LOCUS Exported 11517 bp ds-DNA circular SYN 28-DEC-2017
DEFINITION synthetic circular DNA
FEATURES Location/Qualifiers
source 1..11517
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_feature 1..25
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
promoter 216..262
/label=minimal CaMV 35S promoter
/note="minimal 35S promoter from cauliflower mosaic virus"
CDS 291..542
/codon_start=1
/gene="lexA (truncated)"
/product="DNA-binding domain of E. coli LexA"
/label=LexA BD
/note="binds the 16-bp palindromic sequence
5'-CTGTATATATATACAG-3'"
/translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAA"
CDS 558..791
/codon_start=1
/gene="UL48"
/product="transcriptional activation domain of herpes
simplex virus protein VP16 (Triezenberg et al., 1988;
Cousens et al., 1989)"
/label=VP16 AD
/translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
CDS 849..1742
/codon_start=1
/gene="ESR1"
/product="estrogen nuclear receptor alpha ligand-binding
domain"
/label=ER-LBD
/translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPVKL
LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
GMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
QKYYITGEAEGFPATV"
promoter 2294..2510
/label=NOS promoter
/note="nopaline synthase promoter"
CDS 2525..3550
/codon_start=1
/gene="aph(4)-Ia"
/product="aminoglycoside phosphotransferase from E. coli"
/label=HygR
/note="confers resistance to hygromycin"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
E"
terminator 3552..3804
/label=NOS terminator
/note="nopaline synthase terminator and poly(A) signal"
promoter 4127..4173
/label=minimal CaMV 35S promoter
/note="minimal 35S promoter from cauliflower mosaic virus"
misc_feature 4199..4237
/label=MCS
misc_feature 4983..5007
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA"
CDS 5535..6326
/codon_start=1
/gene="aadA"
/product="aminoglycoside adenylyltransferase (Murphy,
1985)"
/label=Spe
/note="confers resistance to spectinomycin and
streptomycin"
/translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
rep_origin 6570..7158
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
misc_feature 7344..7484
/label=bom
/note="basis of mobility region from pBR322"
rep_origin 7828..8022
/label=pVS1 oriV
/note="origin of replication for the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
CDS complement(8088..9155)
/codon_start=1
/product="replication protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/label=pVS1 RepA
/translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
MRYRNLIEGEASAGS"
CDS complement(9589..10218)
/codon_start=1
/product="stability protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/label=pVS1 StaA
/translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
1 gtttacccgc caatatatcc tgtcaaacac tgatagttta aactgaaggc gggaaacgac
61 aatctgatcc aagctcaagc taagcttgca tgcctgcagg atatcgtgga tccaagcttg
121 ccacgtgccg ccacgtgccg ccacgtgccg ccacgtgcct ctagaggatc catctccact
181 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga
241 agttcatttc atttggagag gacacgctgg gatccccaat tccgggcgga atgaaagcgt
301 taacggccag gcaacaagag gtgtttgatc tcatccgtga tcacatcagc cagacaggta
361 tgccgccgac gcgtgcggaa atcgcgcagc gtttggggtt ccgttcccca aacgcggctg
