatctggt gttagtttct agtttgtgcg atcgaatttg tcgattaatc tgagtttttc
2101 tgattaacag ctcgcaattg acctaactga gtaagctagc ttgttcgagt attatggcat
2161 tgggaaaact gtttttcttg taccatttgt tgtgcttgta atttactgtg ttttttattc
2221 ggttttcgct atcgaactgt gaaatggaaa tggatggaga agagttaatg aatgatatgg
2281 tccttttgtt cattctcaaa ttaatattat ttgttttttc tcttatttgt tgtgtgttga
2341 atttgaaatt ataagagata tgcaaacatt ttgttttgag taaaaatgtg tcaaatcgtg
2401 gcctctaatg accgaagtta atatgaggag taaaacacta gatccccaaa caagcttgaa
2461 actgaaggcg ggaaacgaca atctgatcat gagcggagaa ttaagggagt cacgttatga
2521 cccccgccga tgacgcggga caagccgttt tacgtttgga actgacagaa ccgcaacgtt
2581 gaaggagcca ctcagccgcg ggtttctgga gtttaatgag ctaagcacat acgtcagaaa
2641 ccattattgc gcgttcaaaa gtcgcctaag gtcactatca gctagcaaat atttcttgtc
2701 aaaaatgctc cactgacgtt ccataaattc ccctcggtat ccaattagag tctcatattc
2761 actctcaatc caaataatct gcaccggatc cgctagagga tctcgacctg caagatcccg
2821 gggggcaatg agatatgaaa aagcctgaac tcaccgcgac gtctgtcgag aagtttctga
2881 tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc ggagggcgaa gaatctcgtg
2941 ctttcagctt cgatatagga gggcgtggat atgtcctgcg ggtaaatagc tgcgccgatg
3001 gtttctacaa agatcgttat gtttatcggc actttgcatc ggccgcgctc ccgattccgg
3061 aagtgcttga cattggggaa ttcagcgaga gcctgaccta ttgcatctcc cgccgtgcac
3121 agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc cgctgttctg cagccggtcg
3181 cggaggccat ggatgcgatc gctgcggccg atcttagcca gacgagcggg ttcggcccat
3241 tcggaccgca aggaatcggt caatacacta catggcgtga tttcatatgc gcgattgctg
3301 atccccatgt gtatcactgg caaactgtga tggacgacac cgtcagtgcg tccgtcgcgc
3361 aggctctcga tgagctgatg ctttgggccg aggactgccc cgaagtccgg cacctcgtgc
3421 acgcggattt cggctccaac aatgtcctga cggacaatgg ccgcataaca gcggtcattg
3481 actggagcga ggcgatgttc ggggattccc aatacgaggt cgccaacatc ttcttctgga
3541 ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt cgagcggagg catccggagc
3601 ttgcaggatc gccgcggctc cgggcgtata tgctccgcat tggtcttgac caactctatc
3661 agagcttggt tgacggcaat ttcgatgatg cagcttgggc gcagggtcga tgcgacgcaa
3721 tcgtccgatc cggagccggg actgtcgggc gtacacaaat cgcccgcaga agcgcggccg
3781 tctggaccga tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc cccagcactc
3841 gtccgggatc ttggaggtga tgtaacatga tcacaagctg atcccccgaa tttccccgat
3901 cgttcaaaca tttggcaata aagtttctta agattgaatc ctgttgccgg tcttgcgatg
3961 attatcatat aatttctgtt gaattacgtt aagcatgtaa taattaacat gtaatgcatg
4021 acgttattta tgagatgggt ttttatgatt agagtcccgc aattatacat ttaatacgcg
4081 atagaaaaca aaatatagcg cgcaaactag gataaattat cgcgcgcggt gtcatctatg
4141 ttactagatc gggaattgat cccccctcga cagcttgcat gccagcttgg gctgcaggtc
4201 gacgcgtcaa catggtggag cacgacactc tcgtctactc caagaatatc aaagatacag
4261 tctcagaaga ccaaagggct attgagactt ttcaacaaag ggtaatatcg ggaaacctcc
4321 tcggattcca ttgcccagct atctgtcact tcatcaaaag gacagtagaa aaggaaggtg
4381 gcacctacaa atgccatcat tgcgataaag gaaaggctat cgttcaagat gcctctgccg
4441 acagtggtcc caaagatgga cccccaccca cgaggagcat cgtggaaaaa gaagacgttc
4501 caaccacgtc ttcaaagcaa gtggattgat gtgatatctc cactgacgta agggatgacg
4561 cacaatccca ctatccttcg caagaccctt cctctatata aggaagttca tttcatttgg
4621 agaggacacg atatggacaa gaagtactcc attgggctcg atatcggcac aaacagcgtc
4681 ggctgggccg tcattacgga cgagtacaag gtgccgagca aaaaattcaa agttctgggc
4741 aataccgatc gccacagcat aaagaagaac ctcattggcg ccctcctgtt cgactccggg
4801 gagacggccg aagccacgcg gctcaaaaga acagcacggc gcagatatac ccgcagaaag
4861 aatcggatct gctacctgca ggagatcttt agtaatgaga tggctaaggt ggatgactct
4921 ttcttccata ggctggagga gtcctttttg gtggaggagg ataaaaagca cgagcgccac
4981 ccaatctttg gcaatatcgt ggacgaggtg gcgtaccatg aaaagtaccc aaccatatat
5041 catctgagga agaagcttgt agacagtact gataaggctg acttgcggtt gatctatctc
5101 gcgctggcgc atatgatcaa atttcgggga cacttcctca tcgaggggga cctgaaccca
5161 gacaacagcg atgtcgacaa actctttatc caactggttc agacttacaa tcagcttttc
5221 gaagagaacc cgatcaacgc atccggagtt gacgccaaag caatcctgag cgctaggctg
5281 tccaaatccc ggcggctcga aaacctcatc gcacagctcc ctggggagaa gaagaacggc
5341 ctgtttggta atcttatcgc cctgtcactc gggctgaccc ccaactttaa atctaacttc
5401 gacctggccg aagatgccaa gcttcaactg agcaaagaca cctacgatga tgatctcgac
5461 aatctgctgg cccagatcgg cgaccagtac gcagaccttt ttttggcggc aaagaacctg
5521 tcagacgcca ttctgctgag tgatattctg cgagtgaaca cggagatcac caaagctccg
5581 ctgagcgcta gtatgatcaa gcgctatgat gagcaccacc aagacttgac tttgctgaag
5641 gcccttgtca gacagcaact gcctgagaag tacaaggaaa ttttcttcga tcagtctaaa
5701 aatggctacg ccggatacat tgacggcgga gcaagccagg aggaatttta caaatttatt
5761 aagcccatct tggaaaaaat ggacggcacc gaggagctgc tggtaaagct taacagagaa
5821 gatctgttgc gcaaacagcg cactttcgac aatggaagca tcccccacca gattcacctg
5881 ggcgaactgc acgctatcct caggcggcaa gaggatttct accccttttt gaaagataac
5941 agggaaaaga ttgagaaaat cctcacattt cggataccct actatgtagg ccccctcgcc
6001 cggggaaatt ccagattcgc gtggatgact cgcaaatcag aagagaccat cactccctgg
6061 aacttcgagg aagtcgtgga taagggggcc tctgcccagt ccttcatcga aaggatgact
6121 aactttgata aaaatctgcc taacgaaaag gtgcttccta aacactctct gctgtacgag
6181 tacttcacag tttataacga gctcaccaag gtcaaatacg tcacagaagg gatgagaaag
6241 ccagcattcc tgtctggaga gcagaagaaa gctatcgtgg acctcctctt caagacgaac
6301 cggaaagtta ccgtgaaaca gctcaaagaa gactatttca aaaagattga atgtttcgac
6361 tctgttgaaa tcagcggagt ggaggatcgc ttcaacgcat ccctgggaac gtatcacgat
6421 ctcctgaaaa tcattaaaga caaggacttc ctggacaatg aggagaacga ggacattctt
6481 gaggacattg tcctcaccct tacgttgttt gaagataggg agatgattga agaacgcttg
6541 aaaacttacg ctcatctctt cgacgacaaa gtcatgaaac agctcaagag gcgccgatat
6601 acaggatggg ggcggctgtc aagaaaactg atcaatggga tccgagacaa gcagagtgga