421 aagaacatct gaaggcgctg gcacgcaaag gcgttattga aattgtttcc ggcgcatcac
481 gcgggattcg tctgttgcag gaagaggaag aagggttgcc gctggtaggt cgtgtggctg
541 ccggtgaacc gtcgagcgcc cccccgaccg atgtcagcct gggggacgag ctccacttag
601 acggcgagga cgtggcgatg gcgcatgccg acgcgctaga cgatttcgat ctggacatgt
661 tgggggacgg ggattccccg ggtccgggat ttacccccca cgactccgcc ccctacggcg
721 ctctggatat ggccgacttc gagtttgagc agatgtttac cgatgccctt ggaattgacg
781 agtacggtgg ggatccgtct gctggagaca tgagagctgc caacctttgg ccaagcccgc
841 tcatgatcaa acgctctaag aagaacagcc tggccttgtc cctgacggcc gaccagatgg
901 tcagtgcctt gttggatgct gagcccccca tactctattc cgagtatgat cctaccagac
961 ccttcagtga agcttcgatg atgggcttac tgaccaacct ggcagacagg gagctggttc
1021 acatgatcaa ctgggcgaag agggtgccag gctttgtgga tttgaccctc catgatcagg
1081 tccaccttct agaatgtgcc tggctagaga tcctgatgat tggtctcgtc tggcgctcca
1141 tggagcaccc agtgaagcta ctgtttgctc ctaacttgct cttggacagg aaccagggaa
1201 aatgtgtaga gggcatggtg gagatcttcg acatgctgct ggctacatca tctcggttcc
1261 gcatgatgaa tctgcaggga gaggagtttg tgtgcctcaa atctattatt ttgcttaatt
1321 ctggagtgta cacatttctg tccagcaccc tgaagtctct ggaagagaag gaccatatcc
1381 accgagtcct ggacaagatc acagacactt tgatccacct gatggccaag gcaggcctga
1441 ccctgcagca gcagcaccag cggctggccc agctcctcct catcctctcc cacatcaggc
1501 acatgagtaa caaaggcatg gagcatctgt acagcatgaa gtgcaagaac gtggtgcccc
1561 tctatgacct gctgctggag atgctggacg cccaccgcct acatgcgccc actagccgtg
1621 gaggggcatc cgtggaggag acggaccaaa gccacttggc cactgcgggc tctacttcat
1681 cgcattcctt gcaaaagtat tacatcacgg gggaggcaga gggtttccct gccacagtct
1741 gagagctccc tggcgaattc ccagagatgt tagctgaaat catcactaat cagataccaa
1801 aatattcaaa tggaaatatc aaaaagcttc tgtttcatca aaaatgactc gacctaactg
1861 agtaagctag cttgttcgag tattatggca ttgggaaaac tgtttttctt gtaccatttg
1921 ttgtgcttgt aatttactgt gttttttatt cggttttcgc tatcgaactg tgaaatggaa
1981 atggatggag aagagttaat gaatgatatg gtccttttgt tcattctcaa attaatatta
2041 tttgtttttt ctcttatttg ttgtgtgttg aatttgaaat tataagagat atgcaaacat
2101 tttgttttga gtaaaaatgt gtcaaatcgt ggcctctaat gaccgaagtt aatatgagga
2161 gtaaaacatc ccaaacaagc ttggaaactg aaggcgggaa acgacaatct gatcatgagc
2221 ggagaattaa gggagtcacg ttatgacccc cgccgatgac gcgggacaag ccgttttacg
2281 tttggaactg acagaaccgc aacgattgaa ggagccactc agccgcgggt ttctggagtt
2341 taatgagcta agcacatacg tcagaaacca ttattgcgcg ttcaaaagtc gcctaaggtc
2401 actatcagct agcaaatatt tcttgtcaaa aatgctccac tgacgttcca taaattcccc
2461 tcggtatcca attagagtct catattcact ctcaatccaa ataatctgca ccggatcccc
2521 tagaatgaaa aagcctgaac tcaccgcgac gtctgtcgag aagtttctga tcgaaaagtt
2581 cgacagcgtc tccgacctga tgcagctctc ggagggcgaa gaatctcgtg ctttcagctt
2641 cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc tgcgccgatg gtttctacaa
2701 agatcgttat gtttatcggc actttgcatc ggccgcgctc ccgattccgg aagtgcttga
2761 cattggggaa ttcagcgaga gcctgaccta ttgcatctcc cgccgtgcac agggtgtcac
2821 gttgcaagac ctgcctgaaa ccgaactgcc cgctgttctg cagccggtcg cggaggccat
2881 ggatgcgatc gctgcggccg atcttagcca gacgagcggg ttcggcccat tcggaccgca
2941 aggaatcggt caatacacta catggcgtga tttcatatgc gcgattgctg atccccatgt
3001 gtatcactgg caaactgtga tggacgacac cgtcagtgcg tccgtcgcgc aggctctcga
3061 tgagctgatg ctttgggccg aggactgccc cgaagtccgg cacctcgtgc acgcggattt
3121 cggctccaac aatgtcctga cggacaatgg ccgcataaca gcggtcattg actggagcga