6661 aagacaatcc tggattttct taagtccgat ggatttgcca accggaactt catgcagttg
6721 atccatgatg actctctcac ctttaaggag gacatccaga aagcacaagt ttctggccag
6781 ggggacagtc ttcacgagca catcgctaat cttgcaggta gcccagctat caaaaaggga
6841 atactgcaga ccgttaaggt cgtggatgaa ctcgtcaaag taatgggaag gcataagccc
6901 gagaatatcg ttatcgagat ggcccgagag aaccaaacta cccagaaggg acagaagaac
6961 agtagggaaa ggatgaagag gattgaagag ggtataaaag aactggggtc ccaaatcctt
7021 aaggaacacc cagttgaaaa cacccagctt cagaatgaga agctctacct gtactacctg
7081 cagaacggca gggacatgta cgtggatcag gaactggaca tcaatcggct ctccgactac
7141 gacgtggatc atatcgtgcc ccagtctttt ctcaaagatg attctattga taataaagtg
7201 ttgacaagat ccgataaaaa tagagggaag agtgataacg tcccctcaga agaagttgtc
7261 aagaaaatga aaaattattg gcggcagctg ctgaacgcca aactgatcac acaacggaag
7321 ttcgataatc tgactaaggc tgaacgaggt ggcctgtctg agttggataa agccggcttc
7381 atcaaaaggc agcttgttga gacacgccag atcaccaagc acgtggccca aattctcgat
7441 tcacgcatga acaccaagta cgatgaaaat gacaaactga ttcgagaggt gaaagttatt
7501 actctgaagt ctaagctggt ctcagatttc agaaaggact ttcagtttta taaggtgaga
7561 gagatcaaca attaccacca tgcgcatgat gcctacctga atgcagtggt aggcactgca
7621 cttatcaaaa aatatcccaa gcttgaatct gaatttgttt acggagacta taaagtgtac
7681 gatgttagga aaatgatcgc aaagtctgag caggaaatag gcaaggccac cgctaagtac
7741 ttcttttaca gcaatattat gaattttttc aagaccgaga ttacactggc caatggagag
7801 attcggaagc gaccacttat cgaaacaaac ggagaaacag gagaaatcgt gtgggacaag
7861 ggtagggatt tcgcgacagt ccggaaggtc ctgtccatgc cgcaggtgaa catcgttaaa
7921 aagaccgaag tacagaccgg aggcttctcc aaggaaagta tcctcccgaa aaggaacagc
7981 gacaagctga tcgcacgcaa aaaagattgg gaccccaaga aatacggcgg attcgattct
8041 cctacagtcg cttacagtgt actggttgtg gccaaagtgg agaaagggaa gtctaaaaaa
8101 ctcaaaagcg tcaaggaact gctgggcatc acaatcatgg agcgatcaag cttcgaaaaa
8161 aaccccatcg actttctcga ggcgaaagga tataaagagg tcaaaaaaga cctcatcatt
8221 aagcttccca agtactctct ctttgagctt gaaaacggcc ggaaacgaat gctcgctagt
8281 gcgggcgagc tgcagaaagg taacgagctg gcactgccct ctaaatacgt taatttcttg
8341 tatctggcca gccactatga aaagctcaaa gggtctcccg aagataatga gcagaagcag
8401 ctgttcgtgg aacaacacaa acactacctt gatgagatca tcgagcaaat aagcgaattc
8461 tccaaaagag tgatcctcgc cgacgctaac ctcgataagg tgctttctgc ttacaataag
8521 cacagggata agcccatcag ggagcaggca gaaaacatta tccacttgtt tactctgacc
8581 aacttgggcg cgcctgcagc cttcaagtac ttcgacacca ccatagacag aaagcggtac
8641 acctctacaa aggaggtcct ggacgccaca ctgattcatc agtcaattac ggggctctat
8701 gaaacaagaa tcgacctctc tcagctcggt ggagacagca gggctgaccc caagaagaag
8761 aggaaggtgt gatcgatcca ggcctcccag ctttcgtccg tatcatcggt ttcgacaacg
8821 ttcgtcaagt tcaatgcatc agtttcattg cccacacacc agaatcctac taagtttgag
8881 tattatggca ttggaaaagc tgttttcttc tatcatttgt tctgcttgta atttactgtg
8941 ttctttcagt ttttgttttc ggacatcaaa atgcaaatgg atggataaga gttaataaat