3181 ggcgatgttc ggggattccc aatacgaggt cgccaacatc ttcttctgga ggccgtggtt
3241 ggcttgtatg gagcagcaga cgcgctactt cgagcggagg catccggagc ttgcaggatc
3301 gccgcggctc cgggcgtata tgctccgcat tggtcttgac caactctatc agagcttggt
3361 tgacggcaat ttcgatgatg cagcttgggc gcagggtcga tgcgacgcaa tcgtccgatc
3421 cggagccggg actgtcgggc gtacacaaat cgcccgcaga agcgcggccg tctggaccga
3481 tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc cccagcactc gtccgagggc
3541 aaaggaatag cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg
3601 ccggtcttgc gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta
3661 acatgtaatg catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat
3721 acatttaata cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg
3781 cggtgtcatc tatgttacta gatcggggaa ttgatccccc ctcgacagct tgcatgccag
3841 cttgggctgc aggtcgaggc taaaaaacta atcgcattat catcccctcg acgtactgta
3901 catataacca ctggttttat atacagcagt actgtacata taaccactgg ttttatatac
3961 agcagtcgac gtactgtaca tataaccact ggttttatat acagcagtac tgtacatata
4021 accactggtt ttatatacag cagtcgaggt aagattagat atggatatgt atatggatat
4081 gtatatggtg gtaatgccat gtaatatgct cgactctagg atcttcgcaa gacccttcct
4141 ctatataagg aagttcattt catttggaga ggacacgctg aagctagtcg actctagcct
4201 cgaggcgcgc cgggcccata cgcgttaatt aactagtcga tccaggcctc ccagctttcg
4261 tccgtatcat cggtttcgac aacgttcgtc aagttcaatg catcagtttc attgcccaca
4321 caccagaatc ctactaagtt tgagtattat ggcattggaa aagctgtttt cttctatcat
4381 ttgttctgct tgtaatttac tgtgttcttt cagtttttgt tttcggacat caaaatgcaa
4441 atggatggat aagagttaat aaatgatatg gtccttttgt tcattctcaa attattatta
4501 tctgttgttt ttactttaat gggttgaatt taagtaagaa aggaactaac agtgtgatat
4561 taaggtgcaa tgttagacat ataaaacagt ctttcacctc tctttggtta tgtcttgaat
4621 tggtttgttt cttcacttat ctgtgtaatc aagtttacta tgagtctatg atcaagtaat
4681 tatgcaatca agttaagtac agtataggct ttttgtgtcg agggggtacc gagtcgagga
4741 attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
4801 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
4861 atcgcccttc ccaacagttg cgcagcctga atggcgggta ccgagctcga attcaattcg
4921 gcgttaattc agtacattaa aaacgtccgc aatgtgttat taagttgtct aagcgtcaat
4981 ttgtttacac cacaatatat cctgccacca gccagccaac agctccccga ccggcagctc
5041 ggcacaaaat caccactcga tacaggcagc ccatcagtcc gggacggcgt cagcgggaga
5101 gccgttgtaa ggcggcagac tttgctcatg ttaccgatgc tattcggaag aacggcaact
5161 aagctgccgg gtttgaaaca cggatgatct cgcggagggt agcatgttga ttgtaacgat
5221 gacagagcgt tgctgcctgt gatcaattcg ggcacgaacc cagtggacat aagcctcgtt
5281 cggttcgtaa gctgtaatgc aagtagcgta actgccgtca cgcaactggt ccagaacctt
5341 gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttctt gttatgacat
5401 gtttttttgg ggtacagtct atgcctcggg catccaagca gcaagcgcgt tacgccgtgg
5461 gtcgatgttt gatgttatgg agcagcaacg atgttacgca gcagggcagt cgccctaaaa
5521 caaagttaaa catcatgggg gaagcggtga tcgccgaagt atcgactcaa ctatcagagg
5581 tagttggcgt catcgagcgc catctcgaac cgacgttgct ggccgtacat ttgtacggct
5641 ccgcagtgga tggcggcctg aagccacaca gtgatattga tttgctggtt acggtgaccg
5701 taaggcttga tgaaacaacg cggcgagctt tgatcaacga ccttttggaa acttcggctt
5761 cccctggaga gagcgagatt ctccgcgctg tagaagtcac cattgttgtg cacgacgaca
5821 tcattccgtg gcgttatcca gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg
5881 acattcttgc aggtatcttc gagccagcca cgatcgacat tgatctggct atcttgctga