9001 gatatggtcc ttttgttcat tctcaaatta ttattatctg ttgtttttac tttaatgggt
9061 tgaatttaag taagaaagga actaacagtg tgatattaag gtgcaatgtt agacatataa
9121 aacagtcttt cacctctctt tggttatgtc ttgaattggt ttgtttcttc acttatctgt
9181 gtaatcaagt ttactatgag tctatgatca agtaattatg caatcaagtt aagtacagta
9241 taggcttttt gtgtcgaggg ggtaccgagt cgaggaattc actggccgtc gttttacaac
9301 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
9361 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
9421 gcctgaatgg cgggtaccga gctcgaattc aattcggcgt taattcagta cattaaaaac
9481 gtccgcaatg tgttattaag ttgtctaagc gtcaatttgt ttacaccaca atatatcctg
9541 ccaccagcca gccaacagct ccccgaccgg cagctcggca caaaatcacc actcgataca
9601 ggcagcccat cagtccggga cggcgtcagc gggagagccg ttgtaaggcg gcagactttg
9661 ctcatgttac cgatgctatt cggaagaacg gcaactaagc tgccgggttt gaaacacgga
9721 tgatctcgcg gagggtagca tgttgattgt aacgatgaca gagcgttgct gcctgtgatc
9781 aattcgggca cgaacccagt ggacataagc ctcgttcggt tcgtaagctg taatgcaagt
9841 agcgtaactg ccgtcacgca actggtccag aaccttgacc gaacgcagcg gtggtaacgg
9901 cgcagtggcg gttttcatgg cttcttgtta tgacatgttt ttttggggta cagtctatgc
9961 ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca
10021 gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa gttaaacatc atgggggaag
10081 cggtgatcgc cgaagtatcg actcaactat cagaggtagt tggcgtcatc gagcgccatc
10141 tcgaaccgac gttgctggcc gtacatttgt acggctccgc agtggatggc ggcctgaagc
10201 cacacagtga tattgatttg ctggttacgg tgaccgtaag gcttgatgaa acaacgcggc
10261 gagctttgat caacgacctt ttggaaactt cggcttcccc tggagagagc gagattctcc
10321 gcgctgtaga agtcaccatt gttgtgcacg acgacatcat tccgtggcgt tatccagcta
10381 agcgcgaact gcaatttgga gaatggcagc gcaatgacat tcttgcaggt atcttcgagc
10441 cagccacgat cgacattgat ctggctatct tgctgacaaa agcaagagaa catagcgttg
10501 ccttggtagg tccagcggcg gaggaactct ttgatccggt tcctgaacag gatctatttg
10561 aggcgctaaa tgaaacctta acgctatgga actcgccgcc cgactgggct ggcgatgagc
10621 gaaatgtagt gcttacgttg tcccgcattt ggtacagcgc agtaaccggc aaaatcgcgc
10681 cgaaggatgt cgctgccgac tgggcaatgg agcgcctgcc ggcccagtat cagcccgtca
10741 tacttgaagc tagacaggct tatcttggac aagaagaaga tcgcttggcc tcgcgcgcag
10801 atcagttgga agaatttgtc cactacgtga aaggcgagat caccaaggta gtcggcaaat
10861 aatgtctagc tagaaattcg ttcaagccga cgccgcttcg ccggcgttaa ctcaagcgat
10921 tagatgcact aagcacataa ttgctcacag ccaaactatc aggtcaagtc tgcttttatt
10981 atttttaagc gtgcataata agccctacac aaattgggag atatatcatg catgaccaaa
11041 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
11101 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
11161 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
11221 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
11281 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
11341 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