5941 caaaagcaag agaacatagc gttgccttgg taggtccagc ggcggaggaa ctctttgatc
6001 cggttcctga acaggatcta tttgaggcgc taaatgaaac cttaacgcta tggaactcgc
6061 cgcccgactg ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc atttggtaca
6121 gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca atggagcgcc
6181 tgccggccca gtatcagccc gtcatacttg aagctagaca ggcttatctt ggacaagaag
6241 aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt tgtccactac gtgaaaggcg
6301 agatcaccaa ggtagtcggc aaataatgtc tagctagaaa ttcgttcaag ccgacgccgc
6361 ttcgccggcg ttaactcaag cgattagatg cactaagcac ataattgctc acagccaaac
6421 tatcaggtca agtctgcttt tattattttt aagcgtgcat aataagccct acacaaattg
6481 ggagatatat catgcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
6541 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
6601 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
6661 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt
6721 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
6781 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
6841 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
6901 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
6961 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
7021 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
7081 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
7141 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
7201 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
7261 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
7321 gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt
7381 atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
7441 cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa
7501 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
7561 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
7621 ggcagggtgc cttgatgtgg gcgccggcgg tcgagtggcg acggcgcggc ttgtccgcgc
7681 cctggtagat tgcctggccg taggccagcc atttttgagc ggccagcggc cgcgataggc
7741 cgacgcgaag cggcggggcg tagggagcgc agcgaccgaa gggtaggcgc tttttgcagc
7801 tcttcggctg tgcgctggcc agacagttat gcacaggcca ggcgggtttt aagagtttta
7861 ataagtttta aagagtttta ggcggaaaaa tcgccttttt tctcttttat atcagtcact
7921 tacatgtgtg accggttccc aatgtacggc tttgggttcc caatgtacgg gttccggttc
7981 ccaatgtacg gctttgggtt cccaatgtac gtgctatcca caggaaagag accttttcga
8041 cctttttccc ctgctagggc aatttgccct agcatctgct ccgtacatta ggaaccggcg
8101 gatgcttcgc cctcgatcag gttgcggtag cgcatgacta ggatcgggcc agcctgcccc
8161 gcctcctcct tcaaatcgta ctccggcagg tcatttgacc cgatcagctt gcgcacggtg
8221 aaacagaact tcttgaactc tccggcgctg ccactgcgtt cgtagatcgt cttgaacaac
8281 catctggctt ctgccttgcc tgcggcgcgg cgtgccaggc ggtagagaaa acggccgatg
8341 ccgggatcga tcaaaaagta atcggggtga accgtcagca cgtccgggtt cttgccttct
8401 gtgatctcgc ggtacatcca atcagctagc tcgatctcga tgtactccgg ccgcccggtt
8461 tcgctcttta cgatcttgta gcggctaatc aaggcttcac cctcggatac cgtcaccagg
8521 cggccgttct tggccttctt cgtacgctgc atggcaacgt gcgtggtgtt taaccgaatg
8581 caggtttcta ccaggtcgtc tttctgcttt ccgccatcgg ctcgccggca gaacttgagt
8641 acgtccgcaa cgtgtggacg gaacacgcgg ccgggcttgt ctcccttccc ttcccggtat
8701 cggttcatgg attcggttag atgggaaacc gccatcagta ccaggtcgta atcccacaca
8761 ctggccatgc cggccggccc tgcggaaacc tctacgtgcc cgtctggaag ctcgtagcgg