11401 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
11461 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
11521 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
11581 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
11641 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
11701 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
11761 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
11821 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
11881 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact
11941 ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac
12001 gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
12061 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
12121 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gggtgccttg atgtgggcgc
12181 cggcggtcga gtggcgacgg cgcggcttgt ccgcgccctg gtagattgcc tggccgtagg
12241 ccagccattt ttgagcggcc agcggccgcg ataggccgac gcgaagcggc ggggcgtagg
12301 gagcgcagcg accgaagggt aggcgctttt tgcagctctt cggctgtgcg ctggccagac
12361 agttatgcac aggccaggcg ggttttaaga gttttaataa gttttaaaga gttttaggcg
12421 gaaaaatcgc cttttttctc ttttatatca gtcacttaca tgtgtgaccg gttcccaatg
12481 tacggctttg ggttcccaat gtacgggttc cggttcccaa tgtacggctt tgggttccca
12541 atgtacgtgc tatccacagg aaagagacct tttcgacctt tttcccctgc tagggcaatt
12601 tgccctagca tctgctccgt acattaggaa ccggcggatg cttcgccctc gatcaggttg
12661 cggtagcgca tgactaggat cgggccagcc tgccccgcct cctccttcaa atcgtactcc
12721 ggcaggtcat ttgacccgat cagcttgcgc acggtgaaac agaacttctt gaactctccg
12781 gcgctgccac tgcgttcgta gatcgtcttg aacaaccatc tggcttctgc cttgcctgcg
12841 gcgcggcgtg ccaggcggta gagaaaacgg ccgatgccgg gatcgatcaa aaagtaatcg
12901 gggtgaaccg tcagcacgtc cgggttcttg ccttctgtga tctcgcggta catccaatca
12961 gctagctcga tctcgatgta ctccggccgc ccggtttcgc tctttacgat cttgtagcgg
13021 ctaatcaagg cttcaccctc ggataccgtc accaggcggc cgttcttggc cttcttcgta
13081 cgctgcatgg caacgtgcgt ggtgtttaac cgaatgcagg tttctaccag gtcgtctttc
13141 tgctttccgc catcggctcg ccggcagaac ttgagtacgt ccgcaacgtg tggacggaac
13201 acgcggccgg gcttgtctcc cttcccttcc cggtatcggt tcatggattc ggttagatgg
13261 gaaaccgcca tcagtaccag gtcgtaatcc cacacactgg ccatgccggc cggccctgcg
13321 gaaacctcta cgtgcccgtc tggaagctcg tagcggatca cctcgccagc tcgtcggtca
13381 cgcttcgaca gacggaaaac ggccacgtcc atgatgctgc gactatcgcg ggtgcccacg
13441 tcatagagca tcggaacgaa aaaatctggt tgctcgtcgc ccttgggcgg cttcctaatc
13501 gacggcgcac cggctgccgg cggttgccgg gattctttgc ggattcgatc agcggccgct
13561 tgccacgatt caccggggcg tgcttctgcc tcgatgcgtt gccgctgggc ggcctgcgcg
13621 gccttcaact tctccaccag gtcatcaccc agcgccgcgc cgatttgtac cgggccggat
13681 ggtttgcgac cgtcacgccg attcctcggg cttgggggtt ccagtgccat tgcagggccg
13741 gcagacaacc cagccgctta cgcctggcca accgcccgtt cctccacaca tggggcattc
13801 cacggcgtcg gtgcctggtt gttcttgatt ttccatgccg cctcctttag ccgctaaaat
13861 tcatctactc atttattcat ttgctcattt actctggtag ctgcgcgatg tattcagata
13921 gcagctcggt aatggtcttg ccttggcgta ccgcgtacat cttcagcttg gtgtgatcct
13981 ccgccggcaa ctgaaagttg acccgcttca tggctggcgt gtctgccagg ctggccaacg
14041 ttgcagcctt gctgctgcgt gcgctcggac ggccggcact tagcgtgttt gtgcttttgc
14101 tcattttctc tttacctcat taactcaaat gagttttgat ttaatttcag cggccagcgc
14161 ctggacctcg cgggcagcgt cgccctcggg ttctgattca agaacggttg tgccggcggc
14221 ggcagtgcct gggtagctca cgcgctgcgt gatacgggac tcaagaatgg gcagctcgta
14281 cccggccagc gcctcggcaa cctcaccgcc gatgcgcgtg cctttgatcg cccgcgacac
14341 gacaaaggcc gcttgtagcc ttccatccgt gacctcaatg cgctgcttaa ccagctccac
14401 caggtcggcg gtggcccata tgtcgtaagg gcttggctgc accggaatca gcacgaagtc
14461 ggctgccttg atcgcggaca cagccaagtc cgccgcctgg ggcgctccgt cgatcactac
14521 gaagtcgcgc cggccgatgg ccttcacgtc gcggtcaatc gtcgggcggt cgatgccgac
14581 aacggttagc ggttgatctt cccgcacggc cgcccaatcg cgggcactgc cctggggatc
14641 ggaatcgact aacagaacat cggccccggc gagttgcagg gcgcgggcta gatgggttgc
14701 gatggtcgtc ttgcctgacc cgcctttctg gttaagtaca gcgataacct tcatgcgttc
14761 cccttgcgta tttgtttatt tactcatcgc atcatatacg cagcgaccgc atgacgcaag
14821 ctgttttact caaatacaca tcaccttttt agacggcggc gctcggtttc ttcagcggcc
14881 aagctggccg gccaggccgc cagcttggca tcagacaaac cggccaggat ttcatgcagc
14941 cgcacggttg agacgtgcgc gggcggctcg aacacgtacc cggccgcgat catctccgcc
15001 tcgatctctt cggtaatgaa aaacggttcg tcctggccgt cctggtgcgg tttcatgctt
15061 gttcctcttg gcgttcattc tcggcggccg ccagggcgtc ggcctcggtc aatgcgtcct
15121 cacggaaggc accgcgccgc ctggcctcgg tgggcgtcac ttcctcgctg cgctcaagtg
15181 cgcggtacag ggtcgagcga tgcacgccaa gcagtgcagc cgcctctttc acggtgcggc
15241 cttcctggtc gatcagctcg cgggcgtgcg cgatctgtgc cggggtgagg gtagggcggg
15301 ggccaaactt cacgcctcgg gccttggcgg cctcgcgccc gctccgggtg cggtcgatga
15361 ttagggaacg ctcgaactcg gcaatgccgg cgaacacggt caacaccatg cggccggccg
15421 gcgtggtggt gtcggcccac ggctctgcca ggctacgcag gcccgcgccg gcctcctgga
15481 tgcgctcggc aatgtccagt aggtcgcggg tgctgcgggc caggcggtct agcctggtca
15541 ctgtcacaac gtcgccaggg cgtaggtggt caagcatcct ggccagctcc gggcggtcgc
15601 gcctggtgcc ggtgatcttc tcggaaaaca gcttggtgca gccggccgcg tgcagttcgg
15661 cccgttggtt ggtcaagtcc tggtcgtcgg tgctgacgcg ggcatagccc agcaggccag
15721 cggcggcgct cttgttcatg gcgtaatgtc tccggttcta gtcgcaagta ttctacttta
15781 tgcgactaaa acacgcgaca agaaaacgcc aggaaaaggg cagggcggca gcctgtcgcg
15841 taacttagga cttgtgcgac atgtcgtttt cagaagacgg ctgcactgaa cgtcagaagc
15901 cgactgcact atagcagcgg aggggttgga tcaaagtact ttaaagtact ttaaagtact
15961 ttaaagtact ttgatcccga ggggaaccct gtggttggca tgcacataca aatggacgaa
16021 cggataaacc ttttcacgcc cttttaaata tccgattatt ctaataaacg ctcttttctc
16081 ttag
//