8821 atcacctcgc cagctcgtcg gtcacgcttc gacagacgga aaacggccac gtccatgatg
8881 ctgcgactat cgcgggtgcc cacgtcatag agcatcggaa cgaaaaaatc tggttgctcg
8941 tcgcccttgg gcggcttcct aatcgacggc gcaccggctg ccggcggttg ccgggattct
9001 ttgcggattc gatcagcggc cgcttgccac gattcaccgg ggcgtgcttc tgcctcgatg
9061 cgttgccgct gggcggcctg cgcggccttc aacttctcca ccaggtcatc acccagcgcc
9121 gcgccgattt gtaccgggcc ggatggtttg cgaccgtcac gccgattcct cgggcttggg
9181 ggttccagtg ccattgcagg gccggcagac aacccagccg cttacgcctg gccaaccgcc
9241 cgttcctcca cacatggggc attccacggc gtcggtgcct ggttgttctt gattttccat
9301 gccgcctcct ttagccgcta aaattcatct actcatttat tcatttgctc atttactctg
9361 gtagctgcgc gatgtattca gatagcagct cggtaatggt cttgccttgg cgtaccgcgt
9421 acatcttcag cttggtgtga tcctccgccg gcaactgaaa gttgacccgc ttcatggctg
9481 gcgtgtctgc caggctggcc aacgttgcag ccttgctgct gcgtgcgctc ggacggccgg
9541 cacttagcgt gtttgtgctt ttgctcattt tctctttacc tcattaactc aaatgagttt
9601 tgatttaatt tcagcggcca gcgcctggac ctcgcgggca gcgtcgccct cgggttctga
9661 ttcaagaacg gttgtgccgg cggcggcagt gcctgggtag ctcacgcgct gcgtgatacg
9721 ggactcaaga atgggcagct cgtacccggc cagcgcctcg gcaacctcac cgccgatgcg
9781 cgtgcctttg atcgcccgcg acacgacaaa ggccgcttgt agccttccat ccgtgacctc
9841 aatgcgctgc ttaaccagct ccaccaggtc ggcggtggcc catatgtcgt aagggcttgg
9901 ctgcaccgga atcagcacga agtcggctgc cttgatcgcg gacacagcca agtccgccgc
9961 ctggggcgct ccgtcgatca ctacgaagtc gcgccggccg atggccttca cgtcgcggtc
10021 aatcgtcggg cggtcgatgc cgacaacggt tagcggttga tcttcccgca cggccgccca
10081 atcgcgggca ctgccctggg gatcggaatc gactaacaga acatcggccc cggcgagttg
10141 cagggcgcgg gctagatggg ttgcgatggt cgtcttgcct gacccgcctt tctggttaag
10201 tacagcgata accttcatgc gttccccttg cgtatttgtt tatttactca tcgcatcata
10261 tacgcagcga ccgcatgacg caagctgttt tactcaaata cacatcacct ttttagacgg
10321 cggcgctcgg tttcttcagc ggccaagctg gccggccagg ccgccagctt ggcatcagac
10381 aaaccggcca ggatttcatg cagccgcacg gttgagacgt gcgcgggcgg ctcgaacacg
10441 tacccggccg cgatcatctc cgcctcgatc tcttcggtaa tgaaaaacgg ttcgtcctgg
10501 ccgtcctggt gcggtttcat gcttgttcct cttggcgttc attctcggcg gccgccaggg
10561 cgtcggcctc ggtcaatgcg tcctcacgga aggcaccgcg ccgcctggcc tcggtgggcg
10621 tcacttcctc gctgcgctca agtgcgcggt acagggtcga gcgatgcacg ccaagcagtg
10681 cagccgcctc tttcacggtg cggccttcct ggtcgatcag ctcgcgggcg tgcgcgatct
10741 gtgccggggt gagggtaggg cgggggccaa acttcacgcc tcgggccttg gcggcctcgc
10801 gcccgctccg ggtgcggtcg atgattaggg aacgctcgaa ctcggcaatg ccggcgaaca
10861 cggtcaacac catgcggccg gccggcgtgg tggtgtcggc ccacggctct gccaggctac
10921 gcaggcccgc gccggcctcc tggatgcgct cggcaatgtc cagtaggtcg cgggtgctgc
10981 gggccaggcg gtctagcctg gtcactgtca caacgtcgcc agggcgtagg tggtcaagca
11041 tcctggccag ctccgggcgg tcgcgcctgg tgccggtgat cttctcggaa aacagcttgg
11101 tgcagccggc cgcgtgcagt tcggcccgtt ggttggtcaa gtcctggtcg tcggtgctga
11161 cgcgggcata gcccagcagg ccagcggcgg cgctcttgtt catggcgtaa tgtctccggt
11221 tctagtcgca agtattctac tttatgcgac taaaacacgc gacaagaaaa cgccaggaaa
11281 agggcagggc ggcagcctgt cgcgtaactt aggacttgtg cgacatgtcg ttttcagaag
11341 acggctgcac tgaacgtcag aagccgactg cactatagca gcggaggggt tggatcaaag
11401 tactttgatc ccgaggggaa ccctgtggtt ggcatgcaca tacaaatgga cgaacggata
11461 aaccttttca cgccctttta aatatccgtt attctaataa acgctctttt ctcttag
//